4zlh - Revision history

OCA at 12:16, 6 March 2024

OCA — Wed, 06 Mar 2024 12:16:04 GMT

←Older revision		Revision as of 12:16, 6 March 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4zlh]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_O157:H7 Escherichia coli O157:H7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZLH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ZLH FirstGlance]. <br>		<table><tr><td colspan='2'>[[4zlh]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_O157:H7 Escherichia coli O157:H7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZLH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ZLH FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4zlh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zlh OCA], [https://pdbe.org/4zlh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4zlh RCSB], [https://www.ebi.ac.uk/pdbsum/4zlh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4zlh ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4zlh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zlh OCA], [https://pdbe.org/4zlh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4zlh RCSB], [https://www.ebi.ac.uk/pdbsum/4zlh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4zlh ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/LAPB_ECO57 LAPB_ECO57] Modulates cellular lipopolysaccharide (LPS) levels by regulating LpxC, which is involved in lipid A biosynthesis. May act by modulating the proteolytic activity of FtsH towards LpxC. May also coordinate assembly of proteins involved in LPS synthesis at the plasma membrane.[HAMAP-Rule:MF_00994]		[https://www.uniprot.org/uniprot/LAPB_ECO57 LAPB_ECO57] Modulates cellular lipopolysaccharide (LPS) levels by regulating LpxC, which is involved in lipid A biosynthesis. May act by modulating the proteolytic activity of FtsH towards LpxC. May also coordinate assembly of proteins involved in LPS synthesis at the plasma membrane.[HAMAP-Rule:MF_00994]
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Lipopolysaccharide (LPS) synthesis and export are essential pathways for bacterial growth, proliferation, and virulence. The essential protein LapB from Escherichia coli has recently been identified as a regulator of LPS synthesis. We have determined the crystal structure of LapB (without the N-terminal transmembrane helix) at 2 A resolution using zinc single-wavelength anomalous diffraction phasing derived from a single bound zinc atom. This structure demonstrates the presence of nine tetratricopeptide repeats (TPR) motifs, including two TPR folds that were not predicted from sequence, and a rubredoxin-type metal binding domain. The rubredoxin domain is bound intimately to the TPR motifs, which has not been previously observed or predicted. Mutations in the rubredoxin/TPR interface inhibit in vivo cell growth, and in vitro studies indicate that these modifications cause local displacement of rubredoxin from its binding site without changing the secondary structure of LapB. LapB is the first reported structure to contain both a rubredoxin domain and TPR motifs.
-
-	An Unexpected Duo: Rubredoxin Binds Nine TPR Motifs to Form LapB, an Essential Regulator of Lipopolysaccharide Synthesis.,Prince C, Jia Z Structure. 2015 Aug 4;23(8):1500-6. doi: 10.1016/j.str.2015.06.011. Epub 2015 Jul, 16. PMID:26190574<ref>PMID:26190574</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 4zlh" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 07:32, 18 May 2023

OCA — Thu, 18 May 2023 07:32:25 GMT

←Older revision		Revision as of 07:32, 18 May 2023
Line 3:		Line 3:
	<StructureSection load='4zlh' size='340' side='right'caption='[[4zlh]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='4zlh' size='340' side='right'caption='[[4zlh]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4zlh]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Eco57 Eco57~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZLH OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4ZLH FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4zlh]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_O157:H7 Escherichia coli O157:H7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZLH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ZLH FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">lapB, yciM, Z2526, ECs1853 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83334 ECO57])</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4zlh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zlh OCA], [https://pdbe.org/4zlh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4zlh RCSB], [https://www.ebi.ac.uk/pdbsum/4zlh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4zlh ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4zlh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zlh OCA], [~~http~~://pdbe.org/4zlh PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4zlh RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4zlh PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4zlh ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/LAPB_ECO57 LAPB_ECO57]] Modulates cellular lipopolysaccharide (LPS) levels by regulating LpxC, which is involved in lipid A biosynthesis. May act by modulating the proteolytic activity of FtsH towards LpxC. May also coordinate assembly of proteins involved in LPS synthesis at the plasma membrane.[HAMAP-Rule:MF_00994]	+	[https://www.uniprot.org/uniprot/LAPB_ECO57 LAPB_ECO57] Modulates cellular lipopolysaccharide (LPS) levels by regulating LpxC, which is involved in lipid A biosynthesis. May act by modulating the proteolytic activity of FtsH towards LpxC. May also coordinate assembly of proteins involved in LPS synthesis at the plasma membrane.[HAMAP-Rule:MF_00994]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 23:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Eco57~~]]	+	[[Category: Escherichia coli O157:H7]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Jia, Z]]	+	[[Category: Jia Z]]
-	[[Category: Prince~~, C C]]~~	+	[[Category: Prince CC]]
-	~~[[Category: Metal binding protein]]~~	+
-	~~[[Category: Nine tpr motifs rubredoxin lipopolysaccharide regulation~~]]	+

OCA at 07:53, 8 January 2020

OCA — Wed, 08 Jan 2020 07:53:47 GMT

←Older revision		Revision as of 07:53, 8 January 2020
Line 1:		Line 1:

	==Structure of the LapB cytoplasmic domain at 2 angstroms==		==Structure of the LapB cytoplasmic domain at 2 angstroms==
-	<StructureSection load='4zlh' size='340' side='right' caption='[[4zlh]], [[Resolution\|resolution]] 2.00Å' scene=''>	+	<StructureSection load='4zlh' size='340' side='right'caption='[[4zlh]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4zlh]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Eco57 Eco57]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZLH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ZLH FirstGlance]. <br>		<table><tr><td colspan='2'>[[4zlh]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Eco57 Eco57]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZLH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ZLH FirstGlance]. <br>
Line 24:		Line 24:
	</StructureSection>		</StructureSection>
	[[Category: Eco57]]		[[Category: Eco57]]
		+	[[Category: Large Structures]]
	[[Category: Jia, Z]]		[[Category: Jia, Z]]
	[[Category: Prince, C C]]		[[Category: Prince, C C]]
	[[Category: Metal binding protein]]		[[Category: Metal binding protein]]
	[[Category: Nine tpr motifs rubredoxin lipopolysaccharide regulation]]		[[Category: Nine tpr motifs rubredoxin lipopolysaccharide regulation]]

OCA at 13:53, 16 November 2017

OCA — Thu, 16 Nov 2017 13:53:19 GMT

←Older revision		Revision as of 13:53, 16 November 2017
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 4zlh is ~~ON HOLD~~	+	==Structure of the LapB cytoplasmic domain at 2 angstroms==
		+	<StructureSection load='4zlh' size='340' side='right' caption='[[4zlh]], [[Resolution\|resolution]] 2.00Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4zlh]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Eco57 Eco57]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZLH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ZLH FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">lapB, yciM, Z2526, ECs1853 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83334 ECO57])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4zlh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zlh OCA], [http://pdbe.org/4zlh PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4zlh RCSB], [http://www.ebi.ac.uk/pdbsum/4zlh PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4zlh ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/LAPB_ECO57 LAPB_ECO57]] Modulates cellular lipopolysaccharide (LPS) levels by regulating LpxC, which is involved in lipid A biosynthesis. May act by modulating the proteolytic activity of FtsH towards LpxC. May also coordinate assembly of proteins involved in LPS synthesis at the plasma membrane.[HAMAP-Rule:MF_00994]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Lipopolysaccharide (LPS) synthesis and export are essential pathways for bacterial growth, proliferation, and virulence. The essential protein LapB from Escherichia coli has recently been identified as a regulator of LPS synthesis. We have determined the crystal structure of LapB (without the N-terminal transmembrane helix) at 2 A resolution using zinc single-wavelength anomalous diffraction phasing derived from a single bound zinc atom. This structure demonstrates the presence of nine tetratricopeptide repeats (TPR) motifs, including two TPR folds that were not predicted from sequence, and a rubredoxin-type metal binding domain. The rubredoxin domain is bound intimately to the TPR motifs, which has not been previously observed or predicted. Mutations in the rubredoxin/TPR interface inhibit in vivo cell growth, and in vitro studies indicate that these modifications cause local displacement of rubredoxin from its binding site without changing the secondary structure of LapB. LapB is the first reported structure to contain both a rubredoxin domain and TPR motifs.

-	~~Authors~~: ~~Prince~~, C.C., Jia, Z.	+	An Unexpected Duo: Rubredoxin Binds Nine TPR Motifs to Form LapB, an Essential Regulator of Lipopolysaccharide Synthesis.,Prince C, Jia Z Structure. 2015 Aug 4;23(8):1500-6. doi: 10.1016/j.str.2015.06.011. Epub 2015 Jul, 16. PMID:26190574<ref>PMID:26190574</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 4zlh" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Eco57]]
	[[Category: Jia, Z]]		[[Category: Jia, Z]]
-	[[Category: Prince, C.C]]	+	[[Category: Prince, C C]]
		+	[[Category: Metal binding protein]]
		+	[[Category: Nine tpr motifs rubredoxin lipopolysaccharide regulation]]

OCA: Protected "4zlh" [edit=sysop:move=sysop]

OCA — Wed, 13 May 2015 11:05:28 GMT

Protected "4zlh" [edit=sysop:move=sysop]

←Older revision

Revision as of 11:05, 13 May 2015

OCA: New page: '''Unreleased structure''' The entry 4zlh is ON HOLD Authors: Prince, C.C., Jia, Z. Description: Category: Unreleased Structures Category: Jia, Z Category: Prince, C.C

OCA — Wed, 13 May 2015 11:05:26 GMT

New page: '''Unreleased structure''' The entry 4zlh is ON HOLD Authors: Prince, C.C., Jia, Z. Description: Category: Unreleased Structures Category: Jia, Z Category: Prince, C.C

New page

'''Unreleased structure'''

The entry 4zlh is ON HOLD

Authors: Prince, C.C., Jia, Z.

Description:
[[Category: Unreleased Structures]]
[[Category: Jia, Z]]
[[Category: Prince, C.C]]