4zm8 - Revision history

OCA at 07:33, 18 May 2023

OCA — Thu, 18 May 2023 07:33:15 GMT

←Older revision		Revision as of 07:33, 18 May 2023
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	<StructureSection load='4zm8' size='340' side='right'caption='[[4zm8]], [[Resolution\|resolution]] 2.68Å' scene=''>		<StructureSection load='4zm8' size='340' side='right'caption='[[4zm8]], [[Resolution\|resolution]] 2.68Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4zm8]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Black-legged_tick Black-legged tick~~]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3li7 3li7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZM8 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4ZM8 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4zm8]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Ixodes_scapularis Ixodes scapularis]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3li7 3li7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZM8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ZM8 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4zm8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zm8 OCA], [~~http~~://pdbe.org/4zm8 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4zm8 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4zm8 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4zm8 ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4zm8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zm8 OCA], [https://pdbe.org/4zm8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4zm8 RCSB], [https://www.ebi.ac.uk/pdbsum/4zm8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4zm8 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/CYTL_IXOSC CYTL_IXOSC] Inhibitor of cysteine proteinases. Inhibits host immune responses via its inhibition of host cathepsins (PubMed:19494265). Contributes to the suppression of the host's immune response to tick salivary proteins and is important for successful feeding on hosts (PubMed:17698852). Inhibits differentiation of host dendritic cells (PubMed:19494265, PubMed:25975355). Inhibits proliferation of host T-cells in response to antigen stimulus (PubMed:19494265). Down-regulates TLR2-mediated host responses to infection by B.burgdorferi and the production of the chemokine CCL3 by host dendritic cells (PubMed:25975355). Down-regulates host responses to infection by B.burgdorferi and the production of IFNB1 by host dendritic cells (PubMed:25975355). Down-regulates IL1B production by host mast cells, and this then leads to impaired activation of IL1R1, resulting in decreased IL9 production (PubMed:26078269). Inhibits host inflammatory reactions and recruitment of host neutrophils (PubMed:16772304). Inhibits papain and cathepsin-L (CTSL) (in vitro) (PubMed:16772304, PubMed:17698852, PubMed:20545851). Inhibits cathepsin-S (CTSS) (in vitro) (PubMed:17698852, PubMed:20545851). Inhibits CTSV and CTSC, but to a lesser degree (in vitro) (PubMed:16772304, PubMed:17698852).<ref>PMID:16772304</ref> <ref>PMID:17698852</ref> <ref>PMID:19494265</ref> <ref>PMID:20545851</ref> <ref>PMID:25975355</ref> <ref>PMID:26078269</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Black-legged tick~~]]	+	[[Category: Ixodes scapularis]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Andersen~~, J F~~]]	+	[[Category: Andersen JF]]
-	[[Category: Kosyfakis, M]]	+	[[Category: Kosyfakis M]]
-	~~[[Category: Cystatin]]~~	+
-	~~[[Category: Hydrolase inhibitor]]~~	+
-	~~[[Category: Protease inhibitor]]~~	+
-	~~[[Category: Tick~~]]	+

OCA at 08:13, 26 June 2019

OCA — Wed, 26 Jun 2019 08:13:43 GMT

←Older revision		Revision as of 08:13, 26 June 2019
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	==Crystal Structure of Sialostatin L==		==Crystal Structure of Sialostatin L==
-	<StructureSection load='4zm8' size='340' side='right' caption='[[4zm8]], [[Resolution\|resolution]] 2.68Å' scene=''>	+	<StructureSection load='4zm8' size='340' side='right'caption='[[4zm8]], [[Resolution\|resolution]] 2.68Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4zm8]] is a 4 chain structure. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3li7 3li7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZM8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ZM8 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4zm8]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Black-legged_tick Black-legged tick]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3li7 3li7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZM8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ZM8 FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4zm8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zm8 OCA], [http://pdbe.org/4zm8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4zm8 RCSB], [http://www.ebi.ac.uk/pdbsum/4zm8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4zm8 ProSAT]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4zm8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zm8 OCA], [http://pdbe.org/4zm8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4zm8 RCSB], [http://www.ebi.ac.uk/pdbsum/4zm8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4zm8 ProSAT]</span></td></tr>
	</table>		</table>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Black-legged tick]]
		+	[[Category: Large Structures]]
	[[Category: Andersen, J F]]		[[Category: Andersen, J F]]
	[[Category: Kosyfakis, M]]		[[Category: Kosyfakis, M]]

OCA at 17:04, 22 March 2017

OCA — Wed, 22 Mar 2017 17:04:51 GMT

←Older revision		Revision as of 17:04, 22 March 2017
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		+
	==Crystal Structure of Sialostatin L==		==Crystal Structure of Sialostatin L==
	<StructureSection load='4zm8' size='340' side='right' caption='[[4zm8]], [[Resolution\|resolution]] 2.68Å' scene=''>		<StructureSection load='4zm8' size='340' side='right' caption='[[4zm8]], [[Resolution\|resolution]] 2.68Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4zm8]] is a 4 chain structure. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3li7 3li7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZM8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ZM8 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4zm8]] is a 4 chain structure. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3li7 3li7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZM8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ZM8 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4zm8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zm8 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4zm8 RCSB], [http://www.ebi.ac.uk/pdbsum/4zm8 PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4zm8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zm8 OCA], [http://pdbe.org/4zm8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4zm8 RCSB], [http://www.ebi.ac.uk/pdbsum/4zm8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4zm8 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4zm8" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 14:51, 17 June 2015

OCA — Wed, 17 Jun 2015 14:51:30 GMT

←Older revision		Revision as of 14:51, 17 June 2015
Line 1:		Line 1:
-	'''~~Unreleased~~ structure'''	+	==Crystal Structure of Sialostatin L==
		+	<StructureSection load='4zm8' size='340' side='right' caption='[[4zm8]], [[Resolution\|resolution]] 2.68Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4zm8]] is a 4 chain structure. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3li7 3li7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZM8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ZM8 FirstGlance]. <br>
		+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4zm8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zm8 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4zm8 RCSB], [http://www.ebi.ac.uk/pdbsum/4zm8 PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	We have previously demonstrated that two salivary cysteine protease inhibitors from the Borrelia burgdorferi (Lyme disease) vector Ixodes scapularis- namely sialostatins L and L2 - play an important role in tick biology, as demonstrated by the fact that silencing of both sialostatins in tandem results in severe feeding defects. Here we show that sialostatin L2 - but not sialostatin L - facilitates the growth of B. burgdorferi in murine skin. To examine the structural basis underlying these differential effects of the two sialostatins, we have determined the crystal structures of both sialostatin L and L2. This is the first structural analysis of cystatins from an invertebrate source. Sialostatin L2 crystallizes as a monomer with an 'unusual' conformation of the N-terminus, while sialostatin L crystallizes as a domain-swapped dimer with an N-terminal conformation similar to other cystatins. Deletion of the 'unusual' N-terminal five residues of sialostatin L2 results in marked changes in its selectivity, suggesting that this region is a particularly important determinant of the biochemical activity of sialostatin L2. Collectively, our results reveal the structure of two tick salivary components that facilitate vector blood feeding and that one of them also supports pathogen transmission to the vertebrate host.

-	The ~~entry 4zm8 is ON HOLD~~	+	The crystal structures of two salivary cystatins from the tick Ixodes scapularis and the effect of these inhibitors on the establishment of Borrelia burgdorferi infection in a murine model.,Kotsyfakis M, Horka H, Salat J, Andersen JF Mol Microbiol. 2010 Jul;77(2):456-70. Epub 2010 Jun 1. PMID:20545851<ref>PMID:20545851</ref>

-	~~Authors: Andersen~~, J.F.~~, Kosyfakis, M~~.	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~Description: Crystal Structure of Sialostatin L~~	+	== References ==
-	[[Category: ~~Unreleased Structures~~]]	+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Andersen, J F]]
	[[Category: Kosyfakis, M]]		[[Category: Kosyfakis, M]]
-	[[Category: ~~Andersen, J.F~~]]	+	[[Category: Cystatin]]
		+	[[Category: Hydrolase inhibitor]]
		+	[[Category: Protease inhibitor]]
		+	[[Category: Tick]]

OCA: Protected "4zm8" [edit=sysop:move=sysop]

OCA — Wed, 13 May 2015 11:07:21 GMT

Protected "4zm8" [edit=sysop:move=sysop]

←Older revision

Revision as of 11:07, 13 May 2015

OCA: New page: '''Unreleased structure''' The entry 4zm8 is ON HOLD Authors: Andersen, J.F., Kosyfakis, M. Description: Crystal Structure of Sialostatin L Category: Unreleased Structures [[Catego...

OCA — Wed, 13 May 2015 11:07:20 GMT

New page: '''Unreleased structure''' The entry 4zm8 is ON HOLD Authors: Andersen, J.F., Kosyfakis, M. Description: Crystal Structure of Sialostatin L Category: Unreleased Structures [[Catego...

New page

'''Unreleased structure'''

The entry 4zm8 is ON HOLD

Authors: Andersen, J.F., Kosyfakis, M.

Description: Crystal Structure of Sialostatin L
[[Category: Unreleased Structures]]
[[Category: Kosyfakis, M]]
[[Category: Andersen, J.F]]