4zoq - Revision history

OCA at 12:16, 6 March 2024

OCA — Wed, 06 Mar 2024 12:16:18 GMT

←Older revision		Revision as of 12:16, 6 March 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4zoq]] is a 16 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_licheniformis Bacillus licheniformis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZOQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ZOQ FirstGlance]. <br>		<table><tr><td colspan='2'>[[4zoq]] is a 16 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_licheniformis Bacillus licheniformis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZOQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ZOQ FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4zoq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zoq OCA], [https://pdbe.org/4zoq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4zoq RCSB], [https://www.ebi.ac.uk/pdbsum/4zoq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4zoq ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.35Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4zoq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zoq OCA], [https://pdbe.org/4zoq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4zoq RCSB], [https://www.ebi.ac.uk/pdbsum/4zoq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4zoq ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/Q65DC7_BACLD Q65DC7_BACLD]		[https://www.uniprot.org/uniprot/Q65DC7_BACLD Q65DC7_BACLD]
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	The final step of lanthipeptide biosynthesis involves the removal of leader peptides by dedicated proteases. In vitro characterization of LicP, a class II LanP protease involved in the biosynthesis of the lantibiotic lichenicidin, revealed a self-cleavage step that removes 100 amino acids from the N-terminus. The 2.35 A resolution crystal structure provides insights into the active site geometry and substrate specificity, and unveiled an unusual calcium-independent maturation mechanism of a subtilisin family member. LicP processes LicA2 peptides with or without post-translational modifications, but dehydrated and cyclized LicA2 is favored. Investigation of its substrate specificity demonstrated that LicP can serve as an efficient sequence-specific traceless protease and may have great utility in basic research and biotechnology. Encouraged by these findings for LicP, we identified 13 other class II LanPs, ten of which were previously unknown, and suggest that these proteins may serve as a pool of proteases with diverse recognition sequences for general traceless tag removal applications, expanding the current toolbox of proteases.
-
-	Applications of the class II lanthipeptide protease LicP for sequence-specific, traceless peptide bond cleavage.,Tang W, Dong SH, Repka LM, He C, Nair SK, van der Donk WA Chem Sci. 2015 Nov 1;6(11):6270-6279. doi: 10.1039/c5sc02329g. Epub 2015 Sep 2. PMID:30090246<ref>PMID:30090246</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 4zoq" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 07:36, 18 May 2023

OCA — Thu, 18 May 2023 07:36:09 GMT

←Older revision		Revision as of 07:36, 18 May 2023
Line 3:		Line 3:
	<StructureSection load='4zoq' size='340' side='right'caption='[[4zoq]], [[Resolution\|resolution]] 2.35Å' scene=''>		<StructureSection load='4zoq' size='340' side='right'caption='[[4zoq]], [[Resolution\|resolution]] 2.35Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4zoq]] is a 16 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"clostridium_licheniforme"_weigmann_1898 "clostridium licheniforme" weigmann 1898~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZOQ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=4ZOQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4zoq]] is a 16 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_licheniformis Bacillus licheniformis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZOQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ZOQ FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=4zoq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zoq OCA], [~~http~~://pdbe.org/4zoq PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4zoq RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4zoq PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4zoq ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4zoq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zoq OCA], [https://pdbe.org/4zoq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4zoq RCSB], [https://www.ebi.ac.uk/pdbsum/4zoq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4zoq ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q65DC7_BACLD Q65DC7_BACLD]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 19:		Line 21:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Clostridium licheniforme weigmann 1898~~]]	+	[[Category: Bacillus licheniformis]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Dong~~, S H~~]]	+	[[Category: Dong SH]]
-	[[Category: Nair~~, S K]]~~	+	[[Category: Nair SK]]
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Lanthipeptide]]~~	+
-	~~[[Category: Serine protease~~]]	+

OCA at 06:24, 5 August 2020

OCA — Wed, 05 Aug 2020 06:24:14 GMT

←Older revision		Revision as of 06:24, 5 August 2020
Line 1:		Line 1:

	==Crystal Structure of a Lanthipeptide Protease==		==Crystal Structure of a Lanthipeptide Protease==
-	<StructureSection load='4zoq' size='340' side='right' caption='[[4zoq]], [[Resolution\|resolution]] 2.35Å' scene=''>	+	<StructureSection load='4zoq' size='340' side='right'caption='[[4zoq]], [[Resolution\|resolution]] 2.35Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4zoq]] is a 16 chain structure with sequence from [http://en.wikipedia.org/wiki/"clostridium_licheniforme"_weigmann_1898 "clostridium licheniforme" weigmann 1898]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZOQ OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4ZOQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4zoq]] is a 16 chain structure with sequence from [http://en.wikipedia.org/wiki/"clostridium_licheniforme"_weigmann_1898 "clostridium licheniforme" weigmann 1898]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZOQ OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=4ZOQ FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4zoq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zoq OCA], [http://pdbe.org/4zoq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4zoq RCSB], [http://www.ebi.ac.uk/pdbsum/4zoq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4zoq ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=4zoq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zoq OCA], [http://pdbe.org/4zoq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4zoq RCSB], [http://www.ebi.ac.uk/pdbsum/4zoq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4zoq ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 20:		Line 20:
	</StructureSection>		</StructureSection>
	[[Category: Clostridium licheniforme weigmann 1898]]		[[Category: Clostridium licheniforme weigmann 1898]]
		+	[[Category: Large Structures]]
	[[Category: Dong, S H]]		[[Category: Dong, S H]]
	[[Category: Nair, S K]]		[[Category: Nair, S K]]

OCA at 07:04, 31 October 2018

OCA — Wed, 31 Oct 2018 07:04:16 GMT

←Older revision		Revision as of 07:04, 31 October 2018
Line 3:		Line 3:
	<StructureSection load='4zoq' size='340' side='right' caption='[[4zoq]], [[Resolution\|resolution]] 2.35Å' scene=''>		<StructureSection load='4zoq' size='340' side='right' caption='[[4zoq]], [[Resolution\|resolution]] 2.35Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4zoq]] is a 16 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZOQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ZOQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4zoq]] is a 16 chain structure with sequence from [http://en.wikipedia.org/wiki/"clostridium_licheniforme"_weigmann_1898 "clostridium licheniforme" weigmann 1898]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZOQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ZOQ FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4zoq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zoq OCA], [http://pdbe.org/4zoq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4zoq RCSB], [http://www.ebi.ac.uk/pdbsum/4zoq PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4zoq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zoq OCA], [http://pdbe.org/4zoq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4zoq RCSB], [http://www.ebi.ac.uk/pdbsum/4zoq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4zoq ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The final step of lanthipeptide biosynthesis involves the removal of leader peptides by dedicated proteases. In vitro characterization of LicP, a class II LanP protease involved in the biosynthesis of the lantibiotic lichenicidin, revealed a self-cleavage step that removes 100 amino acids from the N-terminus. The 2.35 A resolution crystal structure provides insights into the active site geometry and substrate specificity, and unveiled an unusual calcium-independent maturation mechanism of a subtilisin family member. LicP processes LicA2 peptides with or without post-translational modifications, but dehydrated and cyclized LicA2 is favored. Investigation of its substrate specificity demonstrated that LicP can serve as an efficient sequence-specific traceless protease and may have great utility in basic research and biotechnology. Encouraged by these findings for LicP, we identified 13 other class II LanPs, ten of which were previously unknown, and suggest that these proteins may serve as a pool of proteases with diverse recognition sequences for general traceless tag removal applications, expanding the current toolbox of proteases.
		+
		+	Applications of the class II lanthipeptide protease LicP for sequence-specific, traceless peptide bond cleavage.,Tang W, Dong SH, Repka LM, He C, Nair SK, van der Donk WA Chem Sci. 2015 Nov 1;6(11):6270-6279. doi: 10.1039/c5sc02329g. Epub 2015 Sep 2. PMID:30090246<ref>PMID:30090246</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 4zoq" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Clostridium licheniforme weigmann 1898]]
	[[Category: Dong, S H]]		[[Category: Dong, S H]]
	[[Category: Nair, S K]]		[[Category: Nair, S K]]

OCA at 12:58, 11 May 2016

OCA — Wed, 11 May 2016 12:58:31 GMT

←Older revision		Revision as of 12:58, 11 May 2016
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 4zoq ~~is ON HOLD until Paper Publication~~	+	==Crystal Structure of a Lanthipeptide Protease==
-		+	<StructureSection load='4zoq' size='340' side='right' caption='[[4zoq]], [[Resolution\|resolution]] 2.35Å' scene=''>
-	~~Authors~~: ~~Dong, S~~.H.~~, Nair, S~~.K.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[4zoq]] is a 16 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZOQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ZOQ FirstGlance]. <br>
-	~~Description: Crystal Structure of~~ a ~~Lanthipeptide Protease~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4zoq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zoq OCA], [http://pdbe.org/4zoq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4zoq RCSB], [http://www.ebi.ac.uk/pdbsum/4zoq PDBsum]</span></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	</table>
-	[[Category: Dong, S.H]]	+	__TOC__
-	[[Category: Nair, S.K]]	+	</StructureSection>
		+	[[Category: Dong, S H]]
		+	[[Category: Nair, S K]]
		+	[[Category: Hydrolase]]
		+	[[Category: Lanthipeptide]]
		+	[[Category: Serine protease]]

OCA at 00:07, 1 December 2015

OCA — Tue, 01 Dec 2015 00:07:36 GMT

←Older revision		Revision as of 00:07, 1 December 2015
Line 7:		Line 7:
	Description: Crystal Structure of a Lanthipeptide Protease		Description: Crystal Structure of a Lanthipeptide Protease
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Nair, S.K]]
	[[Category: Dong, S.H]]		[[Category: Dong, S.H]]
		+	[[Category: Nair, S.K]]

OCA at 12:10, 10 June 2015

OCA — Wed, 10 Jun 2015 12:10:47 GMT

←Older revision		Revision as of 12:10, 10 June 2015
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4zoq is ON HOLD	+	The entry 4zoq is ON HOLD until Paper Publication

	Authors: Dong, S.H., Nair, S.K.		Authors: Dong, S.H., Nair, S.K.

OCA: Protected "4zoq" [edit=sysop:move=sysop]

OCA — Wed, 13 May 2015 11:13:20 GMT

Protected "4zoq" [edit=sysop:move=sysop]

←Older revision

Revision as of 11:13, 13 May 2015

OCA: New page: '''Unreleased structure''' The entry 4zoq is ON HOLD Authors: Dong, S.H., Nair, S.K. Description: Crystal Structure of a Lanthipeptide Protease Category: Unreleased Structures [[Ca...

OCA — Wed, 13 May 2015 11:13:19 GMT

New page: '''Unreleased structure''' The entry 4zoq is ON HOLD Authors: Dong, S.H., Nair, S.K. Description: Crystal Structure of a Lanthipeptide Protease Category: Unreleased Structures [[Ca...

New page

'''Unreleased structure'''

The entry 4zoq is ON HOLD

Authors: Dong, S.H., Nair, S.K.

Description: Crystal Structure of a Lanthipeptide Protease
[[Category: Unreleased Structures]]
[[Category: Nair, S.K]]
[[Category: Dong, S.H]]