4zro - Revision history

OCA at 07:39, 18 May 2023

OCA — Thu, 18 May 2023 07:39:11 GMT

←Older revision		Revision as of 07:39, 18 May 2023
Line 3:		Line 3:
	<StructureSection load='4zro' size='340' side='right'caption='[[4zro]], [[Resolution\|resolution]] 2.06Å' scene=''>		<StructureSection load='4zro' size='340' side='right'caption='[[4zro]], [[Resolution\|resolution]] 2.06Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4zro]] is a 8 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Fcov Fcov~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZRO OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4ZRO FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4zro]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Feline_infectious_peritonitis_virus_(strain_79-1146) Feline infectious peritonitis virus (strain 79-1146)] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZRO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ZRO FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"~~><scene name='pdbligand=DMS:DIMETHYL+SULFOXIDE'>DMS</scene></td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BOC:TERT-BUTYL+HYDROGEN+CARBONATE'>BOC</scene>, <scene name='pdbligand=CEV:ETHYL+(4R)-4-AMINO-5-[(3S)-2-OXOPYRROLIDIN-3-YL]PENTANOATE'>CEV</scene>, <scene name='pdbligand=DMS:DIMETHYL+SULFOXIDE'>DMS</scene></td></tr>
-	~~<tr~~ id~~='NonStdRes'><td class~~="~~sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat~~"><scene name='pdbligand=BOC:TERT-BUTYL+HYDROGEN+CARBONATE'>BOC</scene>, <scene name='pdbligand=CEV:ETHYL+(4R)-4-AMINO-5-[(3S)-2-OXOPYRROLIDIN-3-YL]PENTANOATE'>CEV</scene><~~/td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4zro FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zro OCA], [https://pdbe.org/4zro PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4zro RCSB], [https://www.ebi.ac.uk/pdbsum/4zro PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4zro ProSAT]</span></td></tr>
-	~~<tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b~~></td>~~<td class="sblockDat">rep, 1a-1b ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=33734 FCoV])~~</td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4zro FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zro OCA], [~~http~~://pdbe.org/4zro PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4zro RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4zro PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4zro ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/R1AB_FIPV R1AB_FIPV]] The replicase polyprotein of coronaviruses is a multifunctional protein: it contains the activities necessary for the transcription of negative stranded RNA, leader RNA, subgenomic mRNAs and progeny virion RNA as well as proteinases responsible for the cleavage of the polyprotein into functional products. The papain-like proteinase 1 (PLP1) and papain-like proteinase 2 (PLP2) are responsible for the cleavages located at the N-terminus of the replicase polyprotein. In addition, PLP2 possesses a deubiquitinating/deISGylating activity and processes both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains from cellular substrates. PLP2 also antagonizes innate immune induction of type I interferon by blocking the nuclear translocation of host IRF-3 (By similarity). The main proteinase 3CL-PRO is responsible for the majority of cleavages as it cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-\|-[SGACN]. Inhibited by the substrate-analog Cbz-Val-Asn-Ser-Thr-Leu-Gln-CMK. Also contains an ADP-ribose-1''-phosphate (ADRP)-binding function (By similarity). The helicase which contains a zinc finger structure displays RNA and DNA duplex-unwinding activities with 5' to 3' polarity. ATPase activity is strongly stimulated by poly(U), poly(dT), poly(C), poly(dA), but not by poly(G) (By similarity). The exoribonuclease acts on both ssRNA and dsRNA in a 3' to 5' direction (By similarity). Nsp7-nsp8 hexadecamer may possibly confer processivity to the polymerase, maybe by binding to dsRNA or by producing primers utilized by the latter (By similarity). Nsp9 is a ssRNA-binding protein (By similarity). NendoU is a Mn(2+)-dependent, uridylate-specific enzyme, which leaves 2'-3'-cyclic phosphates 5' to the cleaved bond (By similarity).	+	[https://www.uniprot.org/uniprot/R1AB_FIPV R1AB_FIPV] The replicase polyprotein of coronaviruses is a multifunctional protein: it contains the activities necessary for the transcription of negative stranded RNA, leader RNA, subgenomic mRNAs and progeny virion RNA as well as proteinases responsible for the cleavage of the polyprotein into functional products. The papain-like proteinase 1 (PLP1) and papain-like proteinase 2 (PLP2) are responsible for the cleavages located at the N-terminus of the replicase polyprotein. In addition, PLP2 possesses a deubiquitinating/deISGylating activity and processes both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains from cellular substrates. PLP2 also antagonizes innate immune induction of type I interferon by blocking the nuclear translocation of host IRF-3 (By similarity). The main proteinase 3CL-PRO is responsible for the majority of cleavages as it cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-\|-[SGACN]. Inhibited by the substrate-analog Cbz-Val-Asn-Ser-Thr-Leu-Gln-CMK. Also contains an ADP-ribose-1''-phosphate (ADRP)-binding function (By similarity). The helicase which contains a zinc finger structure displays RNA and DNA duplex-unwinding activities with 5' to 3' polarity. ATPase activity is strongly stimulated by poly(U), poly(dT), poly(C), poly(dA), but not by poly(G) (By similarity). The exoribonuclease acts on both ssRNA and dsRNA in a 3' to 5' direction (By similarity). Nsp7-nsp8 hexadecamer may possibly confer processivity to the polymerase, maybe by binding to dsRNA or by producing primers utilized by the latter (By similarity). Nsp9 is a ssRNA-binding protein (By similarity). NendoU is a Mn(2+)-dependent, uridylate-specific enzyme, which leaves 2'-3'-cyclic phosphates 5' to the cleaved bond (By similarity).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 22:		Line 20:

	==See Also==		==See Also==
-	*[[~~SARS Coronavirus Main Proteinase~~\|~~SARS Coronavirus Main Proteinase~~]]	+	*[[Virus protease 3D structures\|Virus protease 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Fcov]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~John, S E.St~~]]	+	[[Category: Synthetic construct]]
-	[[Category: Mesecar~~, A D~~]]	+	[[Category: Mesecar AD]]
-	[[Category: ~~Coronavirus]]~~	+	[[Category: St John SE]]
-	~~[[Category: Feline infectious peritonitis]]~~	+
-	~~[[Category: Fipv]]~~	+
-	~~[[Category: Hydrolase-hydrolase inhibitor complex]]~~	+
-	~~[[Category: Inhibitor complex]]~~	+
-	~~[[Category: Main protease]]~~	+
-	~~[[Category: Mpro~~]]	+

OCA at 15:56, 11 December 2019

OCA — Wed, 11 Dec 2019 15:56:23 GMT

←Older revision		Revision as of 15:56, 11 December 2019
Line 1:		Line 1:

	==2.1 A X-Ray Structure of FIPV-3CLpro bound to covalent inhibitor==		==2.1 A X-Ray Structure of FIPV-3CLpro bound to covalent inhibitor==
-	<StructureSection load='4zro' size='340' side='right' caption='[[4zro]], [[Resolution\|resolution]] 2.06Å' scene=''>	+	<StructureSection load='4zro' size='340' side='right'caption='[[4zro]], [[Resolution\|resolution]] 2.06Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4zro]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Fcov Fcov]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZRO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ZRO FirstGlance]. <br>		<table><tr><td colspan='2'>[[4zro]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Fcov Fcov]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZRO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ZRO FirstGlance]. <br>
Line 20:		Line 20:
	</div>		</div>
	<div class="pdbe-citations 4zro" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 4zro" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[SARS Coronavirus Main Proteinase\|SARS Coronavirus Main Proteinase]]
	== References ==		== References ==
	<references/>		<references/>
Line 25:		Line 28:
	</StructureSection>		</StructureSection>
	[[Category: Fcov]]		[[Category: Fcov]]
		+	[[Category: Large Structures]]
	[[Category: John, S E.St]]		[[Category: John, S E.St]]
	[[Category: Mesecar, A D]]		[[Category: Mesecar, A D]]

OCA at 16:13, 15 November 2017

OCA — Wed, 15 Nov 2017 16:13:12 GMT

←Older revision		Revision as of 16:13, 15 November 2017
Line 1:		Line 1:
		+
	==2.1 A X-Ray Structure of FIPV-3CLpro bound to covalent inhibitor==		==2.1 A X-Ray Structure of FIPV-3CLpro bound to covalent inhibitor==
	<StructureSection load='4zro' size='340' side='right' caption='[[4zro]], [[Resolution\|resolution]] 2.06Å' scene=''>		<StructureSection load='4zro' size='340' side='right' caption='[[4zro]], [[Resolution\|resolution]] 2.06Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4zro]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZRO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ZRO FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4zro]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Fcov Fcov]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZRO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ZRO FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DMS:DIMETHYL+SULFOXIDE'>DMS</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DMS:DIMETHYL+SULFOXIDE'>DMS</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=BOC:TERT-BUTYL+HYDROGEN+CARBONATE'>BOC</scene>, <scene name='pdbligand=CEV:ETHYL+(4R)-4-AMINO-5-[(3S)-2-OXOPYRROLIDIN-3-YL]PENTANOATE'>CEV</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=BOC:TERT-BUTYL+HYDROGEN+CARBONATE'>BOC</scene>, <scene name='pdbligand=CEV:ETHYL+(4R)-4-AMINO-5-[(3S)-2-OXOPYRROLIDIN-3-YL]PENTANOATE'>CEV</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4zro FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zro OCA], [http://pdbe.org/4zro PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4zro RCSB], [http://www.ebi.ac.uk/pdbsum/4zro PDBsum]</span></td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">rep, 1a-1b ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=33734 FCoV])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4zro FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zro OCA], [http://pdbe.org/4zro PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4zro RCSB], [http://www.ebi.ac.uk/pdbsum/4zro PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4zro ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 22:		Line 24:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Fcov]]
	[[Category: John, S E.St]]		[[Category: John, S E.St]]
	[[Category: Mesecar, A D]]		[[Category: Mesecar, A D]]
	[[Category: Coronavirus]]		[[Category: Coronavirus]]
-	[[Category: Fcov]]
	[[Category: Feline infectious peritonitis]]		[[Category: Feline infectious peritonitis]]
	[[Category: Fipv]]		[[Category: Fipv]]

OCA at 07:30, 9 December 2015

OCA — Wed, 09 Dec 2015 07:30:26 GMT

←Older revision		Revision as of 07:30, 9 December 2015
Line 9:		Line 9:
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/R1AB_FIPV R1AB_FIPV]] The replicase polyprotein of coronaviruses is a multifunctional protein: it contains the activities necessary for the transcription of negative stranded RNA, leader RNA, subgenomic mRNAs and progeny virion RNA as well as proteinases responsible for the cleavage of the polyprotein into functional products. The papain-like proteinase 1 (PLP1) and papain-like proteinase 2 (PLP2) are responsible for the cleavages located at the N-terminus of the replicase polyprotein. In addition, PLP2 possesses a deubiquitinating/deISGylating activity and processes both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains from cellular substrates. PLP2 also antagonizes innate immune induction of type I interferon by blocking the nuclear translocation of host IRF-3 (By similarity). The main proteinase 3CL-PRO is responsible for the majority of cleavages as it cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-\|-[SGACN]. Inhibited by the substrate-analog Cbz-Val-Asn-Ser-Thr-Leu-Gln-CMK. Also contains an ADP-ribose-1''-phosphate (ADRP)-binding function (By similarity). The helicase which contains a zinc finger structure displays RNA and DNA duplex-unwinding activities with 5' to 3' polarity. ATPase activity is strongly stimulated by poly(U), poly(dT), poly(C), poly(dA), but not by poly(G) (By similarity). The exoribonuclease acts on both ssRNA and dsRNA in a 3' to 5' direction (By similarity). Nsp7-nsp8 hexadecamer may possibly confer processivity to the polymerase, maybe by binding to dsRNA or by producing primers utilized by the latter (By similarity). Nsp9 is a ssRNA-binding protein (By similarity). NendoU is a Mn(2+)-dependent, uridylate-specific enzyme, which leaves 2'-3'-cyclic phosphates 5' to the cleaved bond (By similarity).		[[http://www.uniprot.org/uniprot/R1AB_FIPV R1AB_FIPV]] The replicase polyprotein of coronaviruses is a multifunctional protein: it contains the activities necessary for the transcription of negative stranded RNA, leader RNA, subgenomic mRNAs and progeny virion RNA as well as proteinases responsible for the cleavage of the polyprotein into functional products. The papain-like proteinase 1 (PLP1) and papain-like proteinase 2 (PLP2) are responsible for the cleavages located at the N-terminus of the replicase polyprotein. In addition, PLP2 possesses a deubiquitinating/deISGylating activity and processes both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains from cellular substrates. PLP2 also antagonizes innate immune induction of type I interferon by blocking the nuclear translocation of host IRF-3 (By similarity). The main proteinase 3CL-PRO is responsible for the majority of cleavages as it cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-\|-[SGACN]. Inhibited by the substrate-analog Cbz-Val-Asn-Ser-Thr-Leu-Gln-CMK. Also contains an ADP-ribose-1''-phosphate (ADRP)-binding function (By similarity). The helicase which contains a zinc finger structure displays RNA and DNA duplex-unwinding activities with 5' to 3' polarity. ATPase activity is strongly stimulated by poly(U), poly(dT), poly(C), poly(dA), but not by poly(G) (By similarity). The exoribonuclease acts on both ssRNA and dsRNA in a 3' to 5' direction (By similarity). Nsp7-nsp8 hexadecamer may possibly confer processivity to the polymerase, maybe by binding to dsRNA or by producing primers utilized by the latter (By similarity). Nsp9 is a ssRNA-binding protein (By similarity). NendoU is a Mn(2+)-dependent, uridylate-specific enzyme, which leaves 2'-3'-cyclic phosphates 5' to the cleaved bond (By similarity).
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Feline infectious peritonitis (FIP) is a deadly disease that effects both domestic and wild cats and is caused by a mutation in feline coronavirus (FCoV) that allows the virus to replicate in macrophages. Currently, there are no treatments or vaccines available for the treatment of FIP even though it kills approximately 5% of cats in multi-cat households per year. In an effort to develop small molecule drugs targeting FIP for the treatment of cats, we screened a small set of designed peptidomimetic inhibitors for inhibition of FIPV-3CL(pro), identifying two compounds with low to sub-micromolar inhibition, compound 6 (IC50=0.59+/-0.06muM) and compound 7 (IC50=1.3+/-0.1muM). We determined the first X-ray crystal structure of FIPV-3CL(pro) in complex with the best inhibitor identified, compound 6, to a resolution of 2.10A to better understand the structural basis for inhibitor specificity. Our study provides important insights into the structural requirements for the inhibition of FIPV-3CL(pro) by peptidomimetic inhibitors and expands the current structural knowledge of coronaviral 3CL(pro) architecture.
		+
		+	X-ray structure and inhibition of the feline infectious peritonitis virus 3C-like protease: Structural implications for drug design.,St John SE, Therkelsen MD, Nyalapatla PR, Osswald HL, Ghosh AK, Mesecar AD Bioorg Med Chem Lett. 2015 Nov 15;25(22):5072-7. doi: 10.1016/j.bmcl.2015.10.023., Epub 2015 Oct 13. PMID:26592814<ref>PMID:26592814</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 4zro" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: John, S E.St]]
	[[Category: Mesecar, A D]]		[[Category: Mesecar, A D]]
-	[[Category: St John, S E]]
	[[Category: Coronavirus]]		[[Category: Coronavirus]]
	[[Category: Fcov]]		[[Category: Fcov]]

OCA at 04:05, 16 October 2015

OCA — Fri, 16 Oct 2015 04:05:43 GMT

←Older revision		Revision as of 04:05, 16 October 2015
Line 1:		Line 1:
-	'''~~Unreleased structure~~'''	+	==2.1 A X-Ray Structure of FIPV-3CLpro bound to covalent inhibitor==
-		+	<StructureSection load='4zro' size='340' side='right' caption='[[4zro]], [[Resolution\|resolution]] 2.06Å' scene=''>
-	~~The entry~~ 4zro is ~~ON HOLD~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[4zro]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZRO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ZRO FirstGlance]. <br>
-	~~Authors~~: St. ~~John~~, S.E., ~~Mesecar~~, A.D.	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DMS:DIMETHYL+SULFOXIDE'>DMS</scene></td></tr>
-		+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=BOC:TERT-BUTYL+HYDROGEN+CARBONATE'>BOC</scene>, <scene name='pdbligand=CEV:ETHYL+(4R)-4-AMINO-5-[(3S)-2-OXOPYRROLIDIN-3-YL]PENTANOATE'>CEV</scene></td></tr>
-	~~Description~~: 2.1 ~~A X~~-~~Ray Structure~~ of ~~FIPV~~-~~3CLpro bound~~ to ~~covalent inhibitor~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4zro FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zro OCA], [http://pdbe.org/4zro PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4zro RCSB], [http://www.ebi.ac.uk/pdbsum/4zro PDBsum]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
-	[[Category: St. John, S.E]]	+	== Function ==
-	[[Category: ~~Mesecar, A.D~~]]	+	[[http://www.uniprot.org/uniprot/R1AB_FIPV R1AB_FIPV]] The replicase polyprotein of coronaviruses is a multifunctional protein: it contains the activities necessary for the transcription of negative stranded RNA, leader RNA, subgenomic mRNAs and progeny virion RNA as well as proteinases responsible for the cleavage of the polyprotein into functional products. The papain-like proteinase 1 (PLP1) and papain-like proteinase 2 (PLP2) are responsible for the cleavages located at the N-terminus of the replicase polyprotein. In addition, PLP2 possesses a deubiquitinating/deISGylating activity and processes both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains from cellular substrates. PLP2 also antagonizes innate immune induction of type I interferon by blocking the nuclear translocation of host IRF-3 (By similarity). The main proteinase 3CL-PRO is responsible for the majority of cleavages as it cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-\|-[SGACN]. Inhibited by the substrate-analog Cbz-Val-Asn-Ser-Thr-Leu-Gln-CMK. Also contains an ADP-ribose-1''-phosphate (ADRP)-binding function (By similarity). The helicase which contains a zinc finger structure displays RNA and DNA duplex-unwinding activities with 5' to 3' polarity. ATPase activity is strongly stimulated by poly(U), poly(dT), poly(C), poly(dA), but not by poly(G) (By similarity). The exoribonuclease acts on both ssRNA and dsRNA in a 3' to 5' direction (By similarity). Nsp7-nsp8 hexadecamer may possibly confer processivity to the polymerase, maybe by binding to dsRNA or by producing primers utilized by the latter (By similarity). Nsp9 is a ssRNA-binding protein (By similarity). NendoU is a Mn(2+)-dependent, uridylate-specific enzyme, which leaves 2'-3'-cyclic phosphates 5' to the cleaved bond (By similarity).
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Mesecar, A D]]
		+	[[Category: St John, S E]]
		+	[[Category: Coronavirus]]
		+	[[Category: Fcov]]
		+	[[Category: Feline infectious peritonitis]]
		+	[[Category: Fipv]]
		+	[[Category: Hydrolase-hydrolase inhibitor complex]]
		+	[[Category: Inhibitor complex]]
		+	[[Category: Main protease]]
		+	[[Category: Mpro]]

OCA at 14:38, 27 May 2015

OCA — Wed, 27 May 2015 14:38:51 GMT

←Older revision		Revision as of 14:38, 27 May 2015
Line 3:		Line 3:
	The entry 4zro is ON HOLD		The entry 4zro is ON HOLD

-	Authors: St.John, S.E., Mesecar, A.D.	+	Authors: St. John, S.E., Mesecar, A.D.

	Description: 2.1 A X-Ray Structure of FIPV-3CLpro bound to covalent inhibitor		Description: 2.1 A X-Ray Structure of FIPV-3CLpro bound to covalent inhibitor
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: St.John, S.E]]	+	[[Category: St. John, S.E]]
	[[Category: Mesecar, A.D]]		[[Category: Mesecar, A.D]]

OCA: Protected "4zro" [edit=sysop:move=sysop]

OCA — Wed, 20 May 2015 11:04:24 GMT

Protected "4zro" [edit=sysop:move=sysop]

←Older revision

Revision as of 11:04, 20 May 2015

OCA: New page: '''Unreleased structure''' The entry 4zro is ON HOLD Authors: St.John, S.E., Mesecar, A.D. Description: 2.1 A X-Ray Structure of FIPV-3CLpro bound to covalent inhibitor [[Category: Unr...

OCA — Wed, 20 May 2015 11:04:23 GMT

New page: '''Unreleased structure''' The entry 4zro is ON HOLD Authors: St.John, S.E., Mesecar, A.D. Description: 2.1 A X-Ray Structure of FIPV-3CLpro bound to covalent inhibitor [[Category: Unr...

New page

'''Unreleased structure'''

The entry 4zro is ON HOLD

Authors: St.John, S.E., Mesecar, A.D.

Description: 2.1 A X-Ray Structure of FIPV-3CLpro bound to covalent inhibitor
[[Category: Unreleased Structures]]
[[Category: St.John, S.E]]
[[Category: Mesecar, A.D]]