4zum - Revision history

OCA at 08:24, 27 September 2023

OCA — Wed, 27 Sep 2023 08:24:31 GMT

←Older revision		Revision as of 08:24, 27 September 2023
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4zum]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycoplana_ramosa Mycoplana ramosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZUM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ZUM FirstGlance]. <br>		<table><tr><td colspan='2'>[[4zum]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycoplana_ramosa Mycoplana ramosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZUM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ZUM FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FKS:7-[(3-AMINOPROPYL)AMINO]-1,1,1-TRIFLUOROHEPTANE-2,2-DIOL'>FKS</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.42Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FKS:7-[(3-AMINOPROPYL)AMINO]-1,1,1-TRIFLUOROHEPTANE-2,2-DIOL'>FKS</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4zum FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zum OCA], [https://pdbe.org/4zum PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4zum RCSB], [https://www.ebi.ac.uk/pdbsum/4zum PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4zum ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4zum FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zum OCA], [https://pdbe.org/4zum PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4zum RCSB], [https://www.ebi.ac.uk/pdbsum/4zum PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4zum ProSAT]</span></td></tr>
	</table>		</table>

OCA at 07:42, 18 May 2023

OCA — Thu, 18 May 2023 07:42:20 GMT

←Older revision		Revision as of 07:42, 18 May 2023
Line 3:		Line 3:
	<StructureSection load='4zum' size='340' side='right'caption='[[4zum]], [[Resolution\|resolution]] 1.42Å' scene=''>		<StructureSection load='4zum' size='340' side='right'caption='[[4zum]], [[Resolution\|resolution]] 1.42Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4zum]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Atcc_49678 Atcc 49678~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZUM OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4ZUM FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4zum]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycoplana_ramosa Mycoplana ramosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZUM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ZUM FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FKS:7-[(3-AMINOPROPYL)AMINO]-1,1,1-TRIFLUOROHEPTANE-2,2-DIOL'>FKS</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FKS:7-[(3-AMINOPROPYL)AMINO]-1,1,1-TRIFLUOROHEPTANE-2,2-DIOL'>FKS</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4zun\|4zun]], [[4zuo\|4zuo]], [[4zup\|4zup]], [[4zuq\|4zuq]], [[4zur\|4zur]]</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4zum FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zum OCA], [https://pdbe.org/4zum PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4zum RCSB], [https://www.ebi.ac.uk/pdbsum/4zum PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4zum ProSAT]</span></td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">aphA, aph ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=40837 ATCC 49678])~~</td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4zum FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zum OCA], [~~http~~://pdbe.org/4zum PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4zum RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4zum PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4zum ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/~~APHA_MYCRA APHA_MYCRA~~]~~] Acts on many types of acetylpolyamines~~. ~~Has high affinity towards~~ acetylputrescine, acetylcadaverine, ~~acetylspermidine~~, and ~~acetylspermine~~. ~~Acts on~~ L-Lys-(epsilon-acetyl)-coumarin, but has very low activity towards ~~acetylated peptides~~.	+	[https://www.uniprot.org/uniprot/APAH_MYCRA APAH_MYCRA] Involved in polyamine metabolism. Catalyzes the deacetylation of various acetylated polyamines such as N-acetylputrescine, N-acetylcadaverine, N(1)-acetylspermine, N(1)-acetylspermidine and N(8)-acetylspermidine (PubMed:8824626, PubMed:3207420). In vitro, is also able to deacetylate L-Lys(epsilon-acetyl)coumarin, but has very low activity towards the larger tetrapeptide N-acetyl-L-Arg-L-His-L-Lys(epsilon-acetyl)-L-Lys(epsilon-acetyl)coumarin (PubMed:21268586).<ref>PMID:21268586</ref> <ref>PMID:3207420</ref> <ref>PMID:8824626</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 24:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Atcc 49678]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Christianson, D W~~]]	+	[[Category: Mycoplana ramosa]]
-	[[Category: ~~Decroos, C~~]]	+	[[Category: Christianson DW]]
-	[[Category: ~~Acetylpolyamine amidohydrolase]]~~	+	[[Category: Decroos C]]
-	~~[[Category: Arginase fold]]~~	+
-	~~[[Category: Enzyme-inhibitor complex]]~~	+
-	~~[[Category: Hydrolase-hydrolase inhibitor complex]]~~	+
-	~~[[Category: Polyamine~~]]	+

OCA at 11:02, 25 December 2019

OCA — Wed, 25 Dec 2019 11:02:28 GMT

←Older revision		Revision as of 11:02, 25 December 2019
Line 3:		Line 3:
	<StructureSection load='4zum' size='340' side='right'caption='[[4zum]], [[Resolution\|resolution]] 1.42Å' scene=''>		<StructureSection load='4zum' size='340' side='right'caption='[[4zum]], [[Resolution\|resolution]] 1.42Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4zum]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZUM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ZUM FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4zum]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_49678 Atcc 49678]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZUM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ZUM FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FKS:7-[(3-AMINOPROPYL)AMINO]-1,1,1-TRIFLUOROHEPTANE-2,2-DIOL'>FKS</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FKS:7-[(3-AMINOPROPYL)AMINO]-1,1,1-TRIFLUOROHEPTANE-2,2-DIOL'>FKS</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4zun\|4zun]], [[4zuo\|4zuo]], [[4zup\|4zup]], [[4zuq\|4zuq]], [[4zur\|4zur]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4zun\|4zun]], [[4zuo\|4zuo]], [[4zup\|4zup]], [[4zuq\|4zuq]], [[4zur\|4zur]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">aphA, aph ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=40837 ATCC 49678])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4zum FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zum OCA], [http://pdbe.org/4zum PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4zum RCSB], [http://www.ebi.ac.uk/pdbsum/4zum PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4zum ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4zum FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zum OCA], [http://pdbe.org/4zum PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4zum RCSB], [http://www.ebi.ac.uk/pdbsum/4zum PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4zum ProSAT]</span></td></tr>
	</table>		</table>
Line 23:		Line 24:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Atcc 49678]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Christianson, D W]]		[[Category: Christianson, D W]]

OCA at 07:25, 10 July 2019

OCA — Wed, 10 Jul 2019 07:25:30 GMT

←Older revision		Revision as of 07:25, 10 July 2019
Line 1:		Line 1:

	==Crystal structure of acetylpolyamine amidohydrolase from Mycoplana ramosa in complex with a trifluoromethylketone inhibitor==		==Crystal structure of acetylpolyamine amidohydrolase from Mycoplana ramosa in complex with a trifluoromethylketone inhibitor==
-	<StructureSection load='4zum' size='340' side='right' caption='[[4zum]], [[Resolution\|resolution]] 1.42Å' scene=''>	+	<StructureSection load='4zum' size='340' side='right'caption='[[4zum]], [[Resolution\|resolution]] 1.42Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4zum]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZUM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ZUM FirstGlance]. <br>		<table><tr><td colspan='2'>[[4zum]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZUM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ZUM FirstGlance]. <br>
Line 23:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Christianson, D W]]		[[Category: Christianson, D W]]
	[[Category: Decroos, C]]		[[Category: Decroos, C]]

OCA at 16:08, 17 May 2017

OCA — Wed, 17 May 2017 16:08:45 GMT

←Older revision		Revision as of 16:08, 17 May 2017
Line 1:		Line 1:
		+
	==Crystal structure of acetylpolyamine amidohydrolase from Mycoplana ramosa in complex with a trifluoromethylketone inhibitor==		==Crystal structure of acetylpolyamine amidohydrolase from Mycoplana ramosa in complex with a trifluoromethylketone inhibitor==
	<StructureSection load='4zum' size='340' side='right' caption='[[4zum]], [[Resolution\|resolution]] 1.42Å' scene=''>		<StructureSection load='4zum' size='340' side='right' caption='[[4zum]], [[Resolution\|resolution]] 1.42Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FKS:7-[(3-AMINOPROPYL)AMINO]-1,1,1-TRIFLUOROHEPTANE-2,2-DIOL'>FKS</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FKS:7-[(3-AMINOPROPYL)AMINO]-1,1,1-TRIFLUOROHEPTANE-2,2-DIOL'>FKS</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4zun\|4zun]], [[4zuo\|4zuo]], [[4zup\|4zup]], [[4zuq\|4zuq]], [[4zur\|4zur]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4zun\|4zun]], [[4zuo\|4zuo]], [[4zup\|4zup]], [[4zuq\|4zuq]], [[4zur\|4zur]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4zum FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zum OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4zum RCSB], [http://www.ebi.ac.uk/pdbsum/4zum PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4zum FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zum OCA], [http://pdbe.org/4zum PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4zum RCSB], [http://www.ebi.ac.uk/pdbsum/4zum PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4zum ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 17:		Line 18:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4zum" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 13:44, 5 August 2015

OCA — Wed, 05 Aug 2015 13:44:30 GMT

←Older revision		Revision as of 13:44, 5 August 2015
Line 9:		Line 9:
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/APHA_MYCRA APHA_MYCRA]] Acts on many types of acetylpolyamines. Has high affinity towards acetylputrescine, acetylcadaverine, acetylspermidine, and acetylspermine. Acts on L-Lys-(epsilon-acetyl)-coumarin, but has very low activity towards acetylated peptides.		[[http://www.uniprot.org/uniprot/APHA_MYCRA APHA_MYCRA]] Acts on many types of acetylpolyamines. Has high affinity towards acetylputrescine, acetylcadaverine, acetylspermidine, and acetylspermine. Acts on L-Lys-(epsilon-acetyl)-coumarin, but has very low activity towards acetylated peptides.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Polyamines are essential aliphatic polycations that bind to nucleic acids and accordingly are involved in a variety of cellular processes. Polyamine function can be regulated by acetylation and deacetylation, just as histone function can be regulated by lysine acetylation and deacetylation. Acetylpolyamine amidohydrolase (APAH) from Mycoplana ramosa is a zinc-dependent polyamine deacetylase that shares approximately 20% amino acid sequence identity with human histone deacetylases. We now report the X-ray crystal structures of APAH-inhibitor complexes in a new and superior crystal form that diffracts to very high resolution (1.1-1.4 A). Inhibitors include previously synthesized analogues of N(8)-acetylspermidine bearing trifluoromethylketone, thiol, and hydroxamate zinc-binding groups [Decroos, C., Bowman, C. M., and Christianson, D. W. (2013) Bioorg. Med. Chem. 21, 4530], and newly synthesized hydroxamate analogues of shorter, monoacetylated diamines, the most potent of which is the hydroxamate analogue of N-acetylcadaverine (IC50 = 68 nM). The high-resolution crystal structures of APAH-inhibitor complexes provide key inferences about the inhibition and catalytic mechanism of zinc-dependent deacetylases. For example, the trifluoromethylketone analogue of N(8)-acetylspermidine binds as a tetrahedral gem-diol that mimics the tetrahedral intermediate and its flanking transition states in catalysis. Surprisingly, this compound is also a potent inhibitor of human histone deacetylase 8 with an IC50 of 260 nM. Crystal structures of APAH-inhibitor complexes are determined at the highest resolution of any currently existing zinc deacetylase structure and thus represent the most accurate reference points for understanding structure-mechanism and structure-inhibition relationships in this critically important enzyme family.
		+
		+	Design, Synthesis, and Evaluation of Polyamine Deacetylase Inhibitors, and High-Resolution Crystal Structures of Their Complexes with Acetylpolyamine Amidohydrolase.,Decroos C, Christianson DW Biochemistry. 2015 Aug 4;54(30):4692-703. doi: 10.1021/acs.biochem.5b00536. Epub , 2015 Jul 22. PMID:26200446<ref>PMID:26200446</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 14:52, 29 July 2015

OCA — Wed, 29 Jul 2015 14:52:37 GMT

←Older revision		Revision as of 14:52, 29 July 2015
Line 1:		Line 1:
-	'''~~Unreleased structure~~'''	+	==Crystal structure of acetylpolyamine amidohydrolase from Mycoplana ramosa in complex with a trifluoromethylketone inhibitor==
-		+	<StructureSection load='4zum' size='340' side='right' caption='[[4zum]], [[Resolution\|resolution]] 1.42Å' scene=''>
-	~~The entry~~ 4zum is ~~ON HOLD until Paper Publication~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[4zum]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZUM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ZUM FirstGlance]. <br>
-	~~Authors~~: ~~Decroos~~, C., ~~Christianson~~, D.W.	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FKS:7-[(3-AMINOPROPYL)AMINO]-1,1,1-TRIFLUOROHEPTANE-2,2-DIOL'>FKS</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4zun\|4zun]], [[4zuo\|4zuo]], [[4zup\|4zup]], [[4zuq\|4zuq]], [[4zur\|4zur]]</td></tr>
-	~~Description~~: ~~Crystal structure~~ of ~~acetylpolyamine amidohydrolase from Mycoplana ramosa in complex with a trifluoromethylketone inhibitor~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4zum FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zum OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4zum RCSB], [http://www.ebi.ac.uk/pdbsum/4zum PDBsum]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/APHA_MYCRA APHA_MYCRA]] Acts on many types of acetylpolyamines. Has high affinity towards acetylputrescine, acetylcadaverine, acetylspermidine, and acetylspermine. Acts on L-Lys-(epsilon-acetyl)-coumarin, but has very low activity towards acetylated peptides.
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Christianson, D W]]
	[[Category: Decroos, C]]		[[Category: Decroos, C]]
-	[[Category: ~~Christianson, D.W~~]]	+	[[Category: Acetylpolyamine amidohydrolase]]
		+	[[Category: Arginase fold]]
		+	[[Category: Enzyme-inhibitor complex]]
		+	[[Category: Hydrolase-hydrolase inhibitor complex]]
		+	[[Category: Polyamine]]

OCA at 11:13, 3 June 2015

OCA — Wed, 03 Jun 2015 11:13:17 GMT

←Older revision		Revision as of 11:13, 3 June 2015
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4zum is ON HOLD	+	The entry 4zum is ON HOLD until Paper Publication

	Authors: Decroos, C., Christianson, D.W.		Authors: Decroos, C., Christianson, D.W.

OCA: Protected "4zum" [edit=sysop:move=sysop]

OCA — Wed, 27 May 2015 14:44:16 GMT

Protected "4zum" [edit=sysop:move=sysop]

←Older revision

Revision as of 14:44, 27 May 2015

OCA: New page: '''Unreleased structure''' The entry 4zum is ON HOLD Authors: Decroos, C., Christianson, D.W. Description: Crystal structure of acetylpolyamine amidohydrolase from Mycoplana ramosa in ...

OCA — Wed, 27 May 2015 14:44:15 GMT

New page: '''Unreleased structure''' The entry 4zum is ON HOLD Authors: Decroos, C., Christianson, D.W. Description: Crystal structure of acetylpolyamine amidohydrolase from Mycoplana ramosa in ...

New page

'''Unreleased structure'''

The entry 4zum is ON HOLD

Authors: Decroos, C., Christianson, D.W.

Description: Crystal structure of acetylpolyamine amidohydrolase from Mycoplana ramosa in complex with a trifluoromethylketone inhibitor
[[Category: Unreleased Structures]]
[[Category: Decroos, C]]
[[Category: Christianson, D.W]]