4zx2 - Revision history

OCA at 08:25, 27 September 2023

OCA — Wed, 27 Sep 2023 08:25:43 GMT

←Older revision		Revision as of 08:25, 27 September 2023
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4zx2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZX2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ZX2 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4zx2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZX2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ZX2 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=4TE:(1S,2R,3S,4R,5S)-5-METHYL-7-OXABICYCLO[2.2.1]HEPTANE-2,3-DICARBOXYLIC+ACID'>4TE</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=MRD:(4R)-2-METHYLPENTANE-2,4-DIOL'>MRD</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.23Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=4TE:(1S,2R,3S,4R,5S)-5-METHYL-7-OXABICYCLO[2.2.1]HEPTANE-2,3-DICARBOXYLIC+ACID'>4TE</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=MRD:(4R)-2-METHYLPENTANE-2,4-DIOL'>MRD</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4zx2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zx2 OCA], [https://pdbe.org/4zx2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4zx2 RCSB], [https://www.ebi.ac.uk/pdbsum/4zx2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4zx2 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4zx2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zx2 OCA], [https://pdbe.org/4zx2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4zx2 RCSB], [https://www.ebi.ac.uk/pdbsum/4zx2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4zx2 ProSAT]</span></td></tr>
	</table>		</table>

OCA at 07:45, 18 May 2023

OCA — Thu, 18 May 2023 07:45:08 GMT

←Older revision		Revision as of 07:45, 18 May 2023
Line 3:		Line 3:
	<StructureSection load='4zx2' size='340' side='right'caption='[[4zx2]], [[Resolution\|resolution]] 1.23Å' scene=''>		<StructureSection load='4zx2' size='340' side='right'caption='[[4zx2]], [[Resolution\|resolution]] 1.23Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4zx2]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZX2 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4ZX2 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4zx2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZX2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ZX2 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=4TE:(1S,2R,3S,4R,5S)-5-METHYL-7-OXABICYCLO[2.2.1]HEPTANE-2,3-DICARBOXYLIC+ACID'>4TE</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=MRD:(4R)-2-METHYLPENTANE-2,4-DIOL'>MRD</scene~~></td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=4TE:(1S,2R,3S,4R,5S)-5-METHYL-7-OXABICYCLO[2.2.1]HEPTANE-2,3-DICARBOXYLIC+ACID'>4TE</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=MRD:(4R)-2-METHYLPENTANE-2,4-DIOL'>MRD</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1s95\|1s95]], [[4zvz\|4zvz]]</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4zx2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zx2 OCA], [https://pdbe.org/4zx2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4zx2 RCSB], [https://www.ebi.ac.uk/pdbsum/4zx2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4zx2 ProSAT]</span></td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PPP5C, PPP5 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>~~	+
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphoprotein_phosphatase Phosphoprotein phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.16 3.1.3.16] </span></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4zx2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zx2 OCA], [~~http~~://pdbe.org/4zx2 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4zx2 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4zx2 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4zx2 ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/PPP5_HUMAN PPP5_HUMAN]] May play a role in the regulation of RNA biogenesis and/or mitosis. In vitro, dephosphorylates serine residues of skeletal muscle phosphorylase and histone H1.	+	[https://www.uniprot.org/uniprot/PPP5_HUMAN PPP5_HUMAN] May play a role in the regulation of RNA biogenesis and/or mitosis. In vitro, dephosphorylates serine residues of skeletal muscle phosphorylase and histone H1.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 23:		Line 20:

	==See Also==		==See Also==
-	*[[Protein phosphatase\|Protein phosphatase]]	+	*[[Protein phosphatase 3D structures\|Protein phosphatase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	~~[[Category: Phosphoprotein phosphatase]]~~	+	[[Category: Chattopadhyay D]]
-	[[Category: Chattopadhyay, D]]	+	[[Category: Honkanen RE]]
-	[[Category: Honkanen~~, R E~~]]	+	[[Category: Salter EA]]
-	[[Category: Salter~~, E A~~]]	+	[[Category: Swingle MR]]
-	[[Category: Swingle~~, M R~~]]	+	[[Category: Wierzbicki A]]
-	[[Category: Wierzbicki, A]]	+
-	~~[[Category: Hydrolase-hydrolase inhibitor complex]]~~	+
-	~~[[Category: Protein phosphatase 5~~]]	+

OCA at 07:25, 27 November 2019

OCA — Wed, 27 Nov 2019 07:25:49 GMT

←Older revision		Revision as of 07:25, 27 November 2019
Line 1:		Line 1:

	==Co-crystal structures of PP5 in complex with 5-methyl-7-oxabicyclo[2.2.1]heptane-2,3-dicarboxylic acid==		==Co-crystal structures of PP5 in complex with 5-methyl-7-oxabicyclo[2.2.1]heptane-2,3-dicarboxylic acid==
-	<StructureSection load='4zx2' size='340' side='right' caption='[[4zx2]], [[Resolution\|resolution]] 1.23Å' scene=''>	+	<StructureSection load='4zx2' size='340' side='right'caption='[[4zx2]], [[Resolution\|resolution]] 1.23Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4zx2]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZX2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ZX2 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4zx2]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZX2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ZX2 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=4TE:(1S,2R,3S,4R,5S)-5-METHYL-7-OXABICYCLO[2.2.1]HEPTANE-2,3-DICARBOXYLIC+ACID'>4TE</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=MRD:(4R)-2-METHYLPENTANE-2,4-DIOL'>MRD</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=4TE:(1S,2R,3S,4R,5S)-5-METHYL-7-OXABICYCLO[2.2.1]HEPTANE-2,3-DICARBOXYLIC+ACID'>4TE</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=MRD:(4R)-2-METHYLPENTANE-2,4-DIOL'>MRD</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1s95\|1s95]], [[4zvz\|4zvz]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1s95\|1s95]], [[4zvz\|4zvz]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PPP5C, PPP5 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphoprotein_phosphatase Phosphoprotein phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.16 3.1.3.16] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphoprotein_phosphatase Phosphoprotein phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.16 3.1.3.16] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4zx2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zx2 OCA], [http://pdbe.org/4zx2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4zx2 RCSB], [http://www.ebi.ac.uk/pdbsum/4zx2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4zx2 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4zx2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zx2 OCA], [http://pdbe.org/4zx2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4zx2 RCSB], [http://www.ebi.ac.uk/pdbsum/4zx2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4zx2 ProSAT]</span></td></tr>
Line 22:		Line 23:

	==See Also==		==See Also==
-	*[[~~Serine/threonine protein~~ phosphatase\|~~Serine/threonine protein~~ phosphatase]]	+	*[[Protein phosphatase\|Protein phosphatase]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Phosphoprotein phosphatase]]		[[Category: Phosphoprotein phosphatase]]
	[[Category: Chattopadhyay, D]]		[[Category: Chattopadhyay, D]]

OCA at 06:46, 9 March 2017

OCA — Thu, 09 Mar 2017 06:46:09 GMT

←Older revision		Revision as of 06:46, 9 March 2017
Line 7:		Line 7:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1s95\|1s95]], [[4zvz\|4zvz]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1s95\|1s95]], [[4zvz\|4zvz]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphoprotein_phosphatase Phosphoprotein phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.16 3.1.3.16] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphoprotein_phosphatase Phosphoprotein phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.16 3.1.3.16] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4zx2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zx2 OCA], [http://pdbe.org/4zx2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4zx2 RCSB], [http://www.ebi.ac.uk/pdbsum/4zx2 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4zx2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zx2 OCA], [http://pdbe.org/4zx2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4zx2 RCSB], [http://www.ebi.ac.uk/pdbsum/4zx2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4zx2 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 13:		Line 13:
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
-	~~Serine~~/~~threonine~~ protein ~~phosphatase-5 (PP5) affects many signaling networks that regulate cell growth~~ and ~~cellular responses~~ to ~~stress~~. ~~Here~~ we ~~report~~ the crystal ~~structure~~ of ~~the PP5 catalytic domain (PP5c) at a resolution~~ of 1.~~6 A. From this structure we propose~~ a ~~mechanism for PP5~~-~~mediated hydrolysis of phosphoprotein substrates~~, which ~~requires~~ the ~~precise positioning~~ of ~~two~~ metal ions ~~within a conserved Asp271-M1:M2-W1~~-~~His427-His304-Asp274 catalytic motif~~ (~~where M1~~ and ~~M2 are metals and W1 is a water molecule~~). ~~The structure of PP5c provides a~~ structural basis for ~~explaining~~ the ~~exceptional catalytic proficiency~~ of ~~protein phosphatases, which are among the most powerful known catalysts~~. ~~Resolution of the entire C terminus revealed a~~ novel ~~subdomain, and the structure of the PP5c should~~ also ~~aid development~~ of ~~type~~-~~specific inhibitors~~.	+	Cantharidin is a natural toxin and an active constituent in a traditional Chinese medicine used to treat tumors. Cantharidin acts as a semi-selective inhibitor of PPP-family ser/thr protein phosphatases. Despite sharing a common catalytic mechanism and marked structural similarity with PP1C, PP2AC and PP5C, human PP4C was found to be insensitive to the inhibitory activity of cantharidin. To explore the molecular basis for this selectivity, we synthesized and tested novel C5/C6-derivatives designed from quantum-based modeling of the interactions revealed in the co-crystal structures of PP5C in complex with cantharidin. Structure-activity relationship studies and analysis of high-resolution (1.25A) PP5C-inhibitor co-crystal structures reveal close contacts between the inhibitor bridgehead oxygen and both a catalytic metal ion and a non-catalytic phenylalanine residue, the latter of which is substituted by tryptophan in PP4C. Quantum chemistry calculations predicted that steric clashes with the bulkier tryptophan side chain in PP4C would force all cantharidin-based inhibitors into an unfavorable binding mode, disrupting the strong coordination of active site metal ions observed in the PP5C co-crystal structures, thereby rendering PP4C insensitive to the inhibitors. This prediction was confirmed by inhibition studies employing native human PP4C. Mutation of PP5C (F446W) and PP1C (F257W), to mimic the PP4C active site, resulted in markedly suppressed sensitivity to cantharidin. These observations provide insight into the structural basis for the natural selectivity of cantharidin and provide an avenue for PP4C deselection. The novel crystal structures also provide insight into interactions that provide increased selectivity of the C5/C6 modifications for PP5C versus other PPP-family phosphatases.

-	~~Structural basis for the catalytic activity~~ of ~~human serine~~/~~threonine~~ protein ~~phosphatase~~-5.,Swingle MR, Honkanen RE~~, Ciszak EM J Biol Chem~~. ~~2004 Aug 6~~;~~279(32)~~:~~33992~~-9. Epub ~~2004 May~~ 23. PMID:~~15155720~~<ref>PMID:~~15155720~~</ref>	+	Crystal structures and mutagenesis of PPP-family ser/thr protein phosphatases elucidate the selectivity of cantharidin and novel norcantharidin-based inhibitors of PP5C.,Chattopadhyay D, Swingle MR, Salter EA, Wood E, D'Arcy B, Zivanov C, Abney K, Musiyenko A, Rusin SF, Kettenbach A, Yet L, Schroeder CE, Golden JE, Dunham WH, Gingras AC, Banerjee S, Forbes D, Wierzbicki A, Honkanen RE Biochem Pharmacol. 2016 Jun 1;109:14-26. doi: 10.1016/j.bcp.2016.03.011. Epub, 2016 Mar 23. PMID:27002182<ref>PMID:27002182</ref>

	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
	<div class="pdbe-citations 4zx2" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 4zx2" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Serine/threonine protein phosphatase\|Serine/threonine protein phosphatase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 11:58, 13 May 2016

OCA — Fri, 13 May 2016 11:58:59 GMT

←Older revision		Revision as of 11:58, 13 May 2016
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 4zx2 is ~~ON HOLD until Paper~~ Publication	+	==Co-crystal structures of PP5 in complex with 5-methyl-7-oxabicyclo[2.2.1]heptane-2,3-dicarboxylic acid==
		+	<StructureSection load='4zx2' size='340' side='right' caption='[[4zx2]], [[Resolution\|resolution]] 1.23Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4zx2]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZX2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ZX2 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=4TE:(1S,2R,3S,4R,5S)-5-METHYL-7-OXABICYCLO[2.2.1]HEPTANE-2,3-DICARBOXYLIC+ACID'>4TE</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=MRD:(4R)-2-METHYLPENTANE-2,4-DIOL'>MRD</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1s95\|1s95]], [[4zvz\|4zvz]]</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphoprotein_phosphatase Phosphoprotein phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.16 3.1.3.16] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4zx2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zx2 OCA], [http://pdbe.org/4zx2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4zx2 RCSB], [http://www.ebi.ac.uk/pdbsum/4zx2 PDBsum]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/PPP5_HUMAN PPP5_HUMAN]] May play a role in the regulation of RNA biogenesis and/or mitosis. In vitro, dephosphorylates serine residues of skeletal muscle phosphorylase and histone H1.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Serine/threonine protein phosphatase-5 (PP5) affects many signaling networks that regulate cell growth and cellular responses to stress. Here we report the crystal structure of the PP5 catalytic domain (PP5c) at a resolution of 1.6 A. From this structure we propose a mechanism for PP5-mediated hydrolysis of phosphoprotein substrates, which requires the precise positioning of two metal ions within a conserved Asp271-M1:M2-W1-His427-His304-Asp274 catalytic motif (where M1 and M2 are metals and W1 is a water molecule). The structure of PP5c provides a structural basis for explaining the exceptional catalytic proficiency of protein phosphatases, which are among the most powerful known catalysts. Resolution of the entire C terminus revealed a novel subdomain, and the structure of the PP5c should also aid development of type-specific inhibitors.

-	~~Authors: Chattopadhyay, D~~., Swingle, ~~M.R.~~, ~~Salter, E~~.A.~~, Wierzbicki, A., Honkanen, R.E~~.	+	Structural basis for the catalytic activity of human serine/threonine protein phosphatase-5.,Swingle MR, Honkanen RE, Ciszak EM J Biol Chem. 2004 Aug 6;279(32):33992-9. Epub 2004 May 23. PMID:15155720<ref>PMID:15155720</ref>

-	~~Description: Co-crystal structures~~ of ~~PP5 in complex with 5-methyl-7-oxabicyclo[2~~.2.~~1]heptane~~-~~2,3~~-~~dicarboxylic acid~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category~~: ~~Unreleased Structures]]~~	+	</div>
-	~~[[Category: Swingle, M.R]]~~	+	<div class="pdbe-citations 4zx2" style="background-color:#fffaf0;"></div>
-	~~[[Category: Wierzbicki, A]]~~	+	== References ==
-	~~[[Category: Honkanen, R.E]]~~	+	<references/>
-	[[Category: ~~Salter, E.A~~]]	+	__TOC__
		+	</StructureSection>
		+	[[Category: Phosphoprotein phosphatase]]
	[[Category: Chattopadhyay, D]]		[[Category: Chattopadhyay, D]]
		+	[[Category: Honkanen, R E]]
		+	[[Category: Salter, E A]]
		+	[[Category: Swingle, M R]]
		+	[[Category: Wierzbicki, A]]
		+	[[Category: Hydrolase-hydrolase inhibitor complex]]
		+	[[Category: Protein phosphatase 5]]

OCA at 00:14, 1 December 2015

OCA — Tue, 01 Dec 2015 00:14:22 GMT

←Older revision		Revision as of 00:14, 1 December 2015
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4zx2 is ON HOLD	+	The entry 4zx2 is ON HOLD until Paper Publication

	Authors: Chattopadhyay, D., Swingle, M.R., Salter, E.A., Wierzbicki, A., Honkanen, R.E.		Authors: Chattopadhyay, D., Swingle, M.R., Salter, E.A., Wierzbicki, A., Honkanen, R.E.
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	Description: Co-crystal structures of PP5 in complex with 5-methyl-7-oxabicyclo[2.2.1]heptane-2,3-dicarboxylic acid		Description: Co-crystal structures of PP5 in complex with 5-methyl-7-oxabicyclo[2.2.1]heptane-2,3-dicarboxylic acid
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
		+	[[Category: Swingle, M.R]]
	[[Category: Wierzbicki, A]]		[[Category: Wierzbicki, A]]
	[[Category: Honkanen, R.E]]		[[Category: Honkanen, R.E]]
-	[[Category: Chattopadhyay, D]]
-	[[Category: Swingle, M.R]]
	[[Category: Salter, E.A]]		[[Category: Salter, E.A]]
		+	[[Category: Chattopadhyay, D]]

OCA: Protected "4zx2" [edit=sysop:move=sysop]

OCA — Wed, 27 May 2015 14:51:06 GMT

Protected "4zx2" [edit=sysop:move=sysop]

←Older revision

Revision as of 14:51, 27 May 2015

OCA: New page: '''Unreleased structure''' The entry 4zx2 is ON HOLD Authors: Chattopadhyay, D., Swingle, M.R., Salter, E.A., Wierzbicki, A., Honkanen, R.E. Description: Co-crystal structures of PP5 i...

OCA — Wed, 27 May 2015 14:51:05 GMT

New page: '''Unreleased structure''' The entry 4zx2 is ON HOLD Authors: Chattopadhyay, D., Swingle, M.R., Salter, E.A., Wierzbicki, A., Honkanen, R.E. Description: Co-crystal structures of PP5 i...

New page

'''Unreleased structure'''

The entry 4zx2 is ON HOLD

Authors: Chattopadhyay, D., Swingle, M.R., Salter, E.A., Wierzbicki, A., Honkanen, R.E.

Description: Co-crystal structures of PP5 in complex with 5-methyl-7-oxabicyclo[2.2.1]heptane-2,3-dicarboxylic acid
[[Category: Unreleased Structures]]
[[Category: Wierzbicki, A]]
[[Category: Honkanen, R.E]]
[[Category: Chattopadhyay, D]]
[[Category: Swingle, M.R]]
[[Category: Salter, E.A]]