4zxu - Revision history

OCA at 08:25, 27 September 2023

OCA — Wed, 27 Sep 2023 08:25:56 GMT

←Older revision		Revision as of 08:25, 27 September 2023
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4zxu]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus_COL Staphylococcus aureus subsp. aureus COL]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZXU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ZXU FirstGlance]. <br>		<table><tr><td colspan='2'>[[4zxu]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus_COL Staphylococcus aureus subsp. aureus COL]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZXU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ZXU FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.85Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4zxu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zxu OCA], [https://pdbe.org/4zxu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4zxu RCSB], [https://www.ebi.ac.uk/pdbsum/4zxu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4zxu ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4zxu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zxu OCA], [https://pdbe.org/4zxu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4zxu RCSB], [https://www.ebi.ac.uk/pdbsum/4zxu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4zxu ProSAT]</span></td></tr>
	</table>		</table>

OCA at 07:46, 18 May 2023

OCA — Thu, 18 May 2023 07:46:02 GMT

←Older revision		Revision as of 07:46, 18 May 2023
Line 3:		Line 3:
	<StructureSection load='4zxu' size='340' side='right'caption='[[4zxu]], [[Resolution\|resolution]] 2.85Å' scene=''>		<StructureSection load='4zxu' size='340' side='right'caption='[[4zxu]], [[Resolution\|resolution]] 2.85Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4zxu]] is a 8 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Staac Staac~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZXU OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4ZXU FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4zxu]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus_COL Staphylococcus aureus subsp. aureus COL]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZXU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ZXU FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene~~></td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4mpb\|4mpb]], [[4mpy\|4mpy]], [[4nea\|4nea]], [[4nu9\|4nu9]], [[4qto\|4qto]], [[4qn2\|4qn2]], [[4q92\|4q92]], [[4qje\|4qje]], [[4zwl\|4zwl]]</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4zxu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zxu OCA], [https://pdbe.org/4zxu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4zxu RCSB], [https://www.ebi.ac.uk/pdbsum/4zxu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4zxu ProSAT]</span></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">betB, SACOL2628 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=93062 STAAC])</td></tr>	+
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Betaine-aldehyde_dehydrogenase Betaine-aldehyde dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.2.1.8 1.2.1.8] </span></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4zxu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zxu OCA], [~~http~~://pdbe.org/4zxu PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4zxu RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4zxu PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4zxu ProSAT]</span></td></tr>	+
	</table>		</table>
-	~~<div style~~=~~"background-color:#fffaf0;">~~	+	== Function ==
-	=~~= Publication Abstract from PubMed~~ ==	+	[https://www.uniprot.org/uniprot/A0A0M3KL41_STAAC A0A0M3KL41_STAAC]
-	When exposed to high osmolarity, methicillin-resistant Staphylococcus aureus (MRSA) restores its growth and establishes a new steady state by accumulating the osmoprotectant metabolite betaine. Effective osmoregulation has also been implicated in the acquirement of a profound antibiotic resistance by MRSA. Betaine can be obtained from the bacterial habitat or produced intracellularly from choline via the toxic betaine aldehyde (BA) employing the choline dehydrogenase and betaine aldehyde dehydrogenase (BADH) enzymes. Here, it is shown that the putative betaine aldehyde dehydrogenase SACOL2628 from the early MRSA isolate COL (SaBADH) utilizes betaine aldehyde as the primary substrate and nicotinamide adenine dinucleotide (NAD(+)) as the cofactor. Surface plasmon resonance experiments revealed that the affinity of NAD(+), NADH and BA for SaBADH is affected by temperature, pH and buffer composition. Five crystal structures of the wild type and three structures of the Gly234Ser mutant of SaBADH in the apo and holo forms provide details of the molecular mechanisms of activity and substrate specificity/inhibition of this enzyme.	+
-		+
-	Structural and functional analysis of betaine aldehyde dehydrogenase from Staphylococcus aureus.,Halavaty AS, Rich RL, Chen C, Joo JC, Minasov G, Dubrovska I, Winsor JR, Myszka DG, Duban M, Shuvalova L, Yakunin AF, Anderson WF Acta Crystallogr D Biol Crystallogr. 2015 May;71(Pt 5):~~1159-75. doi:, 10.1107~~/~~S1399004715004228. Epub 2015 Apr 25. PMID:25945581<ref>PMID:25945581<~~/~~ref>~~	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U~~.S. ~~National Library of Medicine.<br>~~	+
-	</~~div>~~	+
-	~~<div class="pdbe-citations 4zxu" style="background-color:#fffaf0;"><~~/~~div>~~	+

	==See Also==		==See Also==
	*[[Aldehyde dehydrogenase 3D structures\|Aldehyde dehydrogenase 3D structures]]		*[[Aldehyde dehydrogenase 3D structures\|Aldehyde dehydrogenase 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Betaine-aldehyde dehydrogenase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Staac~~]]	+	[[Category: Staphylococcus aureus subsp. aureus COL]]
-	[[Category: Anderson~~, W F]]~~	+	[[Category: Anderson WF]]
-	~~[[Category: Structural genomic~~]]	+	[[Category: Chen C]]
-	[[Category: Chen, C]]	+	[[Category: Halavaty AS]]
-	[[Category: Halavaty~~, A S~~]]	+	[[Category: Joo JC]]
-	[[Category: Joo~~, J C~~]]	+	[[Category: Minasov G]]
-	[[Category: Minasov, G]]	+	[[Category: Yakunin AF]]
-	[[Category: Yakunin~~, A F]]~~	+
-	~~[[Category: Betaine aldehyde dehydrogenase]]~~	+
-	~~[[Category: Csgid]]~~	+
-	~~[[Category: Nad]]~~	+
-	~~[[Category: National institute of allergy and infectious disease]]~~	+
-	~~[[Category: Niaid]]~~	+
-	~~[[Category: Oxidoreductase]]~~	+
-	~~[[Category: Rossmann fold~~]]	+

OCA at 07:56, 26 June 2019

OCA — Wed, 26 Jun 2019 07:56:00 GMT

←Older revision		Revision as of 07:56, 26 June 2019
Line 1:		Line 1:

	==2.85 Angstrom resolution crystal structure of betaine aldehyde dehydrogenase (betB) H448F/P449M double mutant from Staphylococcus aureus in complex with NAD+ and BME-free Cys289==		==2.85 Angstrom resolution crystal structure of betaine aldehyde dehydrogenase (betB) H448F/P449M double mutant from Staphylococcus aureus in complex with NAD+ and BME-free Cys289==
-	<StructureSection load='4zxu' size='340' side='right' caption='[[4zxu]], [[Resolution\|resolution]] 2.85Å' scene=''>	+	<StructureSection load='4zxu' size='340' side='right'caption='[[4zxu]], [[Resolution\|resolution]] 2.85Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4zxu]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZXU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ZXU FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4zxu]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Staac Staac]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZXU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ZXU FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4mpb\|4mpb]], [[4mpy\|4mpy]], [[4nea\|4nea]], [[4nu9\|4nu9]], [[4qto\|4qto]], [[4qn2\|4qn2]], [[4q92\|4q92]], [[4qje\|4qje]], [[4zwl\|4zwl]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4mpb\|4mpb]], [[4mpy\|4mpy]], [[4nea\|4nea]], [[4nu9\|4nu9]], [[4qto\|4qto]], [[4qn2\|4qn2]], [[4q92\|4q92]], [[4qje\|4qje]], [[4zwl\|4zwl]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">betB, SACOL2628 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=93062 STAAC])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Betaine-aldehyde_dehydrogenase Betaine-aldehyde dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.2.1.8 1.2.1.8] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Betaine-aldehyde_dehydrogenase Betaine-aldehyde dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.2.1.8 1.2.1.8] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4zxu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zxu OCA], [http://pdbe.org/4zxu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4zxu RCSB], [http://www.ebi.ac.uk/pdbsum/4zxu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4zxu ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4zxu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zxu OCA], [http://pdbe.org/4zxu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4zxu RCSB], [http://www.ebi.ac.uk/pdbsum/4zxu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4zxu ProSAT]</span></td></tr>
Line 20:		Line 21:

	==See Also==		==See Also==
-	*[[Aldehyde dehydrogenase\|Aldehyde dehydrogenase]]	+	*[[Aldehyde dehydrogenase 3D structures\|Aldehyde dehydrogenase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 26:		Line 27:
	</StructureSection>		</StructureSection>
	[[Category: Betaine-aldehyde dehydrogenase]]		[[Category: Betaine-aldehyde dehydrogenase]]
		+	[[Category: Large Structures]]
		+	[[Category: Staac]]
	[[Category: Anderson, W F]]		[[Category: Anderson, W F]]
	[[Category: Structural genomic]]		[[Category: Structural genomic]]

OCA at 17:03, 22 March 2017

OCA — Wed, 22 Mar 2017 17:03:27 GMT

←Older revision		Revision as of 17:03, 22 March 2017
Line 1:		Line 1:
		+
	==2.85 Angstrom resolution crystal structure of betaine aldehyde dehydrogenase (betB) H448F/P449M double mutant from Staphylococcus aureus in complex with NAD+ and BME-free Cys289==		==2.85 Angstrom resolution crystal structure of betaine aldehyde dehydrogenase (betB) H448F/P449M double mutant from Staphylococcus aureus in complex with NAD+ and BME-free Cys289==
	<StructureSection load='4zxu' size='340' side='right' caption='[[4zxu]], [[Resolution\|resolution]] 2.85Å' scene=''>		<StructureSection load='4zxu' size='340' side='right' caption='[[4zxu]], [[Resolution\|resolution]] 2.85Å' scene=''>
Line 6:		Line 7:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4mpb\|4mpb]], [[4mpy\|4mpy]], [[4nea\|4nea]], [[4nu9\|4nu9]], [[4qto\|4qto]], [[4qn2\|4qn2]], [[4q92\|4q92]], [[4qje\|4qje]], [[4zwl\|4zwl]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4mpb\|4mpb]], [[4mpy\|4mpy]], [[4nea\|4nea]], [[4nu9\|4nu9]], [[4qto\|4qto]], [[4qn2\|4qn2]], [[4q92\|4q92]], [[4qje\|4qje]], [[4zwl\|4zwl]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Betaine-aldehyde_dehydrogenase Betaine-aldehyde dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.2.1.8 1.2.1.8] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Betaine-aldehyde_dehydrogenase Betaine-aldehyde dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.2.1.8 1.2.1.8] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4zxu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zxu OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4zxu RCSB], [http://www.ebi.ac.uk/pdbsum/4zxu PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4zxu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zxu OCA], [http://pdbe.org/4zxu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4zxu RCSB], [http://www.ebi.ac.uk/pdbsum/4zxu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4zxu ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 16:		Line 17:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4zxu" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Aldehyde dehydrogenase\|Aldehyde dehydrogenase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 14:54, 17 June 2015

OCA — Wed, 17 Jun 2015 14:54:34 GMT

←Older revision		Revision as of 14:54, 17 June 2015
Line 1:		Line 1:
-	'''~~Unreleased~~ structure'''	+	==2.85 Angstrom resolution crystal structure of betaine aldehyde dehydrogenase (betB) H448F/P449M double mutant from Staphylococcus aureus in complex with NAD+ and BME-free Cys289==
		+	<StructureSection load='4zxu' size='340' side='right' caption='[[4zxu]], [[Resolution\|resolution]] 2.85Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4zxu]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZXU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ZXU FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4mpb\|4mpb]], [[4mpy\|4mpy]], [[4nea\|4nea]], [[4nu9\|4nu9]], [[4qto\|4qto]], [[4qn2\|4qn2]], [[4q92\|4q92]], [[4qje\|4qje]], [[4zwl\|4zwl]]</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Betaine-aldehyde_dehydrogenase Betaine-aldehyde dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.2.1.8 1.2.1.8] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4zxu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zxu OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4zxu RCSB], [http://www.ebi.ac.uk/pdbsum/4zxu PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	When exposed to high osmolarity, methicillin-resistant Staphylococcus aureus (MRSA) restores its growth and establishes a new steady state by accumulating the osmoprotectant metabolite betaine. Effective osmoregulation has also been implicated in the acquirement of a profound antibiotic resistance by MRSA. Betaine can be obtained from the bacterial habitat or produced intracellularly from choline via the toxic betaine aldehyde (BA) employing the choline dehydrogenase and betaine aldehyde dehydrogenase (BADH) enzymes. Here, it is shown that the putative betaine aldehyde dehydrogenase SACOL2628 from the early MRSA isolate COL (SaBADH) utilizes betaine aldehyde as the primary substrate and nicotinamide adenine dinucleotide (NAD(+)) as the cofactor. Surface plasmon resonance experiments revealed that the affinity of NAD(+), NADH and BA for SaBADH is affected by temperature, pH and buffer composition. Five crystal structures of the wild type and three structures of the Gly234Ser mutant of SaBADH in the apo and holo forms provide details of the molecular mechanisms of activity and substrate specificity/inhibition of this enzyme.

-	~~The entry 4zxu is ON HOLD~~	+	Structural and functional analysis of betaine aldehyde dehydrogenase from Staphylococcus aureus.,Halavaty AS, Rich RL, Chen C, Joo JC, Minasov G, Dubrovska I, Winsor JR, Myszka DG, Duban M, Shuvalova L, Yakunin AF, Anderson WF Acta Crystallogr D Biol Crystallogr. 2015 May;71(Pt 5):1159-75. doi:, 10.1107/S1399004715004228. Epub 2015 Apr 25. PMID:25945581<ref>PMID:25945581</ref>

-	~~Authors: Halavaty~~, A.S.~~, Minasov, G., Chen, C., Joo, J.C., Yakunin, A.F., Anderson, W.F., Center for Structural Genomics~~ of ~~Infectious Diseases (CSGID)~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~Description~~: ~~2.85 Angstrom resolution crystal structure of betaine~~ aldehyde dehydrogenase ~~(betB) H448F/P449M double mutant from Staphylococcus aureus in complex with NAD+ and BME-free Cys289~~	+	== References ==
-	[[Category: ~~Unreleased Structures~~]]	+	<references/>
-	[[Category: ~~Joo~~, J.C]]	+	__TOC__
-	[[Category: ~~Yakunin~~, A.F]]	+	</StructureSection>
		+	[[Category: Betaine-aldehyde dehydrogenase]]
		+	[[Category: Anderson, W F]]
		+	[[Category: Structural genomic]]
		+	[[Category: Chen, C]]
		+	[[Category: Halavaty, A S]]
		+	[[Category: Joo, J C]]
	[[Category: Minasov, G]]		[[Category: Minasov, G]]
-	[[Category: ~~Anderson~~, W.F]]	+	[[Category: Yakunin, A F]]
-	[[Category: ~~Center For Structural Genomics Of Infectious Diseases (~~Csgid)]]	+	[[Category: Betaine aldehyde dehydrogenase]]
-	[[Category: ~~Halavaty, A.S~~]]	+	[[Category: Csgid]]
-	[[Category: ~~Chen, C~~]]	+	[[Category: Nad]]
		+	[[Category: National institute of allergy and infectious disease]]
		+	[[Category: Niaid]]
		+	[[Category: Oxidoreductase]]
		+	[[Category: Rossmann fold]]

OCA: Protected "4zxu" [edit=sysop:move=sysop]

OCA — Wed, 27 May 2015 14:53:09 GMT

Protected "4zxu" [edit=sysop:move=sysop]

←Older revision

Revision as of 14:53, 27 May 2015

OCA: New page: '''Unreleased structure''' The entry 4zxu is ON HOLD Authors: Halavaty, A.S., Minasov, G., Chen, C., Joo, J.C., Yakunin, A.F., Anderson, W.F., Center for Structural Genomics of Infectio...

OCA — Wed, 27 May 2015 14:53:08 GMT

New page: '''Unreleased structure''' The entry 4zxu is ON HOLD Authors: Halavaty, A.S., Minasov, G., Chen, C., Joo, J.C., Yakunin, A.F., Anderson, W.F., Center for Structural Genomics of Infectio...

New page

'''Unreleased structure'''

The entry 4zxu is ON HOLD

Authors: Halavaty, A.S., Minasov, G., Chen, C., Joo, J.C., Yakunin, A.F., Anderson, W.F., Center for Structural Genomics of Infectious Diseases (CSGID)

Description: 2.85 Angstrom resolution crystal structure of betaine aldehyde dehydrogenase (betB) H448F/P449M double mutant from Staphylococcus aureus in complex with NAD+ and BME-free Cys289
[[Category: Unreleased Structures]]
[[Category: Joo, J.C]]
[[Category: Yakunin, A.F]]
[[Category: Minasov, G]]
[[Category: Anderson, W.F]]
[[Category: Center For Structural Genomics Of Infectious Diseases (Csgid)]]
[[Category: Halavaty, A.S]]
[[Category: Chen, C]]