4zyg - Revision history

OCA at 11:01, 10 January 2024

OCA — Wed, 10 Jan 2024 11:01:34 GMT

←Older revision		Revision as of 11:01, 10 January 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4zyg]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharolobus_solfataricus Saccharolobus solfataricus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZYG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ZYG FirstGlance]. <br>		<table><tr><td colspan='2'>[[4zyg]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharolobus_solfataricus Saccharolobus solfataricus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZYG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ZYG FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SMC:S-METHYLCYSTEINE'>SMC</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.8Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SMC:S-METHYLCYSTEINE'>SMC</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4zyg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zyg OCA], [https://pdbe.org/4zyg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4zyg RCSB], [https://www.ebi.ac.uk/pdbsum/4zyg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4zyg ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4zyg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zyg OCA], [https://pdbe.org/4zyg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4zyg RCSB], [https://www.ebi.ac.uk/pdbsum/4zyg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4zyg ProSAT]</span></td></tr>
	</table>		</table>

OCA at 07:46, 18 May 2023

OCA — Thu, 18 May 2023 07:46:44 GMT

←Older revision		Revision as of 07:46, 18 May 2023
Line 3:		Line 3:
	<StructureSection load='4zyg' size='340' side='right'caption='[[4zyg]], [[Resolution\|resolution]] 2.80Å' scene=''>		<StructureSection load='4zyg' size='340' side='right'caption='[[4zyg]], [[Resolution\|resolution]] 2.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4zyg]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Atcc_35091 Atcc 35091~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZYG OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4ZYG FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4zyg]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharolobus_solfataricus Saccharolobus solfataricus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZYG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ZYG FirstGlance]. <br>
-	</td></tr><tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=SMC:S-METHYLCYSTEINE'>SMC</scene~~></td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SMC:S-METHYLCYSTEINE'>SMC</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ogt, SSO2487 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2287 ATCC 35091])</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4zyg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zyg OCA], [https://pdbe.org/4zyg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4zyg RCSB], [https://www.ebi.ac.uk/pdbsum/4zyg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4zyg ProSAT]</span></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Methylated-DNA--[protein]-cysteine_S-methyltransferase Methylated-DNA--[protein]-cysteine S-methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.63 2.1.1.63] </span></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4zyg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zyg OCA], [~~http~~://pdbe.org/4zyg PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4zyg RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4zyg PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4zyg ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/~~OGT_SULSO OGT_SULSO]~~] Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs ~~alkylated guanine~~ in DNA by stoichiometrically transferring the ~~alkyl~~ group ~~at the O-6 position~~ to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.	+	[https://www.uniprot.org/uniprot/OGT_SACS2 OGT_SACS2] Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) and O4-methylthymine (O4-MeT) in DNA. Repairs the methylated nucleobase in DNA by stoichiometrically transferring the methyl group to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.[HAMAP-Rule:MF_00772]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 27:		Line 25:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Atcc 35091]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Miggiano, R~~]]	+	[[Category: Saccharolobus solfataricus]]
-	[[Category: ~~Rizzi, M~~]]	+	[[Category: Miggiano R]]
-	[[Category: ~~Rossi, F~~]]	+	[[Category: Rizzi M]]
-	[[Category: ~~Alkylated dna-protein alkyltransferase]]~~	+	[[Category: Rossi F]]
-	~~[[Category: Dna repair]]~~	+
-	~~[[Category: Extremophile]]~~	+
-	~~[[Category: Methylated protein]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 08:42, 26 June 2019

OCA — Wed, 26 Jun 2019 08:42:49 GMT

←Older revision		Revision as of 08:42, 26 June 2019
Line 1:		Line 1:
		+
	==Crystal structure of methylated Sulfolobus solfataricus O6-methylguanine methyltransferase==		==Crystal structure of methylated Sulfolobus solfataricus O6-methylguanine methyltransferase==
-	<StructureSection load='4zyg' size='340' side='right' caption='[[4zyg]], [[Resolution\|resolution]] 2.80Å' scene=''>	+	<StructureSection load='4zyg' size='340' side='right'caption='[[4zyg]], [[Resolution\|resolution]] 2.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4zyg]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZYG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ZYG FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4zyg]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_35091 Atcc 35091]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZYG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ZYG FirstGlance]. <br>
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=SMC:S-METHYLCYSTEINE'>SMC</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=SMC:S-METHYLCYSTEINE'>SMC</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ogt, SSO2487 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2287 ATCC 35091])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Methylated-DNA--[protein]-cysteine_S-methyltransferase Methylated-DNA--[protein]-cysteine S-methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.63 2.1.1.63] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Methylated-DNA--[protein]-cysteine_S-methyltransferase Methylated-DNA--[protein]-cysteine S-methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.63 2.1.1.63] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4zyg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zyg OCA], [http://pdbe.org/4zyg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4zyg RCSB], [http://www.ebi.ac.uk/pdbsum/4zyg PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4zyg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zyg OCA], [http://pdbe.org/4zyg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4zyg RCSB], [http://www.ebi.ac.uk/pdbsum/4zyg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4zyg ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 18:		Line 20:
	</div>		</div>
	<div class="pdbe-citations 4zyg" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 4zyg" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[DNA methyltransferase 3D structures\|DNA methyltransferase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Atcc 35091]]
		+	[[Category: Large Structures]]
	[[Category: Miggiano, R]]		[[Category: Miggiano, R]]
	[[Category: Rizzi, M]]		[[Category: Rizzi, M]]

OCA at 21:23, 30 November 2015

OCA — Mon, 30 Nov 2015 21:23:17 GMT

←Older revision		Revision as of 21:23, 30 November 2015
Line 5:		Line 5:
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=SMC:S-METHYLCYSTEINE'>SMC</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=SMC:S-METHYLCYSTEINE'>SMC</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Methylated-DNA--[protein]-cysteine_S-methyltransferase Methylated-DNA--[protein]-cysteine S-methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.63 2.1.1.63] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Methylated-DNA--[protein]-cysteine_S-methyltransferase Methylated-DNA--[protein]-cysteine S-methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.63 2.1.1.63] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4zyg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zyg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4zyg RCSB], [http://www.ebi.ac.uk/pdbsum/4zyg PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4zyg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zyg OCA], [http://pdbe.org/4zyg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4zyg RCSB], [http://www.ebi.ac.uk/pdbsum/4zyg PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 17:		Line 17:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4zyg" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 14:34, 12 August 2015

OCA — Wed, 12 Aug 2015 14:34:01 GMT

←Older revision		Revision as of 14:34, 12 August 2015
Line 1:		Line 1:
-	'''~~Unreleased~~ structure'''	+	==Crystal structure of methylated Sulfolobus solfataricus O6-methylguanine methyltransferase==
		+	<StructureSection load='4zyg' size='340' side='right' caption='[[4zyg]], [[Resolution\|resolution]] 2.80Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4zyg]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZYG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ZYG FirstGlance]. <br>
		+	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=SMC:S-METHYLCYSTEINE'>SMC</scene></td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Methylated-DNA--[protein]-cysteine_S-methyltransferase Methylated-DNA--[protein]-cysteine S-methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.63 2.1.1.63] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4zyg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zyg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4zyg RCSB], [http://www.ebi.ac.uk/pdbsum/4zyg PDBsum]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/OGT_SULSO OGT_SULSO]] Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Alkylated DNA-protein alkyltransferases repair alkylated DNA bases, which are among the most common DNA lesions, and are evolutionary conserved, from prokaryotes to higher eukaryotes. The human ortholog, hAGT, is involved in resistance to alkylating chemotherapy drugs. We report here on the alkylated DNA-protein alkyltransferase, SsOGT, from an archaeal species living at high temperature, a condition that enhances the harmful effect of DNA alkylation. The exceptionally high stability of SsOGT gave us the unique opportunity to perform structural and biochemical analysis of a protein of this class in its post-reaction form. This analysis, along with those performed on SsOGT in its ligand-free and DNA-bound forms, provides insights in the structure-function relationships of the protein before, during and after DNA repair, suggesting a molecular basis for DNA recognition, catalytic activity and protein post-reaction fate, and giving hints on the mechanism of alkylation-induced inactivation of this class of proteins.

-	~~The entry 4zyg is ON HOLD until Paper Publication~~	+	Structure-function relationships governing activity and stability of a DNA alkylation damage repair thermostable protein.,Perugino G, Miggiano R, Serpe M, Vettone A, Valenti A, Lahiri S, Rossi F, Rossi M, Rizzi M, Ciaramella M Nucleic Acids Res. 2015 Jul 30. pii: gkv774. PMID:26227971<ref>PMID:26227971</ref>

-	~~Authors: Miggiano~~, R.~~, Rossi, F~~.~~, Rizzi, M~~.	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~Description: Crystal structure of methylated Sulfolobus solfataricus O6-methylguanine methyltransferase~~	+	== References ==
-	~~[[Category: Unreleased Structures]]~~	+	<references/>
-	~~[[Category: Rossi, F]]~~	+	__TOC__
		+	</StructureSection>
	[[Category: Miggiano, R]]		[[Category: Miggiano, R]]
	[[Category: Rizzi, M]]		[[Category: Rizzi, M]]
		+	[[Category: Rossi, F]]
		+	[[Category: Alkylated dna-protein alkyltransferase]]
		+	[[Category: Dna repair]]
		+	[[Category: Extremophile]]
		+	[[Category: Methylated protein]]
		+	[[Category: Transferase]]

OCA at 11:19, 3 June 2015

OCA — Wed, 03 Jun 2015 11:19:42 GMT

←Older revision		Revision as of 11:19, 3 June 2015
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4zyg is ON HOLD	+	The entry 4zyg is ON HOLD until Paper Publication

	Authors: Miggiano, R., Rossi, F., Rizzi, M.		Authors: Miggiano, R., Rossi, F., Rizzi, M.

OCA: Protected "4zyg" [edit=sysop:move=sysop]

OCA — Wed, 27 May 2015 14:54:40 GMT

Protected "4zyg" [edit=sysop:move=sysop]

←Older revision

Revision as of 14:54, 27 May 2015

OCA: New page: '''Unreleased structure''' The entry 4zyg is ON HOLD Authors: Miggiano, R., Rossi, F., Rizzi, M. Description: Crystal structure of methylated Sulfolobus solfataricus O6-methylguanine m...

OCA — Wed, 27 May 2015 14:54:39 GMT

New page: '''Unreleased structure''' The entry 4zyg is ON HOLD Authors: Miggiano, R., Rossi, F., Rizzi, M. Description: Crystal structure of methylated Sulfolobus solfataricus O6-methylguanine m...

New page

'''Unreleased structure'''

The entry 4zyg is ON HOLD

Authors: Miggiano, R., Rossi, F., Rizzi, M.

Description: Crystal structure of methylated Sulfolobus solfataricus O6-methylguanine methyltransferase
[[Category: Unreleased Structures]]
[[Category: Rossi, F]]
[[Category: Miggiano, R]]
[[Category: Rizzi, M]]