5a07 - Revision history

OCA at 11:02, 10 January 2024

OCA — Wed, 10 Jan 2024 11:02:01 GMT

←Older revision		Revision as of 11:02, 10 January 2024
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5a07]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5A07 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5A07 FirstGlance]. <br>		<table><tr><td colspan='2'>[[5a07]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5A07 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5A07 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.9Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5a07 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5a07 OCA], [https://pdbe.org/5a07 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5a07 RCSB], [https://www.ebi.ac.uk/pdbsum/5a07 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5a07 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5a07 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5a07 OCA], [https://pdbe.org/5a07 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5a07 RCSB], [https://www.ebi.ac.uk/pdbsum/5a07 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5a07 ProSAT]</span></td></tr>
	</table>		</table>

OCA at 07:49, 18 May 2023

OCA — Thu, 18 May 2023 07:49:27 GMT

←Older revision		Revision as of 07:49, 18 May 2023
Line 3:		Line 3:
	<StructureSection load='5a07' size='340' side='right'caption='[[5a07]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='5a07' size='340' side='right'caption='[[5a07]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5a07]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Baker's_yeast Baker's yeast~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5A07 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5A07 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5a07]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5A07 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5A07 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5a08\|5a08]]~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5a07 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5a07 OCA], [https://pdbe.org/5a07 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5a07 RCSB], [https://www.ebi.ac.uk/pdbsum/5a07 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5a07 ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5a07 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5a07 OCA], [~~http~~://pdbe.org/5a07 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5a07 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5a07 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5a07 ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/KTR4_YEAST KTR4_YEAST]] Possible glycosyltransferase.	+	[https://www.uniprot.org/uniprot/KTR4_YEAST KTR4_YEAST] Possible glycosyltransferase.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 23:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Baker's yeast]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Guy, J E~~]]	+	[[Category: Saccharomyces cerevisiae S288C]]
-	[[Category: ~~Possner, D D.D~~]]	+	[[Category: Guy JE]]
-	[[Category: ~~Golgi apparatus]]~~	+	[[Category: Possner DDD]]
-	~~[[Category: Gt-a]]~~	+
-	~~[[Category: Mannosyltransferase]]~~	+
-	~~[[Category: Membrane protein]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 07:54, 26 June 2019

OCA — Wed, 26 Jun 2019 07:54:49 GMT

←Older revision		Revision as of 07:54, 26 June 2019
Line 1:		Line 1:
		+
	==X-ray structure of the mannosyltransferase Ktr4p from S. cerevisiae in complex with GDP==		==X-ray structure of the mannosyltransferase Ktr4p from S. cerevisiae in complex with GDP==
-	<StructureSection load='5a07' size='340' side='right' caption='[[5a07]], [[Resolution\|resolution]] 1.90Å' scene=''>	+	<StructureSection load='5a07' size='340' side='right'caption='[[5a07]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5a07]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5A07 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5A07 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5a07]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5A07 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5A07 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5a08\|5a08]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5a08\|5a08]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5a07 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5a07 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=5a07 RCSB], [http://www.ebi.ac.uk/pdbsum/5a07 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5a07 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5a07 OCA], [http://pdbe.org/5a07 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5a07 RCSB], [http://www.ebi.ac.uk/pdbsum/5a07 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5a07 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 17:		Line 18:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 5a07" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Baker's yeast]]
		+	[[Category: Large Structures]]
	[[Category: Guy, J E]]		[[Category: Guy, J E]]
	[[Category: Possner, D D.D]]		[[Category: Possner, D D.D]]

OCA at 06:29, 2 September 2015

OCA — Wed, 02 Sep 2015 06:29:07 GMT

←Older revision		Revision as of 06:29, 2 September 2015
Line 9:		Line 9:
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/KTR4_YEAST KTR4_YEAST]] Possible glycosyltransferase.		[[http://www.uniprot.org/uniprot/KTR4_YEAST KTR4_YEAST]] Possible glycosyltransferase.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	In the yeast Saccharomyces cerevisiae, members of the Kre2/Mnt1 protein family have been shown to be alpha-1,2-mannosyltransferases or alpha-1,2-mannosylphosphate transferases, utilising an Mn2+-coordinated GDP-mannose as the sugar donor and a variety of mannose derivatives as acceptors. Enzymes in this family are localised to the Golgi apparatus, and have been shown to be involved in both N- and O-linked glycosylation of newly-synthesised proteins, including cell wall glycoproteins. Our knowledge of the nine proteins in this family is however very incomplete at present. Only one family member, Kre2p/Mnt1p, has been studied by structural methods, and three (Ktr4p, Ktr5p, Ktr7p) are completely uncharacterised and remain classified only as putative glycosyltransferases. Here we use in vitro enzyme activity assays to provide experimental confirmation of the predicted glycosyltransferase activity of Ktr4p. Using GDP-mannose as the donor, we observe activity towards the acceptor methyl-alpha-mannoside, but little or no activity towards mannose or alpha-1,2-mannobiose. We also present the structure of the lumenal catalytic domain of S. cerevisiae Ktr4p, determined by X-ray crystallography to a resolution of 2.2 A, and the complex of the enzyme with GDP to 1.9 A resolution.
		+
		+	Structure of the Glycosyltransferase Ktr4p from Saccharomyces cerevisiae.,Possner DD, Claesson M, Guy JE PLoS One. 2015 Aug 21;10(8):e0136239. doi: 10.1371/journal.pone.0136239., eCollection 2015. PMID:26296208<ref>PMID:26296208</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 13:36, 26 August 2015

OCA — Wed, 26 Aug 2015 13:36:51 GMT

←Older revision		Revision as of 13:36, 26 August 2015
Line 1:		Line 1:
-	'''~~Unreleased structure~~'''	+	==X-ray structure of the mannosyltransferase Ktr4p from S. cerevisiae in complex with GDP==
-		+	<StructureSection load='5a07' size='340' side='right' caption='[[5a07]], [[Resolution\|resolution]] 1.90Å' scene=''>
-	~~The entry~~ 5a07 is ~~ON HOLD~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5a07]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5A07 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5A07 FirstGlance]. <br>
-	~~Authors~~: ~~Possner~~, D.D.D., ~~Guy~~, J.E.	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
-		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5a08\|5a08]]</td></tr>
-	~~Description~~: ~~X-ray structure of the mannosyltransferase Ktr4p from S~~. ~~cerevisiae in complex with GDP~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5a07 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5a07 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=5a07 RCSB], [http://www.ebi.ac.uk/pdbsum/5a07 PDBsum]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
-	[[Category: Possner, D.D.D]]	+	== Function ==
-	[[Category: ~~Guy, J.E~~]]	+	[[http://www.uniprot.org/uniprot/KTR4_YEAST KTR4_YEAST]] Possible glycosyltransferase.
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Guy, J E]]
		+	[[Category: Possner, D D.D]]
		+	[[Category: Golgi apparatus]]
		+	[[Category: Gt-a]]
		+	[[Category: Mannosyltransferase]]
		+	[[Category: Membrane protein]]
		+	[[Category: Transferase]]

OCA: Protected "5a07" [edit=sysop:move=sysop]

OCA — Thu, 30 Apr 2015 10:24:24 GMT

Protected "5a07" [edit=sysop:move=sysop]

←Older revision

Revision as of 10:24, 30 April 2015

OCA: New page: '''Unreleased structure''' The entry 5a07 is ON HOLD Authors: Possner, D.D.D., Guy, J.E. Description: X-ray structure of the mannosyltransferase Ktr4p from S. cerevisiae in complex wit...

OCA — Thu, 30 Apr 2015 10:24:22 GMT

New page: '''Unreleased structure''' The entry 5a07 is ON HOLD Authors: Possner, D.D.D., Guy, J.E. Description: X-ray structure of the mannosyltransferase Ktr4p from S. cerevisiae in complex wit...

New page

'''Unreleased structure'''

The entry 5a07 is ON HOLD

Authors: Possner, D.D.D., Guy, J.E.

Description: X-ray structure of the mannosyltransferase Ktr4p from S. cerevisiae in complex with GDP
[[Category: Unreleased Structures]]
[[Category: Possner, D.D.D]]
[[Category: Guy, J.E]]