5a5d - Revision history

OCA at 11:37, 9 May 2024

OCA — Thu, 09 May 2024 11:37:37 GMT

←Older revision		Revision as of 11:37, 9 May 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5a5d]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Campylobacter_jejuni Campylobacter jejuni]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5A5D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5A5D FirstGlance]. <br>		<table><tr><td colspan='2'>[[5a5d]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Campylobacter_jejuni Campylobacter jejuni]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5A5D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5A5D FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=5LC:N,N-PENTANE-1,5-DIYLBIS(2,3-DIHYDROXYBENZAMIDE)'>5LC</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.74Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=5LC:N,N-PENTANE-1,5-DIYLBIS(2,3-DIHYDROXYBENZAMIDE)'>5LC</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5a5d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5a5d OCA], [https://pdbe.org/5a5d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5a5d RCSB], [https://www.ebi.ac.uk/pdbsum/5a5d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5a5d ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5a5d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5a5d OCA], [https://pdbe.org/5a5d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5a5d RCSB], [https://www.ebi.ac.uk/pdbsum/5a5d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5a5d ProSAT]</span></td></tr>
	</table>		</table>

OCA at 04:39, 25 May 2023

OCA — Thu, 25 May 2023 04:39:57 GMT

←Older revision		Revision as of 04:39, 25 May 2023
Line 3:		Line 3:
	<StructureSection load='5a5d' size='340' side='right'caption='[[5a5d]], [[Resolution\|resolution]] 1.74Å' scene=''>		<StructureSection load='5a5d' size='340' side='right'caption='[[5a5d]], [[Resolution\|resolution]] 1.74Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5a5d]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"campylobacter_fetus_subsp._jejuni"_smibert_1974 "campylobacter fetus subsp.~~ jejuni~~" smibert 1974~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5A5D OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5A5D FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5a5d]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Campylobacter_jejuni Campylobacter jejuni]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5A5D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5A5D FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=5LC:N,N-PENTANE-1,5-DIYLBIS(2,3-DIHYDROXYBENZAMIDE)'>5LC</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=5LC:N,N-PENTANE-1,5-DIYLBIS(2,3-DIHYDROXYBENZAMIDE)'>5LC</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5a5d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5a5d OCA], [~~http~~://pdbe.org/5a5d PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5a5d RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5a5d PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5a5d ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5a5d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5a5d OCA], [https://pdbe.org/5a5d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5a5d RCSB], [https://www.ebi.ac.uk/pdbsum/5a5d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5a5d ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q0P8Q4_CAMJE Q0P8Q4_CAMJE]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 20:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Campylobacter ~~fetus subsp.~~ jejuni ~~smibert 1974~~]]	+	[[Category: Campylobacter jejuni]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Blagova, E]]	+	[[Category: Blagova E]]
-	[[Category: Duhme-Klair, A K]]	+	[[Category: Duhme-Klair A-K]]
-	[[Category: Hughes, A]]	+	[[Category: Hughes A]]
-	[[Category: Moroz~~, O V~~]]	+	[[Category: Moroz OV]]
-	[[Category: Raines~~, D J~~]]	+	[[Category: Raines DJ]]
-	[[Category: Turkenburg~~, J P~~]]	+	[[Category: Turkenburg JP]]
-	[[Category: Wilde~~, E J~~]]	+	[[Category: Wilde EJ]]
-	[[Category: Wilson~~, K S]]~~	+	[[Category: Wilson KS]]
-	~~[[Category: Ligand complex]]~~	+
-	~~[[Category: Periplasmic binding protein]]~~	+
-	~~[[Category: Synthetic siderophore]]~~	+
-	~~[[Category: Tetradentate siderophore]]~~	+
-	~~[[Category: Transport protein~~]]	+

OCA at 07:56, 26 June 2019

OCA — Wed, 26 Jun 2019 07:56:21 GMT

←Older revision		Revision as of 07:56, 26 June 2019
Line 1:		Line 1:

	==A complex of the synthetic siderophore analogue Fe(III)-5-LICAM with the CeuE periplasmic protein from Campylobacter jejuni==		==A complex of the synthetic siderophore analogue Fe(III)-5-LICAM with the CeuE periplasmic protein from Campylobacter jejuni==
-	<StructureSection load='5a5d' size='340' side='right' caption='[[5a5d]], [[Resolution\|resolution]] 1.74Å' scene=''>	+	<StructureSection load='5a5d' size='340' side='right'caption='[[5a5d]], [[Resolution\|resolution]] 1.74Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5a5d]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5A5D OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5A5D FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5a5d]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"campylobacter_fetus_subsp._jejuni"_smibert_1974 "campylobacter fetus subsp. jejuni" smibert 1974]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5A5D OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5A5D FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=5LC:N,N-PENTANE-1,5-DIYLBIS(2,3-DIHYDROXYBENZAMIDE)'>5LC</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=5LC:N,N-PENTANE-1,5-DIYLBIS(2,3-DIHYDROXYBENZAMIDE)'>5LC</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5a5d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5a5d OCA], [http://pdbe.org/5a5d PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5a5d RCSB], [http://www.ebi.ac.uk/pdbsum/5a5d PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5a5d ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5a5d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5a5d OCA], [http://pdbe.org/5a5d PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5a5d RCSB], [http://www.ebi.ac.uk/pdbsum/5a5d PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5a5d ProSAT]</span></td></tr>
Line 20:		Line 20:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Campylobacter fetus subsp. jejuni smibert 1974]]
		+	[[Category: Large Structures]]
	[[Category: Blagova, E]]		[[Category: Blagova, E]]
	[[Category: Duhme-Klair, A K]]		[[Category: Duhme-Klair, A K]]

OCA at 06:04, 19 April 2017

OCA — Wed, 19 Apr 2017 06:04:32 GMT

←Older revision		Revision as of 06:04, 19 April 2017
Line 7:		Line 7:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5a5d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5a5d OCA], [http://pdbe.org/5a5d PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5a5d RCSB], [http://www.ebi.ac.uk/pdbsum/5a5d PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5a5d ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5a5d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5a5d OCA], [http://pdbe.org/5a5d PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5a5d RCSB], [http://www.ebi.ac.uk/pdbsum/5a5d PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5a5d ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Bacteria use siderophores to mediate the transport of essential Fe(III) into the cell. In Campylobacter jejuni the periplasmic binding protein CeuE, an integral part of the Fe(III) transport system, has adapted to bind tetradentate siderophores using a His and a Tyr side chain to complete the Fe(III) coordination. A series of tetradentate siderophore mimics was synthesized in which the length of the linker between the two iron-binding catecholamide units was increased from four carbon atoms (4-LICAM4-) to five, six and eight (5-, 6-, 8-LICAM4-, respectively). Co-crystal structures with CeuE showed that the inter-planar angles between the iron-binding catecholamide units in the 5-, 6- and 8-LICAM4- structures are very similar (111 degrees , 110 degrees and 110 degrees ) and allow for an optimum fit into the binding pocket of CeuE, the inter-planar angle in the structure of 4-LICAM4- is significantly smaller (97 degrees ) due to restrictions imposed by the shorter linker. Accordingly, the protein-binding affinity was found to be slightly higher for 5- compared to 4-LICAM4- but decreases for 6- and 8-LICAM4-. The optimum linker length of five matches that present in natural siderophores such as enterobactin and azotochelin. Site-directed mutagenesis was used to investigate the relative importance of the Fe(III)-coordinating residues H227 and Y288.
		+
		+	Interactions of the periplasmic binding protein CeuE with Fe(III) n-LICAM4- siderophore analogues of varied linker length.,Wilde EJ, Hughes A, Blagova EV, Moroz OV, Thomas RP, Turkenburg JP, Raines DJ, Duhme-Klair AK, Wilson KS Sci Rep. 2017 Apr 6;7:45941. doi: 10.1038/srep45941. PMID:28383577<ref>PMID:28383577</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5a5d" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 09:01, 3 October 2016

OCA — Mon, 03 Oct 2016 09:01:57 GMT

←Older revision		Revision as of 09:01, 3 October 2016
Line 14:		Line 14:
	[[Category: Moroz, O V]]		[[Category: Moroz, O V]]
	[[Category: Raines, D J]]		[[Category: Raines, D J]]
-	[[Category: Turkenburg, J]]	+	[[Category: Turkenburg, J P]]
	[[Category: Wilde, E J]]		[[Category: Wilde, E J]]
	[[Category: Wilson, K S]]		[[Category: Wilson, K S]]

OCA at 10:16, 13 July 2016

OCA — Wed, 13 Jul 2016 10:16:55 GMT

←Older revision		Revision as of 10:16, 13 July 2016
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry 5a5d is ON HOLD until Paper Publication~~	+	==A complex of the synthetic siderophore analogue Fe(III)-5-LICAM with the CeuE periplasmic protein from Campylobacter jejuni==
-		+	<StructureSection load='5a5d' size='340' side='right' caption='[[5a5d]], [[Resolution\|resolution]] 1.74Å' scene=''>
-	~~Authors: Blagova, E., Hughes, A., Moroz, O.V., Raines, D.J., Wilde, E.J., Turkenburg, J., Duhme-Klair, A.-K., Wilson, K.S.~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5a5d]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5A5D OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5A5D FirstGlance]. <br>
-	~~Description:~~ A complex of the synthetic siderophore analogue Fe(III)-5-LICAM with the CeuE periplasmic protein from Campylobacter jejuni	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=5LC:N,N-PENTANE-1,5-DIYLBIS(2,3-DIHYDROXYBENZAMIDE)'>5LC</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5a5d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5a5d OCA], [http://pdbe.org/5a5d PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5a5d RCSB], [http://www.ebi.ac.uk/pdbsum/5a5d PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5a5d ProSAT]</span></td></tr>
-	[[~~Category~~: ~~Turkenburg, J~~]]	+	</table>
-	[[~~Category~~: ~~Wilson~~, K.S]]	+	__TOC__
		+	</StructureSection>
	[[Category: Blagova, E]]		[[Category: Blagova, E]]
-	[[Category: Duhme-Klair, A.-K]]	+	[[Category: Duhme-Klair, A K]]
	[[Category: Hughes, A]]		[[Category: Hughes, A]]
-	[[Category: Raines, D.J]]	+	[[Category: Moroz, O V]]
-	[[Category: Wilde, E.J]]	+	[[Category: Raines, D J]]
-	[[Category: ~~Moroz~~, ~~O.V~~]]	+	[[Category: Turkenburg, J]]
		+	[[Category: Wilde, E J]]
		+	[[Category: Wilson, K S]]
		+	[[Category: Ligand complex]]
		+	[[Category: Periplasmic binding protein]]
		+	[[Category: Synthetic siderophore]]
		+	[[Category: Tetradentate siderophore]]
		+	[[Category: Transport protein]]

OCA at 00:20, 1 December 2015

OCA — Tue, 01 Dec 2015 00:20:30 GMT

←Older revision		Revision as of 00:20, 1 December 2015
Line 9:		Line 9:
	[[Category: Turkenburg, J]]		[[Category: Turkenburg, J]]
	[[Category: Wilson, K.S]]		[[Category: Wilson, K.S]]
		+	[[Category: Blagova, E]]
	[[Category: Duhme-Klair, A.-K]]		[[Category: Duhme-Klair, A.-K]]
	[[Category: Hughes, A]]		[[Category: Hughes, A]]
	[[Category: Raines, D.J]]		[[Category: Raines, D.J]]
-	[[Category: Blagova, E]]
	[[Category: Wilde, E.J]]		[[Category: Wilde, E.J]]
	[[Category: Moroz, O.V]]		[[Category: Moroz, O.V]]

OCA at 10:27, 1 July 2015

OCA — Wed, 01 Jul 2015 10:27:22 GMT

←Older revision		Revision as of 10:27, 1 July 2015
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5a5d is ON HOLD	+	The entry 5a5d is ON HOLD until Paper Publication

	Authors: Blagova, E., Hughes, A., Moroz, O.V., Raines, D.J., Wilde, E.J., Turkenburg, J., Duhme-Klair, A.-K., Wilson, K.S.		Authors: Blagova, E., Hughes, A., Moroz, O.V., Raines, D.J., Wilde, E.J., Turkenburg, J., Duhme-Klair, A.-K., Wilson, K.S.

OCA: Protected "5a5d" [edit=sysop:move=sysop]

OCA — Wed, 24 Jun 2015 11:19:47 GMT

Protected "5a5d" [edit=sysop:move=sysop]

←Older revision

Revision as of 11:19, 24 June 2015

OCA: New page: '''Unreleased structure''' The entry 5a5d is ON HOLD Authors: Blagova, E., Hughes, A., Moroz, O.V., Raines, D.J., Wilde, E.J., Turkenburg, J., Duhme-Klair, A.-K., Wilson, K.S. Descript...

OCA — Wed, 24 Jun 2015 11:19:46 GMT

New page: '''Unreleased structure''' The entry 5a5d is ON HOLD Authors: Blagova, E., Hughes, A., Moroz, O.V., Raines, D.J., Wilde, E.J., Turkenburg, J., Duhme-Klair, A.-K., Wilson, K.S. Descript...

New page

'''Unreleased structure'''

The entry 5a5d is ON HOLD

Authors: Blagova, E., Hughes, A., Moroz, O.V., Raines, D.J., Wilde, E.J., Turkenburg, J., Duhme-Klair, A.-K., Wilson, K.S.

Description: A complex of the synthetic siderophore analogue Fe(III)-5-LICAM with the CeuE periplasmic protein from Campylobacter jejuni
[[Category: Unreleased Structures]]
[[Category: Turkenburg, J]]
[[Category: Wilson, K.S]]
[[Category: Duhme-Klair, A.-K]]
[[Category: Hughes, A]]
[[Category: Raines, D.J]]
[[Category: Blagova, E]]
[[Category: Wilde, E.J]]
[[Category: Moroz, O.V]]