5aa3 - Revision history

OCA at 11:06, 10 January 2024

OCA — Wed, 10 Jan 2024 11:06:21 GMT

←Older revision		Revision as of 11:06, 10 January 2024
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5aa3]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_BWHPSA013 Pseudomonas aeruginosa BWHPSA013]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5AA3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5AA3 FirstGlance]. <br>		<table><tr><td colspan='2'>[[5aa3]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_BWHPSA013 Pseudomonas aeruginosa BWHPSA013]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5AA3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5AA3 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GLU:GLUTAMIC+ACID'>GLU</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.2Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GLU:GLUTAMIC+ACID'>GLU</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5aa3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5aa3 OCA], [https://pdbe.org/5aa3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5aa3 RCSB], [https://www.ebi.ac.uk/pdbsum/5aa3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5aa3 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5aa3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5aa3 OCA], [https://pdbe.org/5aa3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5aa3 RCSB], [https://www.ebi.ac.uk/pdbsum/5aa3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5aa3 ProSAT]</span></td></tr>
	</table>		</table>

OCA at 04:47, 25 May 2023

OCA — Thu, 25 May 2023 04:47:30 GMT

←Older revision		Revision as of 04:47, 25 May 2023
Line 3:		Line 3:
	<StructureSection load='5aa3' size='340' side='right'caption='[[5aa3]], [[Resolution\|resolution]] 3.20Å' scene=''>		<StructureSection load='5aa3' size='340' side='right'caption='[[5aa3]], [[Resolution\|resolution]] 3.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5aa3]] is a 12 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Pseudomonas_aeruginosa_bwhpsa013~~ Pseudomonas aeruginosa ~~bwhpsa013~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5AA3 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5AA3 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5aa3]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_BWHPSA013 Pseudomonas aeruginosa BWHPSA013]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5AA3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5AA3 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GLU:GLUTAMIC+ACID'>GLU</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GLU:GLUTAMIC+ACID'>GLU</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5aa1\|5aa1]], [[5aa2\|5aa2]], [[5aa4\|5aa4]]~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5aa3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5aa3 OCA], [https://pdbe.org/5aa3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5aa3 RCSB], [https://www.ebi.ac.uk/pdbsum/5aa3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5aa3 ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5aa3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5aa3 OCA], [~~http~~://pdbe.org/5aa3 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5aa3 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5aa3 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5aa3 ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/MLTF_PSEAE MLTF_PSEAE]] Murein-degrading enzyme that degrades murein glycan strands and insoluble, high-molecular weight murein sacculi, with the concomitant formation of a 1,6-anhydromuramoyl product. Lytic transglycosylases (LTs) play an integral role in the metabolism of the peptidoglycan (PG) sacculus. Their lytic action creates space within the PG sacculus to allow for its expansion as well as for the insertion of various structures such as secretion systems and flagella.[HAMAP-Rule:MF_02016]	+	[https://www.uniprot.org/uniprot/MLTF_PSEAE MLTF_PSEAE] Murein-degrading enzyme that degrades murein glycan strands and insoluble, high-molecular weight murein sacculi, with the concomitant formation of a 1,6-anhydromuramoyl product. Lytic transglycosylases (LTs) play an integral role in the metabolism of the peptidoglycan (PG) sacculus. Their lytic action creates space within the PG sacculus to allow for its expansion as well as for the insertion of various structures such as secretion systems and flagella.[HAMAP-Rule:MF_02016]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Pseudomonas aeruginosa ~~bwhpsa013~~]]	+	[[Category: Pseudomonas aeruginosa BWHPSA013]]
-	[[Category: Acebron, I]]	+	[[Category: Acebron I]]
-	[[Category: Dominguez-Gil, T]]	+	[[Category: Dominguez-Gil T]]
-	[[Category: Hermoso~~, J A]]~~	+	[[Category: Hermoso JA]]
-	~~[[Category: Cell wall recycling]]~~	+
-	~~[[Category: Lytic transglycosilase]]~~	+
-	~~[[Category: Pseudomonas aeruginosa]]~~	+
-	~~[[Category: Sugar binding protein~~]]	+

OCA at 08:06, 26 June 2019

OCA — Wed, 26 Jun 2019 08:06:10 GMT

←Older revision		Revision as of 08:06, 26 June 2019
Line 1:		Line 1:
-	~~{{Large structure}}~~	+
	==Crystal structure of MltF from Pseudomonas aeruginosa in the presence of tetrasaccharide and tetrapeptide==		==Crystal structure of MltF from Pseudomonas aeruginosa in the presence of tetrasaccharide and tetrapeptide==
-	<StructureSection load='5aa3' size='340' side='right' caption='[[5aa3]], [[Resolution\|resolution]] 3.20Å' scene=''>	+	<StructureSection load='5aa3' size='340' side='right'caption='[[5aa3]], [[Resolution\|resolution]] 3.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5aa3]] is a 12 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5AA3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5AA3 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5aa3]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_bwhpsa013 Pseudomonas aeruginosa bwhpsa013]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5AA3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5AA3 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GLU:GLUTAMIC+ACID'>GLU</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GLU:GLUTAMIC+ACID'>GLU</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5aa1\|5aa1]], [[5aa2\|5aa2]], [[5aa4\|5aa4]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5aa1\|5aa1]], [[5aa2\|5aa2]], [[5aa4\|5aa4]]</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5aa3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5aa3 OCA], [http://pdbe.org/5aa3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5aa3 RCSB], [http://www.ebi.ac.uk/pdbsum/5aa3 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5aa3 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5aa3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5aa3 OCA], [http://pdbe.org/5aa3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5aa3 RCSB], [http://www.ebi.ac.uk/pdbsum/5aa3 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5aa3 ProSAT]</span></td></tr>
	</table>		</table>
-	{{Large structure}}
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/MLTF_PSEAE MLTF_PSEAE]] Murein-degrading enzyme that degrades murein glycan strands and insoluble, high-molecular weight murein sacculi, with the concomitant formation of a 1,6-anhydromuramoyl product. Lytic transglycosylases (LTs) play an integral role in the metabolism of the peptidoglycan (PG) sacculus. Their lytic action creates space within the PG sacculus to allow for its expansion as well as for the insertion of various structures such as secretion systems and flagella.[HAMAP-Rule:MF_02016]		[[http://www.uniprot.org/uniprot/MLTF_PSEAE MLTF_PSEAE]] Murein-degrading enzyme that degrades murein glycan strands and insoluble, high-molecular weight murein sacculi, with the concomitant formation of a 1,6-anhydromuramoyl product. Lytic transglycosylases (LTs) play an integral role in the metabolism of the peptidoglycan (PG) sacculus. Their lytic action creates space within the PG sacculus to allow for its expansion as well as for the insertion of various structures such as secretion systems and flagella.[HAMAP-Rule:MF_02016]
Line 24:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Pseudomonas aeruginosa bwhpsa013]]
	[[Category: Acebron, I]]		[[Category: Acebron, I]]
	[[Category: Dominguez-Gil, T]]		[[Category: Dominguez-Gil, T]]

OCA at 17:36, 19 October 2016

OCA — Wed, 19 Oct 2016 17:36:17 GMT

←Older revision		Revision as of 17:36, 19 October 2016
Line 1:		Line 1:
-	'''~~Unreleased~~ structure'''	+	{{Large structure}}
		+	==Crystal structure of MltF from Pseudomonas aeruginosa in the presence of tetrasaccharide and tetrapeptide==
		+	<StructureSection load='5aa3' size='340' side='right' caption='[[5aa3]], [[Resolution\|resolution]] 3.20Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5aa3]] is a 12 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5AA3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5AA3 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GLU:GLUTAMIC+ACID'>GLU</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5aa1\|5aa1]], [[5aa2\|5aa2]], [[5aa4\|5aa4]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5aa3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5aa3 OCA], [http://pdbe.org/5aa3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5aa3 RCSB], [http://www.ebi.ac.uk/pdbsum/5aa3 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5aa3 ProSAT]</span></td></tr>
		+	</table>
		+	{{Large structure}}
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/MLTF_PSEAE MLTF_PSEAE]] Murein-degrading enzyme that degrades murein glycan strands and insoluble, high-molecular weight murein sacculi, with the concomitant formation of a 1,6-anhydromuramoyl product. Lytic transglycosylases (LTs) play an integral role in the metabolism of the peptidoglycan (PG) sacculus. Their lytic action creates space within the PG sacculus to allow for its expansion as well as for the insertion of various structures such as secretion systems and flagella.[HAMAP-Rule:MF_02016]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Bacteria grow and divide without loss of cellular integrity. This accomplishment is notable, as a key component of their cell envelope is a surrounding glycopeptide polymer. In Gram-negative bacteria this polymer-the peptidoglycan-grows by the difference between concurrent synthesis and degradation. The regulation of the enzymatic ensemble for these activities is poorly understood. We report herein the structural basis for the control of one such enzyme, the lytic transglycosylase MltF of Pseudomonas aeruginosa. Its structure comprises two modules: an ABC-transporter-like regulatory module and a catalytic module. Occupancy of the regulatory module by peptidoglycan-derived muropeptides effects a dramatic and long-distance (40 A) conformational change, occurring over the entire protein structure, to open its active site for catalysis. This discovery of the molecular basis for the allosteric control of MltF catalysis is foundational to further study of MltF within the complex enzymatic orchestration of the dynamic peptidoglycan.

-	~~The entry 5aa3 is ON HOLD until Paper Publication~~	+	Activation by Allostery in Cell-Wall Remodeling by a Modular Membrane-Bound Lytic Transglycosylase from Pseudomonas aeruginosa.,Dominguez-Gil T, Lee M, Acebron-Avalos I, Mahasenan KV, Hesek D, Dik DA, Byun B, Lastochkin E, Fisher JF, Mobashery S, Hermoso JA Structure. 2016 Oct 4;24(10):1729-1741. doi: 10.1016/j.str.2016.07.019. Epub 2016, Sep 8. PMID:27618662<ref>PMID:27618662</ref>

-	~~Authors: Dominguez-Gil~~, T.~~, Acebron, I~~.~~, Hermoso, J.A~~.	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~Description~~: ~~Crystal structure of MltF from Pseudomonas aeruginosa in the presence of tetrasaccharide and tetrapeptide~~	+	<div class="pdbe-citations 5aa3" style="background-color:#fffaf0;"></div>
-	~~[[Category: Unreleased Structures]]~~	+	== References ==
-	[[Category: ~~Hermoso~~, ~~J.A~~]]	+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Acebron, I]]
	[[Category: Dominguez-Gil, T]]		[[Category: Dominguez-Gil, T]]
-	[[Category: ~~Acebron~~, I]]	+	[[Category: Hermoso, J A]]
		+	[[Category: Cell wall recycling]]
		+	[[Category: Lytic transglycosilase]]
		+	[[Category: Pseudomonas aeruginosa]]
		+	[[Category: Sugar binding protein]]

OCA at 00:25, 1 December 2015

OCA — Tue, 01 Dec 2015 00:25:05 GMT

←Older revision		Revision as of 00:25, 1 December 2015
Line 7:		Line 7:
	Description: Crystal structure of MltF from Pseudomonas aeruginosa in the presence of tetrasaccharide and tetrapeptide		Description: Crystal structure of MltF from Pseudomonas aeruginosa in the presence of tetrasaccharide and tetrapeptide
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
		+	[[Category: Hermoso, J.A]]
	[[Category: Dominguez-Gil, T]]		[[Category: Dominguez-Gil, T]]
	[[Category: Acebron, I]]		[[Category: Acebron, I]]
-	[[Category: Hermoso, J.A]]

OCA at 02:07, 22 October 2015

OCA — Thu, 22 Oct 2015 02:07:37 GMT

←Older revision		Revision as of 02:07, 22 October 2015
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5aa3 is ON HOLD	+	The entry 5aa3 is ON HOLD until Paper Publication

	Authors: Dominguez-Gil, T., Acebron, I., Hermoso, J.A.		Authors: Dominguez-Gil, T., Acebron, I., Hermoso, J.A.

OCA at 17:33, 5 August 2015

OCA — Wed, 05 Aug 2015 17:33:58 GMT

←Older revision		Revision as of 17:33, 5 August 2015
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5aa3 is ON HOLD ~~until sometime in the future~~	+	The entry 5aa3 is ON HOLD

	Authors: Dominguez-Gil, T., Acebron, I., Hermoso, J.A.		Authors: Dominguez-Gil, T., Acebron, I., Hermoso, J.A.

OCA: Protected "5aa3" [edit=sysop:move=sysop]

OCA — Wed, 29 Jul 2015 12:27:30 GMT

Protected "5aa3" [edit=sysop:move=sysop]

←Older revision

Revision as of 12:27, 29 July 2015

OCA: New page: '''Unreleased structure''' The entry 5aa3 is ON HOLD until sometime in the future Authors: Dominguez-Gil, T., Acebron, I., Hermoso, J.A. Description: Crystal structure of MltF from Pse...

OCA — Wed, 29 Jul 2015 12:27:28 GMT

New page: '''Unreleased structure''' The entry 5aa3 is ON HOLD until sometime in the future Authors: Dominguez-Gil, T., Acebron, I., Hermoso, J.A. Description: Crystal structure of MltF from Pse...

New page

'''Unreleased structure'''

The entry 5aa3 is ON HOLD until sometime in the future

Authors: Dominguez-Gil, T., Acebron, I., Hermoso, J.A.

Description: Crystal structure of MltF from Pseudomonas aeruginosa in the presence of tetrasaccharide and tetrapeptide
[[Category: Unreleased Structures]]
[[Category: Dominguez-Gil, T]]
[[Category: Acebron, I]]
[[Category: Hermoso, J.A]]