5ac4 - Revision history

OCA at 11:07, 10 January 2024

OCA — Wed, 10 Jan 2024 11:07:11 GMT

←Older revision		Revision as of 11:07, 10 January 2024
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5ac4]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_pneumoniae_TIGR4 Streptococcus pneumoniae TIGR4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5AC4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5AC4 FirstGlance]. <br>		<table><tr><td colspan='2'>[[5ac4]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_pneumoniae_TIGR4 Streptococcus pneumoniae TIGR4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5AC4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5AC4 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NGA:N-ACETYL-D-GALACTOSAMINE'>NGA</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.08Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NGA:N-ACETYL-D-GALACTOSAMINE'>NGA</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ac4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ac4 OCA], [https://pdbe.org/5ac4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ac4 RCSB], [https://www.ebi.ac.uk/pdbsum/5ac4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ac4 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ac4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ac4 OCA], [https://pdbe.org/5ac4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ac4 RCSB], [https://www.ebi.ac.uk/pdbsum/5ac4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ac4 ProSAT]</span></td></tr>
	</table>		</table>

OCA at 04:50, 25 May 2023

OCA — Thu, 25 May 2023 04:50:30 GMT

←Older revision		Revision as of 04:50, 25 May 2023
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	<StructureSection load='5ac4' size='340' side='right'caption='[[5ac4]], [[Resolution\|resolution]] 2.08Å' scene=''>		<StructureSection load='5ac4' size='340' side='right'caption='[[5ac4]], [[Resolution\|resolution]] 2.08Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5ac4]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Strpn Strpn~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5AC4 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5AC4 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5ac4]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_pneumoniae_TIGR4 Streptococcus pneumoniae TIGR4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5AC4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5AC4 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NGA:N-ACETYL-D-GALACTOSAMINE'>NGA</scene~~></td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NGA:N-ACETYL-D-GALACTOSAMINE'>NGA</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5ac5\|5ac5]]</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ac4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ac4 OCA], [https://pdbe.org/5ac4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ac4 RCSB], [https://www.ebi.ac.uk/pdbsum/5ac4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ac4 ProSAT]</span></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-N-acetylhexosaminidase Beta-N-acetylhexosaminidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.52 3.2.1.52] </span></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5ac4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ac4 OCA], [~~http~~://pdbe.org/5ac4 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5ac4 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5ac4 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5ac4 ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A0A0H2US73_STRPN A0A0H2US73_STRPN]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 22:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Beta-N-acetylhexosaminidase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Strpn~~]]	+	[[Category: Streptococcus pneumoniae TIGR4]]
-	[[Category: Boraston~~, A B~~]]	+	[[Category: Boraston AB]]
-	[[Category: Cid, M]]	+	[[Category: Cid M]]
-	[[Category: Higgins~~, M A~~]]	+	[[Category: Higgins MA]]
-	[[Category: Robb~~, C S]]~~	+	[[Category: Robb CS]]
-	~~[[Category: Bacterial protein]]~~	+
-	~~[[Category: Carbohydrate conformation]]~~	+
-	~~[[Category: Catalytic domain]]~~	+
-	~~[[Category: Host-pathogen interaction]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Hydrolysis]]~~	+
-	~~[[Category: Polysaccharide]]~~	+
-	~~[[Category: Virulence factor~~]]	+

OCA at 08:01, 4 March 2020

OCA — Wed, 04 Mar 2020 08:01:29 GMT

←Older revision		Revision as of 08:01, 4 March 2020
Line 1:		Line 1:

	==GH20C, Beta-hexosaminidase from Streptococcus pneumoniae in complex with GalNAc==		==GH20C, Beta-hexosaminidase from Streptococcus pneumoniae in complex with GalNAc==
-	<StructureSection load='5ac4' size='340' side='right' caption='[[5ac4]], [[Resolution\|resolution]] 2.08Å' scene=''>	+	<StructureSection load='5ac4' size='340' side='right'caption='[[5ac4]], [[Resolution\|resolution]] 2.08Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5ac4]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Strpn Strpn]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5AC4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5AC4 FirstGlance]. <br>		<table><tr><td colspan='2'>[[5ac4]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Strpn Strpn]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5AC4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5AC4 FirstGlance]. <br>
Line 23:		Line 23:
	</StructureSection>		</StructureSection>
	[[Category: Beta-N-acetylhexosaminidase]]		[[Category: Beta-N-acetylhexosaminidase]]
		+	[[Category: Large Structures]]
	[[Category: Strpn]]		[[Category: Strpn]]
	[[Category: Boraston, A B]]		[[Category: Boraston, A B]]

OCA at 15:35, 16 November 2017

OCA — Thu, 16 Nov 2017 15:35:33 GMT

←Older revision		Revision as of 15:35, 16 November 2017
Line 3:		Line 3:
	<StructureSection load='5ac4' size='340' side='right' caption='[[5ac4]], [[Resolution\|resolution]] 2.08Å' scene=''>		<StructureSection load='5ac4' size='340' side='right' caption='[[5ac4]], [[Resolution\|resolution]] 2.08Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5ac4]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5AC4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5AC4 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5ac4]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Strpn Strpn]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5AC4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5AC4 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NGA:N-ACETYL-D-GALACTOSAMINE'>NGA</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NGA:N-ACETYL-D-GALACTOSAMINE'>NGA</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5ac5\|5ac5]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5ac5\|5ac5]]</td></tr>
Line 23:		Line 23:
	</StructureSection>		</StructureSection>
	[[Category: Beta-N-acetylhexosaminidase]]		[[Category: Beta-N-acetylhexosaminidase]]
		+	[[Category: Strpn]]
	[[Category: Boraston, A B]]		[[Category: Boraston, A B]]
	[[Category: Cid, M]]		[[Category: Cid, M]]

OCA at 09:54, 22 March 2017

OCA — Wed, 22 Mar 2017 09:54:17 GMT

←Older revision		Revision as of 09:54, 22 March 2017
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5ac4 is ~~ON HOLD until Paper~~ Publication	+	==GH20C, Beta-hexosaminidase from Streptococcus pneumoniae in complex with GalNAc==
		+	<StructureSection load='5ac4' size='340' side='right' caption='[[5ac4]], [[Resolution\|resolution]] 2.08Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5ac4]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5AC4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5AC4 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NGA:N-ACETYL-D-GALACTOSAMINE'>NGA</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5ac5\|5ac5]]</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-N-acetylhexosaminidase Beta-N-acetylhexosaminidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.52 3.2.1.52] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ac4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ac4 OCA], [http://pdbe.org/5ac4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ac4 RCSB], [http://www.ebi.ac.uk/pdbsum/5ac4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ac4 ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	An important facet of the interaction between the pathogen Streptococcus pneumoniae (pneumococcus) and its human host is the ability of this bacterium to process host glycans. To achieve cleavage of the glycosidic bonds in host glycans, S. pneumoniae deploys a wide array of glycoside hydrolases. Here, we identify and characterize a new family 20 glycoside hydrolase, GH20C, from S. pneumoniae. Recombinant GH20C possessed the ability to hydrolyze the beta-linkages joining either N-acetylglucosamine or N-acetylgalactosamine to a wide variety of aglycon residues, thus revealing this enzyme to be a generalist N-acetylhexosaminidase in vitro. X-ray crystal structures were determined for GH20C in a ligand-free form, in complex with the N-acetylglucosamine and N-acetylgalactosamine products of catalysis and in complex with both gluco- and galacto-configured inhibitors O-(2-acetamido-2-deoxy-D-glucopyranosylidene)amino N-phenyl carbamate (PUGNAc), O-(2-acetamido-2-deoxy-D-galactopyranosylidene)amino N-phenyl carbamate (GalPUGNAc), N-acetyl-D-glucosamine-thiazoline (NGT), and N-acetyl-D-galactosamine-thiazoline (GalNGT) at resolutions from 1.84 to 2.7 A. These structures showed N-acetylglucosamine and N-acetylgalactosamine to be recognized via identical sets of molecular interactions. Although the same sets of interaction were maintained with the gluco- and galacto-configured inhibitors, the inhibition constants suggested preferred recognition of the axial O4 when an aglycon moiety was present (Ki for PUGNAc > GalPUGNAc) but preferred recognition of an equatorial O4 when the aglycon was absent (Ki for GalNGT > NGT). Overall, this study reveals GH20C to be another tool that is unique in the arsenal of S. pneumoniae and that it may implement the effort of the bacterium to utilize and/or destroy the wide array of host glycans that it may encounter.

-	~~Authors: Cid~~, M., Robb~~, C.S.~~, Higgins, ~~M.A.~~, ~~Boraston~~, A.B.	+	A Second beta-Hexosaminidase Encoded in the Streptococcus pneumoniae Genome Provides an Expanded Biochemical Ability to Degrade Host Glycans.,Robb M, Robb CS, Higgins MA, Hobbs JK, Paton JC, Boraston AB J Biol Chem. 2015 Dec 25;290(52):30888-900. doi: 10.1074/jbc.M115.688630. Epub, 2015 Oct 21. PMID:26491009<ref>PMID:26491009</ref>

-	~~Description: GH20C~~, ~~Beta~~-~~hexosaminidase from Streptococcus pneumoniae in complex with GalNAc~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
-	[[Category: ~~Robb~~, ~~C.S~~]]	+	<div class="pdbe-citations 5ac4" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Beta-N-acetylhexosaminidase]]
		+	[[Category: Boraston, A B]]
	[[Category: Cid, M]]		[[Category: Cid, M]]
-	[[Category: ~~Boraston~~, A.B]]	+	[[Category: Higgins, M A]]
-	[[Category: ~~Higgins~~, ~~M.A~~]]	+	[[Category: Robb, C S]]
		+	[[Category: Bacterial protein]]
		+	[[Category: Carbohydrate conformation]]
		+	[[Category: Catalytic domain]]
		+	[[Category: Host-pathogen interaction]]
		+	[[Category: Hydrolase]]
		+	[[Category: Hydrolysis]]
		+	[[Category: Polysaccharide]]
		+	[[Category: Virulence factor]]

OCA at 09:45, 2 September 2015

OCA — Wed, 02 Sep 2015 09:45:00 GMT

←Older revision		Revision as of 09:45, 2 September 2015
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5ac4 is ON HOLD	+	The entry 5ac4 is ON HOLD until Paper Publication

	Authors: Cid, M., Robb, C.S., Higgins, M.A., Boraston, A.B.		Authors: Cid, M., Robb, C.S., Higgins, M.A., Boraston, A.B.

OCA: Protected "5ac4" [edit=sysop:move=sysop]

OCA — Thu, 20 Aug 2015 09:54:24 GMT

Protected "5ac4" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:54, 20 August 2015

OCA: New page: '''Unreleased structure''' The entry 5ac4 is ON HOLD Authors: Cid, M., Robb, C.S., Higgins, M.A., Boraston, A.B. Description: GH20C, Beta-hexosaminidase from Streptococcus pneumoniae i...

OCA — Thu, 20 Aug 2015 09:54:23 GMT

New page: '''Unreleased structure''' The entry 5ac4 is ON HOLD Authors: Cid, M., Robb, C.S., Higgins, M.A., Boraston, A.B. Description: GH20C, Beta-hexosaminidase from Streptococcus pneumoniae i...

New page

'''Unreleased structure'''

The entry 5ac4 is ON HOLD

Authors: Cid, M., Robb, C.S., Higgins, M.A., Boraston, A.B.

Description: GH20C, Beta-hexosaminidase from Streptococcus pneumoniae in complex with GalNAc
[[Category: Unreleased Structures]]
[[Category: Robb, C.S]]
[[Category: Cid, M]]
[[Category: Boraston, A.B]]
[[Category: Higgins, M.A]]