5aex - Revision history

OCA at 11:08, 10 January 2024

OCA — Wed, 10 Jan 2024 11:08:33 GMT

←Older revision		Revision as of 11:08, 10 January 2024
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5aex]] is a 9 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5AEX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5AEX FirstGlance]. <br>		<table><tr><td colspan='2'>[[5aex]] is a 9 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5AEX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5AEX FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.2Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5aex FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5aex OCA], [https://pdbe.org/5aex PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5aex RCSB], [https://www.ebi.ac.uk/pdbsum/5aex PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5aex ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5aex FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5aex OCA], [https://pdbe.org/5aex PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5aex RCSB], [https://www.ebi.ac.uk/pdbsum/5aex PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5aex ProSAT]</span></td></tr>
	</table>		</table>

OCA at 04:54, 25 May 2023

OCA — Thu, 25 May 2023 04:54:31 GMT

←Older revision		Revision as of 04:54, 25 May 2023
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	<StructureSection load='5aex' size='340' side='right'caption='[[5aex]], [[Resolution\|resolution]] 3.20Å' scene=''>		<StructureSection load='5aex' size='340' side='right'caption='[[5aex]], [[Resolution\|resolution]] 3.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5aex]] is a 9 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Atcc_18824 Atcc 18824~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5AEX OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5AEX FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5aex]] is a 9 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5AEX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5AEX FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5aez\|5aez]], [[5af1\|5af1]]~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5aex FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5aex OCA], [https://pdbe.org/5aex PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5aex RCSB], [https://www.ebi.ac.uk/pdbsum/5aex PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5aex ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5aex FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5aex OCA], [~~http~~://pdbe.org/5aex PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5aex RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5aex PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5aex ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/MEP2_YEAST MEP2_YEAST]] Transporter for ammonium (both charged and uncharged NH3 and NH4) to use as a nitrogen source. The affinity of MEP2 is about twenty times higher than that of MEP1. MEP3 has the lowest affinity. Under ammonium limitation acts as an ammonium sensor, generating a signal that leads to pseudohyphal growth.<ref>PMID:11069679</ref> <ref>PMID:11486013</ref> <ref>PMID:9234685</ref> <ref>PMID:9482721</ref>	+	[https://www.uniprot.org/uniprot/MEP2_YEAST MEP2_YEAST] Transporter for ammonium (both charged and uncharged NH3 and NH4) to use as a nitrogen source. The affinity of MEP2 is about twenty times higher than that of MEP1. MEP3 has the lowest affinity. Under ammonium limitation acts as an ammonium sensor, generating a signal that leads to pseudohyphal growth.<ref>PMID:11069679</ref> <ref>PMID:11486013</ref> <ref>PMID:9234685</ref> <ref>PMID:9482721</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Atcc 18824]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Berg, B van den~~]]	+	[[Category: Saccharomyces cerevisiae]]
-	[[Category: Chembath, A]]	+	[[Category: Chembath A]]
-	[[Category: Rutherford~~, J C~~]]	+	[[Category: Rutherford JC]]
-	[[Category: ~~Membrane protein~~]]	+	[[Category: Van den Berg B]]

OCA at 07:46, 3 July 2019

OCA — Wed, 03 Jul 2019 07:46:45 GMT

←Older revision		Revision as of 07:46, 3 July 2019
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	==Crystal structure of Saccharomyces cerevisiae Mep2==		==Crystal structure of Saccharomyces cerevisiae Mep2==
-	<StructureSection load='5aex' size='340' side='right' caption='[[5aex]], [[Resolution\|resolution]] 3.20Å' scene=''>	+	<StructureSection load='5aex' size='340' side='right'caption='[[5aex]], [[Resolution\|resolution]] 3.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5aex]] is a 9 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5AEX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5AEX FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5aex]] is a 9 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5AEX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5AEX FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5aez\|5aez]], [[5af1\|5af1]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5aez\|5aez]], [[5af1\|5af1]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5aex FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5aex OCA], [http://pdbe.org/5aex PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5aex RCSB], [http://www.ebi.ac.uk/pdbsum/5aex PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5aex FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5aex OCA], [http://pdbe.org/5aex PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5aex RCSB], [http://www.ebi.ac.uk/pdbsum/5aex PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5aex ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 23:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Atcc 18824]]
		+	[[Category: Large Structures]]
	[[Category: Berg, B van den]]		[[Category: Berg, B van den]]
	[[Category: Chembath, A]]		[[Category: Chembath, A]]
	[[Category: Rutherford, J C]]		[[Category: Rutherford, J C]]
	[[Category: Membrane protein]]		[[Category: Membrane protein]]

OCA at 19:19, 10 May 2016

OCA — Tue, 10 May 2016 19:19:41 GMT

←Older revision		Revision as of 19:19, 10 May 2016
Line 1:		Line 1:
		+
	==Crystal structure of Saccharomyces cerevisiae Mep2==		==Crystal structure of Saccharomyces cerevisiae Mep2==
	<StructureSection load='5aex' size='340' side='right' caption='[[5aex]], [[Resolution\|resolution]] 3.20Å' scene=''>		<StructureSection load='5aex' size='340' side='right' caption='[[5aex]], [[Resolution\|resolution]] 3.20Å' scene=''>
Line 9:		Line 10:
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/MEP2_YEAST MEP2_YEAST]] Transporter for ammonium (both charged and uncharged NH3 and NH4) to use as a nitrogen source. The affinity of MEP2 is about twenty times higher than that of MEP1. MEP3 has the lowest affinity. Under ammonium limitation acts as an ammonium sensor, generating a signal that leads to pseudohyphal growth.<ref>PMID:11069679</ref> <ref>PMID:11486013</ref> <ref>PMID:9234685</ref> <ref>PMID:9482721</ref>		[[http://www.uniprot.org/uniprot/MEP2_YEAST MEP2_YEAST]] Transporter for ammonium (both charged and uncharged NH3 and NH4) to use as a nitrogen source. The affinity of MEP2 is about twenty times higher than that of MEP1. MEP3 has the lowest affinity. Under ammonium limitation acts as an ammonium sensor, generating a signal that leads to pseudohyphal growth.<ref>PMID:11069679</ref> <ref>PMID:11486013</ref> <ref>PMID:9234685</ref> <ref>PMID:9482721</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Mep2 proteins are fungal transceptors that play an important role as ammonium sensors in fungal development. Mep2 activity is tightly regulated by phosphorylation, but how this is achieved at the molecular level is not clear. Here we report X-ray crystal structures of the Mep2 orthologues from Saccharomyces cerevisiae and Candida albicans and show that under nitrogen-sufficient conditions the transporters are not phosphorylated and present in closed, inactive conformations. Relative to the open bacterial ammonium transporters, non-phosphorylated Mep2 exhibits shifts in cytoplasmic loops and the C-terminal region (CTR) to occlude the cytoplasmic exit of the channel and to interact with His2 of the twin-His motif. The phosphorylation site in the CTR is solvent accessible and located in a negatively charged pocket approximately 30 A away from the channel exit. The crystal structure of phosphorylation-mimicking Mep2 variants from C. albicans show large conformational changes in a conserved and functionally important region of the CTR. The results allow us to propose a model for regulation of eukaryotic ammonium transport by phosphorylation.
		+
		+	Structural basis for Mep2 ammonium transceptor activation by phosphorylation.,van den Berg B, Chembath A, Jefferies D, Basle A, Khalid S, Rutherford JC Nat Commun. 2016 Apr 18;7:11337. doi: 10.1038/ncomms11337. PMID:27088325<ref>PMID:27088325</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5aex" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 17:16, 20 January 2016

OCA — Wed, 20 Jan 2016 17:16:07 GMT

←Older revision		Revision as of 17:16, 20 January 2016
Line 1:		Line 1:
-	'''~~Unreleased structure~~'''	+	==Crystal structure of Saccharomyces cerevisiae Mep2==
-		+	<StructureSection load='5aex' size='340' side='right' caption='[[5aex]], [[Resolution\|resolution]] 3.20Å' scene=''>
-	~~The entry~~ 5aex is ~~ON HOLD until Paper Publication~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5aex]] is a 9 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5AEX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5AEX FirstGlance]. <br>
-	~~Authors~~: ~~Rutherford~~, J.C., ~~Chembath~~, A., ~~van den Berg~~, B.	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
-		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5aez\|5aez]], [[5af1\|5af1]]</td></tr>
-	~~Description: Crystal structure of Saccharomyces cerevisiae Mep2~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5aex FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5aex OCA], [http://pdbe.org/5aex PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5aex RCSB], [http://www.ebi.ac.uk/pdbsum/5aex PDBsum]</span></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	</table>
-	[[Category: ~~Van Den~~ Berg, B]]	+	== Function ==
		+	[[http://www.uniprot.org/uniprot/MEP2_YEAST MEP2_YEAST]] Transporter for ammonium (both charged and uncharged NH3 and NH4) to use as a nitrogen source. The affinity of MEP2 is about twenty times higher than that of MEP1. MEP3 has the lowest affinity. Under ammonium limitation acts as an ammonium sensor, generating a signal that leads to pseudohyphal growth.<ref>PMID:11069679</ref> <ref>PMID:11486013</ref> <ref>PMID:9234685</ref> <ref>PMID:9482721</ref>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Berg, B van den]]
	[[Category: Chembath, A]]		[[Category: Chembath, A]]
-	[[Category: Rutherford, J.C]]	+	[[Category: Rutherford, J C]]
		+	[[Category: Membrane protein]]

OCA at 09:47, 16 December 2015

OCA — Wed, 16 Dec 2015 09:47:06 GMT

←Older revision		Revision as of 09:47, 16 December 2015
Line 7:		Line 7:
	Description: Crystal structure of Saccharomyces cerevisiae Mep2		Description: Crystal structure of Saccharomyces cerevisiae Mep2
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Rutherford, J.C]]
	[[Category: Van Den Berg, B]]		[[Category: Van Den Berg, B]]
	[[Category: Chembath, A]]		[[Category: Chembath, A]]
		+	[[Category: Rutherford, J.C]]

OCA at 14:58, 7 February 2015

OCA — Sat, 07 Feb 2015 14:58:39 GMT

←Older revision		Revision as of 14:58, 7 February 2015
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5aex is ON HOLD	+	The entry 5aex is ON HOLD until Paper Publication

	Authors: Rutherford, J.C., Chembath, A., van den Berg, B.		Authors: Rutherford, J.C., Chembath, A., van den Berg, B.

OCA: Protected "5aex" [edit=sysop:move=sysop]

OCA — Wed, 21 Jan 2015 16:07:18 GMT

Protected "5aex" [edit=sysop:move=sysop]

←Older revision

Revision as of 16:07, 21 January 2015

OCA: New page: '''Unreleased structure''' The entry 5aex is ON HOLD Authors: Rutherford, J.C., Chembath, A., van den Berg, B. Description: Crystal structure of Saccharomyces cerevisiae Mep2 [[Categor...

OCA — Wed, 21 Jan 2015 16:07:17 GMT

New page: '''Unreleased structure''' The entry 5aex is ON HOLD Authors: Rutherford, J.C., Chembath, A., van den Berg, B. Description: Crystal structure of Saccharomyces cerevisiae Mep2 [[Categor...

New page

'''Unreleased structure'''

The entry 5aex is ON HOLD

Authors: Rutherford, J.C., Chembath, A., van den Berg, B.

Description: Crystal structure of Saccharomyces cerevisiae Mep2
[[Category: Unreleased Structures]]
[[Category: Rutherford, J.C]]
[[Category: Van Den Berg, B]]
[[Category: Chembath, A]]