5ak1 - Revision history

OCA: Redirecting to 5g56

OCA — Wed, 13 Jul 2016 02:11:29 GMT

Redirecting to 5g56

←Older revision		Revision as of 02:11, 13 July 2016
Line 1:		Line 1:
-		+	#REDIRECT [[5g56]] This PDB entry is obsolete and replaced by 5g56
-	~~==THE PENTA-MODULAR CELLULOSOMAL ARABINOXYLANASE CtXyl5A STRUCTURE AS REVEALED BY X-RAY CRYSTALLOGRAPHY==~~	+
-	~~<StructureSection load='5ak1' size='340' side='right' caption='~~[[~~5ak1]], [[Resolution\|resolution]] 2.64Å' scene=''>~~	+
-	~~== Structural highlights ==~~	+
-	~~<table><tr><td colspan='2'>[[5ak1~~]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5AK1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5AK1 FirstGlance]. <br>	+
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene></td></tr>	+
-	~~<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>~~	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ak1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ak1 OCA], [http://pdbe.org/5ak1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ak1 RCSB], [http://www.ebi.ac.uk/pdbsum/5ak1 PDBsum]</span></td></tr>	+
-	~~</table>~~	+
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	~~The cellulosome, a highly elaborate extracellular multi-enzyme complex of cellulases~~ and hemicellulases, is responsible for the degradation of plant cell walls. The xylanase CtXyl5A (Cthe_2193) is a multimodular arabinoxylanase which is one of the largest components of the Clostridium thermocellum cellulosome. The N-terminal catalytic domain of CtXyl5A, which is a member of glycoside hydrolase family 5 (GH5), is responsible for the hydrolysis of arabinoxylans. Appended after it are three noncatalytic carbohydrate-binding modules (CBMs), which belong to families 6 (CBM6), 13 (CBM13) and 62 (CBM62). In addition, CtXyl5A has a fibronectin type III-like (Fn3) module preceding the CBM62 and a type I dockerin (DOK) module following it which allows the enzyme to be integrated into the cellulosome through binding to a cohesin module of the protein scaffold CipA. Crystals of the pentamodular enzyme without the DOK module at the C-terminus, with the domain architecture CtGH5-CBM6-CBM13-Fn3-CBM62, have been obtained. The structure of this pentamodular xylanase has been determined by ~~molecular replacement to a resolution of 2.64 A using coordinates of CtGH5-CBM6, Fn3 and CBM62 from the PDB as search models.~~	+
-		+
-	Purification, crystallization and preliminary X-ray characterization of the pentamodular arabinoxylanase CtXyl5A from Clostridium thermocellum.,Bras JL, Correia MA, Romao MJ, Prates JA, Fontes CM, Najmudin S Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 Jul 1;67(Pt 7):833-6. doi:, 10.1107/S1744309111020823. Epub 2011 Jun 30. PMID:21795807<ref>PMID:21795807</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 5ak1" style="background-color:#fffaf0;"></div>~~	+
-	~~== References ==~~	+
-	~~<references/>~~	+
-	~~__TOC__~~	+
-	~~</StructureSection>~~	+
-	~~[[Category: Bras, J L.A]]~~	+
-	~~[[Category: Ferreira, L M.A]]~~	+
-	~~[[Category: Fontes, C M.G A]]~~	+
-	~~[[Category: Gilbert, H J]]~~	+
-	~~[[Category: Najmudin, S]]~~	+
-	~~[[Category: Cbm13]]~~	+
-	~~[[Category: Cbm6]]~~	+
-	~~[[Category: Cbm62]]~~	+
-	~~[[Category: Cellulosome]]~~	+
-	~~[[Category: Ctxyl5a]]~~	+
-	~~[[Category: Fn3]]~~	+
-	~~[[Category: Gh5]]~~	+
-	~~[[Category: Hydrolase]]~~	+

OCA at 03:33, 10 March 2016

OCA — Thu, 10 Mar 2016 03:33:35 GMT

←Older revision		Revision as of 03:33, 10 March 2016
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5ak1 is ~~ON HOLD until Paper~~ Publication	+	==THE PENTA-MODULAR CELLULOSOMAL ARABINOXYLANASE CtXyl5A STRUCTURE AS REVEALED BY X-RAY CRYSTALLOGRAPHY==
		+	<StructureSection load='5ak1' size='340' side='right' caption='[[5ak1]], [[Resolution\|resolution]] 2.64Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5ak1]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5AK1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5AK1 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene></td></tr>
		+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ak1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ak1 OCA], [http://pdbe.org/5ak1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ak1 RCSB], [http://www.ebi.ac.uk/pdbsum/5ak1 PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The cellulosome, a highly elaborate extracellular multi-enzyme complex of cellulases and hemicellulases, is responsible for the degradation of plant cell walls. The xylanase CtXyl5A (Cthe_2193) is a multimodular arabinoxylanase which is one of the largest components of the Clostridium thermocellum cellulosome. The N-terminal catalytic domain of CtXyl5A, which is a member of glycoside hydrolase family 5 (GH5), is responsible for the hydrolysis of arabinoxylans. Appended after it are three noncatalytic carbohydrate-binding modules (CBMs), which belong to families 6 (CBM6), 13 (CBM13) and 62 (CBM62). In addition, CtXyl5A has a fibronectin type III-like (Fn3) module preceding the CBM62 and a type I dockerin (DOK) module following it which allows the enzyme to be integrated into the cellulosome through binding to a cohesin module of the protein scaffold CipA. Crystals of the pentamodular enzyme without the DOK module at the C-terminus, with the domain architecture CtGH5-CBM6-CBM13-Fn3-CBM62, have been obtained. The structure of this pentamodular xylanase has been determined by molecular replacement to a resolution of 2.64 A using coordinates of CtGH5-CBM6, Fn3 and CBM62 from the PDB as search models.

-	~~Authors: Bras~~, ~~J.L.A~~., ~~Gilbert~~, ~~H.J.~~, ~~Ferreira~~, ~~L.M.A.~~, Fontes, C.M.G.A.~~, Najmudin, S~~.	+	Purification, crystallization and preliminary X-ray characterization of the pentamodular arabinoxylanase CtXyl5A from Clostridium thermocellum.,Bras JL, Correia MA, Romao MJ, Prates JA, Fontes CM, Najmudin S Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 Jul 1;67(Pt 7):833-6. doi:, 10.1107/S1744309111020823. Epub 2011 Jun 30. PMID:21795807<ref>PMID:21795807</ref>

-	~~Description: THE PENTA~~-~~MODULAR CELLULOSOMAL ARABINOXYLANASE CtXyl5A STRUCTURE AS REVEALED BY X~~-~~RAY CRYSTALLOGRAPHY~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
-	[[Category: Ferreira, L.M.A]]	+	<div class="pdbe-citations 5ak1" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Bras, J L.A]]
		+	[[Category: Ferreira, L M.A]]
		+	[[Category: Fontes, C M.G A]]
		+	[[Category: Gilbert, H J]]
	[[Category: Najmudin, S]]		[[Category: Najmudin, S]]
-	[[Category: ~~Bras, J.L.A~~]]	+	[[Category: Cbm13]]
-	[[Category: ~~Fontes, C.M.G.A~~]]	+	[[Category: Cbm6]]
-	[[Category: ~~Gilbert, H.J~~]]	+	[[Category: Cbm62]]
		+	[[Category: Cellulosome]]
		+	[[Category: Ctxyl5a]]
		+	[[Category: Fn3]]
		+	[[Category: Gh5]]
		+	[[Category: Hydrolase]]

OCA at 09:49, 16 December 2015

OCA — Wed, 16 Dec 2015 09:49:19 GMT

←Older revision		Revision as of 09:49, 16 December 2015
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	Description: THE PENTA-MODULAR CELLULOSOMAL ARABINOXYLANASE CtXyl5A STRUCTURE AS REVEALED BY X-RAY CRYSTALLOGRAPHY		Description: THE PENTA-MODULAR CELLULOSOMAL ARABINOXYLANASE CtXyl5A STRUCTURE AS REVEALED BY X-RAY CRYSTALLOGRAPHY
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Gilbert, H.J]]
	[[Category: Ferreira, L.M.A]]		[[Category: Ferreira, L.M.A]]
	[[Category: Najmudin, S]]		[[Category: Najmudin, S]]
	[[Category: Bras, J.L.A]]		[[Category: Bras, J.L.A]]
	[[Category: Fontes, C.M.G.A]]		[[Category: Fontes, C.M.G.A]]
		+	[[Category: Gilbert, H.J]]

OCA at 13:04, 26 March 2015

OCA — Thu, 26 Mar 2015 13:04:26 GMT

←Older revision		Revision as of 13:04, 26 March 2015
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5ak1 is ON HOLD	+	The entry 5ak1 is ON HOLD until Paper Publication

	Authors: Bras, J.L.A., Gilbert, H.J., Ferreira, L.M.A., Fontes, C.M.G.A., Najmudin, S.		Authors: Bras, J.L.A., Gilbert, H.J., Ferreira, L.M.A., Fontes, C.M.G.A., Najmudin, S.

OCA: Protected "5ak1" [edit=sysop:move=sysop]

OCA — Wed, 11 Mar 2015 11:00:57 GMT

Protected "5ak1" [edit=sysop:move=sysop]

←Older revision

Revision as of 11:00, 11 March 2015

OCA: New page: '''Unreleased structure''' The entry 5ak1 is ON HOLD Authors: Bras, J.L.A., Gilbert, H.J., Ferreira, L.M.A., Fontes, C.M.G.A., Najmudin, S. Description: THE PENTA-MODULAR CELLULOSOMAL ...

OCA — Wed, 11 Mar 2015 11:00:56 GMT

New page: '''Unreleased structure''' The entry 5ak1 is ON HOLD Authors: Bras, J.L.A., Gilbert, H.J., Ferreira, L.M.A., Fontes, C.M.G.A., Najmudin, S. Description: THE PENTA-MODULAR CELLULOSOMAL ...

New page

'''Unreleased structure'''

The entry 5ak1 is ON HOLD

Authors: Bras, J.L.A., Gilbert, H.J., Ferreira, L.M.A., Fontes, C.M.G.A., Najmudin, S.

Description: THE PENTA-MODULAR CELLULOSOMAL ARABINOXYLANASE CtXyl5A STRUCTURE AS REVEALED BY X-RAY CRYSTALLOGRAPHY
[[Category: Unreleased Structures]]
[[Category: Gilbert, H.J]]
[[Category: Ferreira, L.M.A]]
[[Category: Najmudin, S]]
[[Category: Bras, J.L.A]]
[[Category: Fontes, C.M.G.A]]