5akq - Revision history

OCA at 11:11, 10 January 2024

OCA — Wed, 10 Jan 2024 11:11:54 GMT

←Older revision		Revision as of 11:11, 10 January 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5akq]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_sp._ADP Pseudomonas sp. ADP]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5AKQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5AKQ FirstGlance]. <br>		<table><tr><td colspan='2'>[[5akq]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_sp._ADP Pseudomonas sp. ADP]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5AKQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5AKQ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.6Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5akq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5akq OCA], [https://pdbe.org/5akq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5akq RCSB], [https://www.ebi.ac.uk/pdbsum/5akq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5akq ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5akq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5akq OCA], [https://pdbe.org/5akq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5akq RCSB], [https://www.ebi.ac.uk/pdbsum/5akq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5akq ProSAT]</span></td></tr>
	</table>		</table>

OCA at 05:06, 25 May 2023

OCA — Thu, 25 May 2023 05:06:44 GMT

←Older revision		Revision as of 05:06, 25 May 2023
Line 3:		Line 3:
	<StructureSection load='5akq' size='340' side='right'caption='[[5akq]], [[Resolution\|resolution]] 2.60Å' scene=''>		<StructureSection load='5akq' size='340' side='right'caption='[[5akq]], [[Resolution\|resolution]] 2.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5akq]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Psesd Psesd~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5AKQ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5AKQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5akq]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_sp._ADP Pseudomonas sp. ADP]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5AKQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5AKQ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene~~></td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/N-isopropylammelide_isopropylaminohydrolase N-isopropylammelide isopropylaminohydrolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.99.4 3.5.99.4] </span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5akq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5akq OCA], [https://pdbe.org/5akq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5akq RCSB], [https://www.ebi.ac.uk/pdbsum/5akq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5akq ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5akq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5akq OCA], [~~http~~://pdbe.org/5akq PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5akq RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5akq PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5akq ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/ATZC_PSESD ATZC_PSESD]] Transforms N-isopropylammelide to cyanuric acid and isopropylamine.	+	[https://www.uniprot.org/uniprot/ATZC_PSESD ATZC_PSESD] Transforms N-isopropylammelide to cyanuric acid and isopropylamine.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 24:		Line 23:
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~N-isopropylammelide isopropylaminohydrolase]]~~	+	[[Category: Pseudomonas sp. ADP]]
-	~~[[Category: Psesd~~]]	+	[[Category: Balotra S]]
-	[[Category: Balotra, S]]	+	[[Category: Briggs LJ]]
-	[[Category: Briggs~~, L J~~]]	+	[[Category: Newman J]]
-	[[Category: Newman, J]]	+	[[Category: Peat TS]]
-	[[Category: Peat~~, T S~~]]	+	[[Category: Scott C]]
-	[[Category: Scott, C]]	+	[[Category: Warden AC]]
-	[[Category: Warden~~, A C]]~~	+
-	~~[[Category: Atrazine degradation]]~~	+
-	~~[[Category: Enzyme evolution]]~~	+
-	~~[[Category: Hydrolase~~]]	+

OCA at 07:14, 3 July 2019

OCA — Wed, 03 Jul 2019 07:14:27 GMT

←Older revision		Revision as of 07:14, 3 July 2019
Line 1:		Line 1:
		+
	==X-ray structure and mutagenesis studies of the N-isopropylammelide isopropylaminohydrolase, AtzC==		==X-ray structure and mutagenesis studies of the N-isopropylammelide isopropylaminohydrolase, AtzC==
-	<StructureSection load='5akq' size='340' side='right' caption='[[5akq]], [[Resolution\|resolution]] 2.60Å' scene=''>	+	<StructureSection load='5akq' size='340' side='right'caption='[[5akq]], [[Resolution\|resolution]] 2.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5akq]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5AKQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5AKQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5akq]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Psesd Psesd]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5AKQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5AKQ FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/N-isopropylammelide_isopropylaminohydrolase N-isopropylammelide isopropylaminohydrolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.99.4 3.5.99.4] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/N-isopropylammelide_isopropylaminohydrolase N-isopropylammelide isopropylaminohydrolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.99.4 3.5.99.4] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5akq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5akq OCA], [http://pdbe.org/5akq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5akq RCSB], [http://www.ebi.ac.uk/pdbsum/5akq PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5akq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5akq OCA], [http://pdbe.org/5akq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5akq RCSB], [http://www.ebi.ac.uk/pdbsum/5akq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5akq ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 22:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: N-isopropylammelide isopropylaminohydrolase]]		[[Category: N-isopropylammelide isopropylaminohydrolase]]
		+	[[Category: Psesd]]
	[[Category: Balotra, S]]		[[Category: Balotra, S]]
	[[Category: Briggs, L J]]		[[Category: Briggs, L J]]

OCA at 06:19, 30 September 2015

OCA — Wed, 30 Sep 2015 06:19:14 GMT

←Older revision		Revision as of 06:19, 30 September 2015
Line 5:		Line 5:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/N-isopropylammelide_isopropylaminohydrolase N-isopropylammelide isopropylaminohydrolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.99.4 3.5.99.4] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/N-isopropylammelide_isopropylaminohydrolase N-isopropylammelide isopropylaminohydrolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.99.4 3.5.99.4] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5akq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5akq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=5akq RCSB], [http://www.ebi.ac.uk/pdbsum/5akq PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5akq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5akq OCA], [http://pdbe.org/5akq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5akq RCSB], [http://www.ebi.ac.uk/pdbsum/5akq PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/ATZC_PSESD ATZC_PSESD]] Transforms N-isopropylammelide to cyanuric acid and isopropylamine.		[[http://www.uniprot.org/uniprot/ATZC_PSESD ATZC_PSESD]] Transforms N-isopropylammelide to cyanuric acid and isopropylamine.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The N-isopropylammelide isopropylaminohydrolase from Pseudomonas sp. strain ADP, AtzC, provides the third hydrolytic step in the mineralization of s-triazine herbicides, such as atrazine. We obtained the X-ray crystal structure of AtzC at 1.84 A with a weak inhibitor bound in the active site and then used a combination of in silico docking and site-directed mutagenesis to understand the interactions between AtzC and its substrate, isopropylammelide. The substitution of an active site histidine residue (His249) for an alanine abolished the enzyme's catalytic activity. We propose a plausible catalytic mechanism, consistent with the biochemical and crystallographic data obtained that is similar to that found in carbonic anhydrase and other members of subtype III of the amidohydrolase family.
		+
		+	X-Ray Structure and Mutagenesis Studies of the N-Isopropylammelide Isopropylaminohydrolase, AtzC.,Balotra S, Warden AC, Newman J, Briggs LJ, Scott C, Peat TS PLoS One. 2015 Sep 21;10(9):e0137700. doi: 10.1371/journal.pone.0137700., eCollection 2015. PMID:26390431<ref>PMID:26390431</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5akq" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 12:19, 18 March 2015

OCA — Wed, 18 Mar 2015 12:19:42 GMT

←Older revision		Revision as of 12:19, 18 March 2015
Line 1:		Line 1:
-	'''~~Unreleased structure~~'''	+	==X-ray structure and mutagenesis studies of the N-isopropylammelide isopropylaminohydrolase, AtzC==
-		+	<StructureSection load='5akq' size='340' side='right' caption='[[5akq]], [[Resolution\|resolution]] 2.60Å' scene=''>
-	~~The entry~~ 5akq is ~~ON HOLD~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5akq]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5AKQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5AKQ FirstGlance]. <br>
-	~~Authors~~: ~~Balotra, S~~.~~, Warden, A~~.C.~~, Newman, J~~.~~, Briggs, L~~.J.~~, Scott, C~~.~~, Peat, T~~.S.	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/N-isopropylammelide_isopropylaminohydrolase N-isopropylammelide isopropylaminohydrolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.99.4 3.5.99.4] </span></td></tr>
-	~~Description~~: X-~~ray structure and mutagenesis studies of the~~ N-isopropylammelide isopropylaminohydrolase, ~~AtzC~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5akq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5akq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=5akq RCSB], [http://www.ebi.ac.uk/pdbsum/5akq PDBsum]</span></td></tr>
-	[~~[Category~~: ~~Unreleased Structures]~~]	+	</table>
-	[~~[Category~~: ~~Scott, C~~]]	+	== Function ==
-	[~~[Category~~: ~~Briggs, L~~.J]]	+	[[http://www.uniprot.org/uniprot/ATZC_PSESD ATZC_PSESD]] Transforms N-isopropylammelide to cyanuric acid and isopropylamine.
-	[~~[Category~~: ~~Peat~~, T.S]]	+	__TOC__
-	[[~~Category~~: ~~Newman, J~~]]	+	</StructureSection>
-	[[Category: ~~Warden, A.C~~]]	+	[[Category: N-isopropylammelide isopropylaminohydrolase]]
	[[Category: Balotra, S]]		[[Category: Balotra, S]]
		+	[[Category: Briggs, L J]]
		+	[[Category: Newman, J]]
		+	[[Category: Peat, T S]]
		+	[[Category: Scott, C]]
		+	[[Category: Warden, A C]]
		+	[[Category: Atrazine degradation]]
		+	[[Category: Enzyme evolution]]
		+	[[Category: Hydrolase]]

OCA: Protected "5akq" [edit=sysop:move=sysop]

OCA — Wed, 11 Mar 2015 11:03:16 GMT

Protected "5akq" [edit=sysop:move=sysop]

←Older revision

Revision as of 11:03, 11 March 2015

OCA: New page: '''Unreleased structure''' The entry 5akq is ON HOLD Authors: Balotra, S., Warden, A.C., Newman, J., Briggs, L.J., Scott, C., Peat, T.S. Description: X-ray structure and mutagenesis st...

OCA — Wed, 11 Mar 2015 11:03:15 GMT

New page: '''Unreleased structure''' The entry 5akq is ON HOLD Authors: Balotra, S., Warden, A.C., Newman, J., Briggs, L.J., Scott, C., Peat, T.S. Description: X-ray structure and mutagenesis st...

New page

'''Unreleased structure'''

The entry 5akq is ON HOLD

Authors: Balotra, S., Warden, A.C., Newman, J., Briggs, L.J., Scott, C., Peat, T.S.

Description: X-ray structure and mutagenesis studies of the N-isopropylammelide isopropylaminohydrolase, AtzC
[[Category: Unreleased Structures]]
[[Category: Scott, C]]
[[Category: Briggs, L.J]]
[[Category: Peat, T.S]]
[[Category: Newman, J]]
[[Category: Warden, A.C]]
[[Category: Balotra, S]]