5aun - Revision history

OCA at 15:48, 8 November 2023

OCA — Wed, 08 Nov 2023 15:48:29 GMT

←Older revision		Revision as of 15:48, 8 November 2023
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5aun]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermococcus_kodakarensis_KOD1 Thermococcus kodakarensis KOD1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5AUN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5AUN FirstGlance]. <br>		<table><tr><td colspan='2'>[[5aun]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermococcus_kodakarensis_KOD1 Thermococcus kodakarensis KOD1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5AUN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5AUN FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>, <scene name='pdbligand=URE:UREA'>URE</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.63Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>, <scene name='pdbligand=URE:UREA'>URE</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5aun FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5aun OCA], [https://pdbe.org/5aun PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5aun RCSB], [https://www.ebi.ac.uk/pdbsum/5aun PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5aun ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5aun FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5aun OCA], [https://pdbe.org/5aun PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5aun RCSB], [https://www.ebi.ac.uk/pdbsum/5aun PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5aun ProSAT]</span></td></tr>
	</table>		</table>

OCA at 06:26, 31 May 2023

OCA — Wed, 31 May 2023 06:26:25 GMT

←Older revision		Revision as of 06:26, 31 May 2023
Line 3:		Line 3:
	<StructureSection load='5aun' size='340' side='right'caption='[[5aun]], [[Resolution\|resolution]] 1.63Å' scene=''>		<StructureSection load='5aun' size='340' side='right'caption='[[5aun]], [[Resolution\|resolution]] 1.63Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5aun]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Pyrococcus_kodakaraensis_(strain_kod1) Pyrococcus kodakaraensis (strain kod1)~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5AUN OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5AUN FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5aun]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermococcus_kodakarensis_KOD1 Thermococcus kodakarensis KOD1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5AUN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5AUN FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>, <scene name='pdbligand=URE:UREA'>URE</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>, <scene name='pdbligand=URE:UREA'>URE</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3a43\|3a43]], [[3vx3\|3vx3]], [[5auo\|5auo]], [[5aup\|5aup]], [[5auq\|5auq]]</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5aun FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5aun OCA], [https://pdbe.org/5aun PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5aun RCSB], [https://www.ebi.ac.uk/pdbsum/5aun PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5aun ProSAT]</span></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">hypA, TK2008 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=69014 Pyrococcus kodakaraensis (strain KOD1)]), TK2007 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=69014 Pyrococcus kodakaraensis (strain KOD1)])</td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5aun FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5aun OCA], [~~http~~://pdbe.org/5aun PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5aun RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5aun PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5aun ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/HYPA_THEKO HYPA_THEKO]] Probably plays a role in a hydrogenase nickel cofactor insertion step (By similarity).	+	[https://www.uniprot.org/uniprot/HYPA_THEKO HYPA_THEKO] Probably plays a role in a hydrogenase nickel cofactor insertion step (By similarity).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 20:		Line 18:
	</div>		</div>
	<div class="pdbe-citations 5aun" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5aun" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[HypA%2C HypB%2C HypC%2C HypD%2C HypE and HypF 3D structures\|HypA%2C HypB%2C HypC%2C HypD%2C HypE and HypF 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 25:		Line 26:
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Kawashima, T]]	+	[[Category: Thermococcus kodakarensis KOD1]]
-	[[Category: Miki, K]]	+	[[Category: Kawashima T]]
-	[[Category: Nishitani, Y]]	+	[[Category: Miki K]]
-	[[Category: Watanabe, S]]	+	[[Category: Nishitani Y]]
-	~~[[Category: Metal binding protein-hydrolase complex]]~~	+	[[Category: Watanabe S]]
-	~~[[Category: Metallochaperone]]~~	+
-	~~[[Category: Protein complex~~]]	+

OCA at 07:21, 3 July 2019

OCA — Wed, 03 Jul 2019 07:21:24 GMT

←Older revision		Revision as of 07:21, 3 July 2019
Line 1:		Line 1:

	==Crystal structure of the HypAB-Ni complex==		==Crystal structure of the HypAB-Ni complex==
-	<StructureSection load='5aun' size='340' side='right' caption='[[5aun]], [[Resolution\|resolution]] 1.63Å' scene=''>	+	<StructureSection load='5aun' size='340' side='right'caption='[[5aun]], [[Resolution\|resolution]] 1.63Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5aun]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5AUN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5AUN FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5aun]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pyrococcus_kodakaraensis_(strain_kod1) Pyrococcus kodakaraensis (strain kod1)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5AUN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5AUN FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>, <scene name='pdbligand=URE:UREA'>URE</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>, <scene name='pdbligand=URE:UREA'>URE</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3a43\|3a43]], [[3vx3\|3vx3]], [[5auo\|5auo]], [[5aup\|5aup]], [[5auq\|5auq]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3a43\|3a43]], [[3vx3\|3vx3]], [[5auo\|5auo]], [[5aup\|5aup]], [[5auq\|5auq]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">hypA, TK2008 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=69014 Pyrococcus kodakaraensis (strain KOD1)]), TK2007 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=69014 Pyrococcus kodakaraensis (strain KOD1)])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5aun FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5aun OCA], [http://pdbe.org/5aun PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5aun RCSB], [http://www.ebi.ac.uk/pdbsum/5aun PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5aun ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5aun FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5aun OCA], [http://pdbe.org/5aun PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5aun RCSB], [http://www.ebi.ac.uk/pdbsum/5aun PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5aun ProSAT]</span></td></tr>
	</table>		</table>
Line 23:		Line 24:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Kawashima, T]]		[[Category: Kawashima, T]]
	[[Category: Miki, K]]		[[Category: Miki, K]]
	[[Category: Nishitani, Y]]		[[Category: Nishitani, Y]]
	[[Category: Watanabe, S]]		[[Category: Watanabe, S]]
		+	[[Category: Metal binding protein-hydrolase complex]]
	[[Category: Metallochaperone]]		[[Category: Metallochaperone]]
	[[Category: Protein complex]]		[[Category: Protein complex]]

OCA at 12:08, 12 April 2017

OCA — Wed, 12 Apr 2017 12:08:47 GMT

←Older revision		Revision as of 12:08, 12 April 2017
Line 1:		Line 1:
		+
	==Crystal structure of the HypAB-Ni complex==		==Crystal structure of the HypAB-Ni complex==
	<StructureSection load='5aun' size='340' side='right' caption='[[5aun]], [[Resolution\|resolution]] 1.63Å' scene=''>		<StructureSection load='5aun' size='340' side='right' caption='[[5aun]], [[Resolution\|resolution]] 1.63Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>, <scene name='pdbligand=URE:UREA'>URE</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>, <scene name='pdbligand=URE:UREA'>URE</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3a43\|3a43]], [[3vx3\|3vx3]], [[5auo\|5auo]], [[5aup\|5aup]], [[5auq\|5auq]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3a43\|3a43]], [[3vx3\|3vx3]], [[5auo\|5auo]], [[5aup\|5aup]], [[5auq\|5auq]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5aun FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5aun OCA], [http://www.rcsb.org/pdb/explore.do?structureId=5aun RCSB], [http://www.ebi.ac.uk/pdbsum/5aun PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5aun FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5aun OCA], [http://pdbe.org/5aun PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5aun RCSB], [http://www.ebi.ac.uk/pdbsum/5aun PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5aun ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 17:		Line 18:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 5aun" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 12:50, 24 June 2015

OCA — Wed, 24 Jun 2015 12:50:03 GMT

←Older revision		Revision as of 12:50, 24 June 2015
Line 1:		Line 1:
-	'''~~Unreleased~~ structure'''	+	==Crystal structure of the HypAB-Ni complex==
		+	<StructureSection load='5aun' size='340' side='right' caption='[[5aun]], [[Resolution\|resolution]] 1.63Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5aun]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5AUN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5AUN FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>, <scene name='pdbligand=URE:UREA'>URE</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3a43\|3a43]], [[3vx3\|3vx3]], [[5auo\|5auo]], [[5aup\|5aup]], [[5auq\|5auq]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5aun FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5aun OCA], [http://www.rcsb.org/pdb/explore.do?structureId=5aun RCSB], [http://www.ebi.ac.uk/pdbsum/5aun PDBsum]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/HYPA_THEKO HYPA_THEKO]] Probably plays a role in a hydrogenase nickel cofactor insertion step (By similarity).
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The Ni atom at the catalytic center of [NiFe] hydrogenases is incorporated by a Ni-metallochaperone, HypA, and a GTPase/ATPase, HypB. We report the crystal structures of the transient complex formed between HypA and ATPase-type HypB (HypBAT) with Ni ions. Transient association between HypA and HypBAT is controlled by the ATP hydrolysis cycle of HypBAT, which is accelerated by HypA. Only the ATP-bound form of HypBAT can interact with HypA and induces drastic conformational changes of HypA. Consequently, upon complex formation, a conserved His residue of HypA comes close to the N-terminal conserved motif of HypA and forms a Ni-binding site, to which a Ni ion is bound with a nearly square-planar geometry. The Ni binding site in the HypABAT complex has a nanomolar affinity (Kd = 7 nM), which is in contrast to the micromolar affinity (Kd = 4 microM) observed with the isolated HypA. The ATP hydrolysis and Ni binding cause conformational changes of HypBAT, affecting its association with HypA. These findings indicate that HypA and HypBAT constitute an ATP-dependent Ni acquisition cycle for [NiFe]-hydrogenase maturation, wherein HypBAT functions as a metallochaperone enhancer and considerably increases the Ni-binding affinity of HypA.

-	~~The entry 5aun is ON HOLD~~	+	Structural basis of a Ni acquisition cycle for [NiFe] hydrogenase by Ni-metallochaperone HypA and its enhancer.,Watanabe S, Kawashima T, Nishitani Y, Kanai T, Wada T, Inaba K, Atomi H, Imanaka T, Miki K Proc Natl Acad Sci U S A. 2015 Jun 8. pii: 201503102. PMID:26056269<ref>PMID:26056269</ref>

-	~~Authors: Watanabe~~, S.~~, Kawashima, T., Nishitani, Y., Miki, K~~.	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~Description: Crystal structure of the HypAB-Ni complex~~	+	== References ==
-	~~[[Category: Unreleased Structures]]~~	+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Kawashima, T]]		[[Category: Kawashima, T]]
	[[Category: Miki, K]]		[[Category: Miki, K]]
		+	[[Category: Nishitani, Y]]
	[[Category: Watanabe, S]]		[[Category: Watanabe, S]]
-	[[Category: ~~Nishitani, Y~~]]	+	[[Category: Metallochaperone]]
		+	[[Category: Protein complex]]

OCA: Protected "5aun" [edit=sysop:move=sysop]

OCA — Wed, 03 Jun 2015 11:36:52 GMT

Protected "5aun" [edit=sysop:move=sysop]

←Older revision

Revision as of 11:36, 3 June 2015

OCA: New page: '''Unreleased structure''' The entry 5aun is ON HOLD Authors: Watanabe, S., Kawashima, T., Nishitani, Y., Miki, K. Description: Crystal structure of the HypAB-Ni complex [[Category: Un...

OCA — Wed, 03 Jun 2015 11:36:51 GMT

New page: '''Unreleased structure''' The entry 5aun is ON HOLD Authors: Watanabe, S., Kawashima, T., Nishitani, Y., Miki, K. Description: Crystal structure of the HypAB-Ni complex [[Category: Un...

New page

'''Unreleased structure'''

The entry 5aun is ON HOLD

Authors: Watanabe, S., Kawashima, T., Nishitani, Y., Miki, K.

Description: Crystal structure of the HypAB-Ni complex
[[Category: Unreleased Structures]]
[[Category: Kawashima, T]]
[[Category: Miki, K]]
[[Category: Watanabe, S]]
[[Category: Nishitani, Y]]