5ayi - Revision history

OCA at 15:51, 8 November 2023

OCA — Wed, 08 Nov 2023 15:51:23 GMT

←Older revision		Revision as of 15:51, 8 November 2023
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5ayi]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Metagenomes Metagenomes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5AYI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5AYI FirstGlance]. <br>		<table><tr><td colspan='2'>[[5ayi]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Metagenomes Metagenomes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5AYI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5AYI FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NHE:2-[N-CYCLOHEXYLAMINO]ETHANE+SULFONIC+ACID'>NHE</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.85Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NHE:2-[N-CYCLOHEXYLAMINO]ETHANE+SULFONIC+ACID'>NHE</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ayi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ayi OCA], [https://pdbe.org/5ayi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ayi RCSB], [https://www.ebi.ac.uk/pdbsum/5ayi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ayi ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ayi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ayi OCA], [https://pdbe.org/5ayi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ayi RCSB], [https://www.ebi.ac.uk/pdbsum/5ayi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ayi ProSAT]</span></td></tr>
	</table>		</table>

OCA at 06:31, 31 May 2023

OCA — Wed, 31 May 2023 06:31:23 GMT

←Older revision		Revision as of 06:31, 31 May 2023
Line 3:		Line 3:
	<StructureSection load='5ayi' size='340' side='right'caption='[[5ayi]], [[Resolution\|resolution]] 1.85Å' scene=''>		<StructureSection load='5ayi' size='340' side='right'caption='[[5ayi]], [[Resolution\|resolution]] 1.85Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5ayi]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Metagenomes Metagenomes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5AYI OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5AYI FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5ayi]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Metagenomes Metagenomes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5AYI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5AYI FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NHE:2-[N-CYCLOHEXYLAMINO]ETHANE+SULFONIC+ACID'>NHE</scene~~></td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NHE:2-[N-CYCLOHEXYLAMINO]ETHANE+SULFONIC+ACID'>NHE</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5ayb\|5ayb]]</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ayi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ayi OCA], [https://pdbe.org/5ayi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ayi RCSB], [https://www.ebi.ac.uk/pdbsum/5ayi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ayi ProSAT]</span></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-glucosidase Beta-glucosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.21 3.2.1.21] </span></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5ayi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ayi OCA], [~~http~~://pdbe.org/5ayi PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5ayi RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5ayi PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5ayi ProSAT]</span></td></tr>	+
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 25:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Beta-glucosidase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Metagenomes]]		[[Category: Metagenomes]]
-	[[Category: Arakawa, T]]	+	[[Category: Arakawa T]]
-	[[Category: Fushinobu, S]]	+	[[Category: Fushinobu S]]
-	[[Category: Jo, T]]	+	[[Category: Jo T]]
-	[[Category: Manninen~~, J A~~]]	+	[[Category: Manninen JA]]
-	[[Category: Matsuzawa, T]]	+	[[Category: Matsuzawa T]]
-	[[Category: Uchiyama, T]]	+	[[Category: Uchiyama T]]
-	[[Category: Yaoi, K]]	+	[[Category: Yaoi K]]
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Tim barrel~~]]	+

OCA at 09:56, 26 February 2020

OCA — Wed, 26 Feb 2020 09:56:24 GMT

←Older revision		Revision as of 09:56, 26 February 2020
Line 1:		Line 1:

	==Crystal structure of GH1 Beta-glucosidase TD2F2 N223Q mutant==		==Crystal structure of GH1 Beta-glucosidase TD2F2 N223Q mutant==
-	<StructureSection load='5ayi' size='340' side='right' caption='[[5ayi]], [[Resolution\|resolution]] 1.85Å' scene=''>	+	<StructureSection load='5ayi' size='340' side='right'caption='[[5ayi]], [[Resolution\|resolution]] 1.85Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5ayi]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5AYI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5AYI FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5ayi]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Metagenomes Metagenomes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5AYI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5AYI FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NHE:2-[N-CYCLOHEXYLAMINO]ETHANE+SULFONIC+ACID'>NHE</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NHE:2-[N-CYCLOHEXYLAMINO]ETHANE+SULFONIC+ACID'>NHE</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5ayb\|5ayb]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5ayb\|5ayb]]</td></tr>
Line 18:		Line 18:
	</div>		</div>
	<div class="pdbe-citations 5ayi" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5ayi" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Beta-glucosidase 3D structures\|Beta-glucosidase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 23:		Line 26:
	</StructureSection>		</StructureSection>
	[[Category: Beta-glucosidase]]		[[Category: Beta-glucosidase]]
		+	[[Category: Large Structures]]
		+	[[Category: Metagenomes]]
	[[Category: Arakawa, T]]		[[Category: Arakawa, T]]
	[[Category: Fushinobu, S]]		[[Category: Fushinobu, S]]

OCA at 18:29, 12 July 2016

OCA — Tue, 12 Jul 2016 18:29:23 GMT

←Older revision		Revision as of 18:29, 12 July 2016
Line 7:		Line 7:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5ayb\|5ayb]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5ayb\|5ayb]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-glucosidase Beta-glucosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.21 3.2.1.21] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-glucosidase Beta-glucosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.21 3.2.1.21] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ayi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ayi OCA], [http://pdbe.org/5ayi PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ayi RCSB], [http://www.ebi.ac.uk/pdbsum/5ayi PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ayi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ayi OCA], [http://pdbe.org/5ayi PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ayi RCSB], [http://www.ebi.ac.uk/pdbsum/5ayi PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ayi ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 16:30, 10 May 2016

OCA — Tue, 10 May 2016 16:30:28 GMT

←Older revision		Revision as of 16:30, 10 May 2016
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5ayi is ~~ON HOLD until Aug~~ 21 ~~2017~~	+	==Crystal structure of GH1 Beta-glucosidase TD2F2 N223Q mutant==
		+	<StructureSection load='5ayi' size='340' side='right' caption='[[5ayi]], [[Resolution\|resolution]] 1.85Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5ayi]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5AYI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5AYI FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NHE:2-[N-CYCLOHEXYLAMINO]ETHANE+SULFONIC+ACID'>NHE</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5ayb\|5ayb]]</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-glucosidase Beta-glucosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.21 3.2.1.21] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ayi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ayi OCA], [http://pdbe.org/5ayi PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ayi RCSB], [http://www.ebi.ac.uk/pdbsum/5ayi PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	beta-Glucosidase Td2F2 isolated from a compost metagenome has high glucose tolerance and transglycosylation activity. In this study, we determined the high-resolution crystal structure of Td2F2. It has a unique structure at the -1 subsite that is important for substrate specificity but not for glucose tolerance. To elucidate the mechanism(s) of glucose tolerance, we isolated a glucose-sensitive Td2F2 mutant using random mutagenesis. In this mutant, Asn223 residue located between subsites +1 and +2 was mutated. The Asn223 mutation resulted in reduced glucose tolerance and transglycosylation activity, and drastically changed substrate specificity. These results indicate that the structure between subsites +1 and +2 is critical for the glucose tolerance and substrate specificity of Td2F2. Our findings shed light on the glucose tolerance and transglycosylation activity mechanisms of glycoside hydrolase family 1 beta-glucosidases. This article is protected by copyright. All rights reserved.

-	~~Authors: Jo~~, ~~T., Manninen, J.A~~., Matsuzawa, T., Uchiyama, T., ~~Yaoi, K.~~, Arakawa, T., Fushinobu, S.	+	Crystal structure and identification of a key amino acid for glucose tolerance, substrate specificity and transglycosylation activity of metagenomic beta-glucosidase Td2F2.,Matsuzawa T, Jo T, Uchiyama T, Manninen JA, Arakawa T, Miyazaki K, Fushinobu S, Yaoi K FEBS J. 2016 Apr 19. doi: 10.1111/febs.13743. PMID:27092463<ref>PMID:27092463</ref>

-	~~Description: Crystal structure~~ of ~~GH1 Beta-glucosidase TD2F2 N223Q mutant~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category~~: ~~Unreleased Structures]]~~	+	</div>
-	[[Category: ~~Uchiyama, T~~]]	+	<div class="pdbe-citations 5ayi" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Beta-glucosidase]]
	[[Category: Arakawa, T]]		[[Category: Arakawa, T]]
	[[Category: Fushinobu, S]]		[[Category: Fushinobu, S]]
		+	[[Category: Jo, T]]
		+	[[Category: Manninen, J A]]
	[[Category: Matsuzawa, T]]		[[Category: Matsuzawa, T]]
		+	[[Category: Uchiyama, T]]
	[[Category: Yaoi, K]]		[[Category: Yaoi, K]]
-	[[Category: ~~Manninen, J.A~~]]	+	[[Category: Hydrolase]]
-	[[Category: ~~Jo, T~~]]	+	[[Category: Tim barrel]]

OCA at 10:00, 16 December 2015

OCA — Wed, 16 Dec 2015 10:00:25 GMT

←Older revision		Revision as of 10:00, 16 December 2015
Line 7:		Line 7:
	Description: Crystal structure of GH1 Beta-glucosidase TD2F2 N223Q mutant		Description: Crystal structure of GH1 Beta-glucosidase TD2F2 N223Q mutant
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Fushinobu, S]]
-	[[Category: Matsuzawa, T]]
-	[[Category: Manninen, J.A]]
	[[Category: Uchiyama, T]]		[[Category: Uchiyama, T]]
	[[Category: Arakawa, T]]		[[Category: Arakawa, T]]
		+	[[Category: Fushinobu, S]]
		+	[[Category: Matsuzawa, T]]
	[[Category: Yaoi, K]]		[[Category: Yaoi, K]]
		+	[[Category: Manninen, J.A]]
	[[Category: Jo, T]]		[[Category: Jo, T]]

OCA at 10:01, 2 September 2015

OCA — Wed, 02 Sep 2015 10:01:00 GMT

←Older revision		Revision as of 10:01, 2 September 2015
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5ayi is ON HOLD	+	The entry 5ayi is ON HOLD until Aug 21 2017

-	Authors: Jo, T., Manninen, J.A., Matsuzawa T., Uchiyama, T., Yaoi, K., Arakawa, T., Fushinobu, S.	+	Authors: Jo, T., Manninen, J.A., Matsuzawa, T., Uchiyama, T., Yaoi, K., Arakawa, T., Fushinobu, S.

-	Description: ~~CRYSTAL STRUCTURE OF~~ GH1 ~~BETA~~-~~GLUCOSIDASE~~ TD2F2 N223Q ~~MUTANT~~	+	Description: Crystal structure of GH1 Beta-glucosidase TD2F2 N223Q mutant
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: Fushinobu, S]]		[[Category: Fushinobu, S]]
-	[[Category: Matsuzawa T]]	+	[[Category: Matsuzawa, T]]
	[[Category: Manninen, J.A]]		[[Category: Manninen, J.A]]
	[[Category: Uchiyama, T]]		[[Category: Uchiyama, T]]

OCA: Protected "5ayi" [edit=sysop:move=sysop]

OCA — Wed, 26 Aug 2015 11:12:42 GMT

Protected "5ayi" [edit=sysop:move=sysop]

←Older revision

Revision as of 11:12, 26 August 2015

OCA: New page: '''Unreleased structure''' The entry 5ayi is ON HOLD Authors: Jo, T., Manninen, J.A., Matsuzawa T., Uchiyama, T., Yaoi, K., Arakawa, T., Fushinobu, S. Description: CRYSTAL STRUCTURE OF...

OCA — Wed, 26 Aug 2015 11:12:40 GMT

New page: '''Unreleased structure''' The entry 5ayi is ON HOLD Authors: Jo, T., Manninen, J.A., Matsuzawa T., Uchiyama, T., Yaoi, K., Arakawa, T., Fushinobu, S. Description: CRYSTAL STRUCTURE OF...

New page

'''Unreleased structure'''

The entry 5ayi is ON HOLD

Authors: Jo, T., Manninen, J.A., Matsuzawa T., Uchiyama, T., Yaoi, K., Arakawa, T., Fushinobu, S.

Description: CRYSTAL STRUCTURE OF GH1 BETA-GLUCOSIDASE TD2F2 N223Q MUTANT
[[Category: Unreleased Structures]]
[[Category: Fushinobu, S]]
[[Category: Matsuzawa T]]
[[Category: Manninen, J.A]]
[[Category: Uchiyama, T]]
[[Category: Arakawa, T]]
[[Category: Yaoi, K]]
[[Category: Jo, T]]