5azh - Revision history

OCA at 09:06, 20 March 2024

OCA — Wed, 20 Mar 2024 09:06:43 GMT

←Older revision		Revision as of 09:06, 20 March 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5azh]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Caenorhabditis_elegans Caenorhabditis elegans] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5AZH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5AZH FirstGlance]. <br>		<table><tr><td colspan='2'>[[5azh]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Caenorhabditis_elegans Caenorhabditis elegans] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5AZH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5AZH FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.3Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5azh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5azh OCA], [https://pdbe.org/5azh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5azh RCSB], [https://www.ebi.ac.uk/pdbsum/5azh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5azh ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5azh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5azh OCA], [https://pdbe.org/5azh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5azh RCSB], [https://www.ebi.ac.uk/pdbsum/5azh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5azh ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/LGG2_CAEEL LGG2_CAEEL] Ubiquitin-like modifier involved in autophagy and essential for dauer development and life-span extension.<ref>PMID:20523114</ref>		[https://www.uniprot.org/uniprot/LGG2_CAEEL LGG2_CAEEL] Ubiquitin-like modifier involved in autophagy and essential for dauer development and life-span extension.<ref>PMID:20523114</ref>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Multicellular organisms have multiple homologs of the yeast ATG8 gene, but the differential roles of these homologs in autophagy during development remain largely unknown. Here we investigated structure/function relationships in the two C. elegans Atg8 homologs, LGG-1 and LGG-2. lgg-1 is essential for degradation of protein aggregates, while lgg-2 has cargo-specific and developmental-stage-specific roles in aggregate degradation. Crystallography revealed that the N-terminal tails of LGG-1 and LGG-2 adopt the closed and open form, respectively. LGG-1 and LGG-2 interact differentially with autophagy substrates and Atg proteins, many of which carry a LIR motif. LGG-1 and LGG-2 have structurally distinct substrate binding pockets that prefer different residues in the interacting LIR motif, thus influencing binding specificity. Lipidated LGG-1 and LGG-2 possess distinct membrane tethering and fusion activities, which may result from the N-terminal differences. Our study reveals the differential function of two ATG8 homologs in autophagy during C. elegans development.
-
-	Structural Basis of the Differential Function of the Two C. elegans Atg8 Homologs, LGG-1 and LGG-2, in Autophagy.,Wu F, Watanabe Y, Guo XY, Qi X, Wang P, Zhao HY, Wang Z, Fujioka Y, Zhang H, Ren JQ, Fang TC, Shen YX, Feng W, Hu JJ, Noda NN, Zhang H Mol Cell. 2015 Dec 17;60(6):914-29. doi: 10.1016/j.molcel.2015.11.019. PMID:26687600<ref>PMID:26687600</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 5azh" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 06:32, 31 May 2023

OCA — Wed, 31 May 2023 06:32:42 GMT

←Older revision		Revision as of 06:32, 31 May 2023
Line 3:		Line 3:
	<StructureSection load='5azh' size='340' side='right'caption='[[5azh]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='5azh' size='340' side='right'caption='[[5azh]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5azh]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Synthetic_construct_sequences~~ Synthetic construct ~~sequences~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5AZH OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5AZH FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5azh]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Caenorhabditis_elegans Caenorhabditis elegans] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5AZH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5AZH FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5azf\|5azf]], [[5azg\|5azg]]</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5azh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5azh OCA], [https://pdbe.org/5azh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5azh RCSB], [https://www.ebi.ac.uk/pdbsum/5azh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5azh ProSAT]</span></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">lgg-2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=32630 SYNTHETIC CONSTRUCT sequences])</td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5azh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5azh OCA], [~~http~~://pdbe.org/5azh PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5azh RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5azh PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5azh ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/LGG2_CAEEL LGG2_CAEEL] Ubiquitin-like modifier involved in autophagy and essential for dauer development and life-span extension.<ref>PMID:20523114</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 22:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Caenorhabditis elegans]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Synthetic construct ~~sequences~~]]	+	[[Category: Synthetic construct]]
-	[[Category: Fujioka, Y]]	+	[[Category: Fujioka Y]]
-	[[Category: Noda~~, N N~~]]	+	[[Category: Noda NN]]
-	[[Category: Watanabe, Y]]	+	[[Category: Watanabe Y]]
-	~~[[Category: Autophagy]]~~	+
-	~~[[Category: Protein binding]]~~	+
-	~~[[Category: Ubiquitin-like~~]]	+

OCA at 08:51, 1 January 2020

OCA — Wed, 01 Jan 2020 08:51:02 GMT

←Older revision		Revision as of 08:51, 1 January 2020
Line 1:		Line 1:
		+
	==Crystal structure of LGG-2 fused with an EEEWEEL peptide==		==Crystal structure of LGG-2 fused with an EEEWEEL peptide==
-	<StructureSection load='5azh' size='340' side='right' caption='[[5azh]], [[Resolution\|resolution]] 2.30Å' scene=''>	+	<StructureSection load='5azh' size='340' side='right'caption='[[5azh]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5azh]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5AZH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5AZH FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5azh]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Synthetic_construct_sequences Synthetic construct sequences]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5AZH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5AZH FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5azf\|5azf]], [[5azg\|5azg]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5azf\|5azf]], [[5azg\|5azg]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5azh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5azh OCA], [http://pdbe.org/5azh PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5azh RCSB], [http://www.ebi.ac.uk/pdbsum/5azh PDBsum]</span></td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">lgg-2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=32630 SYNTHETIC CONSTRUCT sequences])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5azh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5azh OCA], [http://pdbe.org/5azh PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5azh RCSB], [http://www.ebi.ac.uk/pdbsum/5azh PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5azh ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Multicellular organisms have multiple homologs of the yeast ATG8 gene, but the differential roles of these homologs in autophagy during development remain largely unknown. Here we investigated structure/function relationships in the two C. elegans Atg8 homologs, LGG-1 and LGG-2. lgg-1 is essential for degradation of protein aggregates, while lgg-2 has cargo-specific and developmental-stage-specific roles in aggregate degradation. Crystallography revealed that the N-terminal tails of LGG-1 and LGG-2 adopt the closed and open form, respectively. LGG-1 and LGG-2 interact differentially with autophagy substrates and Atg proteins, many of which carry a LIR motif. LGG-1 and LGG-2 have structurally distinct substrate binding pockets that prefer different residues in the interacting LIR motif, thus influencing binding specificity. Lipidated LGG-1 and LGG-2 possess distinct membrane tethering and fusion activities, which may result from the N-terminal differences. Our study reveals the differential function of two ATG8 homologs in autophagy during C. elegans development.
		+
		+	Structural Basis of the Differential Function of the Two C. elegans Atg8 Homologs, LGG-1 and LGG-2, in Autophagy.,Wu F, Watanabe Y, Guo XY, Qi X, Wang P, Zhao HY, Wang Z, Fujioka Y, Zhang H, Ren JQ, Fang TC, Shen YX, Feng W, Hu JJ, Noda NN, Zhang H Mol Cell. 2015 Dec 17;60(6):914-29. doi: 10.1016/j.molcel.2015.11.019. PMID:26687600<ref>PMID:26687600</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5azh" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Synthetic construct sequences]]
	[[Category: Fujioka, Y]]		[[Category: Fujioka, Y]]
	[[Category: Noda, N N]]		[[Category: Noda, N N]]

OCA at 19:36, 30 December 2015

OCA — Wed, 30 Dec 2015 19:36:55 GMT

←Older revision		Revision as of 19:36, 30 December 2015
Line 1:		Line 1:
-	'''~~Unreleased structure~~'''	+	==Crystal structure of LGG-2 fused with an EEEWEEL peptide==
-		+	<StructureSection load='5azh' size='340' side='right' caption='[[5azh]], [[Resolution\|resolution]] 2.30Å' scene=''>
-	~~The entry~~ 5azh is ~~ON HOLD until Paper Publication~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5azh]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5AZH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5AZH FirstGlance]. <br>
-	~~Authors~~: ~~Watanabe~~, Y., ~~Fujioka~~, Y., ~~Noda~~, N.N.	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5azf\|5azf]], [[5azg\|5azg]]</td></tr>
-	~~Description:~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5azh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5azh OCA], [http://pdbe.org/5azh PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5azh RCSB], [http://www.ebi.ac.uk/pdbsum/5azh PDBsum]</span></td></tr>
-	~~[[Category: Unreleased Structures]]~~	+	</table>
		+	__TOC__
		+	</StructureSection>
	[[Category: Fujioka, Y]]		[[Category: Fujioka, Y]]
		+	[[Category: Noda, N N]]
	[[Category: Watanabe, Y]]		[[Category: Watanabe, Y]]
-	[[Category: ~~Noda, N.N~~]]	+	[[Category: Autophagy]]
		+	[[Category: Protein binding]]
		+	[[Category: Ubiquitin-like]]

OCA: Protected "5azh" [edit=sysop:move=sysop]

OCA — Fri, 16 Oct 2015 00:58:18 GMT

Protected "5azh" [edit=sysop:move=sysop]

←Older revision

Revision as of 00:58, 16 October 2015

OCA: New page: '''Unreleased structure''' The entry 5azh is ON HOLD until Paper Publication Authors: Watanabe, Y., Fujioka, Y., Noda, N.N. Description: Category: Unreleased Structures [[Category...

OCA — Fri, 16 Oct 2015 00:58:16 GMT

New page: '''Unreleased structure''' The entry 5azh is ON HOLD until Paper Publication Authors: Watanabe, Y., Fujioka, Y., Noda, N.N. Description: Category: Unreleased Structures [[Category...

New page

'''Unreleased structure'''

The entry 5azh is ON HOLD until Paper Publication

Authors: Watanabe, Y., Fujioka, Y., Noda, N.N.

Description:
[[Category: Unreleased Structures]]
[[Category: Fujioka, Y]]
[[Category: Watanabe, Y]]
[[Category: Noda, N.N]]