5b7i - Revision history

OCA at 06:41, 31 May 2023

OCA — Wed, 31 May 2023 06:41:10 GMT

←Older revision		Revision as of 06:41, 31 May 2023
Line 3:		Line 3:
	<StructureSection load='5b7i' size='340' side='right'caption='[[5b7i]], [[Resolution\|resolution]] 2.60Å' scene=''>		<StructureSection load='5b7i' size='340' side='right'caption='[[5b7i]], [[Resolution\|resolution]] 2.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5b7i]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5B7I OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5B7I FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5b7i]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_UCBPP-PA14 Pseudomonas aeruginosa UCBPP-PA14] and [https://en.wikipedia.org/wiki/Pseudomonas_phage_JBD5 Pseudomonas phage JBD5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5B7I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5B7I FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-	~~<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat">~~<scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5b7i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5b7i OCA], [https://pdbe.org/5b7i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5b7i RCSB], [https://www.ebi.ac.uk/pdbsum/5b7i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5b7i ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5b7i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5b7i OCA], [~~http~~://pdbe.org/5b7i PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5b7i RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5b7i PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5b7i ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/CAS3_PSEAB CAS3_PSEAB]] CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Cas3 plus Cascade participate in CRISPR interference, the third stage of CRISPR immunity (Potential). In this bacteria Y.pestis-subtype CRISPRs do not confer resistance to phage DSM3 or MP22, but instead are required for DMS3-dependent inhibition of biofilm formation and possibly motility.<ref>PMID:21398535</ref>	+	[https://www.uniprot.org/uniprot/CAS3_PSEAB CAS3_PSEAB] CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Cas3 plus Cascade participate in CRISPR interference, the third stage of CRISPR immunity (Potential). In this bacteria Y.pestis-subtype CRISPRs do not confer resistance to phage DSM3 or MP22, but instead are required for DMS3-dependent inhibition of biofilm formation and possibly motility.<ref>PMID:21398535</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 27:		Line 26:
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Wang, X]]~~	+	[[Category: Pseudomonas aeruginosa UCBPP-PA14]]
-	~~[[Category: Zhu, Y]]~~	+	[[Category: Pseudomonas phage JBD5]]
-	~~[[Category: Anti~~-~~crispr~~]]	+	[[Category: Wang X]]
-	[[Category: ~~Dna nuclease~~]]	+	[[Category: Zhu Y]]
-	[[Category: ~~Hydrolase-unknown function complex~~]]	+
-	[[Category: ~~Phagy protein~~]]	+

OCA at 08:48, 11 March 2020

OCA — Wed, 11 Mar 2020 08:48:22 GMT

←Older revision		Revision as of 08:48, 11 March 2020
Line 1:		Line 1:

	==Cas3-AcrF3 complex==		==Cas3-AcrF3 complex==
-	<StructureSection load='5b7i' size='340' side='right' caption='[[5b7i]], [[Resolution\|resolution]] 2.60Å' scene=''>	+	<StructureSection load='5b7i' size='340' side='right'caption='[[5b7i]], [[Resolution\|resolution]] 2.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5b7i]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5B7I OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5B7I FirstGlance]. <br>		<table><tr><td colspan='2'>[[5b7i]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5B7I OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5B7I FirstGlance]. <br>
Line 21:		Line 21:

	==See Also==		==See Also==
-	*[[Helicase\|Helicase]]	+	*[[Helicase 3D structures\|Helicase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Wang, X]]		[[Category: Wang, X]]
	[[Category: Zhu, Y]]		[[Category: Zhu, Y]]

OCA at 09:27, 4 October 2017

OCA — Wed, 04 Oct 2017 09:27:37 GMT

←Older revision		Revision as of 09:27, 4 October 2017
Line 19:		Line 19:
	</div>		</div>
	<div class="pdbe-citations 5b7i" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5b7i" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Helicase\|Helicase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 08:12, 10 August 2016

OCA — Wed, 10 Aug 2016 08:12:33 GMT

←Older revision		Revision as of 08:12, 10 August 2016
Line 10:		Line 10:
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/CAS3_PSEAB CAS3_PSEAB]] CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Cas3 plus Cascade participate in CRISPR interference, the third stage of CRISPR immunity (Potential). In this bacteria Y.pestis-subtype CRISPRs do not confer resistance to phage DSM3 or MP22, but instead are required for DMS3-dependent inhibition of biofilm formation and possibly motility.<ref>PMID:21398535</ref>		[[http://www.uniprot.org/uniprot/CAS3_PSEAB CAS3_PSEAB]] CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Cas3 plus Cascade participate in CRISPR interference, the third stage of CRISPR immunity (Potential). In this bacteria Y.pestis-subtype CRISPRs do not confer resistance to phage DSM3 or MP22, but instead are required for DMS3-dependent inhibition of biofilm formation and possibly motility.<ref>PMID:21398535</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Bacteriophages express proteins that inactivate the CRISPR-Cas bacterial immune system. Here we report the crystal structure of the anti-CRISPR protein AcrF3 in complex with Pseudomonas aeruginosa Cas3 (PaCas3). AcrF3 forms a homodimer that locks PaCas3 in an ADP-bound form, blocks the entrance of the DNA-binding tunnel in the helicase domain, and masks the linker region and C-terminal domain of PaCas3, thereby preventing recruitment by Cascade and inhibiting the type I-F CRISPR-Cas system.
		+
		+	Structural basis of Cas3 inhibition by the bacteriophage protein AcrF3.,Wang X, Yao D, Xu JG, Li AR, Xu J, Fu P, Zhou Y, Zhu Y Nat Struct Mol Biol. 2016 Jul 25. doi: 10.1038/nsmb.3269. PMID:27455460<ref>PMID:27455460</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5b7i" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 17:56, 13 July 2016

OCA — Wed, 13 Jul 2016 17:56:28 GMT

←Older revision		Revision as of 17:56, 13 July 2016
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5b7i is ~~ON HOLD until Paper Publication~~	+	==Cas3-AcrF3 complex==
-		+	<StructureSection load='5b7i' size='340' side='right' caption='[[5b7i]], [[Resolution\|resolution]] 2.60Å' scene=''>
-	~~Authors~~: ~~Wang~~, X., ~~Zhu~~, Y.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5b7i]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5B7I OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5B7I FirstGlance]. <br>
-	~~Description~~: Cas3-~~AcrF3 complex~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5b7i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5b7i OCA], [http://pdbe.org/5b7i PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5b7i RCSB], [http://www.ebi.ac.uk/pdbsum/5b7i PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5b7i ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/CAS3_PSEAB CAS3_PSEAB]] CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Cas3 plus Cascade participate in CRISPR interference, the third stage of CRISPR immunity (Potential). In this bacteria Y.pestis-subtype CRISPRs do not confer resistance to phage DSM3 or MP22, but instead are required for DMS3-dependent inhibition of biofilm formation and possibly motility.<ref>PMID:21398535</ref>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Wang, X]]
	[[Category: Zhu, Y]]		[[Category: Zhu, Y]]
-	[[Category: ~~Wang, X~~]]	+	[[Category: Anti-crispr]]
		+	[[Category: Dna nuclease]]
		+	[[Category: Hydrolase-unknown function complex]]
		+	[[Category: Phagy protein]]

OCA at 16:47, 23 June 2016

OCA — Thu, 23 Jun 2016 16:47:44 GMT

←Older revision		Revision as of 16:47, 23 June 2016
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5b7i is ON HOLD	+	The entry 5b7i is ON HOLD until Paper Publication

	Authors: Wang, X., Zhu, Y.		Authors: Wang, X., Zhu, Y.

OCA: Protected "5b7i" [edit=sysop:move=sysop]

OCA — Mon, 20 Jun 2016 09:06:46 GMT

Protected "5b7i" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:06, 20 June 2016

OCA: New page: '''Unreleased structure''' The entry 5b7i is ON HOLD Authors: Wang, X., Zhu, Y. Description: Cas3-AcrF3 complex Category: Unreleased Structures Category: Zhu, Y [[Category: Wan...

OCA — Mon, 20 Jun 2016 09:06:45 GMT

New page: '''Unreleased structure''' The entry 5b7i is ON HOLD Authors: Wang, X., Zhu, Y. Description: Cas3-AcrF3 complex Category: Unreleased Structures Category: Zhu, Y [[Category: Wan...

New page

'''Unreleased structure'''

The entry 5b7i is ON HOLD

Authors: Wang, X., Zhu, Y.

Description: Cas3-AcrF3 complex
[[Category: Unreleased Structures]]
[[Category: Zhu, Y]]
[[Category: Wang, X]]