5bqk - Revision history

OCA at 06:04, 7 June 2023

OCA — Wed, 07 Jun 2023 06:04:09 GMT

←Older revision		Revision as of 06:04, 7 June 2023
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	<StructureSection load='5bqk' size='340' side='right'caption='[[5bqk]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='5bqk' size='340' side='right'caption='[[5bqk]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5bqk]] is a 3 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Hhv-1 Hhv-~~1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5BQK OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5BQK FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5bqk]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Human_alphaherpesvirus_1 Human alphaherpesvirus 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5BQK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5BQK FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"~~><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	~~<tr~~ id~~='NonStdRes'><td class~~="~~sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat~~"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene><~~/td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5bqk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5bqk OCA], [https://pdbe.org/5bqk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5bqk RCSB], [https://www.ebi.ac.uk/pdbsum/5bqk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5bqk ProSAT]</span></td></tr>
-	~~<tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b~~></td>~~<td class="sblockDat">UL54 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10298 HHV-1])~~</td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5bqk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5bqk OCA], [~~http~~://pdbe.org/5bqk PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5bqk RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5bqk PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5bqk ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/ICP27_HHV11 ICP27_HHV11] Multifunctional regulator of the expression of viral genes that contributes to the shutoff of host protein synthesis and mediates nuclear export of viral intronless mRNAs. Early in infection, this immediate early (EI) protein mediates the inhibition of cellular splicing. This results in the accumulation of unprocessed 3'end pre-mRNAs which can't be exported from the nucleus. Cellular protein synthesis is thereby shut off early after virus infection. Later in the infection, it helps recruit cellular RNA polymerase II to viral replication sites and promotes the nuclear export of viral intronless mRNAs by interacting with mRNAs and host NXF1/TAP. ICP27 binds to NUP62 which may provide facilitated viral mRNA export and may indirectly compete with some host cell transport receptors for binding and inhibit cellular nucleocytoplasmic transport pathways. Also stimulates translation of viral transcripts. Repression of host gene expression blocks the cell cycle at the G1 phase and prevents apoptosis. Seems to silence the 3' splice site of the promyelocytic leukemia (PML) intron 7a, thereby switching PML isoforms from PML-II to PML-V. This could be linked to the accelerated mRNA export induced by ICP27 which might not provide sufficient time for PML pre-mRNA to be spliced in the nucleus.<ref>PMID:11287586</ref> <ref>PMID:12660167</ref> <ref>PMID:16537625</ref> <ref>PMID:19369354</ref> <ref>PMID:19553338</ref> <ref>PMID:22334672</ref> <ref>PMID:9512520</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Hhv-~~1]]	+	[[Category: Human alphaherpesvirus 1]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Dahlroth, S]]	+	[[Category: Dahlroth S]]
-	[[Category: Nordlund, P]]	+	[[Category: Nordlund P]]
-	[[Category: Patel, V]]	+	[[Category: Patel V]]
-	[[Category: Rajakannan, V]]	+	[[Category: Rajakannan V]]
-	~~[[Category: Alpha-helical]]~~	+
-	~~[[Category: C-terminal domain]]~~	+
-	~~[[Category: Hsv-1]]~~	+
-	~~[[Category: Icp27]]~~	+
-	~~[[Category: Ul54]]~~	+
-	~~[[Category: Viral protein]]~~	+
-	~~[[Category: Zinc-binding motif~~]]	+

OCA at 08:54, 11 March 2020

OCA — Wed, 11 Mar 2020 08:54:34 GMT

←Older revision		Revision as of 08:54, 11 March 2020
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	==CRYSTAL STRUCTURE OF C-TERMINAL DOMAIN OF ICP27 PROTEIN FROM HSV-1==		==CRYSTAL STRUCTURE OF C-TERMINAL DOMAIN OF ICP27 PROTEIN FROM HSV-1==
-	<StructureSection load='5bqk' size='340' side='right' caption='[[5bqk]], [[Resolution\|resolution]] 2.00Å' scene=''>	+	<StructureSection load='5bqk' size='340' side='right'caption='[[5bqk]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5bqk]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5BQK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5BQK FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5bqk]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Hhv-1 Hhv-1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5BQK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5BQK FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">UL54 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10298 HHV-1])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5bqk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5bqk OCA], [http://pdbe.org/5bqk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5bqk RCSB], [http://www.ebi.ac.uk/pdbsum/5bqk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5bqk ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5bqk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5bqk OCA], [http://pdbe.org/5bqk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5bqk RCSB], [http://www.ebi.ac.uk/pdbsum/5bqk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5bqk ProSAT]</span></td></tr>
	</table>		</table>
Line 21:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Hhv-1]]
		+	[[Category: Large Structures]]
	[[Category: Dahlroth, S]]		[[Category: Dahlroth, S]]
	[[Category: Nordlund, P]]		[[Category: Nordlund, P]]

OCA at 16:06, 17 May 2017

OCA — Wed, 17 May 2017 16:06:20 GMT

←Older revision		Revision as of 16:06, 17 May 2017
Line 1:		Line 1:
		+
	==CRYSTAL STRUCTURE OF C-TERMINAL DOMAIN OF ICP27 PROTEIN FROM HSV-1==		==CRYSTAL STRUCTURE OF C-TERMINAL DOMAIN OF ICP27 PROTEIN FROM HSV-1==
	<StructureSection load='5bqk' size='340' side='right' caption='[[5bqk]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='5bqk' size='340' side='right' caption='[[5bqk]], [[Resolution\|resolution]] 2.00Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5bqk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5bqk OCA], [http://www.rcsb.org/pdb/explore.do?structureId=5bqk RCSB], [http://www.ebi.ac.uk/pdbsum/5bqk PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5bqk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5bqk OCA], [http://pdbe.org/5bqk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5bqk RCSB], [http://www.ebi.ac.uk/pdbsum/5bqk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5bqk ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 5bqk" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 12:05, 1 July 2015

OCA — Wed, 01 Jul 2015 12:05:06 GMT

←Older revision		Revision as of 12:05, 1 July 2015
Line 1:		Line 1:
-	'''~~Unreleased~~ structure'''	+	==CRYSTAL STRUCTURE OF C-TERMINAL DOMAIN OF ICP27 PROTEIN FROM HSV-1==
		+	<StructureSection load='5bqk' size='340' side='right' caption='[[5bqk]], [[Resolution\|resolution]] 2.00Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5bqk]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5BQK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5BQK FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5bqk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5bqk OCA], [http://www.rcsb.org/pdb/explore.do?structureId=5bqk RCSB], [http://www.ebi.ac.uk/pdbsum/5bqk PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Herpes viruses are nuclear-replicating viruses that have successfully evolved to evade the immune system of humans, establishing life-long infections. ICP27 from herpes simplex virus (HSV) is a multifunctional regulatory protein that is functionally conserved in all known human herpes viruses. It has the potential to interact with an array of cellular proteins as well as intronless viral RNAs. ICP27 plays an essential role in viral transcription, nuclear export of intronless RNAs, translation of viral transcripts and virion host shut-off function. It has also been implicated in several signaling pathways and prevention of apoptosis. Although much is known about its central role in viral replication and infection, very little is known about the structure and mechanistic properties of ICP27 and its homologs. We present the first crystal structure of ICP27 C-terminal domain at 2.0 A resolution. The structure reveals the C-terminal half of ICP27 to have a novel fold consisting of alpha-helices and long loops, along with a unique CHCC-type of zinc-binding motif. The two termini of this domain extend out from the central core and hint to possibilities of making interactions. ICP27 essential domain is capable of forming self-dimers as seen in the structure, which is confirmed by analytical ultracentrifugation study. Preliminary in vitro phosphorylation assays reveal that this domain may be regulated by cellular kinases. IMPORTANCE: ICP27 is a key regulatory protein of the Herpes Simplex Virus and has functional homologs in all known human herpes viruses. Understanding the structure of this protein is a step ahead in deciphering the mechanism by which the virus thrives. In this study, we present the first structure of the C-terminal domain of ICP27 and describe its novel features. We critically analyze the structure and compare our results to the information available form earlier studies. This structure can act as a guide in future experimental designs, and can add to a better understanding of mechanism of ICP27 as well as that of its homologs.

-	~~The entry 5bqk is ON HOLD~~	+	Structure of C-Terminal Domain of the Multifunctional ICP27 Protein from Herpes Simplex Virus-1.,Patel V, Dahlroth SL, Rajakannan V, Ho HT, Cornvik T, Nordlund P J Virol. 2015 Jun 17. pii: JVI.00441-15. PMID:26085142<ref>PMID:26085142</ref>

-	~~Authors: Patel~~, V.~~, Rajakannan, V., Dahlroth,~~ S.~~, Nordlund, P~~.	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~Description: CRYSTAL STRUCTURE OF C-TERMINAL DOMAIN OF ICP27 PROTEIN FROM HSV-1~~	+	== References ==
-	[[Category: ~~Unreleased Structures~~]]	+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Dahlroth, S]]
	[[Category: Nordlund, P]]		[[Category: Nordlund, P]]
	[[Category: Patel, V]]		[[Category: Patel, V]]
	[[Category: Rajakannan, V]]		[[Category: Rajakannan, V]]
-	[[Category: ~~Dahlroth, S~~]]	+	[[Category: Alpha-helical]]
		+	[[Category: C-terminal domain]]
		+	[[Category: Hsv-1]]
		+	[[Category: Icp27]]
		+	[[Category: Ul54]]
		+	[[Category: Viral protein]]
		+	[[Category: Zinc-binding motif]]

OCA: Protected "5bqk" [edit=sysop:move=sysop]

OCA — Wed, 03 Jun 2015 11:49:18 GMT

Protected "5bqk" [edit=sysop:move=sysop]

←Older revision

Revision as of 11:49, 3 June 2015

OCA: New page: '''Unreleased structure''' The entry 5bqk is ON HOLD Authors: Patel, V., Rajakannan, V., Dahlroth, S., Nordlund, P. Description: CRYSTAL STRUCTURE OF C-TERMINAL DOMAIN OF ICP27 PROTEIN...

OCA — Wed, 03 Jun 2015 11:49:16 GMT

New page: '''Unreleased structure''' The entry 5bqk is ON HOLD Authors: Patel, V., Rajakannan, V., Dahlroth, S., Nordlund, P. Description: CRYSTAL STRUCTURE OF C-TERMINAL DOMAIN OF ICP27 PROTEIN...

New page

'''Unreleased structure'''

The entry 5bqk is ON HOLD

Authors: Patel, V., Rajakannan, V., Dahlroth, S., Nordlund, P.

Description: CRYSTAL STRUCTURE OF C-TERMINAL DOMAIN OF ICP27 PROTEIN FROM HSV-1
[[Category: Unreleased Structures]]
[[Category: Nordlund, P]]
[[Category: Patel, V]]
[[Category: Rajakannan, V]]
[[Category: Dahlroth, S]]