5bv7 - Revision history

OCA at 08:31, 27 September 2023

OCA — Wed, 27 Sep 2023 08:31:03 GMT

←Older revision		Revision as of 08:31, 27 September 2023
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5bv7]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5BV7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5BV7 FirstGlance]. <br>		<table><tr><td colspan='2'>[[5bv7]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5BV7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5BV7 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.45Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5bv7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5bv7 OCA], [https://pdbe.org/5bv7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5bv7 RCSB], [https://www.ebi.ac.uk/pdbsum/5bv7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5bv7 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5bv7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5bv7 OCA], [https://pdbe.org/5bv7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5bv7 RCSB], [https://www.ebi.ac.uk/pdbsum/5bv7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5bv7 ProSAT]</span></td></tr>
	</table>		</table>

OCA at 06:09, 7 June 2023

OCA — Wed, 07 Jun 2023 06:09:24 GMT

←Older revision		Revision as of 06:09, 7 June 2023
Line 3:		Line 3:
	<StructureSection load='5bv7' size='340' side='right'caption='[[5bv7]], [[Resolution\|resolution]] 2.45Å' scene=''>		<StructureSection load='5bv7' size='340' side='right'caption='[[5bv7]], [[Resolution\|resolution]] 2.45Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5bv7]] is a 5 chain structure with sequence from [~~http://en.wikipedia.org/wiki/Human Human] and [http~~://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5BV7 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5BV7 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5bv7]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5BV7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5BV7 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphatidylcholine--sterol_O-acyltransferase Phosphatidylcholine--sterol O-acyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.43 2.3.1.43] </span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5bv7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5bv7 OCA], [https://pdbe.org/5bv7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5bv7 RCSB], [https://www.ebi.ac.uk/pdbsum/5bv7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5bv7 ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5bv7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5bv7 OCA], [~~http~~://pdbe.org/5bv7 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5bv7 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5bv7 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5bv7 ProSAT]</span></td></tr>	+
	</table>		</table>
	== Disease ==		== Disease ==
-	[~~[http~~://www.uniprot.org/uniprot/LCAT_HUMAN LCAT_HUMAN]] Fish-eye disease;Familial LCAT deficiency. The disease is caused by mutations affecting the gene represented in this entry. The disease is caused by mutations affecting the gene represented in this entry.	+	[https://www.uniprot.org/uniprot/LCAT_HUMAN LCAT_HUMAN] Fish-eye disease;Familial LCAT deficiency. The disease is caused by mutations affecting the gene represented in this entry. The disease is caused by mutations affecting the gene represented in this entry.
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/LCAT_HUMAN LCAT_HUMAN]] Central enzyme in the extracellular metabolism of plasma lipoproteins. Synthesized mainly in the liver and secreted into plasma where it converts cholesterol and phosphatidylcholines (lecithins) to cholesteryl esters and lysophosphatidylcholines on the surface of high and low density lipoproteins (HDLs and LDLs). The cholesterol ester is then transported back to the liver. Has a preference for plasma 16:0-18:2 or 18:O-18:2 phosphatidylcholines. Also produced in the brain by primary astrocytes, and esterifies free cholesterol on nascent APOE-containing lipoproteins secreted from glia and influences cerebral spinal fluid (CSF) APOE- and APOA1 levels. Together with APOE and the cholesterol transporter ABCA1, plays a key role in the maturation of glial-derived, nascent lipoproteins. Required for remodeling high-density lipoprotein particles into their spherical forms.<ref>PMID:10722751</ref>	+	[https://www.uniprot.org/uniprot/LCAT_HUMAN LCAT_HUMAN] Central enzyme in the extracellular metabolism of plasma lipoproteins. Synthesized mainly in the liver and secreted into plasma where it converts cholesterol and phosphatidylcholines (lecithins) to cholesteryl esters and lysophosphatidylcholines on the surface of high and low density lipoproteins (HDLs and LDLs). The cholesterol ester is then transported back to the liver. Has a preference for plasma 16:0-18:2 or 18:O-18:2 phosphatidylcholines. Also produced in the brain by primary astrocytes, and esterifies free cholesterol on nascent APOE-containing lipoproteins secreted from glia and influences cerebral spinal fluid (CSF) APOE- and APOA1 levels. Together with APOE and the cholesterol transporter ABCA1, plays a key role in the maturation of glial-derived, nascent lipoproteins. Required for remodeling high-density lipoprotein particles into their spherical forms.<ref>PMID:10722751</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</StructureSection>		</StructureSection>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
-	[[Category: Human]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	~~[[Category: Phosphatidylcholine--sterol O-acyltransferase]]~~	+	[[Category: Piper DE]]
-	[[Category: Piper~~, D E~~]]	+	[[Category: Romanow WG]]
-	[[Category: Romanow~~, W G~~]]	+	[[Category: Thibault ST]]
-	[[Category: Thibault~~, S T~~]]	+	[[Category: Walker NPC]]
-	[[Category: Walker~~, N P.C]]~~	+
-	~~[[Category: A/b hydrolase]]~~	+
-	~~[[Category: Hydrolase-immune system complex]]~~	+
-	~~[[Category: Immune system~~]]	+

OCA at 08:14, 27 May 2020

OCA — Wed, 27 May 2020 08:14:53 GMT

←Older revision		Revision as of 08:14, 27 May 2020
Line 1:		Line 1:

	==Crystal structure of human LCAT (L4F, N5D) in complex with Fab of an agonistic antibody==		==Crystal structure of human LCAT (L4F, N5D) in complex with Fab of an agonistic antibody==
-	<StructureSection load='5bv7' size='340' side='right' caption='[[5bv7]], [[Resolution\|resolution]] 2.45Å' scene=''>	+	<StructureSection load='5bv7' size='340' side='right'caption='[[5bv7]], [[Resolution\|resolution]] 2.45Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5bv7]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human] and [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5BV7 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5BV7 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5bv7]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human] and [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5BV7 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5BV7 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphatidylcholine--sterol_O-acyltransferase Phosphatidylcholine--sterol O-acyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.43 2.3.1.43] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphatidylcholine--sterol_O-acyltransferase Phosphatidylcholine--sterol O-acyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.43 2.3.1.43] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5bv7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5bv7 OCA], [http://pdbe.org/5bv7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5bv7 RCSB], [http://www.ebi.ac.uk/pdbsum/5bv7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5bv7 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5bv7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5bv7 OCA], [http://pdbe.org/5bv7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5bv7 RCSB], [http://www.ebi.ac.uk/pdbsum/5bv7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5bv7 ProSAT]</span></td></tr>
	</table>		</table>
	== Disease ==		== Disease ==
Line 27:		Line 27:
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Human]]		[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Phosphatidylcholine--sterol O-acyltransferase]]		[[Category: Phosphatidylcholine--sterol O-acyltransferase]]
	[[Category: Piper, D E]]		[[Category: Piper, D E]]

OCA at 06:23, 22 August 2018

OCA — Wed, 22 Aug 2018 06:23:53 GMT

←Older revision		Revision as of 06:23, 22 August 2018
Line 3:		Line 3:
	<StructureSection load='5bv7' size='340' side='right' caption='[[5bv7]], [[Resolution\|resolution]] 2.45Å' scene=''>		<StructureSection load='5bv7' size='340' side='right' caption='[[5bv7]], [[Resolution\|resolution]] 2.45Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5bv7]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/ ] and [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5BV7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5BV7 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5bv7]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human] and [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5BV7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5BV7 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphatidylcholine--sterol_O-acyltransferase Phosphatidylcholine--sterol O-acyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.43 2.3.1.43] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphatidylcholine--sterol_O-acyltransferase Phosphatidylcholine--sterol O-acyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.43 2.3.1.43] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5bv7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5bv7 OCA], [http://pdbe.org/5bv7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5bv7 RCSB], [http://www.ebi.ac.uk/pdbsum/5bv7 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5bv7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5bv7 OCA], [http://pdbe.org/5bv7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5bv7 RCSB], [http://www.ebi.ac.uk/pdbsum/5bv7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5bv7 ProSAT]</span></td></tr>
	</table>		</table>
	== Disease ==		== Disease ==
Line 26:		Line 26:
	</StructureSection>		</StructureSection>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
		+	[[Category: Human]]
	[[Category: Phosphatidylcholine--sterol O-acyltransferase]]		[[Category: Phosphatidylcholine--sterol O-acyltransferase]]
	[[Category: Piper, D E]]		[[Category: Piper, D E]]

OCA at 19:09, 20 February 2016

OCA — Sat, 20 Feb 2016 19:09:23 GMT

←Older revision		Revision as of 19:09, 20 February 2016
Line 1:		Line 1:
		+
	==Crystal structure of human LCAT (L4F, N5D) in complex with Fab of an agonistic antibody==		==Crystal structure of human LCAT (L4F, N5D) in complex with Fab of an agonistic antibody==
	<StructureSection load='5bv7' size='340' side='right' caption='[[5bv7]], [[Resolution\|resolution]] 2.45Å' scene=''>		<StructureSection load='5bv7' size='340' side='right' caption='[[5bv7]], [[Resolution\|resolution]] 2.45Å' scene=''>

OCA at 06:22, 23 December 2015

OCA — Wed, 23 Dec 2015 06:22:58 GMT

←Older revision		Revision as of 06:22, 23 December 2015
Line 11:		Line 11:
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/LCAT_HUMAN LCAT_HUMAN]] Central enzyme in the extracellular metabolism of plasma lipoproteins. Synthesized mainly in the liver and secreted into plasma where it converts cholesterol and phosphatidylcholines (lecithins) to cholesteryl esters and lysophosphatidylcholines on the surface of high and low density lipoproteins (HDLs and LDLs). The cholesterol ester is then transported back to the liver. Has a preference for plasma 16:0-18:2 or 18:O-18:2 phosphatidylcholines. Also produced in the brain by primary astrocytes, and esterifies free cholesterol on nascent APOE-containing lipoproteins secreted from glia and influences cerebral spinal fluid (CSF) APOE- and APOA1 levels. Together with APOE and the cholesterol transporter ABCA1, plays a key role in the maturation of glial-derived, nascent lipoproteins. Required for remodeling high-density lipoprotein particles into their spherical forms.<ref>PMID:10722751</ref>		[[http://www.uniprot.org/uniprot/LCAT_HUMAN LCAT_HUMAN]] Central enzyme in the extracellular metabolism of plasma lipoproteins. Synthesized mainly in the liver and secreted into plasma where it converts cholesterol and phosphatidylcholines (lecithins) to cholesteryl esters and lysophosphatidylcholines on the surface of high and low density lipoproteins (HDLs and LDLs). The cholesterol ester is then transported back to the liver. Has a preference for plasma 16:0-18:2 or 18:O-18:2 phosphatidylcholines. Also produced in the brain by primary astrocytes, and esterifies free cholesterol on nascent APOE-containing lipoproteins secreted from glia and influences cerebral spinal fluid (CSF) APOE- and APOA1 levels. Together with APOE and the cholesterol transporter ABCA1, plays a key role in the maturation of glial-derived, nascent lipoproteins. Required for remodeling high-density lipoprotein particles into their spherical forms.<ref>PMID:10722751</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Drug discovery opportunities where loss-of-function alleles of a target gene link to a disease-relevant phenotype often require an agonism approach to up-regulate or re-establish the activity of the target gene. Antibody therapy is increasingly recognized as a favored drug modality due to multiple desirable pharmacological properties. However, agonistic antibodies that enhance the activities of the target enzymes are rarely developed since the discovery of agonistic antibodies remains elusive. Here we report an innovative scheme of discovery and characterization of human antibodies capable of binding to and agonizing a circulating enzyme lecithin cholesterol acyltransferase (LCAT). Utilizing a modified human LCAT protein with enhanced enzymatic activity as an immunogen, we generated fully human monoclonal antibodies using the XenoMouseTM platform. One of the resultant agonistic antibodies, 27C3, binds to and substantially enhances the activity of LCAT from humans and cynomolgus macaques. X-ray crystallographic analysis of the 2.45 A LCAT/27C3 complex shows that 27C3 binding does not induce notable structural changes in LCAT. A single administration of 27C3 to cynomolgus monkeys led to a rapid increase of plasma LCAT enzymatic activity and a 35 % increase of the high density lipoprotein cholesterol that was observed up to 32 days post 27C3 administration. Thus, this novel scheme of immunization in conjunction with high throughput screening may represent an effective strategy for discovering agonistic antibodies against other enzyme targets. 27C3 and other agonistic human anti-human LCAT monoclonal antibodies described herein hold potential for therapeutic development for the treatment of dyslipidemia and cardiovascular disease.
		+
		+	Agonistic Human Antibodies Binding To Lecithin-Cholesterol Acyltransferase Modulate High Density Lipoprotein Metabolism.,Gunawardane RN, Fordstrom P, Piper DE, Masterman S, Siu S, Liu D, Brown M, Lu M, Tang J, Zhang R, Cheng J, Gates A, Meininger D, Chan J, Carlson T, Walker N, Schwarz M, Delaney J, Zhou M J Biol Chem. 2015 Dec 7. pii: jbc.M115.672790. PMID:26644477<ref>PMID:26644477</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5bv7" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 13:56, 16 December 2015

OCA — Wed, 16 Dec 2015 13:56:15 GMT

←Older revision		Revision as of 13:56, 16 December 2015
Line 1:		Line 1:
-	'''~~Unreleased structure~~'''	+	==Crystal structure of human LCAT (L4F, N5D) in complex with Fab of an agonistic antibody==
-		+	<StructureSection load='5bv7' size='340' side='right' caption='[[5bv7]], [[Resolution\|resolution]] 2.45Å' scene=''>
-	~~The entry~~ 5bv7 is ~~ON HOLD until Paper Publication~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5bv7]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/ ] and [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5BV7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5BV7 FirstGlance]. <br>
-	~~Authors~~: ~~Piper~~, D.E., ~~Romanow~~, W.G., ~~Thibault~~, S.T., ~~Walker~~, N.P.C.	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
-		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphatidylcholine--sterol_O-acyltransferase Phosphatidylcholine--sterol O-acyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.43 2.3.1.43] </span></td></tr>
-	~~Description~~:	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5bv7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5bv7 OCA], [http://pdbe.org/5bv7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5bv7 RCSB], [http://www.ebi.ac.uk/pdbsum/5bv7 PDBsum]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
-	[[Category: Piper, D.E]]	+	== Disease ==
-	[[Category: Romanow, W.G]]	+	[[http://www.uniprot.org/uniprot/LCAT_HUMAN LCAT_HUMAN]] Fish-eye disease;Familial LCAT deficiency. The disease is caused by mutations affecting the gene represented in this entry. The disease is caused by mutations affecting the gene represented in this entry.
-	[[Category: Walker, N.P.C]]	+	== Function ==
-	[[Category: ~~Thibault, S.T~~]]	+	[[http://www.uniprot.org/uniprot/LCAT_HUMAN LCAT_HUMAN]] Central enzyme in the extracellular metabolism of plasma lipoproteins. Synthesized mainly in the liver and secreted into plasma where it converts cholesterol and phosphatidylcholines (lecithins) to cholesteryl esters and lysophosphatidylcholines on the surface of high and low density lipoproteins (HDLs and LDLs). The cholesterol ester is then transported back to the liver. Has a preference for plasma 16:0-18:2 or 18:O-18:2 phosphatidylcholines. Also produced in the brain by primary astrocytes, and esterifies free cholesterol on nascent APOE-containing lipoproteins secreted from glia and influences cerebral spinal fluid (CSF) APOE- and APOA1 levels. Together with APOE and the cholesterol transporter ABCA1, plays a key role in the maturation of glial-derived, nascent lipoproteins. Required for remodeling high-density lipoprotein particles into their spherical forms.<ref>PMID:10722751</ref>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Homo sapiens]]
		+	[[Category: Phosphatidylcholine--sterol O-acyltransferase]]
		+	[[Category: Piper, D E]]
		+	[[Category: Romanow, W G]]
		+	[[Category: Thibault, S T]]
		+	[[Category: Walker, N P.C]]
		+	[[Category: A/b hydrolase]]
		+	[[Category: Hydrolase-immune system complex]]
		+	[[Category: Immune system]]

OCA at 10:51, 1 July 2015

OCA — Wed, 01 Jul 2015 10:51:34 GMT

←Older revision		Revision as of 10:51, 1 July 2015
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5bv7 is ON HOLD	+	The entry 5bv7 is ON HOLD until Paper Publication

	Authors: Piper, D.E., Romanow, W.G., Thibault, S.T., Walker, N.P.C.		Authors: Piper, D.E., Romanow, W.G., Thibault, S.T., Walker, N.P.C.

OCA: Protected "5bv7" [edit=sysop:move=sysop]

OCA — Wed, 10 Jun 2015 13:07:56 GMT

Protected "5bv7" [edit=sysop:move=sysop]

←Older revision

Revision as of 13:07, 10 June 2015

OCA: New page: '''Unreleased structure''' The entry 5bv7 is ON HOLD Authors: Piper, D.E., Romanow, W.G., Thibault, S.T., Walker, N.P.C. Description: Category: Unreleased Structures [[Category: P...

OCA — Wed, 10 Jun 2015 13:07:55 GMT

New page: '''Unreleased structure''' The entry 5bv7 is ON HOLD Authors: Piper, D.E., Romanow, W.G., Thibault, S.T., Walker, N.P.C. Description: Category: Unreleased Structures [[Category: P...

New page

'''Unreleased structure'''

The entry 5bv7 is ON HOLD

Authors: Piper, D.E., Romanow, W.G., Thibault, S.T., Walker, N.P.C.

Description:
[[Category: Unreleased Structures]]
[[Category: Piper, D.E]]
[[Category: Romanow, W.G]]
[[Category: Walker, N.P.C]]
[[Category: Thibault, S.T]]