5bw8 - Revision history

OCA at 08:31, 27 September 2023

OCA — Wed, 27 Sep 2023 08:31:47 GMT

←Older revision		Revision as of 08:31, 27 September 2023
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5bw8]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae], [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_RM11-1a Saccharomyces cerevisiae RM11-1a] and [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5BW8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5BW8 FirstGlance]. <br>		<table><tr><td colspan='2'>[[5bw8]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae], [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_RM11-1a Saccharomyces cerevisiae RM11-1a] and [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5BW8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5BW8 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.8Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5bw8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5bw8 OCA], [https://pdbe.org/5bw8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5bw8 RCSB], [https://www.ebi.ac.uk/pdbsum/5bw8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5bw8 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5bw8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5bw8 OCA], [https://pdbe.org/5bw8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5bw8 RCSB], [https://www.ebi.ac.uk/pdbsum/5bw8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5bw8 ProSAT]</span></td></tr>
	</table>		</table>

OCA at 06:10, 7 June 2023

OCA — Wed, 07 Jun 2023 06:10:50 GMT

←Older revision		Revision as of 06:10, 7 June 2023
Line 1:		Line 1:

	==2.8 A crystal structure of a Get3-Get4-Get5 intermediate complex from S.cerevisiae==		==2.8 A crystal structure of a Get3-Get4-Get5 intermediate complex from S.cerevisiae==
-	<StructureSection load='5bw8' size='340' side='right' caption='[[5bw8]], [[Resolution\|resolution]] 2.80Å' scene=''>	+	<StructureSection load='5bw8' size='340' side='right'caption='[[5bw8]], [[Resolution\|resolution]] 2.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5bw8]] is a 5 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Atcc_18824 Atcc 18824~~] and [~~http~~://en.wikipedia.org/wiki/~~Baker's_yeast Baker's yeast~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5BW8 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5BW8 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5bw8]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae], [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_RM11-1a Saccharomyces cerevisiae RM11-1a] and [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5BW8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5BW8 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	~~<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=UNK:UNKNOWN'>UNK</scene></td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5bw8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5bw8 OCA], [https://pdbe.org/5bw8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5bw8 RCSB], [https://www.ebi.ac.uk/pdbsum/5bw8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5bw8 ProSAT]</span></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5bwk\|5bwk]]</td></tr>~~	+
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GET3, SCRG_00595 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=285006 Baker's yeast]), GET4, YOR164C, O3580 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast]), MDY2, GET5, TMA24, YOL111C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast])</td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5bw8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5bw8 OCA], [~~http~~://pdbe.org/5bw8 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5bw8 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5bw8 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5bw8 ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/~~GET3_YEAS1 GET3_YEAS1]~~] ATPase required for the post-translational delivery of tail-anchored (TA) proteins to the endoplasmic reticulum. Recognizes and selectively binds the transmembrane domain of TA proteins in the cytosol. This complex then targets to the endoplasmic reticulum by membrane-bound receptors GET1 and GET2, where the tail-anchored protein is released for insertion. This process is regulated by ATP binding and hydrolysis. ATP binding drives the homodimer towards the closed dimer state, facilitating recognition of newly synthesized TA membrane proteins. ATP hydrolysis is required for insertion. Subsequently, the homodimer reverts towards the open dimer state, lowering its affinity for the GET1-GET2 receptor, and returning it to the cytosol to initiate a new round of targeting. Cooperates with the HDEL receptor ERD2 to mediate the ATP-dependent retrieval of resident ER proteins that contain a C-terminal H-D-E-L retention signal from the Golgi to the ER. Involved in low-level resistance to the oxyanions arsenite and arsenate, and in heat tolerance. ~~[[http~~://www.uniprot.org/uniprot/MDY2_YEAST MDY2_YEAST]] Required for efficient mating. Involved in the production of alpha-factor, the KAR9 and TUB1 location to the shmoo tip and nuclear migration into pheromone-induced shmoos.<ref>PMID:~~10514570~~</ref> <ref>PMID:~~16390866~~</ref> ~~[[http~~:/~~/www.uniprot.org/uniprot/GET4_YEAST GET4_YEAST]] May play a role in insertion of tail-anchored proteins into the endoplasmic reticulum membrane.~~<ref>PMID:~~19325107~~</ref>	+	[https://www.uniprot.org/uniprot/GET3_YEAST GET3_YEAST] ATPase required for the post-translational delivery of tail-anchored (TA) proteins to the endoplasmic reticulum. Recognizes and selectively binds the transmembrane domain of TA proteins in the cytosol. This complex then targets to the endoplasmic reticulum by membrane-bound receptors GET1 and GET2, where the tail-anchored protein is released for insertion. This process is regulated by ATP binding and hydrolysis. ATP binding drives the homodimer towards the closed dimer state, facilitating recognition of newly synthesized TA membrane proteins. ATP hydrolysis is required for insertion. Subsequently, the homodimer reverts towards the open dimer state, lowering its affinity for the GET1-GET2 receptor, and returning it to the cytosol to initiate a new round of targeting. Cooperates with the HDEL receptor ERD2 to mediate the ATP-dependent retrieval of resident ER proteins that contain a C-terminal H-D-E-L retention signal from the Golgi to the ER. Involved in low-level resistance to the oxyanions arsenite and arsenate, and in heat tolerance.<ref>PMID:12680698</ref> <ref>PMID:16269340</ref> <ref>PMID:18724936</ref> <ref>PMID:21866104</ref> <ref>PMID:21719644</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 21:		Line 18:
	</div>		</div>
	<div class="pdbe-citations 5bw8" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5bw8" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[ATPase 3D structures\|ATPase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Atcc 18824~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Baker's yeast~~]]	+	[[Category: Saccharomyces cerevisiae]]
-	[[Category: ~~Chartron, J W~~]]	+	[[Category: Saccharomyces cerevisiae RM11-1a]]
-	[[Category: ~~Clemons, W M~~]]	+	[[Category: Saccharomyces cerevisiae S288C]]
-	[[Category: ~~Gristick, H B~~]]	+	[[Category: Chartron JW]]
-	[[Category: ~~Atpase~~]]	+	[[Category: Clemons WM]]
-	[[Category: ~~Electrostatic interaction]]~~	+	[[Category: Gristick HB]]
-	~~[[Category: Hydrolase-transport complex]]~~	+
-	~~[[Category: Tail-anchored targeting~~]]	+

OCA at 09:48, 22 November 2017

OCA — Wed, 22 Nov 2017 09:48:07 GMT

←Older revision		Revision as of 09:48, 22 November 2017
Line 1:		Line 1:
		+
	==2.8 A crystal structure of a Get3-Get4-Get5 intermediate complex from S.cerevisiae==		==2.8 A crystal structure of a Get3-Get4-Get5 intermediate complex from S.cerevisiae==
	<StructureSection load='5bw8' size='340' side='right' caption='[[5bw8]], [[Resolution\|resolution]] 2.80Å' scene=''>		<StructureSection load='5bw8' size='340' side='right' caption='[[5bw8]], [[Resolution\|resolution]] 2.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5bw8]] is a 5 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5BW8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5BW8 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5bw8]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824] and [http://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5BW8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5BW8 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=UNK:UNKNOWN'>UNK</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=UNK:UNKNOWN'>UNK</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5bwk\|5bwk]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5bwk\|5bwk]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5bw8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5bw8 OCA], [http://pdbe.org/5bw8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5bw8 RCSB], [http://www.ebi.ac.uk/pdbsum/5bw8 PDBsum]</span></td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GET3, SCRG_00595 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=285006 Baker's yeast]), GET4, YOR164C, O3580 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast]), MDY2, GET5, TMA24, YOL111C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5bw8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5bw8 OCA], [http://pdbe.org/5bw8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5bw8 RCSB], [http://www.ebi.ac.uk/pdbsum/5bw8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5bw8 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 23:		Line 25:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Atcc 18824]]
		+	[[Category: Baker's yeast]]
	[[Category: Chartron, J W]]		[[Category: Chartron, J W]]
	[[Category: Clemons, W M]]		[[Category: Clemons, W M]]

OCA at 23:45, 21 October 2015

OCA — Wed, 21 Oct 2015 23:45:04 GMT

←Older revision		Revision as of 23:45, 21 October 2015
Line 10:		Line 10:
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/GET3_YEAS1 GET3_YEAS1]] ATPase required for the post-translational delivery of tail-anchored (TA) proteins to the endoplasmic reticulum. Recognizes and selectively binds the transmembrane domain of TA proteins in the cytosol. This complex then targets to the endoplasmic reticulum by membrane-bound receptors GET1 and GET2, where the tail-anchored protein is released for insertion. This process is regulated by ATP binding and hydrolysis. ATP binding drives the homodimer towards the closed dimer state, facilitating recognition of newly synthesized TA membrane proteins. ATP hydrolysis is required for insertion. Subsequently, the homodimer reverts towards the open dimer state, lowering its affinity for the GET1-GET2 receptor, and returning it to the cytosol to initiate a new round of targeting. Cooperates with the HDEL receptor ERD2 to mediate the ATP-dependent retrieval of resident ER proteins that contain a C-terminal H-D-E-L retention signal from the Golgi to the ER. Involved in low-level resistance to the oxyanions arsenite and arsenate, and in heat tolerance. [[http://www.uniprot.org/uniprot/MDY2_YEAST MDY2_YEAST]] Required for efficient mating. Involved in the production of alpha-factor, the KAR9 and TUB1 location to the shmoo tip and nuclear migration into pheromone-induced shmoos.<ref>PMID:10514570</ref> <ref>PMID:16390866</ref> [[http://www.uniprot.org/uniprot/GET4_YEAST GET4_YEAST]] May play a role in insertion of tail-anchored proteins into the endoplasmic reticulum membrane.<ref>PMID:19325107</ref>		[[http://www.uniprot.org/uniprot/GET3_YEAS1 GET3_YEAS1]] ATPase required for the post-translational delivery of tail-anchored (TA) proteins to the endoplasmic reticulum. Recognizes and selectively binds the transmembrane domain of TA proteins in the cytosol. This complex then targets to the endoplasmic reticulum by membrane-bound receptors GET1 and GET2, where the tail-anchored protein is released for insertion. This process is regulated by ATP binding and hydrolysis. ATP binding drives the homodimer towards the closed dimer state, facilitating recognition of newly synthesized TA membrane proteins. ATP hydrolysis is required for insertion. Subsequently, the homodimer reverts towards the open dimer state, lowering its affinity for the GET1-GET2 receptor, and returning it to the cytosol to initiate a new round of targeting. Cooperates with the HDEL receptor ERD2 to mediate the ATP-dependent retrieval of resident ER proteins that contain a C-terminal H-D-E-L retention signal from the Golgi to the ER. Involved in low-level resistance to the oxyanions arsenite and arsenate, and in heat tolerance. [[http://www.uniprot.org/uniprot/MDY2_YEAST MDY2_YEAST]] Required for efficient mating. Involved in the production of alpha-factor, the KAR9 and TUB1 location to the shmoo tip and nuclear migration into pheromone-induced shmoos.<ref>PMID:10514570</ref> <ref>PMID:16390866</ref> [[http://www.uniprot.org/uniprot/GET4_YEAST GET4_YEAST]] May play a role in insertion of tail-anchored proteins into the endoplasmic reticulum membrane.<ref>PMID:19325107</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Tail-anchored (TA) proteins, defined as having a single transmembrane helix at their C-terminus, are post-translationally targeted to the endoplasmic reticulum (ER) membrane by the GET (Guided Entry of TA proteins) pathway. In yeast, the handover of TA substrates is mediated by the heterotetrameric Get4/Get5 (Get4/5) complex, which tethers the co-chaperone Sgt2 to the targeting factor, the Get3 ATPase. Binding of Get4/5 to Get3 is critical for efficient TA targeting; however, questions remain about the formation of the Get3-Get4/5 complex. Here we report crystal structures of a Get3-Get4/5 complex from Saccharomyces cerevisiae (Sc) at 2.8 A and 6.0 A, which reveal a novel interface between Get3 and Get4 dominated by electrostatic interactions. Kinetic and mutational analyses strongly suggest that these structures represent an on-pathway intermediate that rapidly assembles and then rearranges to the final Get3-Get4/5 complex. Furthermore, we provide evidence that the Get3-Get4/5 complex is dominated by a single Get4/5 heterotetramer bound to one monomer of a Get3 dimer, uncovering an intriguing asymmetry in the Get4/5 heterotetramer upon Get3 binding. Ultrafast diffusion-limitd electrostatically driven Get3-Get4/5 association enables Get4/5 to rapidly sample and capture Get3 at different stages of the GET pathway.
		+
		+	Mechanism of assembly of a substrate-transfer complex during tail-anchored protein targeting.,Gristick HB, Rome ME, Chartron JW, Rao M, Hess S, Shan SO, Clemons WM Jr J Biol Chem. 2015 Oct 7. pii: jbc.M115.677328. PMID:26451041<ref>PMID:26451041</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5bw8" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 04:07, 16 October 2015

OCA — Fri, 16 Oct 2015 04:07:15 GMT

←Older revision		Revision as of 04:07, 16 October 2015
Line 1:		Line 1:
-	'''~~Unreleased structure~~'''	+	==2.8 A crystal structure of a Get3-Get4-Get5 intermediate complex from S.cerevisiae==
-		+	<StructureSection load='5bw8' size='340' side='right' caption='[[5bw8]], [[Resolution\|resolution]] 2.80Å' scene=''>
-	~~The entry~~ 5bw8 is ~~ON HOLD until Paper Publication~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5bw8]] is a 5 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5BW8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5BW8 FirstGlance]. <br>
-	~~Authors~~: ~~Gristick~~, H.B., ~~Chartron~~, J.W., ~~Clemons, W~~.M.	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-		+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=UNK:UNKNOWN'>UNK</scene></td></tr>
-	~~Description~~: 2.~~8 A crystal structure~~ of a ~~Get3~~-~~Get4~~-~~Get5 intermediate complex~~ from S.~~cerevisiae~~	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5bwk\|5bwk]]</td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5bw8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5bw8 OCA], [http://pdbe.org/5bw8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5bw8 RCSB], [http://www.ebi.ac.uk/pdbsum/5bw8 PDBsum]</span></td></tr>
-	[[~~Category~~: ~~Gristick, H~~.B]]	+	</table>
-	[[Category: Chartron, J.W]]	+	== Function ==
-	[[Category: Clemons, W.M]]	+	[[http://www.uniprot.org/uniprot/GET3_YEAS1 GET3_YEAS1]] ATPase required for the post-translational delivery of tail-anchored (TA) proteins to the endoplasmic reticulum. Recognizes and selectively binds the transmembrane domain of TA proteins in the cytosol. This complex then targets to the endoplasmic reticulum by membrane-bound receptors GET1 and GET2, where the tail-anchored protein is released for insertion. This process is regulated by ATP binding and hydrolysis. ATP binding drives the homodimer towards the closed dimer state, facilitating recognition of newly synthesized TA membrane proteins. ATP hydrolysis is required for insertion. Subsequently, the homodimer reverts towards the open dimer state, lowering its affinity for the GET1-GET2 receptor, and returning it to the cytosol to initiate a new round of targeting. Cooperates with the HDEL receptor ERD2 to mediate the ATP-dependent retrieval of resident ER proteins that contain a C-terminal H-D-E-L retention signal from the Golgi to the ER. Involved in low-level resistance to the oxyanions arsenite and arsenate, and in heat tolerance. [[http://www.uniprot.org/uniprot/MDY2_YEAST MDY2_YEAST]] Required for efficient mating. Involved in the production of alpha-factor, the KAR9 and TUB1 location to the shmoo tip and nuclear migration into pheromone-induced shmoos.<ref>PMID:10514570</ref> <ref>PMID:16390866</ref> [[http://www.uniprot.org/uniprot/GET4_YEAST GET4_YEAST]] May play a role in insertion of tail-anchored proteins into the endoplasmic reticulum membrane.<ref>PMID:19325107</ref>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Chartron, J W]]
		+	[[Category: Clemons, W M]]
		+	[[Category: Gristick, H B]]
		+	[[Category: Atpase]]
		+	[[Category: Electrostatic interaction]]
		+	[[Category: Hydrolase-transport complex]]
		+	[[Category: Tail-anchored targeting]]

OCA at 12:34, 22 July 2015

OCA — Wed, 22 Jul 2015 12:34:23 GMT

←Older revision		Revision as of 12:34, 22 July 2015
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5bw8 is ON HOLD until ~~sometime in the future~~	+	The entry 5bw8 is ON HOLD until Paper Publication

	Authors: Gristick, H.B., Chartron, J.W., Clemons, W.M.		Authors: Gristick, H.B., Chartron, J.W., Clemons, W.M.

OCA at 11:57, 15 July 2015

OCA — Wed, 15 Jul 2015 11:57:48 GMT

←Older revision		Revision as of 11:57, 15 July 2015
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5bw8 is ON HOLD until ~~Paper Publication~~	+	The entry 5bw8 is ON HOLD until sometime in the future

	Authors: Gristick, H.B., Chartron, J.W., Clemons, W.M.		Authors: Gristick, H.B., Chartron, J.W., Clemons, W.M.

-	Description: ~~Crystal~~ structure of a Get3-Get4-Get5 intermediate complex from S.cerevisiae	+	Description: 2.8 A crystal structure of a Get3-Get4-Get5 intermediate complex from S.cerevisiae
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: Gristick, H.B]]		[[Category: Gristick, H.B]]
	[[Category: Chartron, J.W]]		[[Category: Chartron, J.W]]
	[[Category: Clemons, W.M]]		[[Category: Clemons, W.M]]

OCA at 11:44, 24 June 2015

OCA — Wed, 24 Jun 2015 11:44:35 GMT

←Older revision		Revision as of 11:44, 24 June 2015
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5bw8 is ON HOLD	+	The entry 5bw8 is ON HOLD until Paper Publication

	Authors: Gristick, H.B., Chartron, J.W., Clemons, W.M.		Authors: Gristick, H.B., Chartron, J.W., Clemons, W.M.

OCA: Protected "5bw8" [edit=sysop:move=sysop]

OCA — Wed, 17 Jun 2015 13:58:16 GMT

Protected "5bw8" [edit=sysop:move=sysop]

←Older revision

Revision as of 13:58, 17 June 2015

OCA: New page: '''Unreleased structure''' The entry 5bw8 is ON HOLD Authors: Gristick, H.B., Chartron, J.W., Clemons, W.M. Description: Crystal structure of a Get3-Get4-Get5 intermediate complex from...

OCA — Wed, 17 Jun 2015 13:58:15 GMT

New page: '''Unreleased structure''' The entry 5bw8 is ON HOLD Authors: Gristick, H.B., Chartron, J.W., Clemons, W.M. Description: Crystal structure of a Get3-Get4-Get5 intermediate complex from...

New page

'''Unreleased structure'''

The entry 5bw8 is ON HOLD

Authors: Gristick, H.B., Chartron, J.W., Clemons, W.M.

Description: Crystal structure of a Get3-Get4-Get5 intermediate complex from S.cerevisiae
[[Category: Unreleased Structures]]
[[Category: Gristick, H.B]]
[[Category: Chartron, J.W]]
[[Category: Clemons, W.M]]