5bza - Revision history

OCA at 06:13, 7 June 2023

OCA — Wed, 07 Jun 2023 06:13:28 GMT

←Older revision		Revision as of 06:13, 7 June 2023
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	<StructureSection load='5bza' size='340' side='right'caption='[[5bza]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='5bza' size='340' side='right'caption='[[5bza]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5bza]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Atcc_49049 Atcc 49049~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5BZA OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5BZA FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5bza]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermotoga_neapolitana Thermotoga neapolitana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5BZA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5BZA FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CD:CADMIUM+ION'>CD</scene~~></td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CD:CADMIUM+ION'>CD</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5c0q\|5c0q]]</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5bza FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5bza OCA], [https://pdbe.org/5bza PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5bza RCSB], [https://www.ebi.ac.uk/pdbsum/5bza PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5bza ProSAT]</span></td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">cbsA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2337 ATCC 49049])</td></tr>~~	+
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-N-acetylhexosaminidase Beta-N-acetylhexosaminidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.52 3.2.1.52] </span></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5bza FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5bza OCA], [~~http~~://pdbe.org/5bza PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5bza RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5bza PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5bza ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q9AG27_THENE Q9AG27_THENE]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</div>		</div>
	<div class="pdbe-citations 5bza" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5bza" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Beta-N-acetylhexosaminidase 3D structures\|Beta-N-acetylhexosaminidase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Atcc 49049]]
-	[[Category: Beta-N-acetylhexosaminidase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Ha, N C~~]]	+	[[Category: Thermotoga neapolitana]]
-	[[Category: ~~Kim, J S~~]]	+	[[Category: Ha NC]]
-	[[Category: ~~Yoon, B Y~~]]	+	[[Category: Kim JS]]
-	[[Category: ~~Beta-n-acetylglucosaminidase]]~~	+	[[Category: Yoon BY]]
-	~~[[Category: Cbsa]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Thermostable enzyme]]~~	+
-	~~[[Category: Thermotoga~~]]	+

OCA at 07:09, 19 February 2020

OCA — Wed, 19 Feb 2020 07:09:08 GMT

←Older revision		Revision as of 07:09, 19 February 2020
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	==Crystal structure of CbsA from Thermotoga neapolitana==		==Crystal structure of CbsA from Thermotoga neapolitana==
-	<StructureSection load='5bza' size='340' side='right' caption='[[5bza]], [[Resolution\|resolution]] 2.00Å' scene=''>	+	<StructureSection load='5bza' size='340' side='right'caption='[[5bza]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5bza]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_49049 Atcc 49049]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5BZA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5BZA FirstGlance]. <br>		<table><tr><td colspan='2'>[[5bza]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_49049 Atcc 49049]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5BZA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5BZA FirstGlance]. <br>
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	[[Category: Atcc 49049]]		[[Category: Atcc 49049]]
	[[Category: Beta-N-acetylhexosaminidase]]		[[Category: Beta-N-acetylhexosaminidase]]
		+	[[Category: Large Structures]]
	[[Category: Ha, N C]]		[[Category: Ha, N C]]
	[[Category: Kim, J S]]		[[Category: Kim, J S]]

OCA at 17:12, 16 November 2017

OCA — Thu, 16 Nov 2017 17:12:47 GMT

←Older revision		Revision as of 17:12, 16 November 2017
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-	'''Unreleased structure'''

-	~~The entry~~ 5bza is ~~ON HOLD~~	+	==Crystal structure of CbsA from Thermotoga neapolitana==
		+	<StructureSection load='5bza' size='340' side='right' caption='[[5bza]], [[Resolution\|resolution]] 2.00Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5bza]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_49049 Atcc 49049]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5BZA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5BZA FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CD:CADMIUM+ION'>CD</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5c0q\|5c0q]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">cbsA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2337 ATCC 49049])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-N-acetylhexosaminidase Beta-N-acetylhexosaminidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.52 3.2.1.52] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5bza FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5bza OCA], [http://pdbe.org/5bza PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5bza RCSB], [http://www.ebi.ac.uk/pdbsum/5bza PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5bza ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	CbsA from the thermophilic marine bacteria Thermotoga neapolitana is a chitinolyitc enzyme that can cleave a glycosidic bond of the polymer N-acetylglucosamine at the non-reducing end. This enzyme has particularly high activity on di-N-acetylchitobiose. CbsA consists of a family of 3 glycoside hydrolase (GH3)-type catalytic domains and a unique C-terminal domain. The C-terminal domain distinguishes CbsA from other GH3-type enzymes. Sequence analyses have suggested that CbsA has the Asp-His dyad as a general acid/base with the NagZ of Bacillus subtilis and the Salmonella enterica serovar Typhimurium. Here, we determined the crystal structure of CbsA from T. neapolitana at a resolution of 2.0 A using the Zn-SAD method, revealing a unique homodimeric assembly facilitated by the C-terminal domains in the dimer. We observed that CbsA is strongly inhibited by ZnCl2, and two zinc ions were consistently bound in the active site. Our results can explain the zinc ion's inhibition mechanism in the subfamily of GH3 enzymes, and provide information on the structural diversity and substrate specificity of this hydrolase family.

-	~~Authors: Ha,~~ N.C., Kim, J.S., ~~Yoon, B~~.Y.	+	Crystal structure of beta-N-acetylglucosaminidase CbsA from Thermotoga neapolitana.,Kim JS, Yoon BY, Ahn J, Cha J, Ha NC Biochem Biophys Res Commun. 2015 Aug 28;464(3):869-74. doi:, 10.1016/j.bbrc.2015.07.053. Epub 2015 Jul 14. PMID:26187666<ref>PMID:26187666</ref>

-	~~Description: Crystal structure~~ of ~~CbsA from Thermotoga neapolitana~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
-	[[Category: Kim, J.S]]	+	<div class="pdbe-citations 5bza" style="background-color:#fffaf0;"></div>
-	[[Category: Yoon, B.Y]]	+	== References ==
-	[[Category: ~~Ha, N.C~~]]	+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Atcc 49049]]
		+	[[Category: Beta-N-acetylhexosaminidase]]
		+	[[Category: Ha, N C]]
		+	[[Category: Kim, J S]]
		+	[[Category: Yoon, B Y]]
		+	[[Category: Beta-n-acetylglucosaminidase]]
		+	[[Category: Cbsa]]
		+	[[Category: Hydrolase]]
		+	[[Category: Thermostable enzyme]]
		+	[[Category: Thermotoga]]

OCA: Protected "5bza" [edit=sysop:move=sysop]

OCA — Wed, 17 Jun 2015 14:06:30 GMT

Protected "5bza" [edit=sysop:move=sysop]

←Older revision

Revision as of 14:06, 17 June 2015

OCA: New page: '''Unreleased structure''' The entry 5bza is ON HOLD Authors: Ha, N.C., Kim, J.S., Yoon, B.Y. Description: Crystal structure of CbsA from Thermotoga neapolitana [[Category: Unreleased ...

OCA — Wed, 17 Jun 2015 14:06:28 GMT

New page: '''Unreleased structure''' The entry 5bza is ON HOLD Authors: Ha, N.C., Kim, J.S., Yoon, B.Y. Description: Crystal structure of CbsA from Thermotoga neapolitana [[Category: Unreleased ...

New page

'''Unreleased structure'''

The entry 5bza is ON HOLD

Authors: Ha, N.C., Kim, J.S., Yoon, B.Y.

Description: Crystal structure of CbsA from Thermotoga neapolitana
[[Category: Unreleased Structures]]
[[Category: Kim, J.S]]
[[Category: Yoon, B.Y]]
[[Category: Ha, N.C]]