5c4g - Revision history

OCA at 08:34, 27 September 2023

OCA — Wed, 27 Sep 2023 08:34:38 GMT

←Older revision		Revision as of 08:34, 27 September 2023
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5c4g]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5C4G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5C4G FirstGlance]. <br>		<table><tr><td colspan='2'>[[5c4g]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5C4G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5C4G FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BQR:N~2~-[7-(3,4-DIMETHOXYPHENYL)QUINOXALIN-2-YL]-N-METHYLGLYCINAMIDE'>BQR</scene>, <scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.2Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BQR:N~2~-[7-(3,4-DIMETHOXYPHENYL)QUINOXALIN-2-YL]-N-METHYLGLYCINAMIDE'>BQR</scene>, <scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5c4g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5c4g OCA], [https://pdbe.org/5c4g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5c4g RCSB], [https://www.ebi.ac.uk/pdbsum/5c4g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5c4g ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5c4g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5c4g OCA], [https://pdbe.org/5c4g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5c4g RCSB], [https://www.ebi.ac.uk/pdbsum/5c4g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5c4g ProSAT]</span></td></tr>
	</table>		</table>

OCA at 06:19, 7 June 2023

OCA — Wed, 07 Jun 2023 06:19:31 GMT

←Older revision		Revision as of 06:19, 7 June 2023
Line 3:		Line 3:
	<StructureSection load='5c4g' size='340' side='right'caption='[[5c4g]], [[Resolution\|resolution]] 3.20Å' scene=''>		<StructureSection load='5c4g' size='340' side='right'caption='[[5c4g]], [[Resolution\|resolution]] 3.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5c4g]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5C4G OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5C4G FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5c4g]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5C4G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5C4G FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BQR:N~2~-[7-(3,4-DIMETHOXYPHENYL)QUINOXALIN-2-YL]-N-METHYLGLYCINAMIDE'>BQR</scene>, <scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene~~></td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BQR:N~2~-[7-(3,4-DIMETHOXYPHENYL)QUINOXALIN-2-YL]-N-METHYLGLYCINAMIDE'>BQR</scene>, <scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">RAB11A, RAB11 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), PI4KB, PIK4CB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5c4g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5c4g OCA], [https://pdbe.org/5c4g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5c4g RCSB], [https://www.ebi.ac.uk/pdbsum/5c4g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5c4g ProSAT]</span></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/1-phosphatidylinositol_4-kinase 1-phosphatidylinositol 4-kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.67 2.7.1.67] </span></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5c4g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5c4g OCA], [~~http~~://pdbe.org/5c4g PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5c4g RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5c4g PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5c4g ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/RB11A_HUMAN RB11A_HUMAN]] The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab regulates endocytic recycling. Acts as a major regulator of membrane delivery during cytokinesis. Together with MYO5B and RAB8A participates in epithelial cell polarization. Together with RAB3IP, RAB8A, the exocyst complex, PARD3, PRKCI, ANXA2, CDC42 and DNMBP promotes transcytosis of PODXL to the apical membrane initiation sites (AMIS), apical surface formation and lumenogenesis. Together with MYO5B participates in CFTR trafficking to the plasma membrane and TF (Transferrin) recycling in nonpolarized cells. Required in a complex with MYO5B and RAB11FIP2 for the transport of NPC1L1 to the plasma membrane. Participates in the sorting and basolateral transport of CDH1 from the Golgi apparatus to the plasma membrane. Regulates the recycling of FCGRT (receptor of Fc region of monomeric Ig G) to basolateral membranes. May also play a role in melanosome transport and release from melanocytes.<ref>PMID:15601896</ref> <ref>PMID:15689490</ref> <ref>PMID:17462998</ref> <ref>PMID:19542231</ref> <ref>PMID:20890297</ref> <ref>PMID:21282656</ref> [[http://www.uniprot.org/uniprot/PI4KB_HUMAN PI4KB_HUMAN]] Phosphorylates phosphatidylinositol (PI) in the first committed step in the production of the second messenger inositol-1,4,5,-trisphosphate (PIP). May regulate Golgi disintegration/reorganization during mitosis, possibly via its phosphorylation. Involved in Golgi-to-plasma membrane trafficking (By similarity).<ref>PMID:9405935</ref> <ref>PMID:10559940</ref> <ref>PMID:12749687</ref>	+	[https://www.uniprot.org/uniprot/RB11A_HUMAN RB11A_HUMAN] The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab regulates endocytic recycling. Acts as a major regulator of membrane delivery during cytokinesis. Together with MYO5B and RAB8A participates in epithelial cell polarization. Together with RAB3IP, RAB8A, the exocyst complex, PARD3, PRKCI, ANXA2, CDC42 and DNMBP promotes transcytosis of PODXL to the apical membrane initiation sites (AMIS), apical surface formation and lumenogenesis. Together with MYO5B participates in CFTR trafficking to the plasma membrane and TF (Transferrin) recycling in nonpolarized cells. Required in a complex with MYO5B and RAB11FIP2 for the transport of NPC1L1 to the plasma membrane. Participates in the sorting and basolateral transport of CDH1 from the Golgi apparatus to the plasma membrane. Regulates the recycling of FCGRT (receptor of Fc region of monomeric Ig G) to basolateral membranes. May also play a role in melanosome transport and release from melanocytes.<ref>PMID:15601896</ref> <ref>PMID:15689490</ref> <ref>PMID:17462998</ref> <ref>PMID:19542231</ref> <ref>PMID:20890297</ref> <ref>PMID:21282656</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 22:		Line 20:

	==See Also==		==See Also==
-	*[[Ras-related protein Rab\|Ras-related protein Rab]]	+	*[[Phosphatidylinositol 4-kinase\|Phosphatidylinositol 4-kinase]]
		+	*[[Ras-related protein Rab 3D structures\|Ras-related protein Rab 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~1-phosphatidylinositol 4-kinase]]~~	+	[[Category: Homo sapiens]]
-	~~[[Category: Human~~]]	+
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Burke~~, J E~~]]	+	[[Category: Burke JE]]
-	[[Category: Fowler~~, M L]]~~	+	[[Category: Fowler ML]]
-	~~[[Category: Gtpase complex]]~~	+
-	~~[[Category: Lipid kinase]]~~	+
-	~~[[Category: Protein-protein complex]]~~	+
-	~~[[Category: Transferase-signaling protein complex~~]]	+

OCA at 07:58, 8 January 2020

OCA — Wed, 08 Jan 2020 07:58:21 GMT

←Older revision		Revision as of 07:58, 8 January 2020
Line 1:		Line 1:

	==Crystal structure of an engineered construct of phosphatidylinositol 4 kinase III beta with the inhibitor BQR695 in complex with GDP loaded Rab11==		==Crystal structure of an engineered construct of phosphatidylinositol 4 kinase III beta with the inhibitor BQR695 in complex with GDP loaded Rab11==
-	<StructureSection load='5c4g' size='340' side='right' caption='[[5c4g]], [[Resolution\|resolution]] 3.20Å' scene=''>	+	<StructureSection load='5c4g' size='340' side='right'caption='[[5c4g]], [[Resolution\|resolution]] 3.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5c4g]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5C4G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5C4G FirstGlance]. <br>		<table><tr><td colspan='2'>[[5c4g]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5C4G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5C4G FirstGlance]. <br>
Line 20:		Line 20:
	</div>		</div>
	<div class="pdbe-citations 5c4g" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5c4g" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Ras-related protein Rab\|Ras-related protein Rab]]
	== References ==		== References ==
	<references/>		<references/>
Line 26:		Line 29:
	[[Category: 1-phosphatidylinositol 4-kinase]]		[[Category: 1-phosphatidylinositol 4-kinase]]
	[[Category: Human]]		[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Burke, J E]]		[[Category: Burke, J E]]
	[[Category: Fowler, M L]]		[[Category: Fowler, M L]]

OCA at 16:29, 15 November 2017

OCA — Wed, 15 Nov 2017 16:29:37 GMT

←Older revision		Revision as of 16:29, 15 November 2017
Line 3:		Line 3:
	<StructureSection load='5c4g' size='340' side='right' caption='[[5c4g]], [[Resolution\|resolution]] 3.20Å' scene=''>		<StructureSection load='5c4g' size='340' side='right' caption='[[5c4g]], [[Resolution\|resolution]] 3.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5c4g]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5C4G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5C4G FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5c4g]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5C4G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5C4G FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BQR:N~2~-[7-(3,4-DIMETHOXYPHENYL)QUINOXALIN-2-YL]-N-METHYLGLYCINAMIDE'>BQR</scene>, <scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BQR:N~2~-[7-(3,4-DIMETHOXYPHENYL)QUINOXALIN-2-YL]-N-METHYLGLYCINAMIDE'>BQR</scene>, <scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">RAB11A, RAB11 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), PI4KB, PIK4CB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/1-phosphatidylinositol_4-kinase 1-phosphatidylinositol 4-kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.67 2.7.1.67] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/1-phosphatidylinositol_4-kinase 1-phosphatidylinositol 4-kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.67 2.7.1.67] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5c4g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5c4g OCA], [http://pdbe.org/5c4g PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5c4g RCSB], [http://www.ebi.ac.uk/pdbsum/5c4g PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5c4g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5c4g OCA], [http://pdbe.org/5c4g PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5c4g RCSB], [http://www.ebi.ac.uk/pdbsum/5c4g PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5c4g ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 24:		Line 25:
	</StructureSection>		</StructureSection>
	[[Category: 1-phosphatidylinositol 4-kinase]]		[[Category: 1-phosphatidylinositol 4-kinase]]
		+	[[Category: Human]]
	[[Category: Burke, J E]]		[[Category: Burke, J E]]
	[[Category: Fowler, M L]]		[[Category: Fowler, M L]]

OCA at 19:13, 10 May 2016

OCA — Tue, 10 May 2016 19:13:57 GMT

←Older revision		Revision as of 19:13, 10 May 2016
Line 1:		Line 1:
		+
	==Crystal structure of an engineered construct of phosphatidylinositol 4 kinase III beta with the inhibitor BQR695 in complex with GDP loaded Rab11==		==Crystal structure of an engineered construct of phosphatidylinositol 4 kinase III beta with the inhibitor BQR695 in complex with GDP loaded Rab11==
	<StructureSection load='5c4g' size='340' side='right' caption='[[5c4g]], [[Resolution\|resolution]] 3.20Å' scene=''>		<StructureSection load='5c4g' size='340' side='right' caption='[[5c4g]], [[Resolution\|resolution]] 3.20Å' scene=''>

OCA at 17:06, 20 January 2016

OCA — Wed, 20 Jan 2016 17:06:23 GMT

←Older revision		Revision as of 17:06, 20 January 2016
Line 1:		Line 1:
-	'''~~Unreleased~~ structure'''	+	==Crystal structure of an engineered construct of phosphatidylinositol 4 kinase III beta with the inhibitor BQR695 in complex with GDP loaded Rab11==
		+	<StructureSection load='5c4g' size='340' side='right' caption='[[5c4g]], [[Resolution\|resolution]] 3.20Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5c4g]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5C4G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5C4G FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BQR:N~2~-[7-(3,4-DIMETHOXYPHENYL)QUINOXALIN-2-YL]-N-METHYLGLYCINAMIDE'>BQR</scene>, <scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/1-phosphatidylinositol_4-kinase 1-phosphatidylinositol 4-kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.67 2.7.1.67] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5c4g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5c4g OCA], [http://pdbe.org/5c4g PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5c4g RCSB], [http://www.ebi.ac.uk/pdbsum/5c4g PDBsum]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/RB11A_HUMAN RB11A_HUMAN]] The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab regulates endocytic recycling. Acts as a major regulator of membrane delivery during cytokinesis. Together with MYO5B and RAB8A participates in epithelial cell polarization. Together with RAB3IP, RAB8A, the exocyst complex, PARD3, PRKCI, ANXA2, CDC42 and DNMBP promotes transcytosis of PODXL to the apical membrane initiation sites (AMIS), apical surface formation and lumenogenesis. Together with MYO5B participates in CFTR trafficking to the plasma membrane and TF (Transferrin) recycling in nonpolarized cells. Required in a complex with MYO5B and RAB11FIP2 for the transport of NPC1L1 to the plasma membrane. Participates in the sorting and basolateral transport of CDH1 from the Golgi apparatus to the plasma membrane. Regulates the recycling of FCGRT (receptor of Fc region of monomeric Ig G) to basolateral membranes. May also play a role in melanosome transport and release from melanocytes.<ref>PMID:15601896</ref> <ref>PMID:15689490</ref> <ref>PMID:17462998</ref> <ref>PMID:19542231</ref> <ref>PMID:20890297</ref> <ref>PMID:21282656</ref> [[http://www.uniprot.org/uniprot/PI4KB_HUMAN PI4KB_HUMAN]] Phosphorylates phosphatidylinositol (PI) in the first committed step in the production of the second messenger inositol-1,4,5,-trisphosphate (PIP). May regulate Golgi disintegration/reorganization during mitosis, possibly via its phosphorylation. Involved in Golgi-to-plasma membrane trafficking (By similarity).<ref>PMID:9405935</ref> <ref>PMID:10559940</ref> <ref>PMID:12749687</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The ability of proteins to bind and interact with protein partners plays fundamental roles in many cellular contexts. X-ray crystallography has been a powerful approach to understand protein-protein interactions, however, a challenge in the crystallization of proteins and their complexes is the presence of intrinsically disordered regions. In this article we describe an application of hydrogen deuterium exchange mass spectrometry (HDX-MS) to identify dynamic regions within type III phosphatidylinositol 4 kinase beta (PI4KIIIbeta) in complex with the GTPase Rab11. This information was then used to design deletions that allowed for the production of diffraction quality crystals. Importantly we also used HDX-MS to verify that the new construct was properly folded, consistent with it being catalytically and functionally active. Structures of PI4KIIIbeta in an Apo state and bound to the potent inhibitor BQR695 in complex with both GTPgammaS and GDP loaded Rab11 were determined. This hybrid HDX-MS/crystallographic strategy revealed novel aspects of the PI4KIIIbeta-Rab11 complex, as well as the molecular mechanism of potency of a PI4K specific inhibitor (BQR695). This approach is widely applicable to protein-protein complexes, and is an excellent strategy to optimize constructs for high-resolution structural approaches. This article is protected by copyright. All rights reserved.

-	~~The entry 5c4g is ON HOLD until Paper Publication~~	+	Using hydrogen deuterium exchange mass spectrometry to engineer optimized constructs for crystallization of protein complexes: Case study of PI4KIIIbeta with Rab11.,Fowler ML, McPhail JA, Jenkins ML, Masson GR, Rutaganira FU, Shokat KM, Williams RL, Burke JE Protein Sci. 2016 Jan 12. doi: 10.1002/pro.2879. PMID:26756197<ref>PMID:26756197</ref>

-	~~Authors: Burke~~, J.E.~~, Fowler, M.L~~.	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~Description~~: ~~Crystal structure of an engineered construct of phosphatidylinositol 4 kinase III beta with the inhibitor BQR695 in complex with GDP loaded Rab11~~	+	<div class="pdbe-citations 5c4g" style="background-color:#fffaf0;"></div>
-	[[Category: ~~Unreleased Structures~~]]	+	== References ==
-	[[Category: Burke, J.E]]	+	<references/>
-	[[Category: Fowler, M.L]]	+	__TOC__
		+	</StructureSection>
		+	[[Category: 1-phosphatidylinositol 4-kinase]]
		+	[[Category: Burke, J E]]
		+	[[Category: Fowler, M L]]
		+	[[Category: Gtpase complex]]
		+	[[Category: Lipid kinase]]
		+	[[Category: Protein-protein complex]]
		+	[[Category: Transferase-signaling protein complex]]

OCA at 01:02, 1 December 2015

OCA — Tue, 01 Dec 2015 01:02:51 GMT

←Older revision		Revision as of 01:02, 1 December 2015
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5c4g is ON HOLD	+	The entry 5c4g is ON HOLD until Paper Publication

-	Authors: Burke, J.E., Fowler, M.F.	+	Authors: Burke, J.E., Fowler, M.L.

	Description: Crystal structure of an engineered construct of phosphatidylinositol 4 kinase III beta with the inhibitor BQR695 in complex with GDP loaded Rab11		Description: Crystal structure of an engineered construct of phosphatidylinositol 4 kinase III beta with the inhibitor BQR695 in complex with GDP loaded Rab11
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: Burke, J.E]]		[[Category: Burke, J.E]]
-	[[Category: Fowler, M.F]]	+	[[Category: Fowler, M.L]]

OCA: Protected "5c4g" [edit=sysop:move=sysop]

OCA — Wed, 24 Jun 2015 12:04:21 GMT

Protected "5c4g" [edit=sysop:move=sysop]

←Older revision

Revision as of 12:04, 24 June 2015

OCA: New page: '''Unreleased structure''' The entry 5c4g is ON HOLD Authors: Burke, J.E., Fowler, M.F. Description: Crystal structure of an engineered construct of phosphatidylinositol 4 kinase III b...

OCA — Wed, 24 Jun 2015 12:04:20 GMT

New page: '''Unreleased structure''' The entry 5c4g is ON HOLD Authors: Burke, J.E., Fowler, M.F. Description: Crystal structure of an engineered construct of phosphatidylinositol 4 kinase III b...

New page

'''Unreleased structure'''

The entry 5c4g is ON HOLD

Authors: Burke, J.E., Fowler, M.F.

Description: Crystal structure of an engineered construct of phosphatidylinositol 4 kinase III beta with the inhibitor BQR695 in complex with GDP loaded Rab11
[[Category: Unreleased Structures]]
[[Category: Burke, J.E]]
[[Category: Fowler, M.F]]