5c71 - Revision history

OCA at 14:31, 29 November 2023

OCA — Wed, 29 Nov 2023 14:31:19 GMT

←Older revision		Revision as of 14:31, 29 November 2023
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5c71]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Aspergillus_oryzae Aspergillus oryzae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5C71 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5C71 FirstGlance]. <br>		<table><tr><td colspan='2'>[[5c71]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Aspergillus_oryzae Aspergillus oryzae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5C71 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5C71 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CBW:(3BETA,5BETA,14BETA)-3-HYDROXY-11-OXOOLEAN-12-EN-29-OIC+ACID'>CBW</scene>, <scene name='pdbligand=GCU:D-GLUCURONIC+ACID'>GCU</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.62Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CBW:(3BETA,5BETA,14BETA)-3-HYDROXY-11-OXOOLEAN-12-EN-29-OIC+ACID'>CBW</scene>, <scene name='pdbligand=GCU:D-GLUCURONIC+ACID'>GCU</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5c71 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5c71 OCA], [https://pdbe.org/5c71 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5c71 RCSB], [https://www.ebi.ac.uk/pdbsum/5c71 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5c71 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5c71 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5c71 OCA], [https://pdbe.org/5c71 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5c71 RCSB], [https://www.ebi.ac.uk/pdbsum/5c71 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5c71 ProSAT]</span></td></tr>
	</table>		</table>

OCA at 06:07, 2 March 2023

OCA — Thu, 02 Mar 2023 06:07:34 GMT

←Older revision		Revision as of 06:07, 2 March 2023
Line 3:		Line 3:
	<StructureSection load='5c71' size='340' side='right'caption='[[5c71]], [[Resolution\|resolution]] 2.62Å' scene=''>		<StructureSection load='5c71' size='340' side='right'caption='[[5c71]], [[Resolution\|resolution]] 2.62Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5c71]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Aspoz Aspoz~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5C71 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5C71 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5c71]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Aspergillus_oryzae Aspergillus oryzae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5C71 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5C71 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CBW:(3BETA,5BETA,14BETA)-3-HYDROXY-11-OXOOLEAN-12-EN-29-OIC+ACID'>CBW</scene>, <scene name='pdbligand=GCU:D-GLUCURONIC+ACID'>GCU</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CBW:(3BETA,5BETA,14BETA)-3-HYDROXY-11-OXOOLEAN-12-EN-29-OIC+ACID'>CBW</scene>, <scene name='pdbligand=GCU:D-GLUCURONIC+ACID'>GCU</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5c70\|5c70]]</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5c71 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5c71 OCA], [https://pdbe.org/5c71 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5c71 RCSB], [https://www.ebi.ac.uk/pdbsum/5c71 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5c71 ProSAT]</span></td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Pgus ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5062 ASPOZ])~~</td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5c71 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5c71 OCA], [~~http~~://pdbe.org/5c71 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5c71 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5c71 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5c71 ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A7XS03_ASPOZ A7XS03_ASPOZ]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Glycoside hydrolases (GHs) have attracted special attention in research aimed at modifying natural products by partial removal of sugar moieties to manipulate their solubility and efficacy. However, these modifications are challenging to control because the low substrate specificity of most GHs often generates undesired by-products. We previously identified a GH2-type fungal beta-glucuronidase from Aspergillus oryzae (PGUS) exhibiting promiscuous substrate specificity in hydrolysis of triterpenoid saponins. Here, we present the PGUS structure, representing the first structure of a fungal beta-glucuronidase, and that of an inactive PGUS mutant in complex with the native substrate glycyrrhetic acid 3-O-mono-beta-glucuronide (GAMG). PGUS displayed a homotetramer structure with each monomer comprising three distinct domains: a sugar-binding, an immunoglobulin-like beta-sandwich, and a TIM barrel domain. Two catalytic residues, Glu(414) and Glu(505), acted as acid/base and nucleophile, respectively. Structural and mutational analyses indicated that the GAMG glycan moiety is recognized by polar interactions with nine residues (Asp(162), His(332), Asp(414), Tyr(469), Tyr(473), Asp(505), Arg(563), Asn(567), and Lys(569)) and that the aglycone moiety is recognized by aromatic stacking and by a pi interaction with the four aromatic residues Tyr(469), Phe(470), Trp(472), and Tyr(473) Finally, structure-guided mutagenesis to precisely manipulate PGUS substrate specificity in the biotransformation of glycyrrhizin into GAMG revealed that two amino acids, Ala(365) and Arg(563), are critical for substrate specificity. Moreover, we obtained several mutants with dramatically improved GAMG yield (>95%). Structural analysis suggested that modulating the interaction of beta-glucuronidase simultaneously toward glycan and aglycone moieties is critical for tuning its substrate specificity toward triterpenoid saponins.
		+
		+	Structure-guided engineering of the substrate specificity of a fungal beta-glucuronidase toward triterpenoid saponins.,Lv B, Sun H, Huang S, Feng X, Jiang T, Li C J Biol Chem. 2018 Jan 12;293(2):433-443. doi: 10.1074/jbc.M117.801910. Epub 2017 , Nov 16. PMID:29146597<ref>PMID:29146597</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5c71" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Glucuronisidase 3D structures\|Glucuronisidase 3D structures]]		*[[Glucuronisidase 3D structures\|Glucuronisidase 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Aspoz~~]]	+	[[Category: Aspergillus oryzae]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Huang, S]]	+	[[Category: Huang S]]
-	[[Category: Jiang, T]]	+	[[Category: Jiang T]]
-	[[Category: Li, C]]	+	[[Category: Li C]]
-	[[Category: Lv, B]]	+	[[Category: Lv B]]
-	[[Category: Sun~~, H L]]~~	+	[[Category: Sun HL]]
-	~~[[Category: Beta-glucuronidase]]~~	+
-	~~[[Category: Glycyrrhetinic acid monoglucuronide]]~~	+
-	~~[[Category: Hydrolase~~]]	+

OCA at 11:14, 18 March 2020

OCA — Wed, 18 Mar 2020 11:14:17 GMT

←Older revision		Revision as of 11:14, 18 March 2020
Line 1:		Line 1:

	==The structure of Aspergillus oryzae a-glucuronidase complexed with glycyrrhetinic acid monoglucuronide==		==The structure of Aspergillus oryzae a-glucuronidase complexed with glycyrrhetinic acid monoglucuronide==
-	<StructureSection load='5c71' size='340' side='right' caption='[[5c71]], [[Resolution\|resolution]] 2.62Å' scene=''>	+	<StructureSection load='5c71' size='340' side='right'caption='[[5c71]], [[Resolution\|resolution]] 2.62Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5c71]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5C71 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5C71 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5c71]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Aspoz Aspoz]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5C71 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5C71 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CBW:(3BETA,5BETA,14BETA)-3-HYDROXY-11-OXOOLEAN-12-EN-29-OIC+ACID'>CBW</scene>, <scene name='pdbligand=GCU:D-GLUCURONIC+ACID'>GCU</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CBW:(3BETA,5BETA,14BETA)-3-HYDROXY-11-OXOOLEAN-12-EN-29-OIC+ACID'>CBW</scene>, <scene name='pdbligand=GCU:D-GLUCURONIC+ACID'>GCU</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5c70\|5c70]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5c70\|5c70]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Pgus ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5062 ASPOZ])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5c71 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5c71 OCA], [http://pdbe.org/5c71 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5c71 RCSB], [http://www.ebi.ac.uk/pdbsum/5c71 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5c71 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5c71 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5c71 OCA], [http://pdbe.org/5c71 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5c71 RCSB], [http://www.ebi.ac.uk/pdbsum/5c71 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5c71 ProSAT]</span></td></tr>
	</table>		</table>
		+
		+	==See Also==
		+	*[[Glucuronisidase 3D structures\|Glucuronisidase 3D structures]]
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Aspoz]]
		+	[[Category: Large Structures]]
	[[Category: Huang, S]]		[[Category: Huang, S]]
	[[Category: Jiang, T]]		[[Category: Jiang, T]]

OCA at 10:19, 13 July 2016

OCA — Wed, 13 Jul 2016 10:19:28 GMT

←Older revision		Revision as of 10:19, 13 July 2016
Line 1:		Line 1:
-	'''Unreleased structure'''

-	The ~~entry~~ 5c71 is ~~ON HOLD until Paper Publication~~	+	==The structure of Aspergillus oryzae a-glucuronidase complexed with glycyrrhetinic acid monoglucuronide==
-		+	<StructureSection load='5c71' size='340' side='right' caption='[[5c71]], [[Resolution\|resolution]] 2.62Å' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5c71]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5C71 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5C71 FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CBW:(3BETA,5BETA,14BETA)-3-HYDROXY-11-OXOOLEAN-12-EN-29-OIC+ACID'>CBW</scene>, <scene name='pdbligand=GCU:D-GLUCURONIC+ACID'>GCU</scene></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5c70\|5c70]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5c71 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5c71 OCA], [http://pdbe.org/5c71 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5c71 RCSB], [http://www.ebi.ac.uk/pdbsum/5c71 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5c71 ProSAT]</span></td></tr>
		+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Huang, S]]
		+	[[Category: Jiang, T]]
		+	[[Category: Li, C]]
		+	[[Category: Lv, B]]
		+	[[Category: Sun, H L]]
		+	[[Category: Beta-glucuronidase]]
		+	[[Category: Glycyrrhetinic acid monoglucuronide]]
		+	[[Category: Hydrolase]]

OCA at 11:21, 15 May 2016

OCA — Sun, 15 May 2016 11:21:59 GMT

←Older revision		Revision as of 11:21, 15 May 2016
Line 3:		Line 3:
	The entry 5c71 is ON HOLD until Paper Publication		The entry 5c71 is ON HOLD until Paper Publication

-	Authors: ~~Sun, H.L., Lv, B., Huang, S., Li, C., Jiang, T.~~	+	Authors:

	Description:		Description:
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Jiang, T]]
-	[[Category: Huang, S]]
-	[[Category: Sun, H.L]]
-	[[Category: Li, C]]
-	[[Category: Lv, B]]

OCA at 10:22, 16 December 2015

OCA — Wed, 16 Dec 2015 10:22:40 GMT

←Older revision		Revision as of 10:22, 16 December 2015
Line 7:		Line 7:
	Description:		Description:
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Sun, H.L]]
	[[Category: Jiang, T]]		[[Category: Jiang, T]]
	[[Category: Huang, S]]		[[Category: Huang, S]]
		+	[[Category: Sun, H.L]]
	[[Category: Li, C]]		[[Category: Li, C]]
	[[Category: Lv, B]]		[[Category: Lv, B]]

OCA at 10:30, 20 August 2015

OCA — Thu, 20 Aug 2015 10:30:46 GMT

←Older revision		Revision as of 10:30, 20 August 2015
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5c71 is ON HOLD	+	The entry 5c71 is ON HOLD until Paper Publication

	Authors: Sun, H.L., Lv, B., Huang, S., Li, C., Jiang, T.		Authors: Sun, H.L., Lv, B., Huang, S., Li, C., Jiang, T.

OCA at 12:53, 12 August 2015

OCA — Wed, 12 Aug 2015 12:53:18 GMT

←Older revision		Revision as of 12:53, 12 August 2015
Line 5:		Line 5:
	Authors: Sun, H.L., Lv, B., Huang, S., Li, C., Jiang, T.		Authors: Sun, H.L., Lv, B., Huang, S., Li, C., Jiang, T.

-	Description: ~~The structure of Penicillium purpurogenum beta-glucuronidase complexed with glycyrrhetinic acid monoglucuronide~~	+	Description:
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: Sun, H.L]]		[[Category: Sun, H.L]]

OCA at 12:50, 22 July 2015

OCA — Wed, 22 Jul 2015 12:50:36 GMT

←Older revision		Revision as of 12:50, 22 July 2015
Line 3:		Line 3:
	The entry 5c71 is ON HOLD		The entry 5c71 is ON HOLD

-	Authors: ~~Han-Li~~ Sun, Bo Lv, ~~Shen~~ Huang, ~~Chun~~ Li, ~~Tao~~ Jiang	+	Authors: Sun, H.L., Lv, B., Huang, S., Li, C., Jiang, T.

	Description: The structure of Penicillium purpurogenum beta-glucuronidase complexed with glycyrrhetinic acid monoglucuronide		Description: The structure of Penicillium purpurogenum beta-glucuronidase complexed with glycyrrhetinic acid monoglucuronide
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: ~~Han-Li~~ Sun, ~~Bo Lv~~, ~~Shen~~ Huang, ~~Chun~~ Li, ~~Tao Jiang~~]]	+	[[Category: Sun, H.L]]
		+	[[Category: Jiang, T]]
		+	[[Category: Huang, S]]
		+	[[Category: Li, C]]
		+	[[Category: Lv, B]]

OCA: Protected "5c71" [edit=sysop:move=sysop]

OCA — Wed, 01 Jul 2015 11:14:14 GMT

Protected "5c71" [edit=sysop:move=sysop]

←Older revision

Revision as of 11:14, 1 July 2015