5c9n - Revision history

OCA at 11:19, 10 January 2024

OCA — Wed, 10 Jan 2024 11:19:19 GMT

←Older revision		Revision as of 11:19, 10 January 2024
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5c9n]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5C9N OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5C9N FirstGlance]. <br>		<table><tr><td colspan='2'>[[5c9n]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5C9N OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5C9N FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.2Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5c9n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5c9n OCA], [https://pdbe.org/5c9n PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5c9n RCSB], [https://www.ebi.ac.uk/pdbsum/5c9n PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5c9n ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5c9n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5c9n OCA], [https://pdbe.org/5c9n PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5c9n RCSB], [https://www.ebi.ac.uk/pdbsum/5c9n PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5c9n ProSAT]</span></td></tr>
	</table>		</table>

OCA at 06:25, 7 June 2023

OCA — Wed, 07 Jun 2023 06:25:40 GMT

←Older revision		Revision as of 06:25, 7 June 2023
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	==Crystal structure of GEMC1 coiled-coil domain==		==Crystal structure of GEMC1 coiled-coil domain==
-	<StructureSection load='5c9n' size='340' side='right' caption='[[5c9n]], [[Resolution\|resolution]] 2.20Å' scene=''>	+	<StructureSection load='5c9n' size='340' side='right'caption='[[5c9n]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5c9n]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5C9N OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5C9N FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5c9n]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5C9N OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5C9N FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene></td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GMNC, GEMC1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5c9n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5c9n OCA], [https://pdbe.org/5c9n PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5c9n RCSB], [https://www.ebi.ac.uk/pdbsum/5c9n PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5c9n ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5c9n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5c9n OCA], [~~http~~://pdbe.org/5c9n PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5c9n RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5c9n PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5c9n ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/GEMC1_HUMAN GEMC1_HUMAN]] Regulator of DNA replication. Promotes initiation of chromosomal DNA replication by mediating TOPBP1- and CDK2-dependent recruitment of CDC45L onto replication origins (By similarity).	+	[https://www.uniprot.org/uniprot/GEMC1_HUMAN GEMC1_HUMAN] Regulator of DNA replication. Promotes initiation of chromosomal DNA replication by mediating TOPBP1- and CDK2-dependent recruitment of CDC45L onto replication origins (By similarity).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 23:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
-	[[Category: ~~Caillat, C~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Perrakis, A~~]]	+	[[Category: Caillat C]]
-	[[Category: ~~Cell cycle]]~~	+	[[Category: Perrakis A]]
-	~~[[Category: Geminin idas coiled coil dna replication license~~]]	+

OCA at 14:02, 16 November 2017

OCA — Thu, 16 Nov 2017 14:02:14 GMT

←Older revision		Revision as of 14:02, 16 November 2017
Line 1:		Line 1:
		+
	==Crystal structure of GEMC1 coiled-coil domain==		==Crystal structure of GEMC1 coiled-coil domain==
	<StructureSection load='5c9n' size='340' side='right' caption='[[5c9n]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='5c9n' size='340' side='right' caption='[[5c9n]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5c9n]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5C9N OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5C9N FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5c9n]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5C9N OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5C9N FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5c9n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5c9n OCA], [http://pdbe.org/5c9n PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5c9n RCSB], [http://www.ebi.ac.uk/pdbsum/5c9n PDBsum]</span></td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GMNC, GEMC1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5c9n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5c9n OCA], [http://pdbe.org/5c9n PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5c9n RCSB], [http://www.ebi.ac.uk/pdbsum/5c9n PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5c9n ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 21:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Human]]
	[[Category: Caillat, C]]		[[Category: Caillat, C]]
	[[Category: Perrakis, A]]		[[Category: Perrakis, A]]
	[[Category: Cell cycle]]		[[Category: Cell cycle]]
	[[Category: Geminin idas coiled coil dna replication license]]		[[Category: Geminin idas coiled coil dna replication license]]

OCA at 21:01, 30 November 2015

OCA — Mon, 30 Nov 2015 21:01:07 GMT

←Older revision		Revision as of 21:01, 30 November 2015
Line 1:		Line 1:
-	'''~~Unreleased~~ structure'''	+	==Crystal structure of GEMC1 coiled-coil domain==
		+	<StructureSection load='5c9n' size='340' side='right' caption='[[5c9n]], [[Resolution\|resolution]] 2.20Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5c9n]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5C9N OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5C9N FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5c9n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5c9n OCA], [http://pdbe.org/5c9n PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5c9n RCSB], [http://www.ebi.ac.uk/pdbsum/5c9n PDBsum]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/GEMC1_HUMAN GEMC1_HUMAN]] Regulator of DNA replication. Promotes initiation of chromosomal DNA replication by mediating TOPBP1- and CDK2-dependent recruitment of CDC45L onto replication origins (By similarity).
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	GemC1, together with Idas and Geminin, an important regulator of DNA-replication licensing and differentiation decisions, constitute a superfamily sharing a homologous central coiled-coil domain. To better understand this family of proteins, the crystal structure of a GemC1 coiled-coil domain variant engineered for better solubility was determined to 2.2 A resolution. GemC1 shows a less typical coiled coil compared with the Geminin homodimer and the Geminin-Idas heterodimer structures. It is also shown that both in vitro and in cells GemC1 interacts with Geminin through its coiled-coil domain, forming a heterodimer that is more stable that the GemC1 homodimer. Comparative analysis of the thermal stability of all of the possible superfamily complexes, using circular dichroism to follow the unfolding of the entire helix of the coiled coil, or intrinsic tryptophan fluorescence of a unique conserved N-terminal tryptophan, shows that the unfolding of the coiled coil is likely to take place from the C-terminus towards the N-terminus. It is also shown that homodimers show a single-state unfolding, while heterodimers show a two-state unfolding, suggesting that the dimer first falls apart and the helices then unfold according to the stability of each protein. The findings argue that Geminin-family members form homodimers and heterodimers between them, and this ability is likely to be important for modulating their function in cycling and differentiating cells.

-	The ~~entry 5c9n is ON HOLD~~	+	The structure of the GemC1 coiled coil and its interaction with the Geminin family of coiled-coil proteins.,Caillat C, Fish A, Pefani DE, Taraviras S, Lygerou Z, Perrakis A Acta Crystallogr D Biol Crystallogr. 2015 Nov 1;71(Pt 11):2278-86. doi:, 10.1107/S1399004715016892. Epub 2015 Oct 31. PMID:26527144<ref>PMID:26527144</ref>

-	~~Authors: Caillat~~, C.~~, Perrakis, A~~.	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~Description: Crystal structure of GEMC1 coiled~~-~~coil domain~~	+	<div class="pdbe-citations 5c9n" style="background-color:#fffaf0;"></div>
-	[[Category: ~~Unreleased Structures~~]]	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Caillat, C]]
	[[Category: Perrakis, A]]		[[Category: Perrakis, A]]
-	[[Category: ~~Caillat, C~~]]	+	[[Category: Cell cycle]]
		+	[[Category: Geminin idas coiled coil dna replication license]]

OCA: Protected "5c9n" [edit=sysop:move=sysop]

OCA — Wed, 08 Jul 2015 13:52:48 GMT

Protected "5c9n" [edit=sysop:move=sysop]

←Older revision

Revision as of 13:52, 8 July 2015

OCA: New page: '''Unreleased structure''' The entry 5c9n is ON HOLD Authors: Caillat, C., Perrakis, A. Description: Crystal structure of GEMC1 coiled-coil domain Category: Unreleased Structures [...

OCA — Wed, 08 Jul 2015 13:52:47 GMT

New page: '''Unreleased structure''' The entry 5c9n is ON HOLD Authors: Caillat, C., Perrakis, A. Description: Crystal structure of GEMC1 coiled-coil domain Category: Unreleased Structures [...

New page

'''Unreleased structure'''

The entry 5c9n is ON HOLD

Authors: Caillat, C., Perrakis, A.

Description: Crystal structure of GEMC1 coiled-coil domain
[[Category: Unreleased Structures]]
[[Category: Perrakis, A]]
[[Category: Caillat, C]]