5cht - Revision history

OCA at 11:20, 10 January 2024

OCA — Wed, 10 Jan 2024 11:20:26 GMT

←Older revision		Revision as of 11:20, 10 January 2024
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5cht]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5CHT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5CHT FirstGlance]. <br>		<table><tr><td colspan='2'>[[5cht]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5CHT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5CHT FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.8Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5cht FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5cht OCA], [https://pdbe.org/5cht PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5cht RCSB], [https://www.ebi.ac.uk/pdbsum/5cht PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5cht ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5cht FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5cht OCA], [https://pdbe.org/5cht PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5cht RCSB], [https://www.ebi.ac.uk/pdbsum/5cht PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5cht ProSAT]</span></td></tr>
	</table>		</table>

OCA at 12:07, 14 June 2023

OCA — Wed, 14 Jun 2023 12:07:12 GMT

←Older revision		Revision as of 12:07, 14 June 2023
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	<StructureSection load='5cht' size='340' side='right'caption='[[5cht]], [[Resolution\|resolution]] 2.80Å' scene=''>		<StructureSection load='5cht' size='340' side='right'caption='[[5cht]], [[Resolution\|resolution]] 2.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5cht]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Lk3_transgenic_mice Lk3 transgenic mice~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5CHT OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5CHT FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5cht]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5CHT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5CHT FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Usp18, Ubp43 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5cht FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5cht OCA], [https://pdbe.org/5cht PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5cht RCSB], [https://www.ebi.ac.uk/pdbsum/5cht PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5cht ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5cht FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5cht OCA], [~~http~~://pdbe.org/5cht PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5cht RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5cht PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5cht ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/UBP18_MOUSE UBP18_MOUSE]] Can efficiently cleave only ISG15 fusions including native ISG15 conjugates linked via isopeptide bonds. Necessary to maintain a critical cellular balance of ISG15-conjugated proteins in both healthy and stressed organisms (By similarity).	+	[https://www.uniprot.org/uniprot/UBP18_MOUSE UBP18_MOUSE] Can efficiently cleave only ISG15 fusions including native ISG15 conjugates linked via isopeptide bonds. Necessary to maintain a critical cellular balance of ISG15-conjugated proteins in both healthy and stressed organisms (By similarity).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 27:		Line 26:
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Lk3 transgenic mice~~]]	+	[[Category: Mus musculus]]
-	[[Category: Basters, A]]	+	[[Category: Basters A]]
-	[[Category: Fritz, G]]	+	[[Category: Fritz G]]
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Interferon]]~~	+
-	~~[[Category: Isg15]]~~	+
-	~~[[Category: Ubiquitin-specific protease~~]]	+

OCA at 11:39, 27 March 2020

OCA — Fri, 27 Mar 2020 11:39:09 GMT

←Older revision		Revision as of 11:39, 27 March 2020
Line 1:		Line 1:

	==Crystal structure of USP18==		==Crystal structure of USP18==
-	<StructureSection load='5cht' size='340' side='right' caption='[[5cht]], [[Resolution\|resolution]] 2.80Å' scene=''>	+	<StructureSection load='5cht' size='340' side='right'caption='[[5cht]], [[Resolution\|resolution]] 2.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5cht]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5CHT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5CHT FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5cht]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5CHT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5CHT FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Usp18, Ubp43 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5cht FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5cht OCA], [http://pdbe.org/5cht PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5cht RCSB], [http://www.ebi.ac.uk/pdbsum/5cht PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5cht ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5cht FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5cht OCA], [http://pdbe.org/5cht PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5cht RCSB], [http://www.ebi.ac.uk/pdbsum/5cht PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5cht ProSAT]</span></td></tr>
	</table>		</table>
Line 20:		Line 21:

	==See Also==		==See Also==
-	*[[Thioesterase\|Thioesterase]]	+	*[[Thioesterase 3D structures\|Thioesterase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Lk3 transgenic mice]]
	[[Category: Basters, A]]		[[Category: Basters, A]]
	[[Category: Fritz, G]]		[[Category: Fritz, G]]

OCA at 06:47, 9 March 2017

OCA — Thu, 09 Mar 2017 06:47:39 GMT

←Older revision		Revision as of 06:47, 9 March 2017
Line 9:		Line 9:
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/UBP18_MOUSE UBP18_MOUSE]] Can efficiently cleave only ISG15 fusions including native ISG15 conjugates linked via isopeptide bonds. Necessary to maintain a critical cellular balance of ISG15-conjugated proteins in both healthy and stressed organisms (By similarity).		[[http://www.uniprot.org/uniprot/UBP18_MOUSE UBP18_MOUSE]] Can efficiently cleave only ISG15 fusions including native ISG15 conjugates linked via isopeptide bonds. Necessary to maintain a critical cellular balance of ISG15-conjugated proteins in both healthy and stressed organisms (By similarity).
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Protein modification by ubiquitin and ubiquitin-like modifiers (Ubls) is counteracted by ubiquitin proteases and Ubl proteases, collectively termed DUBs. In contrast to other proteases of the ubiquitin-specific protease (USP) family, USP18 shows no reactivity toward ubiquitin but specifically deconjugates the interferon-induced Ubl ISG15. To identify the molecular determinants of this specificity, we solved the crystal structures of mouse USP18 alone and in complex with mouse ISG15. USP18 was crystallized in an open and a closed conformation, thus revealing high flexibility of the enzyme. Structural data, biochemical and mutational analysis showed that only the C-terminal ubiquitin-like domain of ISG15 is recognized and essential for USP18 activity. A critical hydrophobic patch in USP18 interacts with a hydrophobic region unique to ISG15, thus providing evidence that USP18's ISG15 specificity is mediated by a small interaction interface. Our results may provide a structural basis for the development of new drugs modulating ISG15 linkage.
		+
		+	Structural basis of the specificity of USP18 toward ISG15.,Basters A, Geurink PP, Rocker A, Witting KF, Tadayon R, Hess S, Semrau MS, Storici P, Ovaa H, Knobeloch KP, Fritz G Nat Struct Mol Biol. 2017 Mar;24(3):270-278. doi: 10.1038/nsmb.3371. Epub 2017, Feb 6. PMID:28165509<ref>PMID:28165509</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5cht" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Thioesterase\|Thioesterase]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 10:20, 13 July 2016

OCA — Wed, 13 Jul 2016 10:20:19 GMT

←Older revision		Revision as of 10:20, 13 July 2016
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5cht is ~~ON HOLD until Paper Publication~~	+	==Crystal structure of USP18==
-		+	<StructureSection load='5cht' size='340' side='right' caption='[[5cht]], [[Resolution\|resolution]] 2.80Å' scene=''>
-	~~Authors~~: ~~Fritz~~, G., ~~Basters~~, A.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5cht]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5CHT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5CHT FirstGlance]. <br>
-	~~Description~~: ~~Crystal structure~~ of ~~USP18~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5cht FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5cht OCA], [http://pdbe.org/5cht PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5cht RCSB], [http://www.ebi.ac.uk/pdbsum/5cht PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5cht ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/UBP18_MOUSE UBP18_MOUSE]] Can efficiently cleave only ISG15 fusions including native ISG15 conjugates linked via isopeptide bonds. Necessary to maintain a critical cellular balance of ISG15-conjugated proteins in both healthy and stressed organisms (By similarity).
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Basters, A]]
	[[Category: Fritz, G]]		[[Category: Fritz, G]]
-	[[Category: ~~Basters, A~~]]	+	[[Category: Hydrolase]]
		+	[[Category: Interferon]]
		+	[[Category: Isg15]]
		+	[[Category: Ubiquitin-specific protease]]

OCA at 18:34, 5 August 2015

OCA — Wed, 05 Aug 2015 18:34:59 GMT

←Older revision		Revision as of 18:34, 5 August 2015
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5cht is ON HOLD	+	The entry 5cht is ON HOLD until Paper Publication

	Authors: Fritz, G., Basters, A.		Authors: Fritz, G., Basters, A.

OCA: Protected "5cht" [edit=sysop:move=sysop]

OCA — Wed, 22 Jul 2015 13:08:51 GMT

Protected "5cht" [edit=sysop:move=sysop]

←Older revision

Revision as of 13:08, 22 July 2015

OCA: New page: '''Unreleased structure''' The entry 5cht is ON HOLD Authors: Fritz, G., Basters, A. Description: Crystal structure of USP18 Category: Unreleased Structures Category: Fritz, G ...

OCA — Wed, 22 Jul 2015 13:08:49 GMT

New page: '''Unreleased structure''' The entry 5cht is ON HOLD Authors: Fritz, G., Basters, A. Description: Crystal structure of USP18 Category: Unreleased Structures Category: Fritz, G ...

New page

'''Unreleased structure'''

The entry 5cht is ON HOLD

Authors: Fritz, G., Basters, A.

Description: Crystal structure of USP18
[[Category: Unreleased Structures]]
[[Category: Fritz, G]]
[[Category: Basters, A]]