5ckx - Revision history

OCA at 11:20, 10 January 2024

OCA — Wed, 10 Jan 2024 11:20:59 GMT

←Older revision		Revision as of 11:20, 10 January 2024
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5ckx]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5CKX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5CKX FirstGlance]. <br>		<table><tr><td colspan='2'>[[5ckx]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5CKX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5CKX FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=PHE:PHENYLALANINE'>PHE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=TSA:8-HYDROXY-2-OXA-BICYCLO[3.3.1]NON-6-ENE-3,5-DICARBOXYLIC+ACID'>TSA</scene>, <scene name='pdbligand=TYR:TYROSINE'>TYR</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.7Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=PHE:PHENYLALANINE'>PHE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=TSA:8-HYDROXY-2-OXA-BICYCLO[3.3.1]NON-6-ENE-3,5-DICARBOXYLIC+ACID'>TSA</scene>, <scene name='pdbligand=TYR:TYROSINE'>TYR</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ckx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ckx OCA], [https://pdbe.org/5ckx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ckx RCSB], [https://www.ebi.ac.uk/pdbsum/5ckx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ckx ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ckx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ckx OCA], [https://pdbe.org/5ckx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ckx RCSB], [https://www.ebi.ac.uk/pdbsum/5ckx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ckx ProSAT]</span></td></tr>
	</table>		</table>

OCA at 12:12, 14 June 2023

OCA — Wed, 14 Jun 2023 12:12:38 GMT

←Older revision		Revision as of 12:12, 14 June 2023
Line 3:		Line 3:
	<StructureSection load='5ckx' size='340' side='right'caption='[[5ckx]], [[Resolution\|resolution]] 2.70Å' scene=''>		<StructureSection load='5ckx' size='340' side='right'caption='[[5ckx]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5ckx]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Myctu Myctu~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5CKX OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5CKX FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5ckx]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5CKX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5CKX FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=PHE:PHENYLALANINE'>PHE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=TSA:8-HYDROXY-2-OXA-BICYCLO[3.3.1]NON-6-ENE-3,5-DICARBOXYLIC+ACID'>TSA</scene>, <scene name='pdbligand=TYR:TYROSINE'>TYR</scene~~></td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=PHE:PHENYLALANINE'>PHE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=TSA:8-HYDROXY-2-OXA-BICYCLO[3.3.1]NON-6-ENE-3,5-DICARBOXYLIC+ACID'>TSA</scene>, <scene name='pdbligand=TYR:TYROSINE'>TYR</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">aroG, Rv2178c ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83332 MYCTU]), Rv0948c, MTCY10D7.26 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83332 MYCTU])</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ckx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ckx OCA], [https://pdbe.org/5ckx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ckx RCSB], [https://www.ebi.ac.uk/pdbsum/5ckx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ckx ProSAT]</span></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/3-deoxy-7-phosphoheptulonate_synthase 3-deoxy-7-phosphoheptulonate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.54 2.5.1.54] </span></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5ckx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ckx OCA], [~~http~~://pdbe.org/5ckx PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5ckx RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5ckx PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5ckx ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/AROG_MYCTU AROG_MYCTU]] Catalyzes an aldol-like condensation reaction between phosphoenolpyruvate (PEP) and D-erythrose 4-phosphate (E4P) to generate 3-deoxy-D-arabino-heptulosonate 7-phosphate (DAH7P) and inorganic phosphate.<ref>PMID:16288916</ref> [[http://www.uniprot.org/uniprot/CHMU_MYCTU CHMU_MYCTU]] Catalyzes the Claisen rearrangement of chorismate to prephenate. Probably involved in the aromatic amino acid biosynthesis.<ref>PMID:15737998</ref> <ref>PMID:18727669</ref> <ref>PMID:19556970</ref>	+	[https://www.uniprot.org/uniprot/AROG_MYCTU AROG_MYCTU] Catalyzes an aldol-like condensation reaction between phosphoenolpyruvate (PEP) and D-erythrose 4-phosphate (E4P) to generate 3-deoxy-D-arabino-heptulosonate 7-phosphate (DAH7P) and inorganic phosphate.<ref>PMID:16288916</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 23:		Line 21:
	==See Also==		==See Also==
	*[[DAHP synthase 3D structures\|DAHP synthase 3D structures]]		*[[DAHP synthase 3D structures\|DAHP synthase 3D structures]]
		+	*[[3D structures of chorismate mutase\|3D structures of chorismate mutase]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: 3-deoxy-7-phosphoheptulonate synthase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Myctu~~]]	+	[[Category: Mycobacterium tuberculosis H37Rv]]
-	[[Category: Krengel, U]]	+	[[Category: Krengel U]]
-	[[Category: Munack, S]]	+	[[Category: Munack S]]
-	[[Category: Okvist, M]]	+	[[Category: Okvist M]]
-	~~[[Category: Chorismate mutase]]~~	+
-	~~[[Category: Dahp-synthase]]~~	+
-	~~[[Category: Protein complex]]~~	+
-	~~[[Category: Shikimate pathway]]~~	+
-	~~[[Category: Transferase-isomerase complex~~]]	+

OCA at 11:43, 27 March 2020

OCA — Fri, 27 Mar 2020 11:43:20 GMT

←Older revision		Revision as of 11:43, 27 March 2020
Line 1:		Line 1:

	==Non-covalent complex of DAHP synthase and chorismate mutase from Mycobacterium tuberculosis with bound transition state analog and feedback effectors tyrosine and phenylalanine==		==Non-covalent complex of DAHP synthase and chorismate mutase from Mycobacterium tuberculosis with bound transition state analog and feedback effectors tyrosine and phenylalanine==
-	<StructureSection load='5ckx' size='340' side='right' caption='[[5ckx]], [[Resolution\|resolution]] 2.70Å' scene=''>	+	<StructureSection load='5ckx' size='340' side='right'caption='[[5ckx]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5ckx]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5CKX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5CKX FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5ckx]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Myctu Myctu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5CKX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5CKX FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=PHE:PHENYLALANINE'>PHE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=TSA:8-HYDROXY-2-OXA-BICYCLO[3.3.1]NON-6-ENE-3,5-DICARBOXYLIC+ACID'>TSA</scene>, <scene name='pdbligand=TYR:TYROSINE'>TYR</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=PHE:PHENYLALANINE'>PHE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=TSA:8-HYDROXY-2-OXA-BICYCLO[3.3.1]NON-6-ENE-3,5-DICARBOXYLIC+ACID'>TSA</scene>, <scene name='pdbligand=TYR:TYROSINE'>TYR</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">aroG, Rv2178c ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83332 MYCTU]), Rv0948c, MTCY10D7.26 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83332 MYCTU])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/3-deoxy-7-phosphoheptulonate_synthase 3-deoxy-7-phosphoheptulonate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.54 2.5.1.54] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/3-deoxy-7-phosphoheptulonate_synthase 3-deoxy-7-phosphoheptulonate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.54 2.5.1.54] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ckx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ckx OCA], [http://pdbe.org/5ckx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ckx RCSB], [http://www.ebi.ac.uk/pdbsum/5ckx PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ckx ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ckx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ckx OCA], [http://pdbe.org/5ckx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ckx RCSB], [http://www.ebi.ac.uk/pdbsum/5ckx PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ckx ProSAT]</span></td></tr>
Line 19:		Line 20:
	</div>		</div>
	<div class="pdbe-citations 5ckx" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5ckx" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[DAHP synthase 3D structures\|DAHP synthase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 24:		Line 28:
	</StructureSection>		</StructureSection>
	[[Category: 3-deoxy-7-phosphoheptulonate synthase]]		[[Category: 3-deoxy-7-phosphoheptulonate synthase]]
		+	[[Category: Large Structures]]
		+	[[Category: Myctu]]
	[[Category: Krengel, U]]		[[Category: Krengel, U]]
	[[Category: Munack, S]]		[[Category: Munack, S]]

OCA at 06:39, 26 July 2016

OCA — Tue, 26 Jul 2016 06:39:53 GMT

←Older revision		Revision as of 06:39, 26 July 2016
Line 6:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=PHE:PHENYLALANINE'>PHE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=TSA:8-HYDROXY-2-OXA-BICYCLO[3.3.1]NON-6-ENE-3,5-DICARBOXYLIC+ACID'>TSA</scene>, <scene name='pdbligand=TYR:TYROSINE'>TYR</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=PHE:PHENYLALANINE'>PHE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=TSA:8-HYDROXY-2-OXA-BICYCLO[3.3.1]NON-6-ENE-3,5-DICARBOXYLIC+ACID'>TSA</scene>, <scene name='pdbligand=TYR:TYROSINE'>TYR</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/3-deoxy-7-phosphoheptulonate_synthase 3-deoxy-7-phosphoheptulonate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.54 2.5.1.54] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/3-deoxy-7-phosphoheptulonate_synthase 3-deoxy-7-phosphoheptulonate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.54 2.5.1.54] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ckx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ckx OCA], [http://pdbe.org/5ckx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ckx RCSB], [http://www.ebi.ac.uk/pdbsum/5ckx PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ckx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ckx OCA], [http://pdbe.org/5ckx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ckx RCSB], [http://www.ebi.ac.uk/pdbsum/5ckx PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ckx ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/AROG_MYCTU AROG_MYCTU]] Catalyzes an aldol-like condensation reaction between phosphoenolpyruvate (PEP) and D-erythrose 4-phosphate (E4P) to generate 3-deoxy-D-arabino-heptulosonate 7-phosphate (DAH7P) and inorganic phosphate.<ref>PMID:16288916</ref> [[http://www.uniprot.org/uniprot/CHMU_MYCTU CHMU_MYCTU]] Catalyzes the Claisen rearrangement of chorismate to prephenate. Probably involved in the aromatic amino acid biosynthesis.<ref>PMID:15737998</ref> <ref>PMID:18727669</ref> <ref>PMID:19556970</ref>		[[http://www.uniprot.org/uniprot/AROG_MYCTU AROG_MYCTU]] Catalyzes an aldol-like condensation reaction between phosphoenolpyruvate (PEP) and D-erythrose 4-phosphate (E4P) to generate 3-deoxy-D-arabino-heptulosonate 7-phosphate (DAH7P) and inorganic phosphate.<ref>PMID:16288916</ref> [[http://www.uniprot.org/uniprot/CHMU_MYCTU CHMU_MYCTU]] Catalyzes the Claisen rearrangement of chorismate to prephenate. Probably involved in the aromatic amino acid biosynthesis.<ref>PMID:15737998</ref> <ref>PMID:18727669</ref> <ref>PMID:19556970</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	DAHP synthase and chorismate mutase catalyze key steps in the shikimate biosynthetic pathway en route to aromatic amino acids. In Mycobacterium tuberculosis, chorismate mutase (MtCM; Rv0948c), located at the branch point towards phenylalanine and tyrosine, has poor activity on its own. However, it is efficiently activated by the first enzyme of the pathway, DAHP synthase (MtDS; Rv2178c), through formation of a non-covalent MtCM-MtDS complex. Here, we show how MtDS serves as an allosteric platform for feedback regulation of both enzymes, using X-ray crystallography, SAXS, SEC, and MALS. Crystal structures of the fully inhibited MtDS and the allosterically down-regulated MtCM-MtDS complex, solved at 2.8 and 2.7 A, respectively, reveal how effector binding at the internal MtDS subunit interfaces regulates the activity of MtDS and MtCM. While binding of all three metabolic end products to MtDS shuts down the entire pathway, the binding of phenylalanine jointly with tyrosine releases MtCM from the MtCM-MtDS complex, hence suppressing MtCM activation by 'inter-enzyme allostery'. This elegant regulatory principle, invoking a transient allosteric enzyme interaction, seems to be driven by dynamics and is likely a general strategy used by nature.
		+
		+	Remote Control by Inter-Enzyme Allostery: A Novel Paradigm for Regulation of the Shikimate Pathway.,Munack S, Roderer K, Okvist M, Kamarauskaite J, Sasso S, van Eerde A, Kast P, Krengel U J Mol Biol. 2016 Jan 8. pii: S0022-2836(16)00020-6. doi:, 10.1016/j.jmb.2016.01.001. PMID:26776476<ref>PMID:26776476</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5ckx" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 03:46, 11 May 2016

OCA — Wed, 11 May 2016 03:46:53 GMT

←Older revision		Revision as of 03:46, 11 May 2016
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry 5ckx is ON HOLD until Paper Publication~~	+	==Non-covalent complex of DAHP synthase and chorismate mutase from Mycobacterium tuberculosis with bound transition state analog and feedback effectors tyrosine and phenylalanine==
-		+	<StructureSection load='5ckx' size='340' side='right' caption='[[5ckx]], [[Resolution\|resolution]] 2.70Å' scene=''>
-	~~Authors: Munack, S., Okvist, M., Krengel, U.~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5ckx]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5CKX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5CKX FirstGlance]. <br>
-	~~Description:~~ Non-covalent complex of DAHP synthase and chorismate mutase from Mycobacterium tuberculosis with bound transition state analog and feedback effectors tyrosine and phenylalanine	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=PHE:PHENYLALANINE'>PHE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=TSA:8-HYDROXY-2-OXA-BICYCLO[3.3.1]NON-6-ENE-3,5-DICARBOXYLIC+ACID'>TSA</scene>, <scene name='pdbligand=TYR:TYROSINE'>TYR</scene></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/3-deoxy-7-phosphoheptulonate_synthase 3-deoxy-7-phosphoheptulonate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.54 2.5.1.54] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ckx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ckx OCA], [http://pdbe.org/5ckx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ckx RCSB], [http://www.ebi.ac.uk/pdbsum/5ckx PDBsum]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/AROG_MYCTU AROG_MYCTU]] Catalyzes an aldol-like condensation reaction between phosphoenolpyruvate (PEP) and D-erythrose 4-phosphate (E4P) to generate 3-deoxy-D-arabino-heptulosonate 7-phosphate (DAH7P) and inorganic phosphate.<ref>PMID:16288916</ref> [[http://www.uniprot.org/uniprot/CHMU_MYCTU CHMU_MYCTU]] Catalyzes the Claisen rearrangement of chorismate to prephenate. Probably involved in the aromatic amino acid biosynthesis.<ref>PMID:15737998</ref> <ref>PMID:18727669</ref> <ref>PMID:19556970</ref>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: 3-deoxy-7-phosphoheptulonate synthase]]
	[[Category: Krengel, U]]		[[Category: Krengel, U]]
	[[Category: Munack, S]]		[[Category: Munack, S]]
	[[Category: Okvist, M]]		[[Category: Okvist, M]]
		+	[[Category: Chorismate mutase]]
		+	[[Category: Dahp-synthase]]
		+	[[Category: Protein complex]]
		+	[[Category: Shikimate pathway]]
		+	[[Category: Transferase-isomerase complex]]

OCA at 22:24, 9 March 2016

OCA — Wed, 09 Mar 2016 22:24:40 GMT

←Older revision		Revision as of 22:24, 9 March 2016
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5ckx is ON HOLD	+	The entry 5ckx is ON HOLD until Paper Publication

	Authors: Munack, S., Okvist, M., Krengel, U.		Authors: Munack, S., Okvist, M., Krengel, U.

OCA at 21:37, 27 January 2016

OCA — Wed, 27 Jan 2016 21:37:57 GMT

←Older revision		Revision as of 21:37, 27 January 2016
Line 3:		Line 3:
	The entry 5ckx is ON HOLD		The entry 5ckx is ON HOLD

-	Authors: Munack, S., Okvist, M.	+	Authors: Munack, S., Okvist, M., Krengel, U.

	Description: Non-covalent complex of DAHP synthase and chorismate mutase from Mycobacterium tuberculosis with bound transition state analog and feedback effectors tyrosine and phenylalanine		Description: Non-covalent complex of DAHP synthase and chorismate mutase from Mycobacterium tuberculosis with bound transition state analog and feedback effectors tyrosine and phenylalanine
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
		+	[[Category: Krengel, U]]
	[[Category: Munack, S]]		[[Category: Munack, S]]
	[[Category: Okvist, M]]		[[Category: Okvist, M]]

OCA at 12:03, 20 January 2016

OCA — Wed, 20 Jan 2016 12:03:22 GMT

←Older revision		Revision as of 12:03, 20 January 2016
Line 5:		Line 5:
	Authors: Munack, S., Okvist, M.		Authors: Munack, S., Okvist, M.

-	Description: Non-covalent complex of DAHP-synthase and chorismate mutase from Mycobacterium tuberculosis	+	Description: Non-covalent complex of DAHP synthase and chorismate mutase from Mycobacterium tuberculosis with bound transition state analog and feedback effectors tyrosine and phenylalanine
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: Munack, S]]		[[Category: Munack, S]]
	[[Category: Okvist, M]]		[[Category: Okvist, M]]

OCA at 10:36, 16 December 2015

OCA — Wed, 16 Dec 2015 10:36:04 GMT

←Older revision		Revision as of 10:36, 16 December 2015
Line 7:		Line 7:
	Description: Non-covalent complex of DAHP-synthase and chorismate mutase from Mycobacterium tuberculosis		Description: Non-covalent complex of DAHP-synthase and chorismate mutase from Mycobacterium tuberculosis
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Okvist, M]]
	[[Category: Munack, S]]		[[Category: Munack, S]]
		+	[[Category: Okvist, M]]

OCA: Protected "5ckx" [edit=sysop:move=sysop]

OCA — Wed, 22 Jul 2015 13:16:40 GMT

Protected "5ckx" [edit=sysop:move=sysop]

←Older revision

Revision as of 13:16, 22 July 2015