5coc - Revision history

OCA at 16:10, 8 November 2023

OCA — Wed, 08 Nov 2023 16:10:41 GMT

←Older revision		Revision as of 16:10, 8 November 2023
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5coc]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5COC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5COC FirstGlance]. <br>		<table><tr><td colspan='2'>[[5coc]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5COC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5COC FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.6691Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5coc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5coc OCA], [https://pdbe.org/5coc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5coc RCSB], [https://www.ebi.ac.uk/pdbsum/5coc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5coc ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5coc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5coc OCA], [https://pdbe.org/5coc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5coc RCSB], [https://www.ebi.ac.uk/pdbsum/5coc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5coc ProSAT]</span></td></tr>
	</table>		</table>

OCA at 12:20, 14 June 2023

OCA — Wed, 14 Jun 2023 12:20:08 GMT

←Older revision		Revision as of 12:20, 14 June 2023
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	<StructureSection load='5coc' size='340' side='right'caption='[[5coc]], [[Resolution\|resolution]] 2.67Å' scene=''>		<StructureSection load='5coc' size='340' side='right'caption='[[5coc]], [[Resolution\|resolution]] 2.67Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5coc]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"micrococcus_aureus"_(rosenbach_1884)_zopf_1885 "micrococcus~~ aureus~~" (rosenbach 1884) zopf 1885~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5COC OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5COC FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5coc]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5COC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5COC FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CALM1, CALM, CAM, CAM1, CALM2, CAM2, CAMB, CALM3, CALML2, CAM3, CAMC, CAMIII ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1280 "Micrococcus aureus" (Rosenbach 1884) Zopf 1885])</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5coc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5coc OCA], [https://pdbe.org/5coc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5coc RCSB], [https://www.ebi.ac.uk/pdbsum/5coc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5coc ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5coc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5coc OCA], [~~http~~://pdbe.org/5coc PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5coc RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5coc PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5coc ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Disease ==
		+	[https://www.uniprot.org/uniprot/CALM1_HUMAN CALM1_HUMAN] The disease is caused by mutations affecting the gene represented in this entry. Mutations in CALM1 are the cause of CPVT4. The disease is caused by mutations affecting the gene represented in this entry. Mutations in CALM1 are the cause of LQT14.
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/SPA_STAAU SPA_STAAU] [https://www.uniprot.org/uniprot/CALM1_HUMAN CALM1_HUMAN] Calmodulin mediates the control of a large number of enzymes, ion channels, aquaporins and other proteins through calcium-binding. Among the enzymes to be stimulated by the calmodulin-calcium complex are a number of protein kinases and phosphatases. Together with CCP110 and centrin, is involved in a genetic pathway that regulates the centrosome cycle and progression through cytokinesis (PubMed:16760425). Mediates calcium-dependent inactivation of CACNA1C (PubMed:26969752). Positively regulates calcium-activated potassium channel activity of KCNN2 (PubMed:27165696).<ref>PMID:16760425</ref> <ref>PMID:23893133</ref> <ref>PMID:26969752</ref> <ref>PMID:27165696</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Jeong, W H~~]]	+	[[Category: Staphylococcus aureus]]
-	[[Category: ~~Lee, H~~]]	+	[[Category: Jeong WH]]
-	[[Category: Lee~~, J O]]~~	+	[[Category: Lee H]]
-	~~[[Category: Song, D~~ H]]	+	[[Category: Lee JO]]
-	[[Category: ~~Alpha helix~~]]	+	[[Category: Song DH]]
-	[[Category: ~~Cross-linker]]~~	+
-	~~[[Category: Fusion]]~~	+
-	~~[[Category: Protein binding~~]]	+

OCA at 11:47, 27 March 2020

OCA — Fri, 27 Mar 2020 11:47:25 GMT

←Older revision		Revision as of 11:47, 27 March 2020
Line 1:		Line 1:

	==Fusion protein of human calmodulin and B4 domain of protein A from staphylococcal aureus==		==Fusion protein of human calmodulin and B4 domain of protein A from staphylococcal aureus==
-	<StructureSection load='5coc' size='340' side='right' caption='[[5coc]], [[Resolution\|resolution]] 2.67Å' scene=''>	+	<StructureSection load='5coc' size='340' side='right'caption='[[5coc]], [[Resolution\|resolution]] 2.67Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5coc]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5COC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5COC FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5coc]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"micrococcus_aureus"_(rosenbach_1884)_zopf_1885 "micrococcus aureus" (rosenbach 1884) zopf 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5COC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5COC FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5coc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5coc OCA], [http://pdbe.org/5coc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5coc RCSB], [http://www.ebi.ac.uk/pdbsum/5coc PDBsum]</span></td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CALM1, CALM, CAM, CAM1, CALM2, CAM2, CAMB, CALM3, CALML2, CAM3, CAMC, CAMIII ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1280 "Micrococcus aureus" (Rosenbach 1884) Zopf 1885])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5coc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5coc OCA], [http://pdbe.org/5coc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5coc RCSB], [http://www.ebi.ac.uk/pdbsum/5coc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5coc ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 20:		Line 21:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Jeong, W H]]		[[Category: Jeong, W H]]
	[[Category: Lee, H]]		[[Category: Lee, H]]

OCA at 20:29, 11 May 2016

OCA — Wed, 11 May 2016 20:29:50 GMT

←Older revision		Revision as of 20:29, 11 May 2016
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5coc is ~~ON HOLD until Paper~~ Publication	+	==Fusion protein of human calmodulin and B4 domain of protein A from staphylococcal aureus==
		+	<StructureSection load='5coc' size='340' side='right' caption='[[5coc]], [[Resolution\|resolution]] 2.67Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5coc]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5COC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5COC FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5coc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5coc OCA], [http://pdbe.org/5coc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5coc RCSB], [http://www.ebi.ac.uk/pdbsum/5coc PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Building a sophisticated protein nano-assembly requires a method for linking protein components in a predictable and stable structure. Most of the cross linkers available have flexible spacers. Because of this, the linked hybrids have significant structural flexibility and the relative structure between their two components is largely unpredictable. Here we describe a method of connecting two proteins via a 'fusion alpha helix' formed by joining two pre-existing helices into a single extended helix. Because simple ligation of two helices does not guarantee the formation of a continuous helix, we used EY-CBS, a synthetic cross linker that has been shown to react selectively with cysteines in alpha-helices, to stabilize the connecting helix. Formation and stabilization of the fusion helix was confirmed by determining the crystal structures of the fusion proteins with and without bound EY-CBS. Our method should be widely applicable for linking protein building blocks to generate predictable structures.

-	~~Authors: Jeong, W.H~~., ~~Lee~~, H., Song, ~~D.H.~~, Lee, J.O.	+	Connecting two proteins using a fusion alpha helix stabilized by a chemical cross linker.,Jeong WH, Lee H, Song DH, Eom JH, Kim SC, Lee HS, Lee H, Lee JO Nat Commun. 2016 Mar 16;7:11031. doi: 10.1038/ncomms11031. PMID:26980593<ref>PMID:26980593</ref>

-	~~Description: Fusion protein~~ of ~~human calmodulin and B4 domain~~ of ~~protein A from staphylococcal aureus~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category~~: ~~Unreleased Structures]]~~	+	</div>
-	[[Category: ~~Song~~, D.H]]	+	<div class="pdbe-citations 5coc" style="background-color:#fffaf0;"></div>
-	~~[[Category: Lee, J.O~~]]	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Jeong, W H]]
	[[Category: Lee, H]]		[[Category: Lee, H]]
-	[[Category: ~~Jeong~~, W.H]]	+	[[Category: Lee, J O]]
		+	[[Category: Song, D H]]
		+	[[Category: Alpha helix]]
		+	[[Category: Cross-linker]]
		+	[[Category: Fusion]]
		+	[[Category: Protein binding]]

OCA at 10:56, 20 August 2015

OCA — Thu, 20 Aug 2015 10:56:20 GMT

←Older revision		Revision as of 10:56, 20 August 2015
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5coc is ON HOLD	+	The entry 5coc is ON HOLD until Paper Publication

	Authors: Jeong, W.H., Lee, H., Song, D.H., Lee, J.O.		Authors: Jeong, W.H., Lee, H., Song, D.H., Lee, J.O.

-	Description: Fusion protein of ~~mbp3-16~~ and B4 domain of protein A from staphylococcal aureus	+	Description: Fusion protein of human calmodulin and B4 domain of protein A from staphylococcal aureus
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: Song, D.H]]		[[Category: Song, D.H]]

OCA: Protected "5coc" [edit=sysop:move=sysop]

OCA — Wed, 29 Jul 2015 13:38:32 GMT

Protected "5coc" [edit=sysop:move=sysop]

←Older revision

Revision as of 13:38, 29 July 2015

OCA: New page: '''Unreleased structure''' The entry 5coc is ON HOLD Authors: Jeong, W.H., Lee, H., Song, D.H., Lee, J.O. Description: Fusion protein of mbp3-16 and B4 domain of protein A from staphyl...

OCA — Wed, 29 Jul 2015 13:38:31 GMT

New page: '''Unreleased structure''' The entry 5coc is ON HOLD Authors: Jeong, W.H., Lee, H., Song, D.H., Lee, J.O. Description: Fusion protein of mbp3-16 and B4 domain of protein A from staphyl...

New page

'''Unreleased structure'''

The entry 5coc is ON HOLD

Authors: Jeong, W.H., Lee, H., Song, D.H., Lee, J.O.

Description: Fusion protein of mbp3-16 and B4 domain of protein A from staphylococcal aureus
[[Category: Unreleased Structures]]
[[Category: Song, D.H]]
[[Category: Lee, J.O]]
[[Category: Lee, H]]
[[Category: Jeong, W.H]]