5cuk - Revision history

OCA at 12:21, 6 March 2024

OCA — Wed, 06 Mar 2024 12:21:38 GMT

←Older revision		Revision as of 12:21, 6 March 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5cuk]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5CUK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5CUK FirstGlance]. <br>		<table><tr><td colspan='2'>[[5cuk]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5CUK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5CUK FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.1Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5cuk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5cuk OCA], [https://pdbe.org/5cuk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5cuk RCSB], [https://www.ebi.ac.uk/pdbsum/5cuk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5cuk ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5cuk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5cuk OCA], [https://pdbe.org/5cuk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5cuk RCSB], [https://www.ebi.ac.uk/pdbsum/5cuk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5cuk ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/Q9I332_PSEAE Q9I332_PSEAE]		[https://www.uniprot.org/uniprot/Q9I332_PSEAE Q9I332_PSEAE]
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	The Type 3 Secretion System (T3SS) and the bacterial flagellum are related pathogenicity associated appendages found at the surface of many disease-causing bacteria. These appendages consist of long tubular structures that protrude away from the bacterial surface, to interact with the host cell and/or promote motility. A proposed "ruler" protein tightly regulates the length of both the T3SS and the flagellum, but the molecular basis for this length control has remained poorly characterized and controversial. Using the Pseudomonas aeruginosa T3SS as a model system, we report the first structure of a T3SS ruler protein, revealing a "ball-and-chain" architecture, with a globular C-terminal domain (the ball) preceded by a long intrinsically disordered N-terminal polypeptide chain. The dimensions and stability of the globular domain does not support its potential passage through the inner lumen of the T3SS needle. We further demonstrate that a conserved motif at the Nterminus of the ruler protein interacts with the T3SS autoprotease in the cytosolic side. Collectively, these data suggest a potential mechanism for needle length sensing by ruler proteins, whereby upon T3SS needle assembly the ruler protein's N-terminal end is anchored on the cytosolic side, with the globular domain located on the extracellular end of the growing needle. Sequence analysis of T3SS and flagellar ruler proteins show that this mechanism is likely conserved across systems.
-
-	The Structure of a T3SS Ruler Protein Suggests a Molecular Mechanism for Needle Length Sensing.,Bergeron JR, Fernandez L, Wasney GA, Vuckovic M, Reffuveille F, Hancock RE, Strynadka NC J Biol Chem. 2015 Nov 20. pii: jbc.M115.684423. PMID:26589798<ref>PMID:26589798</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 5cuk" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 12:28, 14 June 2023

OCA — Wed, 14 Jun 2023 12:28:27 GMT

←Older revision		Revision as of 12:28, 14 June 2023
Line 3:		Line 3:
	<StructureSection load='5cuk' size='340' side='right'caption='[[5cuk]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='5cuk' size='340' side='right'caption='[[5cuk]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5cuk]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Pseae Pseae~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5CUK OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5CUK FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5cuk]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5CUK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5CUK FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5cul\|5cul]]</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5cuk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5cuk OCA], [https://pdbe.org/5cuk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5cuk RCSB], [https://www.ebi.ac.uk/pdbsum/5cuk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5cuk ProSAT]</span></td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">pscP, PA1695 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=208964 PSEAE])~~</td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5cuk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5cuk OCA], [~~http~~://pdbe.org/5cuk PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5cuk RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5cuk PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5cuk ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q9I332_PSEAE Q9I332_PSEAE]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 23:		Line 23:
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Pseae~~]]	+	[[Category: Pseudomonas aeruginosa PAO1]]
-	[[Category: Bergeron~~, J R.C~~]]	+	[[Category: Bergeron JRC]]
-	[[Category: Strynadka~~, N C.J]]~~	+	[[Category: Strynadka NCJ]]
-	~~[[Category: Cell invasion]]~~	+
-	~~[[Category: Molecular ruler secretion system~~]]	+

OCA at 10:25, 1 April 2020

OCA — Wed, 01 Apr 2020 10:25:08 GMT

←Older revision		Revision as of 10:25, 1 April 2020
Line 1:		Line 1:
		+
	==Crystal structure of the PscP SS domain==		==Crystal structure of the PscP SS domain==
-	<StructureSection load='5cuk' size='340' side='right' caption='[[5cuk]], [[Resolution\|resolution]] 2.10Å' scene=''>	+	<StructureSection load='5cuk' size='340' side='right'caption='[[5cuk]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5cuk]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5CUK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5CUK FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5cuk]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseae Pseae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5CUK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5CUK FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5cul\|5cul]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5cul\|5cul]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5cuk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5cuk OCA], [http://pdbe.org/5cuk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5cuk RCSB], [http://www.ebi.ac.uk/pdbsum/5cuk PDBsum]</span></td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">pscP, PA1695 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=208964 PSEAE])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5cuk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5cuk OCA], [http://pdbe.org/5cuk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5cuk RCSB], [http://www.ebi.ac.uk/pdbsum/5cuk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5cuk ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 20:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Pseae]]
	[[Category: Bergeron, J R.C]]		[[Category: Bergeron, J R.C]]
	[[Category: Strynadka, N C.J]]		[[Category: Strynadka, N C.J]]
	[[Category: Cell invasion]]		[[Category: Cell invasion]]
	[[Category: Molecular ruler secretion system]]		[[Category: Molecular ruler secretion system]]

OCA at 16:10, 2 December 2015

OCA — Wed, 02 Dec 2015 16:10:01 GMT

←Older revision		Revision as of 16:10, 2 December 2015
Line 1:		Line 1:
-	'''~~Unreleased~~ structure'''	+	==Crystal structure of the PscP SS domain==
		+	<StructureSection load='5cuk' size='340' side='right' caption='[[5cuk]], [[Resolution\|resolution]] 2.10Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5cuk]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5CUK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5CUK FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5cul\|5cul]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5cuk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5cuk OCA], [http://pdbe.org/5cuk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5cuk RCSB], [http://www.ebi.ac.uk/pdbsum/5cuk PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The Type 3 Secretion System (T3SS) and the bacterial flagellum are related pathogenicity associated appendages found at the surface of many disease-causing bacteria. These appendages consist of long tubular structures that protrude away from the bacterial surface, to interact with the host cell and/or promote motility. A proposed "ruler" protein tightly regulates the length of both the T3SS and the flagellum, but the molecular basis for this length control has remained poorly characterized and controversial. Using the Pseudomonas aeruginosa T3SS as a model system, we report the first structure of a T3SS ruler protein, revealing a "ball-and-chain" architecture, with a globular C-terminal domain (the ball) preceded by a long intrinsically disordered N-terminal polypeptide chain. The dimensions and stability of the globular domain does not support its potential passage through the inner lumen of the T3SS needle. We further demonstrate that a conserved motif at the Nterminus of the ruler protein interacts with the T3SS autoprotease in the cytosolic side. Collectively, these data suggest a potential mechanism for needle length sensing by ruler proteins, whereby upon T3SS needle assembly the ruler protein's N-terminal end is anchored on the cytosolic side, with the globular domain located on the extracellular end of the growing needle. Sequence analysis of T3SS and flagellar ruler proteins show that this mechanism is likely conserved across systems.

-	The ~~entry 5cuk is ON HOLD until Paper Publication~~	+	The Structure of a T3SS Ruler Protein Suggests a Molecular Mechanism for Needle Length Sensing.,Bergeron JR, Fernandez L, Wasney GA, Vuckovic M, Reffuveille F, Hancock RE, Strynadka NC J Biol Chem. 2015 Nov 20. pii: jbc.M115.684423. PMID:26589798<ref>PMID:26589798</ref>

-	~~Authors: Bergeron~~, J.R.~~C., Strynadka, N.C.J~~.	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~Description~~: ~~Ruler protein~~	+	<div class="pdbe-citations 5cuk" style="background-color:#fffaf0;"></div>
-	~~[[Category: Unreleased Structures]]~~	+	== References ==
-	[[Category: Bergeron, J.R.C]]	+	<references/>
-	[[Category: Strynadka, N.C.J]]	+	__TOC__
		+	</StructureSection>
		+	[[Category: Bergeron, J R.C]]
		+	[[Category: Strynadka, N C.J]]
		+	[[Category: Cell invasion]]
		+	[[Category: Molecular ruler secretion system]]

OCA: Protected "5cuk" [edit=sysop:move=sysop]

OCA — Wed, 05 Aug 2015 18:57:57 GMT

Protected "5cuk" [edit=sysop:move=sysop]

←Older revision

Revision as of 18:57, 5 August 2015

OCA: New page: '''Unreleased structure''' The entry 5cuk is ON HOLD until Paper Publication Authors: Bergeron, J.R.C., Strynadka, N.C.J. Description: Ruler protein Category: Unreleased Structures...

OCA — Wed, 05 Aug 2015 18:57:56 GMT

New page: '''Unreleased structure''' The entry 5cuk is ON HOLD until Paper Publication Authors: Bergeron, J.R.C., Strynadka, N.C.J. Description: Ruler protein Category: Unreleased Structures...

New page

'''Unreleased structure'''

The entry 5cuk is ON HOLD until Paper Publication

Authors: Bergeron, J.R.C., Strynadka, N.C.J.

Description: Ruler protein
[[Category: Unreleased Structures]]
[[Category: Bergeron, J.R.C]]
[[Category: Strynadka, N.C.J]]