5cze - Revision history

OCA at 11:23, 10 January 2024

OCA — Wed, 10 Jan 2024 11:23:51 GMT

←Older revision		Revision as of 11:23, 10 January 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5cze]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_O157:H7_str._SS52 Escherichia coli O157:H7 str. SS52]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5CZE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5CZE FirstGlance]. <br>		<table><tr><td colspan='2'>[[5cze]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_O157:H7_str._SS52 Escherichia coli O157:H7 str. SS52]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5CZE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5CZE FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.82Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5cze FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5cze OCA], [https://pdbe.org/5cze PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5cze RCSB], [https://www.ebi.ac.uk/pdbsum/5cze PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5cze ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5cze FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5cze OCA], [https://pdbe.org/5cze PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5cze RCSB], [https://www.ebi.ac.uk/pdbsum/5cze PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5cze ProSAT]</span></td></tr>
	</table>		</table>

OCA at 10:15, 21 June 2023

OCA — Wed, 21 Jun 2023 10:15:16 GMT

←Older revision		Revision as of 10:15, 21 June 2023
Line 3:		Line 3:
	<StructureSection load='5cze' size='340' side='right'caption='[[5cze]], [[Resolution\|resolution]] 3.82Å' scene=''>		<StructureSection load='5cze' size='340' side='right'caption='[[5cze]], [[Resolution\|resolution]] 3.82Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5cze]] is a 8 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Escherichia_coli_o157~~:~~h7_str~~.~~_ss52~~ Escherichia coli ~~o157~~:h7 str. ~~ss52~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5CZE OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5CZE FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5cze]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_O157:H7_str._SS52 Escherichia coli O157:H7 str. SS52]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5CZE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5CZE FirstGlance]. <br>
-	</td></tr><tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SS52_2228 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1330457 Escherichia coli O157:H7 str. SS52]), relE2, AML07_29260, APZ14_24120, ARC77_01680, ERS085383_05243, ERS085404_05085, ERS150873_04952, JEONG1266_09605 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1330457 Escherichia coli O157:H7 str. SS52])</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5cze FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5cze OCA], [https://pdbe.org/5cze PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5cze RCSB], [https://www.ebi.ac.uk/pdbsum/5cze PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5cze ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5cze FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5cze OCA], [~~http~~://pdbe.org/5cze PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5cze RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5cze PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5cze ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A0A0D7C2L1_ECOLX A0A0D7C2L1_ECOLX]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 21:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Escherichia coli ~~o157~~:h7 str. ~~ss52~~]]	+	[[Category: Escherichia coli O157:H7 str. SS52]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Loris, R]]	+	[[Category: Loris R]]
-	[[Category: Sterckx~~, Y G.J]]~~	+	[[Category: Sterckx YGJ]]
-	~~[[Category: Toxin]]~~	+
-	~~[[Category: Toxin-antitoxin~~]]	+

OCA at 10:30, 1 April 2020

OCA — Wed, 01 Apr 2020 10:30:19 GMT

←Older revision		Revision as of 10:30, 1 April 2020
Line 1:		Line 1:

	==Crystal structure of the PaaA2-ParE2 antitoxin-toxin complex==		==Crystal structure of the PaaA2-ParE2 antitoxin-toxin complex==
-	<StructureSection load='5cze' size='340' side='right' caption='[[5cze]], [[Resolution\|resolution]] 3.82Å' scene=''>	+	<StructureSection load='5cze' size='340' side='right'caption='[[5cze]], [[Resolution\|resolution]] 3.82Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5cze]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5CZE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5CZE FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5cze]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_o157:h7_str._ss52 Escherichia coli o157:h7 str. ss52]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5CZE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5CZE FirstGlance]. <br>
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SS52_2228 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1330457 Escherichia coli O157:H7 str. SS52]), relE2, AML07_29260, APZ14_24120, ARC77_01680, ERS085383_05243, ERS085404_05085, ERS150873_04952, JEONG1266_09605 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1330457 Escherichia coli O157:H7 str. SS52])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5cze FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5cze OCA], [http://pdbe.org/5cze PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5cze RCSB], [http://www.ebi.ac.uk/pdbsum/5cze PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5cze ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5cze FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5cze OCA], [http://pdbe.org/5cze PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5cze RCSB], [http://www.ebi.ac.uk/pdbsum/5cze PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5cze ProSAT]</span></td></tr>
	</table>		</table>
Line 20:		Line 21:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Escherichia coli o157:h7 str. ss52]]
		+	[[Category: Large Structures]]
	[[Category: Loris, R]]		[[Category: Loris, R]]
	[[Category: Sterckx, Y G.J]]		[[Category: Sterckx, Y G.J]]
	[[Category: Toxin]]		[[Category: Toxin]]
	[[Category: Toxin-antitoxin]]		[[Category: Toxin-antitoxin]]

OCA at 06:49, 10 September 2016

OCA — Sat, 10 Sep 2016 06:49:11 GMT

←Older revision		Revision as of 06:49, 10 September 2016
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5cze is ~~ON HOLD~~	+	==Crystal structure of the PaaA2-ParE2 antitoxin-toxin complex==
		+	<StructureSection load='5cze' size='340' side='right' caption='[[5cze]], [[Resolution\|resolution]] 3.82Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5cze]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5CZE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5CZE FirstGlance]. <br>
		+	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5cze FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5cze OCA], [http://pdbe.org/5cze PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5cze RCSB], [http://www.ebi.ac.uk/pdbsum/5cze PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5cze ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Many bacterial pathogens modulate their metabolic activity, virulence and pathogenicity through so-called "toxin-antitoxin" (TA) modules. The genome of the human pathogen Escherichia coli O157 contains two three-component TA modules related to the known parDE module. Here, we show that the toxin EcParE2 maps in a branch of the RelE/ParE toxin superfamily that is distinct from the branches that contain verified gyrase and ribosome inhibitors. The structure of EcParE2 closely resembles that of Caulobacter crescentus ParE but shows a distinct pattern of conserved surface residues, in agreement with its apparent inability to interact with GyrA. The antitoxin EcPaaA2 is characterized by two alpha-helices (H1 and H2) that serve as molecular recognition elements to wrap itself around EcParE2. Both EcPaaA2 H1 and H2 are required to sustain a high-affinity interaction with EcParE2 and for the inhibition of EcParE2-mediated killing in vivo. Furthermore, evidence demonstrates that EcPaaA2 H2, but not H1, determines specificity for EcParE2. The initially formed EcPaaA2-EcParE2 heterodimer then assembles into a hetero-hexadecamer, which is stable in solution and is formed in a highly cooperative manner. Together these findings provide novel data on quaternary structure, TA interactions and activity of a hitherto poorly characterized family of TA modules.

-	~~Authors: Loris, R~~., Sterckx, Y.G.J.	+	A unique hetero-hexadecameric architecture displayed by the Escherichia coli O157 PaaA2-ParE2 antitoxin-toxin complex.,Sterckx YG, Jove T, Shkumatov AV, Garcia-Pino A, Geerts L, De Kerpel M, Lah J, De Greve H, Van Melderen L, Loris R J Mol Biol. 2016 Apr 24;428(8):1589-603. doi: 10.1016/j.jmb.2016.03.007. Epub, 2016 Mar 18. PMID:26996937<ref>PMID:26996937</ref>

-	~~Description: Crystal structure~~ of the ~~PaaA2~~-~~ParE2 antitoxin~~-~~toxin complex~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category~~: ~~Unreleased Structures]]~~	+	</div>
		+	<div class="pdbe-citations 5cze" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Loris, R]]		[[Category: Loris, R]]
-	[[Category: Sterckx, Y.G.J]]	+	[[Category: Sterckx, Y G.J]]
		+	[[Category: Toxin]]
		+	[[Category: Toxin-antitoxin]]

OCA at 10:02, 1 June 2016

OCA — Wed, 01 Jun 2016 10:02:24 GMT

←Older revision		Revision as of 10:02, 1 June 2016
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5cze is ON HOLD ~~until Paper Publication~~	+	The entry 5cze is ON HOLD

	Authors: Loris, R., Sterckx, Y.G.J.		Authors: Loris, R., Sterckx, Y.G.J.

OCA: Protected "5cze" [edit=sysop:move=sysop]

OCA — Wed, 12 Aug 2015 13:36:49 GMT

Protected "5cze" [edit=sysop:move=sysop]

←Older revision

Revision as of 13:36, 12 August 2015

OCA: New page: '''Unreleased structure''' The entry 5cze is ON HOLD until Paper Publication Authors: Loris, R., Sterckx, Y.G.J. Description: Crystal structure of the PaaA2-ParE2 antitoxin-toxin compl...

OCA — Wed, 12 Aug 2015 13:36:48 GMT

New page: '''Unreleased structure''' The entry 5cze is ON HOLD until Paper Publication Authors: Loris, R., Sterckx, Y.G.J. Description: Crystal structure of the PaaA2-ParE2 antitoxin-toxin compl...

New page

'''Unreleased structure'''

The entry 5cze is ON HOLD until Paper Publication

Authors: Loris, R., Sterckx, Y.G.J.

Description: Crystal structure of the PaaA2-ParE2 antitoxin-toxin complex
[[Category: Unreleased Structures]]
[[Category: Loris, R]]
[[Category: Sterckx, Y.G.J]]