5d5c - Revision history

OCA at 11:24, 10 January 2024

OCA — Wed, 10 Jan 2024 11:24:36 GMT

←Older revision		Revision as of 11:24, 10 January 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5d5c]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5D5C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5D5C FirstGlance]. <br>		<table><tr><td colspan='2'>[[5d5c]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5D5C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5D5C FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACY:ACETIC+ACID'>ACY</scene>, <scene name='pdbligand=BR:BROMIDE+ION'>BR</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PE5:3,6,9,12,15,18,21,24-OCTAOXAHEXACOSAN-1-OL'>PE5</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.7Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACY:ACETIC+ACID'>ACY</scene>, <scene name='pdbligand=BR:BROMIDE+ION'>BR</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PE5:3,6,9,12,15,18,21,24-OCTAOXAHEXACOSAN-1-OL'>PE5</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5d5c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5d5c OCA], [https://pdbe.org/5d5c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5d5c RCSB], [https://www.ebi.ac.uk/pdbsum/5d5c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5d5c ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5d5c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5d5c OCA], [https://pdbe.org/5d5c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5d5c RCSB], [https://www.ebi.ac.uk/pdbsum/5d5c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5d5c ProSAT]</span></td></tr>
	</table>		</table>

OCA at 10:22, 21 June 2023

OCA — Wed, 21 Jun 2023 10:22:30 GMT

←Older revision		Revision as of 10:22, 21 June 2023
Line 3:		Line 3:
	<StructureSection load='5d5c' size='340' side='right'caption='[[5d5c]], [[Resolution\|resolution]] 1.70Å' scene=''>		<StructureSection load='5d5c' size='340' side='right'caption='[[5d5c]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5d5c]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5D5C OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5D5C FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5d5c]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5D5C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5D5C FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACY:ACETIC+ACID'>ACY</scene>, <scene name='pdbligand=BR:BROMIDE+ION'>BR</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PE5:3,6,9,12,15,18,21,24-OCTAOXAHEXACOSAN-1-OL'>PE5</scene~~></td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACY:ACETIC+ACID'>ACY</scene>, <scene name='pdbligand=BR:BROMIDE+ION'>BR</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PE5:3,6,9,12,15,18,21,24-OCTAOXAHEXACOSAN-1-OL'>PE5</scene></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5d5c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5d5c OCA], [https://pdbe.org/5d5c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5d5c RCSB], [https://www.ebi.ac.uk/pdbsum/5d5c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5d5c ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5d5c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5d5c OCA], [~~http~~://pdbe.org/5d5c PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5d5c RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5d5c PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5d5c ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/LYSC_CHICK LYSC_CHICK]] Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus.<ref>PMID:22044478</ref>	+	[https://www.uniprot.org/uniprot/LYSC_CHICK LYSC_CHICK] Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus.<ref>PMID:22044478</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 28:		Line 27:
	[[Category: Gallus gallus]]		[[Category: Gallus gallus]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	~~[[Category: Lysozyme]]~~	+	[[Category: Caffrey M]]
-	[[Category: Caffrey, M]]	+	[[Category: Diederichs K]]
-	[[Category: Diederichs, K]]	+	[[Category: Huang C-Y]]
-	[[Category: Huang, C Y]]	+	[[Category: Olieric V]]
-	[[Category: Olieric, V]]	+	[[Category: Wang M]]
-	[[Category: Wang, M]]	+
-	~~[[Category: Hydrolase~~]]	+

OCA at 10:38, 1 April 2020

OCA — Wed, 01 Apr 2020 10:38:19 GMT

←Older revision		Revision as of 10:38, 1 April 2020
Line 1:		Line 1:

	==In meso in situ serial X-ray crystallography structure of lysozyme at 100 K==		==In meso in situ serial X-ray crystallography structure of lysozyme at 100 K==
-	<StructureSection load='5d5c' size='340' side='right' caption='[[5d5c]], [[Resolution\|resolution]] 1.70Å' scene=''>	+	<StructureSection load='5d5c' size='340' side='right'caption='[[5d5c]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5d5c]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5D5C OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5D5C FirstGlance]. <br>		<table><tr><td colspan='2'>[[5d5c]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5D5C OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5D5C FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACY:ACETIC+ACID'>ACY</scene>, <scene name='pdbligand=BR:BROMIDE+ION'>BR</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PE5:3,6,9,12,15,18,21,24-OCTAOXAHEXACOSAN-1-OL'>PE5</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACY:ACETIC+ACID'>ACY</scene>, <scene name='pdbligand=BR:BROMIDE+ION'>BR</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PE5:3,6,9,12,15,18,21,24-OCTAOXAHEXACOSAN-1-OL'>PE5</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5d5c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5d5c OCA], [http://pdbe.org/5d5c PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5d5c RCSB], [http://www.ebi.ac.uk/pdbsum/5d5c PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5d5c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5d5c OCA], [http://pdbe.org/5d5c PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5d5c RCSB], [http://www.ebi.ac.uk/pdbsum/5d5c PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5d5c ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 19:		Line 19:
	</div>		</div>
	<div class="pdbe-citations 5d5c" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5d5c" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Lysozyme 3D structures\|Lysozyme 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 24:		Line 27:
	</StructureSection>		</StructureSection>
	[[Category: Gallus gallus]]		[[Category: Gallus gallus]]
		+	[[Category: Large Structures]]
	[[Category: Lysozyme]]		[[Category: Lysozyme]]
	[[Category: Caffrey, M]]		[[Category: Caffrey, M]]

OCA at 07:04, 2 March 2016

OCA — Wed, 02 Mar 2016 07:04:45 GMT

←Older revision		Revision as of 07:04, 2 March 2016
Line 1:		Line 1:
		+
	==In meso in situ serial X-ray crystallography structure of lysozyme at 100 K==		==In meso in situ serial X-ray crystallography structure of lysozyme at 100 K==
	<StructureSection load='5d5c' size='340' side='right' caption='[[5d5c]], [[Resolution\|resolution]] 1.70Å' scene=''>		<StructureSection load='5d5c' size='340' side='right' caption='[[5d5c]], [[Resolution\|resolution]] 1.70Å' scene=''>
Line 9:		Line 10:
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/LYSC_CHICK LYSC_CHICK]] Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus.<ref>PMID:22044478</ref>		[[http://www.uniprot.org/uniprot/LYSC_CHICK LYSC_CHICK]] Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus.<ref>PMID:22044478</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Here, a method for presenting crystals of soluble and membrane proteins growing in the lipid cubic or sponge phase for in situ diffraction data collection at cryogenic temperatures is introduced. The method dispenses with the need for the technically demanding and inefficient crystal-harvesting step that is an integral part of the lipid cubic phase or in meso method of growing crystals. Crystals are dispersed in a bolus of mesophase sandwiched between thin plastic windows. The bolus contains tens to hundreds of crystals, visible with an in-line microscope at macromolecular crystallography synchrotron beamlines and suitably disposed for conventional or serial crystallographic data collection. Wells containing the crystal-laden boluses are removed individually from hermetically sealed glass plates in which crystallization occurs, affixed to pins on goniometer bases and excess precipitant is removed from around the mesophase. The wells are snap-cooled in liquid nitrogen, stored and shipped in Dewars, and manually or robotically mounted on a goniometer in a cryostream for diffraction data collection at 100 K, as is performed routinely with standard, loop-harvested crystals. The method is a variant on the recently introduced in meso in situ serial crystallography (IMISX) method that enables crystallographic measurements at cryogenic temperatures where crystal lifetimes are enormously enhanced whilst reducing protein consumption dramatically. The new approach has been used to generate high-resolution crystal structures of a G-protein-coupled receptor, alpha-helical and beta-barrel transporters and an enzyme as model integral membrane proteins. Insulin and lysozyme were used as test soluble proteins. The quality of the data that can be generated by this method was attested to by performing sulfur and bromine SAD phasing with two of the test proteins.
		+
		+	In meso in situ serial X-ray crystallography of soluble and membrane proteins at cryogenic temperatures.,Huang CY, Olieric V, Ma P, Howe N, Vogeley L, Liu X, Warshamanage R, Weinert T, Panepucci E, Kobilka B, Diederichs K, Wang M, Caffrey M Acta Crystallogr D Struct Biol. 2016 Jan;72(Pt 1):93-112. doi:, 10.1107/S2059798315021683. Epub 2016 Jan 1. PMID:26894538<ref>PMID:26894538</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5d5c" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 20:31, 13 January 2016

OCA — Wed, 13 Jan 2016 20:31:02 GMT

←Older revision		Revision as of 20:31, 13 January 2016
Line 1:		Line 1:
-	'''~~Unreleased structure~~'''	+	==In meso in situ serial X-ray crystallography structure of lysozyme at 100 K==
-		+	<StructureSection load='5d5c' size='340' side='right' caption='[[5d5c]], [[Resolution\|resolution]] 1.70Å' scene=''>
-	~~The entry~~ 5d5c is ~~ON HOLD until Paper Publication~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5d5c]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5D5C OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5D5C FirstGlance]. <br>
-	~~Authors~~: ~~Huang~~, C.-Y.	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACY:ACETIC+ACID'>ACY</scene>, <scene name='pdbligand=BR:BROMIDE+ION'>BR</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PE5:3,6,9,12,15,18,21,24-OCTAOXAHEXACOSAN-1-OL'>PE5</scene></td></tr>
-		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span></td></tr>
-	~~Description~~:	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5d5c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5d5c OCA], [http://pdbe.org/5d5c PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5d5c RCSB], [http://www.ebi.ac.uk/pdbsum/5d5c PDBsum]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
-	[[Category: Huang, C.-Y]]	+	== Function ==
		+	[[http://www.uniprot.org/uniprot/LYSC_CHICK LYSC_CHICK]] Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus.<ref>PMID:22044478</ref>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Gallus gallus]]
		+	[[Category: Lysozyme]]
		+	[[Category: Caffrey, M]]
		+	[[Category: Diederichs, K]]
		+	[[Category: Huang, C Y]]
		+	[[Category: Olieric, V]]
		+	[[Category: Wang, M]]
		+	[[Category: Hydrolase]]

OCA at 12:26, 26 August 2015

OCA — Wed, 26 Aug 2015 12:26:49 GMT

←Older revision		Revision as of 12:26, 26 August 2015
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5d5c is ON HOLD	+	The entry 5d5c is ON HOLD until Paper Publication

	Authors: Huang, C.-Y.		Authors: Huang, C.-Y.

OCA: Protected "5d5c" [edit=sysop:move=sysop]

OCA — Thu, 20 Aug 2015 11:23:23 GMT

Protected "5d5c" [edit=sysop:move=sysop]

←Older revision

Revision as of 11:23, 20 August 2015

OCA: New page: '''Unreleased structure''' The entry 5d5c is ON HOLD Authors: Huang, C.-Y. Description: Category: Unreleased Structures Category: Huang, C.-Y

OCA — Thu, 20 Aug 2015 11:23:21 GMT

New page: '''Unreleased structure''' The entry 5d5c is ON HOLD Authors: Huang, C.-Y. Description: Category: Unreleased Structures Category: Huang, C.-Y

New page

'''Unreleased structure'''

The entry 5d5c is ON HOLD

Authors: Huang, C.-Y.

Description:
[[Category: Unreleased Structures]]
[[Category: Huang, C.-Y]]