5d8k - Revision history

OCA at 12:23, 6 March 2024

OCA — Wed, 06 Mar 2024 12:23:15 GMT

←Older revision		Revision as of 12:23, 6 March 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5d8k]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5D8K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5D8K FirstGlance]. <br>		<table><tr><td colspan='2'>[[5d8k]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5D8K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5D8K FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5d8k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5d8k OCA], [https://pdbe.org/5d8k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5d8k RCSB], [https://www.ebi.ac.uk/pdbsum/5d8k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5d8k ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.728Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5d8k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5d8k OCA], [https://pdbe.org/5d8k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5d8k RCSB], [https://www.ebi.ac.uk/pdbsum/5d8k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5d8k ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/HSF2_HUMAN HSF2_HUMAN] DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked.		[https://www.uniprot.org/uniprot/HSF2_HUMAN HSF2_HUMAN] DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked.
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Heat-shock transcription factor (HSF) family members function in stress protection and in human diseases including proteopathies, neurodegeneration and cancer. The mechanisms that drive distinct post-translational modifications, cofactor recruitment and target-gene activation for specific HSF paralogs are unknown. We present crystal structures of the human HSF2 DNA-binding domain (DBD) bound to DNA, revealing an unprecedented view of HSFs that provides insights into their unique biology. The HSF2 DBD structures resolve a new C-terminal helix that directs wrapping of the coiled-coil domain around DNA, thereby exposing paralog-specific sequences of the DBD surface for differential post-translational modifications and cofactor interactions. We further demonstrate a direct interaction between HSF1 and HSF2 through their coiled-coil domains. Together, these features provide a new model for HSF structure as the basis for differential and combinatorial regulation, which influences the transcriptional response to cellular stress.
-
-	Structures of HSF2 reveal mechanisms for differential regulation of human heat-shock factors.,Jaeger AM, Pemble CW 4th, Sistonen L, Thiele DJ Nat Struct Mol Biol. 2016 Jan 4. doi: 10.1038/nsmb.3150. PMID:26727490<ref>PMID:26727490</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 5d8k" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Heat shock factor\|Heat shock factor]]		*[[Heat shock factor\|Heat shock factor]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 10:25, 21 June 2023

OCA — Wed, 21 Jun 2023 10:25:46 GMT

←Older revision		Revision as of 10:25, 21 June 2023
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	<StructureSection load='5d8k' size='340' side='right'caption='[[5d8k]], [[Resolution\|resolution]] 1.73Å' scene=''>		<StructureSection load='5d8k' size='340' side='right'caption='[[5d8k]], [[Resolution\|resolution]] 1.73Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5d8k]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5D8K OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5D8K FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5d8k]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5D8K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5D8K FirstGlance]. <br>
-	</td></tr>~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5d8l\|5d8l]]</td></tr>~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5d8k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5d8k OCA], [https://pdbe.org/5d8k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5d8k RCSB], [https://www.ebi.ac.uk/pdbsum/5d8k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5d8k ProSAT]</span></td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">HSF2, HSTF2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>~~	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5d8k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5d8k OCA], [~~http~~://pdbe.org/5d8k PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5d8k RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5d8k PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5d8k ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/HSF2_HUMAN HSF2_HUMAN]] DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked.	+	[https://www.uniprot.org/uniprot/HSF2_HUMAN HSF2_HUMAN] DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 26:		Line 24:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Jaeger, A M~~]]	+	[[Category: Synthetic construct]]
-	[[Category: ~~Pemble, C W~~]]	+	[[Category: Jaeger AM]]
-	[[Category: ~~Thiele, D J~~]]	+	[[Category: Pemble CW]]
-	[[Category: ~~Dna]]~~	+	[[Category: Thiele DJ]]
-	~~[[Category: Hsf]]~~	+
-	~~[[Category: Transcription factor]]~~	+
-	~~[[Category: Transcription-dna complex~~]]	+

OCA at 10:43, 1 April 2020

OCA — Wed, 01 Apr 2020 10:43:01 GMT

←Older revision		Revision as of 10:43, 1 April 2020
Line 1:		Line 1:
		+
	==Human HSF2 DNA-Binding Domain bound to 2-site HSE DNA at 1.73 Angstroms Resolution==		==Human HSF2 DNA-Binding Domain bound to 2-site HSE DNA at 1.73 Angstroms Resolution==
-	<StructureSection load='5d8k' size='340' side='right' caption='[[5d8k]], [[Resolution\|resolution]] 1.73Å' scene=''>	+	<StructureSection load='5d8k' size='340' side='right'caption='[[5d8k]], [[Resolution\|resolution]] 1.73Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5d8k]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5D8K OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5D8K FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5d8k]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5D8K OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5D8K FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5d8l\|5d8l]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5d8l\|5d8l]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5d8k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5d8k OCA], [http://pdbe.org/5d8k PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5d8k RCSB], [http://www.ebi.ac.uk/pdbsum/5d8k PDBsum]</span></td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">HSF2, HSTF2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5d8k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5d8k OCA], [http://pdbe.org/5d8k PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5d8k RCSB], [http://www.ebi.ac.uk/pdbsum/5d8k PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5d8k ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 17:		Line 19:
	</div>		</div>
	<div class="pdbe-citations 5d8k" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5d8k" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Heat shock factor\|Heat shock factor]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Jaeger, A M]]		[[Category: Jaeger, A M]]
	[[Category: Pemble, C W]]		[[Category: Pemble, C W]]

OCA at 07:51, 3 February 2016

OCA — Wed, 03 Feb 2016 07:51:23 GMT

←Older revision		Revision as of 07:51, 3 February 2016
Line 8:		Line 8:
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/HSF2_HUMAN HSF2_HUMAN]] DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked.		[[http://www.uniprot.org/uniprot/HSF2_HUMAN HSF2_HUMAN]] DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Heat-shock transcription factor (HSF) family members function in stress protection and in human diseases including proteopathies, neurodegeneration and cancer. The mechanisms that drive distinct post-translational modifications, cofactor recruitment and target-gene activation for specific HSF paralogs are unknown. We present crystal structures of the human HSF2 DNA-binding domain (DBD) bound to DNA, revealing an unprecedented view of HSFs that provides insights into their unique biology. The HSF2 DBD structures resolve a new C-terminal helix that directs wrapping of the coiled-coil domain around DNA, thereby exposing paralog-specific sequences of the DBD surface for differential post-translational modifications and cofactor interactions. We further demonstrate a direct interaction between HSF1 and HSF2 through their coiled-coil domains. Together, these features provide a new model for HSF structure as the basis for differential and combinatorial regulation, which influences the transcriptional response to cellular stress.
		+
		+	Structures of HSF2 reveal mechanisms for differential regulation of human heat-shock factors.,Jaeger AM, Pemble CW 4th, Sistonen L, Thiele DJ Nat Struct Mol Biol. 2016 Jan 4. doi: 10.1038/nsmb.3150. PMID:26727490<ref>PMID:26727490</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5d8k" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 11:31, 7 January 2016

OCA — Thu, 07 Jan 2016 11:31:43 GMT

←Older revision		Revision as of 11:31, 7 January 2016
Line 1:		Line 1:
-	'''~~Unreleased structure~~'''	+	==Human HSF2 DNA-Binding Domain bound to 2-site HSE DNA at 1.73 Angstroms Resolution==
-		+	<StructureSection load='5d8k' size='340' side='right' caption='[[5d8k]], [[Resolution\|resolution]] 1.73Å' scene=''>
-	~~The entry~~ 5d8k is ~~ON HOLD until Jan 10 2017~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5d8k]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5D8K OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5D8K FirstGlance]. <br>
-	~~Authors~~: ~~Jaeger~~, A.M., ~~Pemble~~, C.W., ~~Thiele, D~~.J.	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5d8l\|5d8l]]</td></tr>
-		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5d8k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5d8k OCA], [http://pdbe.org/5d8k PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5d8k RCSB], [http://www.ebi.ac.uk/pdbsum/5d8k PDBsum]</span></td></tr>
-	~~Description~~: ~~Human HSF2~~ DNA-~~Binding Domain bound~~ to ~~2-site~~ HSE ~~DNA at 1~~.~~73 Angstroms Resolution~~	+	</table>
-	[[Category: ~~Unreleased Structures~~]]	+	== Function ==
-	[[Category: Thiele, D.J]]	+	[[http://www.uniprot.org/uniprot/HSF2_HUMAN HSF2_HUMAN]] DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked.
-	[[Category: ~~Pemble, C.W~~]]	+	__TOC__
-	[[Category: ~~Jaeger, A.M~~]]	+	</StructureSection>
		+	[[Category: Jaeger, A M]]
		+	[[Category: Pemble, C W]]
		+	[[Category: Thiele, D J]]
		+	[[Category: Dna]]
		+	[[Category: Hsf]]
		+	[[Category: Transcription factor]]
		+	[[Category: Transcription-dna complex]]

OCA at 11:00, 16 December 2015

OCA — Wed, 16 Dec 2015 11:00:06 GMT

←Older revision		Revision as of 11:00, 16 December 2015
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5d8k is ON HOLD until ~~Paper Publication~~	+	The entry 5d8k is ON HOLD until Jan 10 2017

	Authors: Jaeger, A.M., Pemble, C.W., Thiele, D.J.		Authors: Jaeger, A.M., Pemble, C.W., Thiele, D.J.
Line 7:		Line 7:
	Description: Human HSF2 DNA-Binding Domain bound to 2-site HSE DNA at 1.73 Angstroms Resolution		Description: Human HSF2 DNA-Binding Domain bound to 2-site HSE DNA at 1.73 Angstroms Resolution
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Jaeger, A.M]]
	[[Category: Thiele, D.J]]		[[Category: Thiele, D.J]]
	[[Category: Pemble, C.W]]		[[Category: Pemble, C.W]]
		+	[[Category: Jaeger, A.M]]

OCA at 09:55, 9 September 2015

OCA — Wed, 09 Sep 2015 09:55:46 GMT

←Older revision		Revision as of 09:55, 9 September 2015
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5d8k is ON HOLD	+	The entry 5d8k is ON HOLD until Paper Publication

-	Authors: Jaeger, A.M., Pemble, C.W.,, Thiele, D.J.	+	Authors: Jaeger, A.M., Pemble, C.W., Thiele, D.J.

	Description: Human HSF2 DNA-Binding Domain bound to 2-site HSE DNA at 1.73 Angstroms Resolution		Description: Human HSF2 DNA-Binding Domain bound to 2-site HSE DNA at 1.73 Angstroms Resolution
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: Jaeger, A.M]]		[[Category: Jaeger, A.M]]
-	[[Category: , Thiele, D.J]]	+	[[Category: Thiele, D.J]]
	[[Category: Pemble, C.W]]		[[Category: Pemble, C.W]]

OCA: Protected "5d8k" [edit=sysop:move=sysop]

OCA — Wed, 26 Aug 2015 12:33:37 GMT

Protected "5d8k" [edit=sysop:move=sysop]

←Older revision

Revision as of 12:33, 26 August 2015

OCA: New page: '''Unreleased structure''' The entry 5d8k is ON HOLD Authors: Jaeger, A.M., Pemble, C.W.,, Thiele, D.J. Description: Human HSF2 DNA-Binding Domain bound to 2-site HSE DNA at 1.73 Angst...

OCA — Wed, 26 Aug 2015 12:33:35 GMT

New page: '''Unreleased structure''' The entry 5d8k is ON HOLD Authors: Jaeger, A.M., Pemble, C.W.,, Thiele, D.J. Description: Human HSF2 DNA-Binding Domain bound to 2-site HSE DNA at 1.73 Angst...

New page

'''Unreleased structure'''

The entry 5d8k is ON HOLD

Authors: Jaeger, A.M., Pemble, C.W.,, Thiele, D.J.

Description: Human HSF2 DNA-Binding Domain bound to 2-site HSE DNA at 1.73 Angstroms Resolution
[[Category: Unreleased Structures]]
[[Category: Jaeger, A.M]]
[[Category: , Thiele, D.J]]
[[Category: Pemble, C.W]]