5d8w - Revision history

OCA at 10:26, 21 June 2023

OCA — Wed, 21 Jun 2023 10:26:17 GMT

←Older revision		Revision as of 10:26, 21 June 2023
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	<StructureSection load='5d8w' size='340' side='right'caption='[[5d8w]], [[Resolution\|resolution]] 2.86Å' scene=''>		<StructureSection load='5d8w' size='340' side='right'caption='[[5d8w]], [[Resolution\|resolution]] 2.86Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5d8w]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Bjfc:cui-9207 Bjfc:cui-9207~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5D8W OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5D8W FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5d8w]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Ganoderma_lucidum Ganoderma lucidum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5D8W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5D8W FirstGlance]. <br>
-	</td></tr>~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5d8z\|5d8z]]</td></tr>~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5d8w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5d8w OCA], [https://pdbe.org/5d8w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5d8w RCSB], [https://www.ebi.ac.uk/pdbsum/5d8w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5d8w ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5d8w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5d8w OCA], [~~http~~://pdbe.org/5d8w PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5d8w RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5d8w PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5d8w ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A0A1A9TAJ1_GANLU A0A1A9TAJ1_GANLU]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 20:		Line 21:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Bjfc:cui-9207]]
-	[[Category: Large Structures]]
-	[[Category: Chen, C C]]
-	[[Category: Guo, R]]
-	[[Category: Ko, T P]]
-	[[Category: Li, Q]]
-	[[Category: Liu, G]]
-	[[Category: Liu, W]]
-	[[Category: Shang, N]]
-	[[Category: Endoglucanase]]
	[[Category: Ganoderma lucidum]]		[[Category: Ganoderma lucidum]]
-	[[Category: ~~Gh5 family~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Hydrolase~~]]	+	[[Category: Chen CC]]
		+	[[Category: Guo R]]
		+	[[Category: Ko TP]]
		+	[[Category: Li Q]]
		+	[[Category: Liu G]]
		+	[[Category: Liu W]]
		+	[[Category: Shang N]]

OCA at 10:43, 1 April 2020

OCA — Wed, 01 Apr 2020 10:43:11 GMT

←Older revision		Revision as of 10:43, 1 April 2020
Line 1:		Line 1:

	==Structrue of a lucidum protein==		==Structrue of a lucidum protein==
-	<StructureSection load='5d8w' size='340' side='right' caption='[[5d8w]], [[Resolution\|resolution]] 2.86Å' scene=''>	+	<StructureSection load='5d8w' size='340' side='right'caption='[[5d8w]], [[Resolution\|resolution]] 2.86Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5d8w]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bjfc:cui-9207 Bjfc:cui-9207]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5D8W OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5D8W FirstGlance]. <br>		<table><tr><td colspan='2'>[[5d8w]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bjfc:cui-9207 Bjfc:cui-9207]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5D8W OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5D8W FirstGlance]. <br>
Line 21:		Line 21:
	</StructureSection>		</StructureSection>
	[[Category: Bjfc:cui-9207]]		[[Category: Bjfc:cui-9207]]
		+	[[Category: Large Structures]]
	[[Category: Chen, C C]]		[[Category: Chen, C C]]
	[[Category: Guo, R]]		[[Category: Guo, R]]

OCA at 07:35, 23 May 2018

OCA — Wed, 23 May 2018 07:35:09 GMT

←Older revision		Revision as of 07:35, 23 May 2018
Line 3:		Line 3:
	<StructureSection load='5d8w' size='340' side='right' caption='[[5d8w]], [[Resolution\|resolution]] 2.86Å' scene=''>		<StructureSection load='5d8w' size='340' side='right' caption='[[5d8w]], [[Resolution\|resolution]] 2.86Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5d8w]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5D8W OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5D8W FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5d8w]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bjfc:cui-9207 Bjfc:cui-9207]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5D8W OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5D8W FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5d8z\|5d8z]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5d8z\|5d8z]]</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5d8w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5d8w OCA], [http://pdbe.org/5d8w PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5d8w RCSB], [http://www.ebi.ac.uk/pdbsum/5d8w PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5d8w ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5d8w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5d8w OCA], [http://pdbe.org/5d8w PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5d8w RCSB], [http://www.ebi.ac.uk/pdbsum/5d8w PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5d8w ProSAT]</span></td></tr>
Line 20:		Line 20:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Bjfc:cui-9207]]
	[[Category: Chen, C C]]		[[Category: Chen, C C]]
	[[Category: Guo, R]]		[[Category: Guo, R]]

OCA at 10:20, 13 July 2016

OCA — Wed, 13 Jul 2016 10:20:55 GMT

←Older revision		Revision as of 10:20, 13 July 2016
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5d8w is ~~ON HOLD until Paper~~ Publication	+	==Structrue of a lucidum protein==
		+	<StructureSection load='5d8w' size='340' side='right' caption='[[5d8w]], [[Resolution\|resolution]] 2.86Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5d8w]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5D8W OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5D8W FirstGlance]. <br>
		+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5d8z\|5d8z]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5d8w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5d8w OCA], [http://pdbe.org/5d8w PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5d8w RCSB], [http://www.ebi.ac.uk/pdbsum/5d8w PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5d8w ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Ganoderma lucidum is a saprotrophic white-rot fungus which contains a rich set of cellulolytic enzymes. Here, we screened an array of potential 1,4-beta-endoglucanases from G. lucidum based on the gene annotation library and found that one candidate gene, GlCel5A, exhibits CMC-hydrolyzing activity. The recombinant GlCel5A protein expressed in Pichia pastoris is able to hydrolyze CMC and beta-glucan but not xylan and mannan. The enzyme exhibits optimal activity at 60 degrees C and pH 3-4, and retained 50% activity at 80 and 90 degrees C for at least 15 and 10min. The crystal structure of GlCel5A and its complex with cellobiose, solved at 2.7 and 2.86A resolution, shows a classical (beta/alpha)8 TIM-barrel fold as seen in other members of glycoside hydrolase family 5. The complex structure contains a cellobiose molecule in the +1 and +2 subsites, and reveals the interactions with the positive sites of the enzyme. Collectively, the present work provides the first comprehensive characterization of an endoglucanase from G. lucidum that possesses properties for industrial applications, and strongly encourages further studying in the cellulolytic enzyme system of G. lucidum.

-	~~Authors: Guo~~, R., Li, Q., Shang, N., Liu, G., Ko, ~~T.P.~~, Chen, C.C., ~~Liu, W~~.	+	Functional and structural analyses of a 1,4-beta-endoglucanase from Ganoderma lucidum.,Liu G, Li Q, Shang N, Huang JW, Ko TP, Liu W, Zheng Y, Han X, Chen Y, Chen CC, Jin J, Guo RT Enzyme Microb Technol. 2016 May;86:67-74. doi: 10.1016/j.enzmictec.2016.01.013., Epub 2016 Feb 1. PMID:26992795<ref>PMID:26992795</ref>

-	~~Description: Structrue~~ of ~~a lucidum protein~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category~~: ~~Unreleased Structures]]~~	+	</div>
-	~~[[Category: Shang, N]]~~	+	<div class="pdbe-citations 5d8w" style="background-color:#fffaf0;"></div>
-	[[Category: ~~Liu~~, W]]	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Chen, C C]]
	[[Category: Guo, R]]		[[Category: Guo, R]]
-	~~[[Category: Chen, C.C]]~~	+	[[Category: Ko, T P]]
-	[[Category: Ko, T.P]]	+
	[[Category: Li, Q]]		[[Category: Li, Q]]
	[[Category: Liu, G]]		[[Category: Liu, G]]
		+	[[Category: Liu, W]]
		+	[[Category: Shang, N]]
		+	[[Category: Endoglucanase]]
		+	[[Category: Ganoderma lucidum]]
		+	[[Category: Gh5 family]]
		+	[[Category: Hydrolase]]

OCA at 11:00, 16 December 2015

OCA — Wed, 16 Dec 2015 11:00:28 GMT

←Older revision		Revision as of 11:00, 16 December 2015
Line 7:		Line 7:
	Description: Structrue of a lucidum protein		Description: Structrue of a lucidum protein
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Ko, T.P]]
	[[Category: Shang, N]]		[[Category: Shang, N]]
	[[Category: Liu, W]]		[[Category: Liu, W]]
	[[Category: Guo, R]]		[[Category: Guo, R]]
	[[Category: Chen, C.C]]		[[Category: Chen, C.C]]
		+	[[Category: Ko, T.P]]
	[[Category: Li, Q]]		[[Category: Li, Q]]
	[[Category: Liu, G]]		[[Category: Liu, G]]

OCA at 13:35, 2 December 2015

OCA — Wed, 02 Dec 2015 13:35:53 GMT

←Older revision		Revision as of 13:35, 2 December 2015
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5d8w is ON HOLD	+	The entry 5d8w is ON HOLD until Paper Publication

	Authors: Guo, R., Li, Q., Shang, N., Liu, G., Ko, T.P., Chen, C.C., Liu, W.		Authors: Guo, R., Li, Q., Shang, N., Liu, G., Ko, T.P., Chen, C.C., Liu, W.

OCA: Protected "5d8w" [edit=sysop:move=sysop]

OCA — Wed, 26 Aug 2015 12:34:33 GMT

Protected "5d8w" [edit=sysop:move=sysop]

←Older revision

Revision as of 12:34, 26 August 2015

OCA: New page: '''Unreleased structure''' The entry 5d8w is ON HOLD Authors: Guo, R., Li, Q., Shang, N., Liu, G., Ko, T.P., Chen, C.C., Liu, W. Description: Structrue of a lucidum protein [[Category:...

OCA — Wed, 26 Aug 2015 12:34:32 GMT

New page: '''Unreleased structure''' The entry 5d8w is ON HOLD Authors: Guo, R., Li, Q., Shang, N., Liu, G., Ko, T.P., Chen, C.C., Liu, W. Description: Structrue of a lucidum protein [[Category:...

New page

'''Unreleased structure'''

The entry 5d8w is ON HOLD

Authors: Guo, R., Li, Q., Shang, N., Liu, G., Ko, T.P., Chen, C.C., Liu, W.

Description: Structrue of a lucidum protein
[[Category: Unreleased Structures]]
[[Category: Ko, T.P]]
[[Category: Shang, N]]
[[Category: Liu, W]]
[[Category: Guo, R]]
[[Category: Chen, C.C]]
[[Category: Li, Q]]
[[Category: Liu, G]]