5daj - Revision history

OCA at 20:37, 12 April 2023

OCA — Wed, 12 Apr 2023 20:37:00 GMT

←Older revision		Revision as of 20:37, 12 April 2023
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	==Crystal structure of NalD, the secondary repressor of MexAB-OprM multidrug efflux pump in Pseudomonas aeruginosa==		==Crystal structure of NalD, the secondary repressor of MexAB-OprM multidrug efflux pump in Pseudomonas aeruginosa==
-	<StructureSection load='5daj' size='340' side='right' caption='[[5daj]], [[Resolution\|resolution]] 2.65Å' scene=''>	+	<StructureSection load='5daj' size='340' side='right'caption='[[5daj]], [[Resolution\|resolution]] 2.65Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5daj]] is a 8 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Pseae Pseae~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5DAJ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5DAJ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5daj]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5DAJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5DAJ FirstGlance]. <br>
-	</td></tr><tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">nalD ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=208964 PSEAE])~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5daj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5daj OCA], [https://pdbe.org/5daj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5daj RCSB], [https://www.ebi.ac.uk/pdbsum/5daj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5daj ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5daj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5daj OCA], [~~http~~://pdbe.org/5daj PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5daj RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5daj PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5daj ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q9HY46_PSEAE Q9HY46_PSEAE]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 21:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Pseae~~]]	+	[[Category: Large Structures]]
-	[[Category: Chen, H]]	+	[[Category: Pseudomonas aeruginosa PAO1]]
-	[[Category: Chen~~, W Z~~]]	+	[[Category: Chen H]]
-	[[Category: Gan~~, J H~~]]	+	[[Category: Chen WZ]]
-	[[Category: Hu~~, Q Y~~]]	+	[[Category: Gan JH]]
-	[[Category: Huang~~, S Q~~]]	+	[[Category: Hu QY]]
-	[[Category: Liu~~, X C~~]]	+	[[Category: Huang SQ]]
-	[[Category: Wang, D]]	+	[[Category: Liu XC]]
-	~~[[Category: Repressor]]~~	+	[[Category: Wang D]]
-	~~[[Category: Tetr family]]~~	+
-	~~[[Category: Transcription regulator]]~~	+
-	~~[[Category: Transcriptional regulator~~]]	+

OCA at 12:31, 16 November 2017

OCA — Thu, 16 Nov 2017 12:31:03 GMT

←Older revision		Revision as of 12:31, 16 November 2017
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	<StructureSection load='5daj' size='340' side='right' caption='[[5daj]], [[Resolution\|resolution]] 2.65Å' scene=''>		<StructureSection load='5daj' size='340' side='right' caption='[[5daj]], [[Resolution\|resolution]] 2.65Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5daj]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5DAJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5DAJ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5daj]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseae Pseae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5DAJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5DAJ FirstGlance]. <br>
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5daj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5daj OCA], [http://pdbe.org/5daj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5daj RCSB], [http://www.ebi.ac.uk/pdbsum/5daj PDBsum]</span></td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">nalD ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=208964 PSEAE])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5daj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5daj OCA], [http://pdbe.org/5daj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5daj RCSB], [http://www.ebi.ac.uk/pdbsum/5daj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5daj ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Pseae]]
	[[Category: Chen, H]]		[[Category: Chen, H]]
	[[Category: Chen, W Z]]		[[Category: Chen, W Z]]

OCA at 18:42, 12 May 2016

OCA — Thu, 12 May 2016 18:42:14 GMT

←Older revision		Revision as of 18:42, 12 May 2016
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5daj is ~~ON HOLD until Paper~~ Publication	+	==Crystal structure of NalD, the secondary repressor of MexAB-OprM multidrug efflux pump in Pseudomonas aeruginosa==
		+	<StructureSection load='5daj' size='340' side='right' caption='[[5daj]], [[Resolution\|resolution]] 2.65Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5daj]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5DAJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5DAJ FirstGlance]. <br>
		+	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5daj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5daj OCA], [http://pdbe.org/5daj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5daj RCSB], [http://www.ebi.ac.uk/pdbsum/5daj PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	NalD was reported to be the secondary repressor of the MexAB-OprM multidrug efflux pump, the major system contributing to intrinsic multidrug resistance in Pseudomonas aeruginosa. Here, we show that novobiocin binds directly to NalD, which leads NalD to dissociate from the DNA promoter, and thus de-represses the expression of the MexAB-OprM pump. In addition, we have solved the crystal structure of NalD at a resolution of 2.90 A. The structural alignment of NalD to its homologue TtgR reveals that the residues N129 and H167 in NalD are involved in its novobiocin-binding ability. We have confirmed the function of these two amino acids by EMSA and plate assay. The results presented here highlight the importance and diversity of regulatory mechanism in bacterial antibiotic resistance, and provide further insight for novel antimicrobial development. This article is protected by copyright. All rights reserved.

-	~~Authors: Chen~~, W~~.Z.~~, Wang, D., ~~Huang~~, ~~S.Q., Hu, Q.Y.~~, Liu, X~~.C.~~, ~~Gan~~, J~~.H.~~, ~~Chen~~, H.	+	Novobiocin binding to NalD induces the expression of the MexAB-OprM pump in Pseudomonas aeruginosa.,Chen W, Wang D, Zhou W, Sang H, Liu X, Ge Z, Zhang J, Lan L, Yang CG, Chen H Mol Microbiol. 2016 Feb 4. doi: 10.1111/mmi.13346. PMID:26844397<ref>PMID:26844397</ref>

-	~~Description: Crystal structure of NalD~~, the ~~secondary repressor~~ of ~~MexAB-OprM multidrug efflux pump in Pseudomonas aeruginosa~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category: Unreleased Structures]]~~	+	</div>
-	~~[[Category~~: ~~Hu, Q.Y]]~~	+	<div class="pdbe-citations 5daj" style="background-color:#fffaf0;"></div>
-	~~[[Category: Wang, D]]~~	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Chen, H]]		[[Category: Chen, H]]
-	[[Category: ~~Liu~~, ~~X.C~~]]	+	[[Category: Chen, W Z]]
-	[[Category: Gan, J.H]]	+	[[Category: Gan, J H]]
-	[[Category: ~~Chen~~, ~~W.Z~~]]	+	[[Category: Hu, Q Y]]
-	[[Category: Huang, S.Q]]	+	[[Category: Huang, S Q]]
		+	[[Category: Liu, X C]]
		+	[[Category: Wang, D]]
		+	[[Category: Repressor]]
		+	[[Category: Tetr family]]
		+	[[Category: Transcription regulator]]
		+	[[Category: Transcriptional regulator]]

OCA at 08:44, 7 January 2016

OCA — Thu, 07 Jan 2016 08:44:35 GMT

←Older revision		Revision as of 08:44, 7 January 2016
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5daj is ON HOLD	+	The entry 5daj is ON HOLD until Paper Publication

	Authors: Chen, W.Z., Wang, D., Huang, S.Q., Hu, Q.Y., Liu, X.C., Gan, J.H., Chen, H.		Authors: Chen, W.Z., Wang, D., Huang, S.Q., Hu, Q.Y., Liu, X.C., Gan, J.H., Chen, H.

OCA at 11:02, 16 December 2015

OCA — Wed, 16 Dec 2015 11:02:08 GMT

←Older revision		Revision as of 11:02, 16 December 2015
Line 9:		Line 9:
	[[Category: Hu, Q.Y]]		[[Category: Hu, Q.Y]]
	[[Category: Wang, D]]		[[Category: Wang, D]]
-	[[Category: Liu, X.C]]
	[[Category: Chen, H]]		[[Category: Chen, H]]
		+	[[Category: Liu, X.C]]
	[[Category: Gan, J.H]]		[[Category: Gan, J.H]]
	[[Category: Chen, W.Z]]		[[Category: Chen, W.Z]]
	[[Category: Huang, S.Q]]		[[Category: Huang, S.Q]]

OCA: Protected "5daj" [edit=sysop:move=sysop]

OCA — Wed, 26 Aug 2015 12:39:49 GMT

Protected "5daj" [edit=sysop:move=sysop]

←Older revision

Revision as of 12:39, 26 August 2015

OCA: New page: '''Unreleased structure''' The entry 5daj is ON HOLD Authors: Chen, W.Z., Wang, D., Huang, S.Q., Hu, Q.Y., Liu, X.C., Gan, J.H., Chen, H. Description: Crystal structure of NalD, the se...

OCA — Wed, 26 Aug 2015 12:39:48 GMT

New page: '''Unreleased structure''' The entry 5daj is ON HOLD Authors: Chen, W.Z., Wang, D., Huang, S.Q., Hu, Q.Y., Liu, X.C., Gan, J.H., Chen, H. Description: Crystal structure of NalD, the se...

New page

'''Unreleased structure'''

The entry 5daj is ON HOLD

Authors: Chen, W.Z., Wang, D., Huang, S.Q., Hu, Q.Y., Liu, X.C., Gan, J.H., Chen, H.

Description: Crystal structure of NalD, the secondary repressor of MexAB-OprM multidrug efflux pump in Pseudomonas aeruginosa
[[Category: Unreleased Structures]]
[[Category: Hu, Q.Y]]
[[Category: Wang, D]]
[[Category: Liu, X.C]]
[[Category: Chen, H]]
[[Category: Gan, J.H]]
[[Category: Chen, W.Z]]
[[Category: Huang, S.Q]]