5dd0 - Revision history

OCA at 08:45, 27 September 2023

OCA — Wed, 27 Sep 2023 08:45:33 GMT

←Older revision		Revision as of 08:45, 27 September 2023
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5dd0]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Human_immunodeficiency_virus_1 Human immunodeficiency virus 1] and [https://en.wikipedia.org/wiki/Macaca_mulatta Macaca mulatta]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5DD0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5DD0 FirstGlance]. <br>		<table><tr><td colspan='2'>[[5dd0]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Human_immunodeficiency_virus_1 Human immunodeficiency virus 1] and [https://en.wikipedia.org/wiki/Macaca_mulatta Macaca mulatta]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5DD0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5DD0 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.488Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5dd0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5dd0 OCA], [https://pdbe.org/5dd0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5dd0 RCSB], [https://www.ebi.ac.uk/pdbsum/5dd0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5dd0 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5dd0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5dd0 OCA], [https://pdbe.org/5dd0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5dd0 RCSB], [https://www.ebi.ac.uk/pdbsum/5dd0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5dd0 ProSAT]</span></td></tr>
	</table>		</table>

OCA at 10:30, 21 June 2023

OCA — Wed, 21 Jun 2023 10:30:44 GMT

←Older revision		Revision as of 10:30, 21 June 2023
Line 3:		Line 3:
	<StructureSection load='5dd0' size='340' side='right'caption='[[5dd0]], [[Resolution\|resolution]] 2.49Å' scene=''>		<StructureSection load='5dd0' size='340' side='right'caption='[[5dd0]], [[Resolution\|resolution]] 2.49Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5dd0]] is a 3 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~9hiv1 9hiv1~~] and [~~http~~://en.wikipedia.org/wiki/~~Macmu Macmu~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5DD0 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5DD0 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5dd0]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Human_immunodeficiency_virus_1 Human immunodeficiency virus 1] and [https://en.wikipedia.org/wiki/Macaca_mulatta Macaca mulatta]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5DD0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5DD0 FirstGlance]. <br>
-	</td></tr><tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5dd1\|5dd1]], [[5dd3\|5dd3]], [[5dd5\|5dd5]], [[5dd6\|5dd6]]~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5dd0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5dd0 OCA], [https://pdbe.org/5dd0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5dd0 RCSB], [https://www.ebi.ac.uk/pdbsum/5dd0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5dd0 ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5dd0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5dd0 OCA], [~~http~~://pdbe.org/5dd0 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5dd0 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5dd0 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5dd0 ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/ENV_HV1H2 ENV_HV1H2] The surface protein gp120 (SU) attaches the virus to the host lymphoid cell by binding to the primary receptor CD4. This interaction induces a structural rearrangement creating a high affinity binding site for a chemokine coreceptor like CXCR4 and/or CCR5. This peculiar 2 stage receptor-interaction strategy allows gp120 to maintain the highly conserved coreceptor-binding site in a cryptic conformation, protected from neutralizing antibodies. Since CD4 also displays a binding site for the disulfide-isomerase P4HB/PDI, a P4HB/PDI-CD4-CXCR4-gp120 complex may form. In that complex, P4HB/PDI could reach and reduce gp120 disulfide bonds, causing major conformational changes in gp120. TXN, another PDI family member could also be involved in disulfide rearrangements in Env during fusion. These changes are transmitted to the transmembrane protein gp41 and are thought to activate its fusogenic potential by unmasking its fusion peptide.<ref>PMID:19410541</ref> Surface protein gp120 (SU) may target the virus to gut-associated lymphoid tissue (GALT) by binding host ITGA4/ITGB7 (alpha-4/beta-7 integrins), a complex that mediates T-cell migration to the GALT. Interaction between gp120 and ITGA4/ITGB7 would allow the virus to enter GALT early in the infection, infecting and killing most of GALT's resting CD4+ T-cells. This T-cell depletion is believed to be the major insult to the host immune system leading to AIDS (By similarity).<ref>PMID:19410541</ref> The surface protein gp120 is a ligand for CD209/DC-SIGN and CLEC4M/DC-SIGNR, which are respectively found on dendritic cells (DCs), and on endothelial cells of liver sinusoids and lymph node sinuses. These interactions allow capture of viral particles at mucosal surfaces by these cells and subsequent transmission to permissive cells. DCs are professional antigen presenting cells, critical for host immunity by inducing specific immune responses against a broad variety of pathogens. They act as sentinels in various tissues where they take up antigen, process it, and present it to T-cells following migration to lymphoid organs. HIV subverts the migration properties of dendritic cells to gain access to CD4+ T-cells in lymph nodes. Virus transmission to permissive T-cells occurs either in trans (without DCs infection, through viral capture and transmission), or in cis (following DCs productive infection, through the usual CD4-gp120 interaction), thereby inducing a robust infection. In trans infection, bound virions remain infectious over days and it is proposed that they are not degraded, but protected in non-lysosomal acidic organelles within the DCs close to the cell membrane thus contributing to the viral infectious potential during DCs' migration from the periphery to the lymphoid tissues. On arrival at lymphoid tissues, intact virions recycle back to DCs' cell surface allowing virus transmission to CD4+ T-cells. Virion capture also seems to lead to MHC-II-restricted viral antigen presentation, and probably to the activation of HIV-specific CD4+ cells.<ref>PMID:19410541</ref> The transmembrane protein gp41 (TM) acts as a class I viral fusion protein. Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During fusion of viral and target intracellular membranes, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes. Complete fusion occurs in host cell endosomes and is dynamin-dependent, however some lipid transfer might occur at the plasma membrane. The virus undergoes clathrin-dependent internalization long before endosomal fusion, thus minimizing the surface exposure of conserved viral epitopes during fusion and reducing the efficacy of inhibitors targeting these epitopes. Membranes fusion leads to delivery of the nucleocapsid into the cytoplasm.<ref>PMID:19410541</ref> The envelope glyprotein gp160 precursor down-modulates cell surface CD4 antigen by interacting with it in the endoplasmic reticulum and blocking its transport to the cell surface (By similarity).<ref>PMID:19410541</ref> The gp120-gp41 heterodimer seems to contribute to T-cell depletion during HIV-1 infection. The envelope glycoproteins expressed on the surface of infected cells induce apoptosis through an interaction with uninfected cells expressing the receptor (CD4) and the coreceptors CXCR4 or CCR5. This type of bystander killing may be obtained by at least three distinct mechanisms. First, the interaction between the 2 cells can induce cellular fusion followed by nuclear fusion within the syncytium. Syncytia are condemned to die from apoptosis. Second, the 2 interacting cells may not fuse entirely and simply exchange plasma membrane lipids, after a sort of hemifusion process, followed by rapid death. Third, it is possible that virus-infected cells, on the point of undergoing apoptosis, fuse with CD4-expressing cells, in which case apoptosis is rapidly transmitted from one cell to the other and thus occurs in a sort of contagious fashion.<ref>PMID:19410541</ref> The gp120-gp41 heterodimer allows rapid transcytosis of the virus through CD4 negative cells such as simple epithelial monolayers of the intestinal, rectal and endocervical epithelial barriers. Both gp120 and gp41 specifically recognize glycosphingolipids galactosyl-ceramide (GalCer) or 3' sulfo-galactosyl-ceramide (GalS) present in the lipid rafts structures of epithelial cells. Binding to these alternative receptors allows the rapid transcytosis of the virus through the epithelial cells. This transcytotic vesicle-mediated transport of virions from the apical side to the basolateral side of the epithelial cells does not involve infection of the cells themselves.<ref>PMID:19410541</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 20:		Line 21:
	==See Also==		==See Also==
	*[[Antibody 3D structures\|Antibody 3D structures]]		*[[Antibody 3D structures\|Antibody 3D structures]]
		+	*[[3D structures of non-human antibody\|3D structures of non-human antibody]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Human immunodeficiency virus 1]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Macmu~~]]	+	[[Category: Macaca mulatta]]
-	[[Category: Alam~~, S M~~]]	+	[[Category: Alam SM]]
-	[[Category: Bouton-Verville, H]]	+	[[Category: Bouton-Verville H]]
-	[[Category: Bradley, T]]	+	[[Category: Bradley T]]
-	[[Category: Foulger, A]]	+	[[Category: Foulger A]]
-	[[Category: Gao, F]]	+	[[Category: Gao F]]
-	[[Category: Haynes~~, B F~~]]	+	[[Category: Haynes BF]]
-	[[Category: Kelsoe, G]]	+	[[Category: Kelsoe G]]
-	[[Category: Keple~~, T B~~]]	+	[[Category: Keple TB]]
-	[[Category: Liao, H X]]	+	[[Category: Liao H-X]]
-	[[Category: Montefiori~~, D C~~]]	+	[[Category: Montefiori DC]]
-	[[Category: Moody~~, M A~~]]	+	[[Category: Moody MA]]
-	[[Category: Nicely~~, N I~~]]	+	[[Category: Nicely NI]]
-	[[Category: Parks, R]]	+	[[Category: Parks R]]
-	[[Category: Pemble, C]]	+	[[Category: Pemble C]]
-	[[Category: Santra, S]]	+	[[Category: Santra S]]
-	[[Category: Tomaras, G]]	+	[[Category: Tomaras G]]
-	[[Category: Verkoczy, L]]	+	[[Category: Verkoczy L]]
-	[[Category: Wiehe, K]]	+	[[Category: Wiehe K]]
-	[[Category: Zhang, R]]	+	[[Category: Zhang R]]
-	~~[[Category: Hiv hiv-1 gp41 mper antibody]]~~	+
-	~~[[Category: Immune system~~]]	+

OCA at 16:03, 11 December 2019

OCA — Wed, 11 Dec 2019 16:03:03 GMT

←Older revision		Revision as of 16:03, 11 December 2019
Line 1:		Line 1:

	==Crystal structures in an anti-HIV antibody lineage from immunization of Rhesus macaques==		==Crystal structures in an anti-HIV antibody lineage from immunization of Rhesus macaques==
-	<StructureSection load='5dd0' size='340' side='right' caption='[[5dd0]], [[Resolution\|resolution]] 2.49Å' scene=''>	+	<StructureSection load='5dd0' size='340' side='right'caption='[[5dd0]], [[Resolution\|resolution]] 2.49Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5dd0]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/9hiv1 9hiv1] and [http://en.wikipedia.org/wiki/Macmu Macmu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5DD0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5DD0 FirstGlance]. <br>		<table><tr><td colspan='2'>[[5dd0]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/9hiv1 9hiv1] and [http://en.wikipedia.org/wiki/Macmu Macmu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5DD0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5DD0 FirstGlance]. <br>
Line 19:		Line 19:

	==See Also==		==See Also==
-	*[[3D structures ~~of antibody~~\|3D structures ~~of antibody~~]]	+	*[[Antibody 3D structures\|Antibody 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Macmu]]		[[Category: Macmu]]
	[[Category: Alam, S M]]		[[Category: Alam, S M]]

OCA at 16:48, 15 November 2017

OCA — Wed, 15 Nov 2017 16:48:14 GMT

←Older revision		Revision as of 16:48, 15 November 2017
Line 3:		Line 3:
	<StructureSection load='5dd0' size='340' side='right' caption='[[5dd0]], [[Resolution\|resolution]] 2.49Å' scene=''>		<StructureSection load='5dd0' size='340' side='right' caption='[[5dd0]], [[Resolution\|resolution]] 2.49Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5dd0]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5DD0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5DD0 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5dd0]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/9hiv1 9hiv1] and [http://en.wikipedia.org/wiki/Macmu Macmu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5DD0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5DD0 FirstGlance]. <br>
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5dd1\|5dd1]], [[5dd3\|5dd3]], [[5dd5\|5dd5]], [[5dd6\|5dd6]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5dd1\|5dd1]], [[5dd3\|5dd3]], [[5dd5\|5dd5]], [[5dd6\|5dd6]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5dd0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5dd0 OCA], [http://pdbe.org/5dd0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5dd0 RCSB], [http://www.ebi.ac.uk/pdbsum/5dd0 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5dd0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5dd0 OCA], [http://pdbe.org/5dd0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5dd0 RCSB], [http://www.ebi.ac.uk/pdbsum/5dd0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5dd0 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 17:		Line 17:
	</div>		</div>
	<div class="pdbe-citations 5dd0" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5dd0" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[3D structures of antibody\|3D structures of antibody]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Macmu]]
	[[Category: Alam, S M]]		[[Category: Alam, S M]]
	[[Category: Bouton-Verville, H]]		[[Category: Bouton-Verville, H]]

OCA at 16:54, 15 May 2016

OCA — Sun, 15 May 2016 16:54:34 GMT

←Older revision		Revision as of 16:54, 15 May 2016
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5dd0 is ~~ON HOLD until Paper~~ Publication	+	==Crystal structures in an anti-HIV antibody lineage from immunization of Rhesus macaques==
		+	<StructureSection load='5dd0' size='340' side='right' caption='[[5dd0]], [[Resolution\|resolution]] 2.49Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5dd0]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5DD0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5DD0 FirstGlance]. <br>
		+	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5dd1\|5dd1]], [[5dd3\|5dd3]], [[5dd5\|5dd5]], [[5dd6\|5dd6]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5dd0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5dd0 OCA], [http://pdbe.org/5dd0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5dd0 RCSB], [http://www.ebi.ac.uk/pdbsum/5dd0 PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Development of an HIV vaccine is a global priority. A major roadblock to a vaccine is an inability to induce protective broadly neutralizing antibodies (bnAbs). HIV gp41 bnAbs have characteristics that predispose them to be controlled by tolerance. We used gp41 2F5 bnAb germline knock-in mice and macaques vaccinated with immunogens reactive with germline precursors to activate neutralizing antibodies. In germline knock-in mice, bnAb precursors were deleted, with remaining anergic B cells capable of being activated by germline-binding immunogens to make gp41-reactive immunoglobulin M (IgM). Immunized macaques made B cell clonal lineages targeted to the 2F5 bnAb epitope, but 2F5-like antibodies were either deleted or did not attain sufficient affinity for gp41-lipid complexes to achieve the neutralization potency of 2F5. Structural analysis of members of a vaccine-induced antibody lineage revealed that heavy chain complementarity-determining region 3 (HCDR3) hydrophobicity was important for neutralization. Thus, gp41 bnAbs are controlled by immune tolerance, requiring vaccination strategies to transiently circumvent tolerance controls.

-	~~Authors:~~ Zhang, R., Verkoczy, L., Wiehe, K., Alam, S~~.M.~~, Nicely~~, N.I.~~, Santra, S., Bradley, T., Pemble, C., Gao, F., Montefiori~~, D.C.~~, Bouton-Verville, H., Kelsoe, G., Parks, R., Foulger, A., ~~Tomaras~~, G., ~~Keple~~, ~~T.B.~~, ~~Moody~~, ~~M.A.~~, Liao, H.-X.~~, Haynes, B~~.F.	+	Initiation of immune tolerance-controlled HIV gp41 neutralizing B cell lineages.,Zhang R, Verkoczy L, Wiehe K, Munir Alam S, Nicely NI, Santra S, Bradley T, Pemble CW 4th, Zhang J, Gao F, Montefiori DC, Bouton-Verville H, Kelsoe G, Larimore K, Greenberg PD, Parks R, Foulger A, Peel JN, Luo K, Lu X, Trama AM, Vandergrift N, Tomaras GD, Kepler TB, Moody MA, Liao HX, Haynes BF Sci Transl Med. 2016 Apr 27;8(336):336ra62. doi: 10.1126/scitranslmed.aaf0618. PMID:27122615<ref>PMID:27122615</ref>

-	~~Description:~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category: Unreleased Structures]]~~	+	</div>
-	~~[[Category~~: ~~Tomaras, G]]~~	+	<div class="pdbe-citations 5dd0" style="background-color:#fffaf0;"></div>
-	~~[[Category: Moody, M.A]]~~	+	== References ==
-	~~[[Category: Haynes, B.F]]~~	+	<references/>
-	~~[[Category: Pemble, C]]~~	+	__TOC__
-	[[Category: ~~Foulger~~, A]]	+	</StructureSection>
		+	[[Category: Alam, S M]]
	[[Category: Bouton-Verville, H]]		[[Category: Bouton-Verville, H]]
-	[[Category: ~~Santra~~, S]]	+	[[Category: Bradley, T]]
-	[[Category: ~~Wiehe~~, ~~K]]~~	+	[[Category: Foulger, A]]
-	~~[[Category: Montefiori, D.C~~]]	+
	[[Category: Gao, F]]		[[Category: Gao, F]]
-	[[Category: ~~Verkoczy~~, L]]	+	[[Category: Haynes, B F]]
		+	[[Category: Kelsoe, G]]
		+	[[Category: Keple, T B]]
		+	[[Category: Liao, H X]]
		+	[[Category: Montefiori, D C]]
		+	[[Category: Moody, M A]]
		+	[[Category: Nicely, N I]]
	[[Category: Parks, R]]		[[Category: Parks, R]]
		+	[[Category: Pemble, C]]
		+	[[Category: Santra, S]]
		+	[[Category: Tomaras, G]]
		+	[[Category: Verkoczy, L]]
		+	[[Category: Wiehe, K]]
	[[Category: Zhang, R]]		[[Category: Zhang, R]]
-	[[Category: ~~Kelsoe, G]]~~	+	[[Category: Hiv hiv-1 gp41 mper antibody]]
-	~~[[Category: Keple, T.B]]~~	+	[[Category: Immune system]]
-	~~[[Category: Alam, S.M]]~~	+
-	~~[[Category: Bradley, T]]~~	+
-	~~[[Category: Liao, H.~~-X]]	+
-	[[Category: ~~Nicely, N.I~~]]	+

OCA at 11:05, 16 December 2015

OCA — Wed, 16 Dec 2015 11:05:02 GMT

←Older revision		Revision as of 11:05, 16 December 2015
Line 8:		Line 8:
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: Tomaras, G]]		[[Category: Tomaras, G]]
-	[[Category: Haynes, B.F]]
-	[[Category: Bouton-Verville, H]]
-	[[Category: Santra, S]]
-	[[Category: Montefiori, D.C]]
-	[[Category: Kelsoe, G]]
-	[[Category: Alam, S.M]]
-	[[Category: Liao, H.-X]]
	[[Category: Moody, M.A]]		[[Category: Moody, M.A]]
		+	[[Category: Haynes, B.F]]
	[[Category: Pemble, C]]		[[Category: Pemble, C]]
	[[Category: Foulger, A]]		[[Category: Foulger, A]]
		+	[[Category: Bouton-Verville, H]]
		+	[[Category: Santra, S]]
	[[Category: Wiehe, K]]		[[Category: Wiehe, K]]
		+	[[Category: Montefiori, D.C]]
	[[Category: Gao, F]]		[[Category: Gao, F]]
	[[Category: Verkoczy, L]]		[[Category: Verkoczy, L]]
	[[Category: Parks, R]]		[[Category: Parks, R]]
	[[Category: Zhang, R]]		[[Category: Zhang, R]]
		+	[[Category: Kelsoe, G]]
	[[Category: Keple, T.B]]		[[Category: Keple, T.B]]
		+	[[Category: Alam, S.M]]
	[[Category: Bradley, T]]		[[Category: Bradley, T]]
		+	[[Category: Liao, H.-X]]
	[[Category: Nicely, N.I]]		[[Category: Nicely, N.I]]

OCA at 10:03, 9 September 2015

OCA — Wed, 09 Sep 2015 10:03:10 GMT

←Older revision		Revision as of 10:03, 9 September 2015
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5dd0 is ON HOLD	+	The entry 5dd0 is ON HOLD until Paper Publication

	Authors: Zhang, R., Verkoczy, L., Wiehe, K., Alam, S.M., Nicely, N.I., Santra, S., Bradley, T., Pemble, C., Gao, F., Montefiori, D.C., Bouton-Verville, H., Kelsoe, G., Parks, R., Foulger, A., Tomaras, G., Keple, T.B., Moody, M.A., Liao, H.-X., Haynes, B.F.		Authors: Zhang, R., Verkoczy, L., Wiehe, K., Alam, S.M., Nicely, N.I., Santra, S., Bradley, T., Pemble, C., Gao, F., Montefiori, D.C., Bouton-Verville, H., Kelsoe, G., Parks, R., Foulger, A., Tomaras, G., Keple, T.B., Moody, M.A., Liao, H.-X., Haynes, B.F.

OCA: Protected "5dd0" [edit=sysop:move=sysop]

OCA — Wed, 02 Sep 2015 11:28:50 GMT

Protected "5dd0" [edit=sysop:move=sysop]

←Older revision

Revision as of 11:28, 2 September 2015

OCA: New page: '''Unreleased structure''' The entry 5dd0 is ON HOLD Authors: Zhang, R., Verkoczy, L., Wiehe, K., Alam, S.M., Nicely, N.I., Santra, S., Bradley, T., Pemble, C., Gao, F., Montefiori, D.C...

OCA — Wed, 02 Sep 2015 11:28:49 GMT

New page: '''Unreleased structure''' The entry 5dd0 is ON HOLD Authors: Zhang, R., Verkoczy, L., Wiehe, K., Alam, S.M., Nicely, N.I., Santra, S., Bradley, T., Pemble, C., Gao, F., Montefiori, D.C...

New page

'''Unreleased structure'''

The entry 5dd0 is ON HOLD

Authors: Zhang, R., Verkoczy, L., Wiehe, K., Alam, S.M., Nicely, N.I., Santra, S., Bradley, T., Pemble, C., Gao, F., Montefiori, D.C., Bouton-Verville, H., Kelsoe, G., Parks, R., Foulger, A., Tomaras, G., Keple, T.B., Moody, M.A., Liao, H.-X., Haynes, B.F.

Description:
[[Category: Unreleased Structures]]
[[Category: Tomaras, G]]
[[Category: Haynes, B.F]]
[[Category: Bouton-Verville, H]]
[[Category: Santra, S]]
[[Category: Montefiori, D.C]]
[[Category: Kelsoe, G]]
[[Category: Alam, S.M]]
[[Category: Liao, H.-X]]
[[Category: Moody, M.A]]
[[Category: Pemble, C]]
[[Category: Foulger, A]]
[[Category: Wiehe, K]]
[[Category: Gao, F]]
[[Category: Verkoczy, L]]
[[Category: Parks, R]]
[[Category: Zhang, R]]
[[Category: Keple, T.B]]
[[Category: Bradley, T]]
[[Category: Nicely, N.I]]