5de8 - Revision history

OCA at 12:24, 6 March 2024

OCA — Wed, 06 Mar 2024 12:24:01 GMT

←Older revision		Revision as of 12:24, 6 March 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5de8]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5DE8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5DE8 FirstGlance]. <br>		<table><tr><td colspan='2'>[[5de8]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5DE8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5DE8 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IRI:IRIDIUM+HEXAMMINE+ION'>IRI</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.1003Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IRI:IRIDIUM+HEXAMMINE+ION'>IRI</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5de8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5de8 OCA], [https://pdbe.org/5de8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5de8 RCSB], [https://www.ebi.ac.uk/pdbsum/5de8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5de8 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5de8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5de8 OCA], [https://pdbe.org/5de8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5de8 RCSB], [https://www.ebi.ac.uk/pdbsum/5de8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5de8 ProSAT]</span></td></tr>
	</table>		</table>
Line 11:		Line 12:
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/FMR1_HUMAN FMR1_HUMAN] Translation repressor. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates translation repression (By similarity). RNA-binding protein that plays a role in intracellular RNA transport and in the regulation of translation of target mRNAs. Associated with polysomes. May play a role in the transport of mRNA from the nucleus to the cytoplasm. Binds strongly to poly(G), binds moderately to poly(U) but shows very little binding to poly(A) or poly(C).		[https://www.uniprot.org/uniprot/FMR1_HUMAN FMR1_HUMAN] Translation repressor. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates translation repression (By similarity). RNA-binding protein that plays a role in intracellular RNA transport and in the regulation of translation of target mRNAs. Associated with polysomes. May play a role in the transport of mRNA from the nucleus to the cytoplasm. Binds strongly to poly(G), binds moderately to poly(U) but shows very little binding to poly(A) or poly(C).
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Fragile X Mental Retardation Protein (FMRP) is a regulatory RNA binding protein that plays a central role in the development of several human disorders including Fragile X Syndrome (FXS) and autism. FMRP uses an arginine-glycine-rich (RGG) motif for specific interactions with guanine (G)-quadruplexes, mRNA elements implicated in the disease-associated regulation of specific mRNAs. Here we report the 2.8-A crystal structure of the complex between the human FMRP RGG peptide bound to the in vitro selected G-rich RNA. In this model system, the RNA adopts an intramolecular K+-stabilized G-quadruplex structure composed of three G-quartets and a mixed tetrad connected to an RNA duplex. The RGG peptide specifically binds to the duplex-quadruplex junction, the mixed tetrad, and the duplex region of the RNA through shape complementarity, cation-pi interactions, and multiple hydrogen bonds. Many of these interactions critically depend on a type I beta-turn, a secondary structure element whose formation was not previously recognized in the RGG motif of FMRP. RNA mutagenesis and footprinting experiments indicate that interactions of the peptide with the duplex-quadruplex junction and the duplex of RNA are equally important for affinity and specificity of the RGG-RNA complex formation. These results suggest that specific binding of cellular RNAs by FMRP may involve hydrogen bonding with RNA duplexes and that RNA duplex recognition can be a characteristic RNA binding feature for RGG motifs in other proteins.
-
-	Crystal structure reveals specific recognition of a G-quadruplex RNA by a beta-turn in the RGG motif of FMRP.,Vasilyev N, Polonskaia A, Darnell JC, Darnell RB, Patel DJ, Serganov A Proc Natl Acad Sci U S A. 2015 Sep 15. pii: 201515737. PMID:26374839<ref>PMID:26374839</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 5de8" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 10:31, 21 June 2023

OCA — Wed, 21 Jun 2023 10:31:54 GMT

←Older revision		Revision as of 10:31, 21 June 2023
Line 3:		Line 3:
	<StructureSection load='5de8' size='340' side='right'caption='[[5de8]], [[Resolution\|resolution]] 3.10Å' scene=''>		<StructureSection load='5de8' size='340' side='right'caption='[[5de8]], [[Resolution\|resolution]] 3.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5de8]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5DE8 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5DE8 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5de8]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5DE8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5DE8 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=IRI:IRIDIUM+HEXAMMINE+ION'>IRI</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IRI:IRIDIUM+HEXAMMINE+ION'>IRI</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5de8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5de8 OCA], [~~http~~://pdbe.org/5de8 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5de8 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5de8 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5de8 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5de8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5de8 OCA], [https://pdbe.org/5de8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5de8 RCSB], [https://www.ebi.ac.uk/pdbsum/5de8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5de8 ProSAT]</span></td></tr>
	</table>		</table>
	== Disease ==		== Disease ==
-	[~~[http~~://www.uniprot.org/uniprot/FMR1_HUMAN FMR1_HUMAN]] Defects in FMR1 are the cause of fragile X syndrome (FRAX) [MIM:[~~http~~://omim.org/entry/300624 300624]]. Fragile X syndrome is a common genetic disease (has a prevalence of one in every 2000 children) which is characterized by moderate to severe mental retardation, macroorchidism (enlargement of the testicles), large ears, prominent jaw, and high-pitched, jocular speech. The defect in most fragile X syndrome patients results from an amplification of a CGG repeat region which is directly in front of the coding region.<ref>PMID:18664458</ref> <ref>PMID:8401578</ref> <ref>PMID:7688265</ref> <ref>PMID:17850748</ref> <ref>PMID:8490650</ref> <ref>PMID:7633450</ref> <ref>PMID:9659908</ref> <ref>PMID:15805463</ref> Defects in FMR1 are the cause of fragile X tremor/ataxia syndrome (FXTAS) [MIM:[~~http~~://omim.org/entry/300623 300623]]. In FXTAS, the expanded repeats range in size from 55 to 200 repeats and are referred to as 'premutations'. Full repeat expansions with greater than 200 repeats results in fragile X mental retardation syndrome [MIM:[~~http~~://omim.org/entry/300624 300624]]. Carriers of the premutation typically do not show the full fragile X syndrome phenotype, but comprise a subgroup that may have some physical features of fragile X syndrome or mild cognitive and emotional problems.<ref>PMID:11445641</ref> Defects in FMR1 are the cause of premature ovarian failure syndrome type 1 (POF1) [MIM:[~~http~~://omim.org/entry/311360 311360]]. An ovarian disorder defined as the cessation of ovarian function under the age of 40 years. It is characterized by oligomenorrhea or amenorrhea, in the presence of elevated levels of serum gonadotropins and low estradiol.<ref>PMID:9719368</ref>	+	[https://www.uniprot.org/uniprot/FMR1_HUMAN FMR1_HUMAN] Defects in FMR1 are the cause of fragile X syndrome (FRAX) [MIM:[https://omim.org/entry/300624 300624]. Fragile X syndrome is a common genetic disease (has a prevalence of one in every 2000 children) which is characterized by moderate to severe mental retardation, macroorchidism (enlargement of the testicles), large ears, prominent jaw, and high-pitched, jocular speech. The defect in most fragile X syndrome patients results from an amplification of a CGG repeat region which is directly in front of the coding region.<ref>PMID:18664458</ref> <ref>PMID:8401578</ref> <ref>PMID:7688265</ref> <ref>PMID:17850748</ref> <ref>PMID:8490650</ref> <ref>PMID:7633450</ref> <ref>PMID:9659908</ref> <ref>PMID:15805463</ref> Defects in FMR1 are the cause of fragile X tremor/ataxia syndrome (FXTAS) [MIM:[https://omim.org/entry/300623 300623]. In FXTAS, the expanded repeats range in size from 55 to 200 repeats and are referred to as 'premutations'. Full repeat expansions with greater than 200 repeats results in fragile X mental retardation syndrome [MIM:[https://omim.org/entry/300624 300624]. Carriers of the premutation typically do not show the full fragile X syndrome phenotype, but comprise a subgroup that may have some physical features of fragile X syndrome or mild cognitive and emotional problems.<ref>PMID:11445641</ref> Defects in FMR1 are the cause of premature ovarian failure syndrome type 1 (POF1) [MIM:[https://omim.org/entry/311360 311360]. An ovarian disorder defined as the cessation of ovarian function under the age of 40 years. It is characterized by oligomenorrhea or amenorrhea, in the presence of elevated levels of serum gonadotropins and low estradiol.<ref>PMID:9719368</ref>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/FMR1_HUMAN FMR1_HUMAN]] Translation repressor. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates translation repression (By similarity). RNA-binding protein that plays a role in intracellular RNA transport and in the regulation of translation of target mRNAs. Associated with polysomes. May play a role in the transport of mRNA from the nucleus to the cytoplasm. Binds strongly to poly(G), binds moderately to poly(U) but shows very little binding to poly(A) or poly(C).	+	[https://www.uniprot.org/uniprot/FMR1_HUMAN FMR1_HUMAN] Translation repressor. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates translation repression (By similarity). RNA-binding protein that plays a role in intracellular RNA transport and in the regulation of translation of target mRNAs. Associated with polysomes. May play a role in the transport of mRNA from the nucleus to the cytoplasm. Binds strongly to poly(G), binds moderately to poly(U) but shows very little binding to poly(A) or poly(C).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 24:		Line 24:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Darnell~~, J C~~]]	+	[[Category: Synthetic construct]]
-	[[Category: Darnell~~, R B~~]]	+	[[Category: Darnell JC]]
-	[[Category: Patel~~, D J~~]]	+	[[Category: Darnell RB]]
-	[[Category: Polonskaia, A]]	+	[[Category: Patel DJ]]
-	[[Category: Serganov, A]]	+	[[Category: Polonskaia A]]
-	[[Category: Vasilyev, N]]	+	[[Category: Serganov A]]
-	~~[[Category: Fmrp]]~~	+	[[Category: Vasilyev N]]
-	~~[[Category: Fragile x syndrome]]~~	+
-	~~[[Category: G-quadruplex]]~~	+
-	~~[[Category: Rgg box]]~~	+
-	~~[[Category: Rna binding protein-rna complex]]~~	+
-	~~[[Category: Rna structure~~]]	+

OCA at 07:31, 27 November 2019

OCA — Wed, 27 Nov 2019 07:31:14 GMT

←Older revision		Revision as of 07:31, 27 November 2019
Line 1:		Line 1:

	==Crystal structure of the complex between human FMRP RGG motif and G-quadruplex RNA, iridium hexammine bound form.==		==Crystal structure of the complex between human FMRP RGG motif and G-quadruplex RNA, iridium hexammine bound form.==
-	<StructureSection load='5de8' size='340' side='right' caption='[[5de8]], [[Resolution\|resolution]] 3.10Å' scene=''>	+	<StructureSection load='5de8' size='340' side='right'caption='[[5de8]], [[Resolution\|resolution]] 3.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5de8]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5DE8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5DE8 FirstGlance]. <br>		<table><tr><td colspan='2'>[[5de8]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5DE8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5DE8 FirstGlance]. <br>
Line 24:		Line 24:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Darnell, J C]]		[[Category: Darnell, J C]]
	[[Category: Darnell, R B]]		[[Category: Darnell, R B]]

OCA at 15:51, 16 November 2017

OCA — Thu, 16 Nov 2017 15:51:02 GMT

←Older revision		Revision as of 15:51, 16 November 2017
Line 1:		Line 1:
		+
	==Crystal structure of the complex between human FMRP RGG motif and G-quadruplex RNA, iridium hexammine bound form.==		==Crystal structure of the complex between human FMRP RGG motif and G-quadruplex RNA, iridium hexammine bound form.==
	<StructureSection load='5de8' size='340' side='right' caption='[[5de8]], [[Resolution\|resolution]] 3.10Å' scene=''>		<StructureSection load='5de8' size='340' side='right' caption='[[5de8]], [[Resolution\|resolution]] 3.10Å' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[5de8]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5DE8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5DE8 FirstGlance]. <br>		<table><tr><td colspan='2'>[[5de8]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5DE8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5DE8 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=IRI:IRIDIUM+HEXAMMINE+ION'>IRI</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=IRI:IRIDIUM+HEXAMMINE+ION'>IRI</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5de8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5de8 OCA], [http://pdbe.org/5de8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5de8 RCSB], [http://www.ebi.ac.uk/pdbsum/5de8 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5de8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5de8 OCA], [http://pdbe.org/5de8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5de8 RCSB], [http://www.ebi.ac.uk/pdbsum/5de8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5de8 ProSAT]</span></td></tr>
	</table>		</table>
	== Disease ==		== Disease ==

OCA at 06:58, 30 September 2015

OCA — Wed, 30 Sep 2015 06:58:57 GMT

←Older revision		Revision as of 06:58, 30 September 2015
Line 1:		Line 1:
-	'''~~Unreleased~~ structure'''	+	==Crystal structure of the complex between human FMRP RGG motif and G-quadruplex RNA, iridium hexammine bound form.==
		+	<StructureSection load='5de8' size='340' side='right' caption='[[5de8]], [[Resolution\|resolution]] 3.10Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5de8]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5DE8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5DE8 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=IRI:IRIDIUM+HEXAMMINE+ION'>IRI</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5de8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5de8 OCA], [http://pdbe.org/5de8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5de8 RCSB], [http://www.ebi.ac.uk/pdbsum/5de8 PDBsum]</span></td></tr>
		+	</table>
		+	== Disease ==
		+	[[http://www.uniprot.org/uniprot/FMR1_HUMAN FMR1_HUMAN]] Defects in FMR1 are the cause of fragile X syndrome (FRAX) [MIM:[http://omim.org/entry/300624 300624]]. Fragile X syndrome is a common genetic disease (has a prevalence of one in every 2000 children) which is characterized by moderate to severe mental retardation, macroorchidism (enlargement of the testicles), large ears, prominent jaw, and high-pitched, jocular speech. The defect in most fragile X syndrome patients results from an amplification of a CGG repeat region which is directly in front of the coding region.<ref>PMID:18664458</ref> <ref>PMID:8401578</ref> <ref>PMID:7688265</ref> <ref>PMID:17850748</ref> <ref>PMID:8490650</ref> <ref>PMID:7633450</ref> <ref>PMID:9659908</ref> <ref>PMID:15805463</ref> Defects in FMR1 are the cause of fragile X tremor/ataxia syndrome (FXTAS) [MIM:[http://omim.org/entry/300623 300623]]. In FXTAS, the expanded repeats range in size from 55 to 200 repeats and are referred to as 'premutations'. Full repeat expansions with greater than 200 repeats results in fragile X mental retardation syndrome [MIM:[http://omim.org/entry/300624 300624]]. Carriers of the premutation typically do not show the full fragile X syndrome phenotype, but comprise a subgroup that may have some physical features of fragile X syndrome or mild cognitive and emotional problems.<ref>PMID:11445641</ref> Defects in FMR1 are the cause of premature ovarian failure syndrome type 1 (POF1) [MIM:[http://omim.org/entry/311360 311360]]. An ovarian disorder defined as the cessation of ovarian function under the age of 40 years. It is characterized by oligomenorrhea or amenorrhea, in the presence of elevated levels of serum gonadotropins and low estradiol.<ref>PMID:9719368</ref>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/FMR1_HUMAN FMR1_HUMAN]] Translation repressor. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates translation repression (By similarity). RNA-binding protein that plays a role in intracellular RNA transport and in the regulation of translation of target mRNAs. Associated with polysomes. May play a role in the transport of mRNA from the nucleus to the cytoplasm. Binds strongly to poly(G), binds moderately to poly(U) but shows very little binding to poly(A) or poly(C).
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Fragile X Mental Retardation Protein (FMRP) is a regulatory RNA binding protein that plays a central role in the development of several human disorders including Fragile X Syndrome (FXS) and autism. FMRP uses an arginine-glycine-rich (RGG) motif for specific interactions with guanine (G)-quadruplexes, mRNA elements implicated in the disease-associated regulation of specific mRNAs. Here we report the 2.8-A crystal structure of the complex between the human FMRP RGG peptide bound to the in vitro selected G-rich RNA. In this model system, the RNA adopts an intramolecular K+-stabilized G-quadruplex structure composed of three G-quartets and a mixed tetrad connected to an RNA duplex. The RGG peptide specifically binds to the duplex-quadruplex junction, the mixed tetrad, and the duplex region of the RNA through shape complementarity, cation-pi interactions, and multiple hydrogen bonds. Many of these interactions critically depend on a type I beta-turn, a secondary structure element whose formation was not previously recognized in the RGG motif of FMRP. RNA mutagenesis and footprinting experiments indicate that interactions of the peptide with the duplex-quadruplex junction and the duplex of RNA are equally important for affinity and specificity of the RGG-RNA complex formation. These results suggest that specific binding of cellular RNAs by FMRP may involve hydrogen bonding with RNA duplexes and that RNA duplex recognition can be a characteristic RNA binding feature for RGG motifs in other proteins.

-	~~The entry 5de8 is ON HOLD until Paper Publication~~	+	Crystal structure reveals specific recognition of a G-quadruplex RNA by a beta-turn in the RGG motif of FMRP.,Vasilyev N, Polonskaia A, Darnell JC, Darnell RB, Patel DJ, Serganov A Proc Natl Acad Sci U S A. 2015 Sep 15. pii: 201515737. PMID:26374839<ref>PMID:26374839</ref>

-	~~Authors: Vasilyev~~, N.~~, Polonskaia, A~~.~~, Darnell, J.C., Darnell, R.B., Patel, D.J., Serganov, A~~.	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~Description~~: ~~Crystal structure of the complex between human FMRP RGG motif and G-quadruplex RNA, iridium hexammine bound form.~~	+	<div class="pdbe-citations 5de8" style="background-color:#fffaf0;"></div>
-	[[Category: ~~Unreleased Structures~~]]	+	== References ==
-	[[Category: ~~Patel~~, ~~D.J~~]]	+	<references/>
-	[[Category: ~~Darnell~~, ~~R.B~~]]	+	__TOC__
-	[[Category: ~~Darnell~~, ~~J.C~~]]	+	</StructureSection>
		+	[[Category: Darnell, J C]]
		+	[[Category: Darnell, R B]]
		+	[[Category: Patel, D J]]
		+	[[Category: Polonskaia, A]]
	[[Category: Serganov, A]]		[[Category: Serganov, A]]
-	[[Category: Polonskaia, A]]
	[[Category: Vasilyev, N]]		[[Category: Vasilyev, N]]
		+	[[Category: Fmrp]]
		+	[[Category: Fragile x syndrome]]
		+	[[Category: G-quadruplex]]
		+	[[Category: Rgg box]]
		+	[[Category: Rna binding protein-rna complex]]
		+	[[Category: Rna structure]]

OCA at 10:05, 9 September 2015

OCA — Wed, 09 Sep 2015 10:05:00 GMT

←Older revision		Revision as of 10:05, 9 September 2015
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5de8 is ON HOLD	+	The entry 5de8 is ON HOLD until Paper Publication

	Authors: Vasilyev, N., Polonskaia, A., Darnell, J.C., Darnell, R.B., Patel, D.J., Serganov, A.		Authors: Vasilyev, N., Polonskaia, A., Darnell, J.C., Darnell, R.B., Patel, D.J., Serganov, A.

OCA: Protected "5de8" [edit=sysop:move=sysop]

OCA — Wed, 02 Sep 2015 11:32:08 GMT

Protected "5de8" [edit=sysop:move=sysop]

←Older revision

Revision as of 11:32, 2 September 2015

OCA: New page: '''Unreleased structure''' The entry 5de8 is ON HOLD Authors: Vasilyev, N., Polonskaia, A., Darnell, J.C., Darnell, R.B., Patel, D.J., Serganov, A. Description: Crystal structure of th...

OCA — Wed, 02 Sep 2015 11:32:07 GMT

New page: '''Unreleased structure''' The entry 5de8 is ON HOLD Authors: Vasilyev, N., Polonskaia, A., Darnell, J.C., Darnell, R.B., Patel, D.J., Serganov, A. Description: Crystal structure of th...

New page

'''Unreleased structure'''

The entry 5de8 is ON HOLD

Authors: Vasilyev, N., Polonskaia, A., Darnell, J.C., Darnell, R.B., Patel, D.J., Serganov, A.

Description: Crystal structure of the complex between human FMRP RGG motif and G-quadruplex RNA, iridium hexammine bound form.
[[Category: Unreleased Structures]]
[[Category: Patel, D.J]]
[[Category: Darnell, R.B]]
[[Category: Darnell, J.C]]
[[Category: Serganov, A]]
[[Category: Polonskaia, A]]
[[Category: Vasilyev, N]]