5dqn - Revision history

OCA at 21:55, 28 June 2023

OCA — Wed, 28 Jun 2023 21:55:15 GMT

←Older revision		Revision as of 21:55, 28 June 2023
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	<StructureSection load='5dqn' size='340' side='right'caption='[[5dqn]], [[Resolution\|resolution]] 2.26Å' scene=''>		<StructureSection load='5dqn' size='340' side='right'caption='[[5dqn]], [[Resolution\|resolution]] 2.26Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5dqn]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Mycs2 Mycs2~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5DQN OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5DQN FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5dqn]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycolicibacterium_smegmatis_MC2_155 Mycolicibacterium smegmatis MC2 155]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5DQN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5DQN FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene>, <scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene~~></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.262Å</td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4apy\|4apy]]</td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene>, <scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">cyp125, MSMEI_5834 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=246196 MYCS2])</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5dqn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5dqn OCA], [https://pdbe.org/5dqn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5dqn RCSB], [https://www.ebi.ac.uk/pdbsum/5dqn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5dqn ProSAT]</span></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Linalool_8-monooxygenase Linalool 8-monooxygenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.13.151 1.14.13.151] </span></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5dqn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5dqn OCA], [~~http~~://pdbe.org/5dqn PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5dqn RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5dqn PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5dqn ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/CP125_MYCS2 CP125_MYCS2] Involved in the utilization of cholesterol as the sole carbon and energy source by degrading the side chain. Primarily catalyzes the sequential oxidation of the terminal methyl of cholest-4-en-3-one into (25S)-26-hydroxycholest-4-en-3-one (alcohol), (25S)-26-oxocholest-4-en-3-one (aldehyde), to finally yield the carboxylic acid (25S)-3-oxocholest-4-en-26-oate. Also able to sequentially oxidize cholesterol itself, not only cholest-4-en-3-one.<ref>PMID:23489718</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Linalool 8-monooxygenase]]~~	+	[[Category: Mycolicibacterium smegmatis MC2 155]]
-	~~[[Category: Mycs2~~]]	+	[[Category: Frank DJ]]
-	[[Category: Frank~~, D J~~]]	+	[[Category: Ortiz de Montellano PJ]]
-	[[Category: ~~Montellano, P J.~~Ortiz de]]	+	[[Category: Waddling CA]]
-	[[Category: Waddling~~, C A]]~~	+
-	~~[[Category: Cholesterol metabolism]]~~	+
-	~~[[Category: Oxidoreductase~~]]	+

OCA at 16:04, 11 December 2019

OCA — Wed, 11 Dec 2019 16:04:32 GMT

←Older revision		Revision as of 16:04, 11 December 2019
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	==Polyethylene 600-bound form of P450 CYP125A3 mutant from Myobacterium Smegmatis - W83Y==		==Polyethylene 600-bound form of P450 CYP125A3 mutant from Myobacterium Smegmatis - W83Y==
-	<StructureSection load='5dqn' size='340' side='right' caption='[[5dqn]], [[Resolution\|resolution]] 2.26Å' scene=''>	+	<StructureSection load='5dqn' size='340' side='right'caption='[[5dqn]], [[Resolution\|resolution]] 2.26Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5dqn]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycs2 Mycs2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5DQN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5DQN FirstGlance]. <br>		<table><tr><td colspan='2'>[[5dqn]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycs2 Mycs2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5DQN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5DQN FirstGlance]. <br>
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	</div>		</div>
	<div class="pdbe-citations 5dqn" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5dqn" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Cytochrome P450 3D structures\|Cytochrome P450 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Linalool 8-monooxygenase]]		[[Category: Linalool 8-monooxygenase]]
	[[Category: Mycs2]]		[[Category: Mycs2]]

OCA at 16:56, 15 November 2017

OCA — Wed, 15 Nov 2017 16:56:50 GMT

←Older revision		Revision as of 16:56, 15 November 2017
Line 1:		Line 1:
		+
	==Polyethylene 600-bound form of P450 CYP125A3 mutant from Myobacterium Smegmatis - W83Y==		==Polyethylene 600-bound form of P450 CYP125A3 mutant from Myobacterium Smegmatis - W83Y==
	<StructureSection load='5dqn' size='340' side='right' caption='[[5dqn]], [[Resolution\|resolution]] 2.26Å' scene=''>		<StructureSection load='5dqn' size='340' side='right' caption='[[5dqn]], [[Resolution\|resolution]] 2.26Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5dqn]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5DQN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5DQN FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5dqn]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycs2 Mycs2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5DQN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5DQN FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene>, <scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene>, <scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4apy\|4apy]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4apy\|4apy]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">cyp125, MSMEI_5834 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=246196 MYCS2])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Linalool_8-monooxygenase Linalool 8-monooxygenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.13.151 1.14.13.151] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Linalool_8-monooxygenase Linalool 8-monooxygenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.13.151 1.14.13.151] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5dqn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5dqn OCA], [http://pdbe.org/5dqn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5dqn RCSB], [http://www.ebi.ac.uk/pdbsum/5dqn PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5dqn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5dqn OCA], [http://pdbe.org/5dqn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5dqn RCSB], [http://www.ebi.ac.uk/pdbsum/5dqn PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5dqn ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	</StructureSection>		</StructureSection>
	[[Category: Linalool 8-monooxygenase]]		[[Category: Linalool 8-monooxygenase]]
		+	[[Category: Mycs2]]
	[[Category: Frank, D J]]		[[Category: Frank, D J]]
	[[Category: Montellano, P J.Ortiz de]]		[[Category: Montellano, P J.Ortiz de]]

OCA at 19:01, 1 December 2015

OCA — Tue, 01 Dec 2015 19:01:04 GMT

←Older revision		Revision as of 19:01, 1 December 2015
Line 1:		Line 1:
-	'''~~Unreleased~~ structure'''	+	==Polyethylene 600-bound form of P450 CYP125A3 mutant from Myobacterium Smegmatis - W83Y==
		+	<StructureSection load='5dqn' size='340' side='right' caption='[[5dqn]], [[Resolution\|resolution]] 2.26Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5dqn]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5DQN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5DQN FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene>, <scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4apy\|4apy]]</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Linalool_8-monooxygenase Linalool 8-monooxygenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.13.151 1.14.13.151] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5dqn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5dqn OCA], [http://pdbe.org/5dqn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5dqn RCSB], [http://www.ebi.ac.uk/pdbsum/5dqn PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Mycobacterium tuberculosis (Mtb) and Mycobacterium smegmatis (Msmeg) can grow on cholesterol as the sole carbon source. In Mtb the utilization of cholesterol can be initiated by CYP125A1 or CYP142A1 and in Msmeg by the orthologous CYP125A3 and CYP142A2. Double knockout of the two enzymes in Mtb prevents its growth on cholesterol, but the double knockout of Msmeg is still able to grow, albeit at a slower rate. We report here that Msmeg has a third enzyme, CYP125A4, that also oxidizes cholesterol, although it has a much higher activity for the oxidation of 7alpha-hydroxycholesterol. The ability of Msmeg CYP125A4 (and Mtb CYP125A1) to oxidize 7alpha-hydroxycholesterol is due, at least in part, to the presence of a smaller amino acid side chain facing C-7 of the sterol substrate than in CYP125A3. The ability to oxidize 7-substituted steroids broadens the range of sterol carbon sources for growth, but even more importantly in Mtb, additional biological effects are possible due to the potent immunomodulatory activity of 7alpha,26-dihydroxycholesterol.

-	~~The entry 5dqn is ON HOLD until Paper Publication~~	+	Cytochrome P450 125A4, the Third Cholesterol C-26 Hydroxylase from Mycobacterium smegmatis.,Frank DJ, Waddling CA, La M, Ortiz de Montellano PR Biochemistry. 2015 Nov 24;54(46):6909-16. doi: 10.1021/acs.biochem.5b01029. Epub , 2015 Nov 11. PMID:26522442<ref>PMID:26522442</ref>

-	~~Authors: Ortiz de Montellano~~, P.J.~~, Frank, D.J., Waddling, C.A~~.	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~Description: Polyethylene 600~~-~~bound form of P450 CYP125A3 mutant from Myobacterium Smegmatis~~ -~~W83Y~~	+	<div class="pdbe-citations 5dqn" style="background-color:#fffaf0;"></div>
-	[[Category: ~~Unreleased Structures~~]]	+	== References ==
-	[[Category: Frank, D.J]]	+	<references/>
-	[[Category: Waddling, C.A]]	+	__TOC__
-	[[Category: ~~Ortiz De Montellano, P.J~~]]	+	</StructureSection>
		+	[[Category: Linalool 8-monooxygenase]]
		+	[[Category: Frank, D J]]
		+	[[Category: Montellano, P J.Ortiz de]]
		+	[[Category: Waddling, C A]]
		+	[[Category: Cholesterol metabolism]]
		+	[[Category: Oxidoreductase]]

OCA at 12:30, 7 October 2015

OCA — Wed, 07 Oct 2015 12:30:05 GMT

←Older revision		Revision as of 12:30, 7 October 2015
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5dqn is ON HOLD	+	The entry 5dqn is ON HOLD until Paper Publication

	Authors: Ortiz de Montellano, P.J., Frank, D.J., Waddling, C.A.		Authors: Ortiz de Montellano, P.J., Frank, D.J., Waddling, C.A.

OCA: Protected "5dqn" [edit=sysop:move=sysop]

OCA — Wed, 30 Sep 2015 13:23:04 GMT

Protected "5dqn" [edit=sysop:move=sysop]

←Older revision

Revision as of 13:23, 30 September 2015

OCA: New page: '''Unreleased structure''' The entry 5dqn is ON HOLD Authors: Ortiz de Montellano, P.J., Frank, D.J., Waddling, C.A. Description: Polyethylene 600-bound form of P450 CYP125A3 mutant fr...

OCA — Wed, 30 Sep 2015 13:23:03 GMT

New page: '''Unreleased structure''' The entry 5dqn is ON HOLD Authors: Ortiz de Montellano, P.J., Frank, D.J., Waddling, C.A. Description: Polyethylene 600-bound form of P450 CYP125A3 mutant fr...

New page

'''Unreleased structure'''

The entry 5dqn is ON HOLD

Authors: Ortiz de Montellano, P.J., Frank, D.J., Waddling, C.A.

Description: Polyethylene 600-bound form of P450 CYP125A3 mutant from Myobacterium Smegmatis -W83Y
[[Category: Unreleased Structures]]
[[Category: Frank, D.J]]
[[Category: Waddling, C.A]]
[[Category: Ortiz De Montellano, P.J]]