5dtx - Revision history

OCA at 21:59, 28 June 2023

OCA — Wed, 28 Jun 2023 21:59:03 GMT

←Older revision		Revision as of 21:59, 28 June 2023
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	<StructureSection load='5dtx' size='340' side='right'caption='[[5dtx]], [[Resolution\|resolution]] 1.45Å' scene=''>		<StructureSection load='5dtx' size='340' side='right'caption='[[5dtx]], [[Resolution\|resolution]] 1.45Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5dtx]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Aeqvi Aeqvi~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5DTX OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5DTX FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5dtx]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aequorea_victoria Aequorea victoria]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5DTX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5DTX FirstGlance]. <br>
-	</td></tr><tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non~~-~~Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=PIA:[(4Z)-2-[(1S)-1-AMINOETHYL]-4-(4-HYDROXYBENZYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL]ACETIC+ACID'>PIA</scene>~~</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.45Å</td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GFP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6100 AEQVI])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PIA:[(4Z)-2-[(1S)-1-AMINOETHYL]-4-(4-HYDROXYBENZYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL]ACETIC+ACID'>PIA</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5dtx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5dtx OCA], [~~http~~://pdbe.org/5dtx PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5dtx RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5dtx PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5dtx ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5dtx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5dtx OCA], [https://pdbe.org/5dtx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5dtx RCSB], [https://www.ebi.ac.uk/pdbsum/5dtx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5dtx ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/GFP_AEQVI GFP_AEQVI]] Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.	+	[https://www.uniprot.org/uniprot/GFP_AEQVI GFP_AEQVI] Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Aeqvi~~]]	+	[[Category: Aequorea victoria]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Adam, V]]	+	[[Category: Adam V]]
-	[[Category: Martins, A]]	+	[[Category: Martins A]]
-	~~[[Category: Cis chromophore]]~~	+
-	~~[[Category: Fluorescent protein]]~~	+
-	~~[[Category: Gfp]]~~	+
-	~~[[Category: Reversibly switchable~~]]	+

OCA at 08:05, 15 April 2020

OCA — Wed, 15 Apr 2020 08:05:53 GMT

←Older revision		Revision as of 08:05, 15 April 2020
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		+
	==Crystal structure of rsEGFP2 in the fluorescent on-state==		==Crystal structure of rsEGFP2 in the fluorescent on-state==
-	<StructureSection load='5dtx' size='340' side='right' caption='[[5dtx]], [[Resolution\|resolution]] 1.45Å' scene=''>	+	<StructureSection load='5dtx' size='340' side='right'caption='[[5dtx]], [[Resolution\|resolution]] 1.45Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5dtx]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5DTX OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5DTX FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5dtx]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Aeqvi Aeqvi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5DTX OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5DTX FirstGlance]. <br>
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=PIA:[(4Z)-2-[(1S)-1-AMINOETHYL]-4-(4-HYDROXYBENZYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL]ACETIC+ACID'>PIA</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=PIA:[(4Z)-2-[(1S)-1-AMINOETHYL]-4-(4-HYDROXYBENZYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL]ACETIC+ACID'>PIA</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5dtx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5dtx OCA], [http://pdbe.org/5dtx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5dtx RCSB], [http://www.ebi.ac.uk/pdbsum/5dtx PDBsum]</span></td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GFP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6100 AEQVI])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5dtx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5dtx OCA], [http://pdbe.org/5dtx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5dtx RCSB], [http://www.ebi.ac.uk/pdbsum/5dtx PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5dtx ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	</div>		</div>
	<div class="pdbe-citations 5dtx" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5dtx" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Green Fluorescent Protein 3D structures\|Green Fluorescent Protein 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Aeqvi]]
		+	[[Category: Large Structures]]
	[[Category: Adam, V]]		[[Category: Adam, V]]
	[[Category: Martins, A]]		[[Category: Martins, A]]

OCA at 17:05, 20 January 2016

OCA — Wed, 20 Jan 2016 17:05:41 GMT

←Older revision		Revision as of 17:05, 20 January 2016
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-	'''~~Unreleased~~ structure'''	+	==Crystal structure of rsEGFP2 in the fluorescent on-state==
		+	<StructureSection load='5dtx' size='340' side='right' caption='[[5dtx]], [[Resolution\|resolution]] 1.45Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5dtx]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5DTX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5DTX FirstGlance]. <br>
		+	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=PIA:[(4Z)-2-[(1S)-1-AMINOETHYL]-4-(4-HYDROXYBENZYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL]ACETIC+ACID'>PIA</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5dtx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5dtx OCA], [http://pdbe.org/5dtx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5dtx RCSB], [http://www.ebi.ac.uk/pdbsum/5dtx PDBsum]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/GFP_AEQVI GFP_AEQVI]] Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Phototransformable fluorescent proteins are central to several nanoscopy approaches. As yet however, there is no available variant allowing super-resolution imaging in cell compartments that maintain oxidative conditions. Here, we report the rational design of two reversibly switchable fluorescent proteins able to fold and photoswitch in the bacterial periplasm, rsFolder and rsFolder2. rsFolder was designed by hybridisation of Superfolder-GFP with rsEGFP2, and inherited the fast folding properties of the former together with the rapid switching of the latter, but at the cost of a reduced switching contrast. Structural characterisation of the switching mechanisms of rsFolder and rsEGFP2 revealed different scenarios for chromophore cis-trans isomerisation and allowed designing rsFolder2, a variant of rsFolder that exhibits improved switching contrast and is amenable to RESOLFT nanoscopy. The rsFolders can be efficiently expressed in the E. coli periplasm, opening the door to the nanoscale investigation of proteins localised in hitherto non-observable cellular compartments.

-	~~The entry 5dtx is ON HOLD until Paper Publication~~	+	Rational design of ultrastable and reversibly photoswitchable fluorescent proteins for super-resolution imaging of the bacterial periplasm.,El Khatib M, Martins A, Bourgeois D, Colletier JP, Adam V Sci Rep. 2016 Jan 6;6:18459. doi: 10.1038/srep18459. PMID:26732634<ref>PMID:26732634</ref>

-	~~Authors: Adam~~, V.~~, Martins, A~~.	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~Description: Crystal structure of rsEGFP2 in the fluorescent on~~-~~state~~	+	<div class="pdbe-citations 5dtx" style="background-color:#fffaf0;"></div>
-	~~[[Category~~: ~~Unreleased Structures]]~~	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Adam, V]]		[[Category: Adam, V]]
	[[Category: Martins, A]]		[[Category: Martins, A]]
		+	[[Category: Cis chromophore]]
		+	[[Category: Fluorescent protein]]
		+	[[Category: Gfp]]
		+	[[Category: Reversibly switchable]]

OCA at 04:04, 22 October 2015

OCA — Thu, 22 Oct 2015 04:04:51 GMT

←Older revision		Revision as of 04:04, 22 October 2015
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5dtx is ON HOLD	+	The entry 5dtx is ON HOLD until Paper Publication

	Authors: Adam, V., Martins, A.		Authors: Adam, V., Martins, A.

OCA: Protected "5dtx" [edit=sysop:move=sysop]

OCA — Wed, 30 Sep 2015 13:31:28 GMT

Protected "5dtx" [edit=sysop:move=sysop]

←Older revision

Revision as of 13:31, 30 September 2015

OCA: New page: '''Unreleased structure''' The entry 5dtx is ON HOLD Authors: Adam, V., Martins, A. Description: Crystal structure of rsEGFP2 in the fluorescent on-state [[Category: Unreleased Structu...

OCA — Wed, 30 Sep 2015 13:31:27 GMT

New page: '''Unreleased structure''' The entry 5dtx is ON HOLD Authors: Adam, V., Martins, A. Description: Crystal structure of rsEGFP2 in the fluorescent on-state [[Category: Unreleased Structu...

New page

'''Unreleased structure'''

The entry 5dtx is ON HOLD

Authors: Adam, V., Martins, A.

Description: Crystal structure of rsEGFP2 in the fluorescent on-state
[[Category: Unreleased Structures]]
[[Category: Adam, V]]
[[Category: Martins, A]]