5dty - Revision history

OCA at 21:59, 28 June 2023

OCA — Wed, 28 Jun 2023 21:59:06 GMT

←Older revision		Revision as of 21:59, 28 June 2023
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	<StructureSection load='5dty' size='340' side='right'caption='[[5dty]], [[Resolution\|resolution]] 1.50Å' scene=''>		<StructureSection load='5dty' size='340' side='right'caption='[[5dty]], [[Resolution\|resolution]] 1.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5dty]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Aeqvi Aeqvi~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5DTY OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5DTY FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5dty]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aequorea_victoria Aequorea victoria]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5DTY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5DTY FirstGlance]. <br>
-	</td></tr><tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non~~-~~Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=PIA:[(4Z)-2-[(1S)-1-AMINOETHYL]-4-(4-HYDROXYBENZYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL]ACETIC+ACID'>PIA</scene>~~</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.5Å</td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GFP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6100 AEQVI])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PIA:[(4Z)-2-[(1S)-1-AMINOETHYL]-4-(4-HYDROXYBENZYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL]ACETIC+ACID'>PIA</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5dty FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5dty OCA], [~~http~~://pdbe.org/5dty PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5dty RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5dty PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5dty ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5dty FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5dty OCA], [https://pdbe.org/5dty PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5dty RCSB], [https://www.ebi.ac.uk/pdbsum/5dty PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5dty ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/GFP_AEQVI GFP_AEQVI]] Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.	+	[https://www.uniprot.org/uniprot/GFP_AEQVI GFP_AEQVI] Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Aeqvi~~]]	+	[[Category: Aequorea victoria]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Adam, V]]	+	[[Category: Adam V]]
-	[[Category: ~~Martins, A~~ Dos-Santos]]	+	[[Category: Dos-Santos Martins A]]
-	~~[[Category: Fluorescent protein]]~~	+
-	~~[[Category: Gfp]]~~	+
-	~~[[Category: Reversibly switchable]]~~	+
-	~~[[Category: Trans chromophore~~]]	+

OCA at 08:05, 15 April 2020

OCA — Wed, 15 Apr 2020 08:05:58 GMT

←Older revision		Revision as of 08:05, 15 April 2020
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		+
	==Crystal structure of rsEGFP2 in the non-fluorescent off-state==		==Crystal structure of rsEGFP2 in the non-fluorescent off-state==
-	<StructureSection load='5dty' size='340' side='right' caption='[[5dty]], [[Resolution\|resolution]] 1.50Å' scene=''>	+	<StructureSection load='5dty' size='340' side='right'caption='[[5dty]], [[Resolution\|resolution]] 1.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5dty]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5DTY OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5DTY FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5dty]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Aeqvi Aeqvi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5DTY OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5DTY FirstGlance]. <br>
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=PIA:[(4Z)-2-[(1S)-1-AMINOETHYL]-4-(4-HYDROXYBENZYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL]ACETIC+ACID'>PIA</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=PIA:[(4Z)-2-[(1S)-1-AMINOETHYL]-4-(4-HYDROXYBENZYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL]ACETIC+ACID'>PIA</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5dty FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5dty OCA], [http://pdbe.org/5dty PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5dty RCSB], [http://www.ebi.ac.uk/pdbsum/5dty PDBsum]</span></td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GFP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6100 AEQVI])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5dty FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5dty OCA], [http://pdbe.org/5dty PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5dty RCSB], [http://www.ebi.ac.uk/pdbsum/5dty PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5dty ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	</div>		</div>
	<div class="pdbe-citations 5dty" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5dty" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Green Fluorescent Protein 3D structures\|Green Fluorescent Protein 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Aeqvi]]
		+	[[Category: Large Structures]]
	[[Category: Adam, V]]		[[Category: Adam, V]]
	[[Category: Martins, A Dos-Santos]]		[[Category: Martins, A Dos-Santos]]

OCA at 16:56, 20 January 2016

OCA — Wed, 20 Jan 2016 16:56:50 GMT

←Older revision		Revision as of 16:56, 20 January 2016
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-	'''~~Unreleased~~ structure'''	+	==Crystal structure of rsEGFP2 in the non-fluorescent off-state==
		+	<StructureSection load='5dty' size='340' side='right' caption='[[5dty]], [[Resolution\|resolution]] 1.50Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5dty]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5DTY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5DTY FirstGlance]. <br>
		+	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=PIA:[(4Z)-2-[(1S)-1-AMINOETHYL]-4-(4-HYDROXYBENZYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL]ACETIC+ACID'>PIA</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5dty FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5dty OCA], [http://pdbe.org/5dty PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5dty RCSB], [http://www.ebi.ac.uk/pdbsum/5dty PDBsum]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/GFP_AEQVI GFP_AEQVI]] Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Phototransformable fluorescent proteins are central to several nanoscopy approaches. As yet however, there is no available variant allowing super-resolution imaging in cell compartments that maintain oxidative conditions. Here, we report the rational design of two reversibly switchable fluorescent proteins able to fold and photoswitch in the bacterial periplasm, rsFolder and rsFolder2. rsFolder was designed by hybridisation of Superfolder-GFP with rsEGFP2, and inherited the fast folding properties of the former together with the rapid switching of the latter, but at the cost of a reduced switching contrast. Structural characterisation of the switching mechanisms of rsFolder and rsEGFP2 revealed different scenarios for chromophore cis-trans isomerisation and allowed designing rsFolder2, a variant of rsFolder that exhibits improved switching contrast and is amenable to RESOLFT nanoscopy. The rsFolders can be efficiently expressed in the E. coli periplasm, opening the door to the nanoscale investigation of proteins localised in hitherto non-observable cellular compartments.

-	~~The entry 5dty is ON HOLD until Paper Publication~~	+	Rational design of ultrastable and reversibly photoswitchable fluorescent proteins for super-resolution imaging of the bacterial periplasm.,El Khatib M, Martins A, Bourgeois D, Colletier JP, Adam V Sci Rep. 2016 Jan 6;6:18459. doi: 10.1038/srep18459. PMID:26732634<ref>PMID:26732634</ref>

-	~~Authors: Adam~~, V.~~, Dos-Santos Martins, A~~.	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~Description: Crystal structure of rsEGFP2 in the non~~-~~fluorescent off~~-~~state~~	+	<div class="pdbe-citations 5dty" style="background-color:#fffaf0;"></div>
-	~~[[Category~~: ~~Unreleased Structures]]~~	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Adam, V]]		[[Category: Adam, V]]
-	[[Category: ~~Dos-Santos~~ Martins, A]]	+	[[Category: Martins, A Dos-Santos]]
		+	[[Category: Fluorescent protein]]
		+	[[Category: Gfp]]
		+	[[Category: Reversibly switchable]]
		+	[[Category: Trans chromophore]]

OCA at 02:10, 1 December 2015

OCA — Tue, 01 Dec 2015 02:10:33 GMT

←Older revision		Revision as of 02:10, 1 December 2015
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5dty is ON HOLD	+	The entry 5dty is ON HOLD until Paper Publication

	Authors: Adam, V., Dos-Santos Martins, A.		Authors: Adam, V., Dos-Santos Martins, A.

OCA: Protected "5dty" [edit=sysop:move=sysop]

OCA — Wed, 30 Sep 2015 13:31:30 GMT

Protected "5dty" [edit=sysop:move=sysop]

←Older revision

Revision as of 13:31, 30 September 2015

OCA: New page: '''Unreleased structure''' The entry 5dty is ON HOLD Authors: Adam, V., Dos-Santos Martins, A. Description: Crystal structure of rsEGFP2 in the non-fluorescent off-state [[Category: Un...

OCA — Wed, 30 Sep 2015 13:31:29 GMT

New page: '''Unreleased structure''' The entry 5dty is ON HOLD Authors: Adam, V., Dos-Santos Martins, A. Description: Crystal structure of rsEGFP2 in the non-fluorescent off-state [[Category: Un...

New page

'''Unreleased structure'''

The entry 5dty is ON HOLD

Authors: Adam, V., Dos-Santos Martins, A.

Description: Crystal structure of rsEGFP2 in the non-fluorescent off-state
[[Category: Unreleased Structures]]
[[Category: Adam, V]]
[[Category: Dos-Santos Martins, A]]