5dxf - Revision history

OCA at 22:04, 28 June 2023

OCA — Wed, 28 Jun 2023 22:04:18 GMT

←Older revision		Revision as of 22:04, 28 June 2023
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	<StructureSection load='5dxf' size='340' side='right'caption='[[5dxf]], [[Resolution\|resolution]] 2.56Å' scene=''>		<StructureSection load='5dxf' size='340' side='right'caption='[[5dxf]], [[Resolution\|resolution]] 2.56Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5dxf]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Canal Canal~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5DXF OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5DXF FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5dxf]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Candida_albicans_SC5314 Candida albicans SC5314]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5DXF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5DXF FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~>[[5dxi\|5dxi]], [[5dx9\|5dx9]], [[5dxl\|5dxl]], [[5dxn\|5dxn]], [[5dxo\|5dxo]]</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.563Å</td></tr>
-	~~<tr~~ id~~='gene'><td class~~="~~sblockLbl~~">~~<b>~~[[~~Gene~~\|~~Gene:~~]]~~</b></td><td class="sblockDat">TPS2, CaO19~~.~~10556, CaO19.3038, I503_00327, W5Q_00328 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=237561 CANAL])</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5dxf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5dxf OCA], [https://pdbe.org/5dxf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5dxf RCSB], [https://www.ebi.ac.uk/pdbsum/5dxf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5dxf ProSAT]</span></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Alpha,alpha-trehalose-phosphate_synthase_(UDP-forming) Alpha,alpha-trehalose-phosphate synthase (UDP-forming)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.15 2.4.1.15] </span></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5dxf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5dxf OCA], [~~http~~://pdbe.org/5dxf PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5dxf RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5dxf PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5dxf ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q5AI14_CANAL Q5AI14_CANAL]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Canal~~]]	+	[[Category: Candida albicans SC5314]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Brennan~~, R G~~]]	+	[[Category: Brennan RG]]
-	[[Category: Miao, Y]]	+	[[Category: Miao Y]]
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Trehalose-6-phosphate phosphatase~~]]	+

OCA at 16:04, 11 December 2019

OCA — Wed, 11 Dec 2019 16:04:57 GMT

←Older revision		Revision as of 16:04, 11 December 2019
Line 1:		Line 1:

	==Structure of Candida albicans trehalose-6-phosphate phosphatase N-terminal domain==		==Structure of Candida albicans trehalose-6-phosphate phosphatase N-terminal domain==
-	<StructureSection load='5dxf' size='340' side='right' caption='[[5dxf]], [[Resolution\|resolution]] 2.56Å' scene=''>	+	<StructureSection load='5dxf' size='340' side='right'caption='[[5dxf]], [[Resolution\|resolution]] 2.56Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5dxf]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5DXF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5DXF FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5dxf]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Canal Canal]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5DXF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5DXF FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5dxi\|5dxi]], [[5dx9\|5dx9]], [[5dxl\|5dxl]], [[5dxn\|5dxn]], [[5dxo\|5dxo]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5dxi\|5dxi]], [[5dx9\|5dx9]], [[5dxl\|5dxl]], [[5dxn\|5dxn]], [[5dxo\|5dxo]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">TPS2, CaO19.10556, CaO19.3038, I503_00327, W5Q_00328 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=237561 CANAL])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Alpha,alpha-trehalose-phosphate_synthase_(UDP-forming) Alpha,alpha-trehalose-phosphate synthase (UDP-forming)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.15 2.4.1.15] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Alpha,alpha-trehalose-phosphate_synthase_(UDP-forming) Alpha,alpha-trehalose-phosphate synthase (UDP-forming)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.15 2.4.1.15] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5dxf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5dxf OCA], [http://pdbe.org/5dxf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5dxf RCSB], [http://www.ebi.ac.uk/pdbsum/5dxf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5dxf ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5dxf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5dxf OCA], [http://pdbe.org/5dxf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5dxf RCSB], [http://www.ebi.ac.uk/pdbsum/5dxf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5dxf ProSAT]</span></td></tr>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Canal]]
		+	[[Category: Large Structures]]
	[[Category: Brennan, R G]]		[[Category: Brennan, R G]]
	[[Category: Miao, Y]]		[[Category: Miao, Y]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
	[[Category: Trehalose-6-phosphate phosphatase]]		[[Category: Trehalose-6-phosphate phosphatase]]

OCA at 18:29, 12 July 2016

OCA — Tue, 12 Jul 2016 18:29:43 GMT

←Older revision		Revision as of 18:29, 12 July 2016
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	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5dxi\|5dxi]], [[5dx9\|5dx9]], [[5dxl\|5dxl]], [[5dxn\|5dxn]], [[5dxo\|5dxo]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5dxi\|5dxi]], [[5dx9\|5dx9]], [[5dxl\|5dxl]], [[5dxn\|5dxn]], [[5dxo\|5dxo]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Alpha,alpha-trehalose-phosphate_synthase_(UDP-forming) Alpha,alpha-trehalose-phosphate synthase (UDP-forming)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.15 2.4.1.15] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Alpha,alpha-trehalose-phosphate_synthase_(UDP-forming) Alpha,alpha-trehalose-phosphate synthase (UDP-forming)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.15 2.4.1.15] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5dxf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5dxf OCA], [http://pdbe.org/5dxf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5dxf RCSB], [http://www.ebi.ac.uk/pdbsum/5dxf PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5dxf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5dxf OCA], [http://pdbe.org/5dxf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5dxf RCSB], [http://www.ebi.ac.uk/pdbsum/5dxf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5dxf ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Trehalose is a disaccharide essential for the survival and virulence of pathogenic fungi. The biosynthesis of trehalose requires trehalose-6-phosphate synthase, Tps1, and trehalose-6-phosphate phosphatase, Tps2. Here, we report the structures of the N-terminal domain of Tps2 (Tps2NTD) from Candida albicans, a transition-state complex of the Tps2 C-terminal trehalose-6-phosphate phosphatase domain (Tps2PD) bound to BeF3 and trehalose, and catalytically dead Tps2PD(D24N) from Cryptococcus neoformans bound to trehalose-6-phosphate (T6P). The Tps2NTD closely resembles the structure of Tps1 but lacks any catalytic activity. The Tps2PD-BeF3-trehalose and Tps2PD(D24N)-T6P complex structures reveal a "closed" conformation that is effected by extensive interactions between each trehalose hydroxyl group and residues of the cap and core domains of the protein, thereby providing exquisite substrate specificity. Disruption of any of the direct substrate-protein residue interactions leads to significant or complete loss of phosphatase activity. Notably, the Tps2PD-BeF3-trehalose complex structure captures an aspartyl-BeF3 covalent adduct, which closely mimics the proposed aspartyl-phosphate intermediate of the phosphatase catalytic cycle. Structures of substrate-free Tps2PD reveal an "open" conformation whereby the cap and core domains separate and visualize the striking conformational changes effected by substrate binding and product release and the role of two hinge regions centered at approximately residues 102-103 and 184-188. Significantly, tps2Delta, tps2NTDDelta, and tps2D705N strains are unable to grow at elevated temperatures. Combined, these studies provide a deeper understanding of the substrate recognition and catalytic mechanism of Tps2 and provide a structural basis for the future design of novel antifungal compounds against a target found in three major fungal pathogens.
		+
		+	Structures of trehalose-6-phosphate phosphatase from pathogenic fungi reveal the mechanisms of substrate recognition and catalysis.,Miao Y, Tenor JL, Toffaletti DL, Washington EJ, Liu J, Shadrick WR, Schumacher MA, Lee RE, Perfect JR, Brennan RG Proc Natl Acad Sci U S A. 2016 Jun 28;113(26):7148-53. doi:, 10.1073/pnas.1601774113. Epub 2016 Jun 15. PMID:27307435<ref>PMID:27307435</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5dxf" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 22:23, 20 June 2016

OCA — Mon, 20 Jun 2016 22:23:51 GMT

←Older revision		Revision as of 22:23, 20 June 2016
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5dxf ~~is ON HOLD until Paper Publication~~	+	==Structure of Candida albicans trehalose-6-phosphate phosphatase N-terminal domain==
-		+	<StructureSection load='5dxf' size='340' side='right' caption='[[5dxf]], [[Resolution\|resolution]] 2.56Å' scene=''>
-	~~Authors~~: ~~Miao, Y~~.~~, Brennan, R~~.G.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5dxf]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5DXF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5DXF FirstGlance]. <br>
-	~~Description~~: ~~Structure of Candida albicans~~ trehalose-6-phosphate ~~phosphatase N~~-~~terminal domain~~	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5dxi\|5dxi]], [[5dx9\|5dx9]], [[5dxl\|5dxl]], [[5dxn\|5dxn]], [[5dxo\|5dxo]]</td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Alpha,alpha-trehalose-phosphate_synthase_(UDP-forming) Alpha,alpha-trehalose-phosphate synthase (UDP-forming)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.15 2.4.1.15] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5dxf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5dxf OCA], [http://pdbe.org/5dxf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5dxf RCSB], [http://www.ebi.ac.uk/pdbsum/5dxf PDBsum]</span></td></tr>
		+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Brennan, R G]]
	[[Category: Miao, Y]]		[[Category: Miao, Y]]
-	[[Category: ~~Brennan, R.G~~]]	+	[[Category: Hydrolase]]
		+	[[Category: Trehalose-6-phosphate phosphatase]]

OCA at 11:34, 3 February 2016

OCA — Wed, 03 Feb 2016 11:34:48 GMT

←Older revision		Revision as of 11:34, 3 February 2016
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5dxf is ON HOLD	+	The entry 5dxf is ON HOLD until Paper Publication

	Authors: Miao, Y., Brennan, R.G.		Authors: Miao, Y., Brennan, R.G.

OCA at 12:40, 7 October 2015

OCA — Wed, 07 Oct 2015 12:40:52 GMT

←Older revision		Revision as of 12:40, 7 October 2015
Line 3:		Line 3:
	The entry 5dxf is ON HOLD		The entry 5dxf is ON HOLD

-	Authors: Miao, Y, Brennan, R.G.	+	Authors: Miao, Y., Brennan, R.G.

	Description: Structure of Candida albicans trehalose-6-phosphate phosphatase N-terminal domain		Description: Structure of Candida albicans trehalose-6-phosphate phosphatase N-terminal domain
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Miao, Y, Brennan, R.G]]	+	[[Category: Miao, Y]]
		+	[[Category: Brennan, R.G]]

OCA: Protected "5dxf" [edit=sysop:move=sysop]

OCA — Wed, 30 Sep 2015 13:39:15 GMT

Protected "5dxf" [edit=sysop:move=sysop]

←Older revision

Revision as of 13:39, 30 September 2015

OCA: New page: '''Unreleased structure''' The entry 5dxf is ON HOLD Authors: Miao, Y, Brennan, R.G. Description: Structure of Candida albicans trehalose-6-phosphate phosphatase N-terminal domain [[Ca...

OCA — Wed, 30 Sep 2015 13:39:13 GMT

New page: '''Unreleased structure''' The entry 5dxf is ON HOLD Authors: Miao, Y, Brennan, R.G. Description: Structure of Candida albicans trehalose-6-phosphate phosphatase N-terminal domain [[Ca...

New page

'''Unreleased structure'''

The entry 5dxf is ON HOLD

Authors: Miao, Y, Brennan, R.G.

Description: Structure of Candida albicans trehalose-6-phosphate phosphatase N-terminal domain
[[Category: Unreleased Structures]]
[[Category: Miao, Y, Brennan, R.G]]