5dxn - Revision history

OCA at 22:04, 28 June 2023

OCA — Wed, 28 Jun 2023 22:04:32 GMT

←Older revision		Revision as of 22:04, 28 June 2023
Line 3:		Line 3:
	<StructureSection load='5dxn' size='340' side='right'caption='[[5dxn]], [[Resolution\|resolution]] 1.65Å' scene=''>		<StructureSection load='5dxn' size='340' side='right'caption='[[5dxn]], [[Resolution\|resolution]] 1.65Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5dxn]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Aspfu Aspfu~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5DXN OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5DXN FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5dxn]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aspergillus_fumigatus_Af293 Aspergillus fumigatus Af293]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5DXN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5DXN FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat"~~><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.65Å</td></tr>
-	~~<tr~~ id~~='related'><td class~~="~~sblockLbl~~">~~<b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5dxi\|5dxi]]~~, [[~~5dx9~~\|~~5dx9]], [[5dxf\|5dxf]], [[5dxl\|5dxl]], [[5dxo\|5dxo~~]]</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>AFUA_3G05650 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~330879 ASPFU])</td~~><~~/tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5dxn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5dxn OCA], [https://pdbe.org/5dxn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5dxn RCSB], [https://www.ebi.ac.uk/pdbsum/5dxn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5dxn ProSAT]</span></td></tr>
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b></td><td class="sblockDat"><span class='plainlinks~~'>[http://en.wikipedia.org/wiki/Alpha,alpha-trehalose-phosphate_synthase_(UDP-forming) Alpha,alpha-trehalose-phosphate synthase (UDP-forming)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.15 2.4.1.15] </~~span~~></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5dxn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5dxn OCA], [~~http~~://pdbe.org/5dxn PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5dxn RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5dxn PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5dxn ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q4WWF5_ASPFU Q4WWF5_ASPFU]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 23:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Aspfu~~]]	+	[[Category: Aspergillus fumigatus Af293]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Brennan~~, R G~~]]	+	[[Category: Brennan RG]]
-	[[Category: Miao, Y]]	+	[[Category: Miao Y]]
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Phosphatase]]~~	+
-	~~[[Category: Trehalose-6-phosphate~~]]	+

OCA at 16:05, 11 December 2019

OCA — Wed, 11 Dec 2019 16:05:12 GMT

←Older revision		Revision as of 16:05, 11 December 2019
Line 1:		Line 1:

	==Structure of Aspergillus fumigatus trehalose-6-phosphate phosphatase crystal form 2==		==Structure of Aspergillus fumigatus trehalose-6-phosphate phosphatase crystal form 2==
-	<StructureSection load='5dxn' size='340' side='right' caption='[[5dxn]], [[Resolution\|resolution]] 1.65Å' scene=''>	+	<StructureSection load='5dxn' size='340' side='right'caption='[[5dxn]], [[Resolution\|resolution]] 1.65Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5dxn]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5DXN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5DXN FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5dxn]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Aspfu Aspfu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5DXN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5DXN FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5dxi\|5dxi]], [[5dx9\|5dx9]], [[5dxf\|5dxf]], [[5dxl\|5dxl]], [[5dxo\|5dxo]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5dxi\|5dxi]], [[5dx9\|5dx9]], [[5dxf\|5dxf]], [[5dxl\|5dxl]], [[5dxo\|5dxo]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">AFUA_3G05650 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=330879 ASPFU])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Alpha,alpha-trehalose-phosphate_synthase_(UDP-forming) Alpha,alpha-trehalose-phosphate synthase (UDP-forming)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.15 2.4.1.15] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Alpha,alpha-trehalose-phosphate_synthase_(UDP-forming) Alpha,alpha-trehalose-phosphate synthase (UDP-forming)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.15 2.4.1.15] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5dxn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5dxn OCA], [http://pdbe.org/5dxn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5dxn RCSB], [http://www.ebi.ac.uk/pdbsum/5dxn PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5dxn ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5dxn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5dxn OCA], [http://pdbe.org/5dxn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5dxn RCSB], [http://www.ebi.ac.uk/pdbsum/5dxn PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5dxn ProSAT]</span></td></tr>
Line 22:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Aspfu]]
		+	[[Category: Large Structures]]
	[[Category: Brennan, R G]]		[[Category: Brennan, R G]]
	[[Category: Miao, Y]]		[[Category: Miao, Y]]

OCA at 02:54, 13 July 2016

OCA — Wed, 13 Jul 2016 02:54:24 GMT

←Older revision		Revision as of 02:54, 13 July 2016
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	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5dxi\|5dxi]], [[5dx9\|5dx9]], [[5dxf\|5dxf]], [[5dxl\|5dxl]], [[5dxo\|5dxo]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5dxi\|5dxi]], [[5dx9\|5dx9]], [[5dxf\|5dxf]], [[5dxl\|5dxl]], [[5dxo\|5dxo]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Alpha,alpha-trehalose-phosphate_synthase_(UDP-forming) Alpha,alpha-trehalose-phosphate synthase (UDP-forming)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.15 2.4.1.15] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Alpha,alpha-trehalose-phosphate_synthase_(UDP-forming) Alpha,alpha-trehalose-phosphate synthase (UDP-forming)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.15 2.4.1.15] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5dxn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5dxn OCA], [http://pdbe.org/5dxn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5dxn RCSB], [http://www.ebi.ac.uk/pdbsum/5dxn PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5dxn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5dxn OCA], [http://pdbe.org/5dxn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5dxn RCSB], [http://www.ebi.ac.uk/pdbsum/5dxn PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5dxn ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Trehalose is a disaccharide essential for the survival and virulence of pathogenic fungi. The biosynthesis of trehalose requires trehalose-6-phosphate synthase, Tps1, and trehalose-6-phosphate phosphatase, Tps2. Here, we report the structures of the N-terminal domain of Tps2 (Tps2NTD) from Candida albicans, a transition-state complex of the Tps2 C-terminal trehalose-6-phosphate phosphatase domain (Tps2PD) bound to BeF3 and trehalose, and catalytically dead Tps2PD(D24N) from Cryptococcus neoformans bound to trehalose-6-phosphate (T6P). The Tps2NTD closely resembles the structure of Tps1 but lacks any catalytic activity. The Tps2PD-BeF3-trehalose and Tps2PD(D24N)-T6P complex structures reveal a "closed" conformation that is effected by extensive interactions between each trehalose hydroxyl group and residues of the cap and core domains of the protein, thereby providing exquisite substrate specificity. Disruption of any of the direct substrate-protein residue interactions leads to significant or complete loss of phosphatase activity. Notably, the Tps2PD-BeF3-trehalose complex structure captures an aspartyl-BeF3 covalent adduct, which closely mimics the proposed aspartyl-phosphate intermediate of the phosphatase catalytic cycle. Structures of substrate-free Tps2PD reveal an "open" conformation whereby the cap and core domains separate and visualize the striking conformational changes effected by substrate binding and product release and the role of two hinge regions centered at approximately residues 102-103 and 184-188. Significantly, tps2Delta, tps2NTDDelta, and tps2D705N strains are unable to grow at elevated temperatures. Combined, these studies provide a deeper understanding of the substrate recognition and catalytic mechanism of Tps2 and provide a structural basis for the future design of novel antifungal compounds against a target found in three major fungal pathogens.
		+
		+	Structures of trehalose-6-phosphate phosphatase from pathogenic fungi reveal the mechanisms of substrate recognition and catalysis.,Miao Y, Tenor JL, Toffaletti DL, Washington EJ, Liu J, Shadrick WR, Schumacher MA, Lee RE, Perfect JR, Brennan RG Proc Natl Acad Sci U S A. 2016 Jun 28;113(26):7148-53. doi:, 10.1073/pnas.1601774113. Epub 2016 Jun 15. PMID:27307435<ref>PMID:27307435</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5dxn" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 22:24, 20 June 2016

OCA — Mon, 20 Jun 2016 22:24:02 GMT

←Older revision		Revision as of 22:24, 20 June 2016
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5dxn ~~is ON HOLD until Paper Publication~~	+	==Structure of Aspergillus fumigatus trehalose-6-phosphate phosphatase crystal form 2==
-		+	<StructureSection load='5dxn' size='340' side='right' caption='[[5dxn]], [[Resolution\|resolution]] 1.65Å' scene=''>
-	~~Authors~~: ~~Miao, Y~~.~~, Brennan, R~~.G.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5dxn]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5DXN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5DXN FirstGlance]. <br>
-	~~Description~~: ~~Structure of Aspergillus fumigatus~~ trehalose-6-phosphate ~~phosphatase crystal form~~ 2	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5dxi\|5dxi]], [[5dx9\|5dx9]], [[5dxf\|5dxf]], [[5dxl\|5dxl]], [[5dxo\|5dxo]]</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Alpha,alpha-trehalose-phosphate_synthase_(UDP-forming) Alpha,alpha-trehalose-phosphate synthase (UDP-forming)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.15 2.4.1.15] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5dxn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5dxn OCA], [http://pdbe.org/5dxn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5dxn RCSB], [http://www.ebi.ac.uk/pdbsum/5dxn PDBsum]</span></td></tr>
		+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Brennan, R G]]
	[[Category: Miao, Y]]		[[Category: Miao, Y]]
-	[[Category: ~~Brennan, R.G~~]]	+	[[Category: Hydrolase]]
		+	[[Category: Phosphatase]]
		+	[[Category: Trehalose-6-phosphate]]

OCA at 11:35, 3 February 2016

OCA — Wed, 03 Feb 2016 11:35:06 GMT

←Older revision		Revision as of 11:35, 3 February 2016
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5dxn is ON HOLD	+	The entry 5dxn is ON HOLD until Paper Publication

	Authors: Miao, Y., Brennan, R.G.		Authors: Miao, Y., Brennan, R.G.

OCA: Protected "5dxn" [edit=sysop:move=sysop]

OCA — Wed, 30 Sep 2015 13:40:33 GMT

Protected "5dxn" [edit=sysop:move=sysop]

←Older revision

Revision as of 13:40, 30 September 2015

OCA: New page: '''Unreleased structure''' The entry 5dxn is ON HOLD Authors: Miao, Y., Brennan, R.G. Description: Structure of Aspergillus fumigatus trehalose-6-phosphate phosphatase crystal form 2 [...

OCA — Wed, 30 Sep 2015 13:40:31 GMT

New page: '''Unreleased structure''' The entry 5dxn is ON HOLD Authors: Miao, Y., Brennan, R.G. Description: Structure of Aspergillus fumigatus trehalose-6-phosphate phosphatase crystal form 2 [...

New page

'''Unreleased structure'''

The entry 5dxn is ON HOLD

Authors: Miao, Y., Brennan, R.G.

Description: Structure of Aspergillus fumigatus trehalose-6-phosphate phosphatase crystal form 2
[[Category: Unreleased Structures]]
[[Category: Miao, Y]]
[[Category: Brennan, R.G]]