5dyj - Revision history

OCA at 22:05, 28 June 2023

OCA — Wed, 28 Jun 2023 22:05:49 GMT

←Older revision		Revision as of 22:05, 28 June 2023
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	<StructureSection load='5dyj' size='340' side='right'caption='[[5dyj]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='5dyj' size='340' side='right'caption='[[5dyj]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5dyj]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Dicdi Dicdi~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5DYJ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5DYJ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5dyj]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Dictyostelium_discoideum Dictyostelium discoideum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5DYJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5DYJ FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene>~~, ~~<scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.5Å</td></tr>
-	<tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=~~TPO~~:~~PHOSPHOTHREONINE~~'>~~TPO~~</scene><~~/td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=TPO:PHOSPHOTHREONINE'>TPO</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	~~<tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b~~></td><~~td class~~=~~"sblockDat">mhkA, mhckA, DDB_G0291231 ([http~~:~~//www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=44689 DICDI])</td~~></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5dyj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5dyj OCA], [https://pdbe.org/5dyj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5dyj RCSB], [https://www.ebi.ac.uk/pdbsum/5dyj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5dyj ProSAT]</span></td></tr>
-	<~~tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b~~></td><~~td class~~=~~"sblockDat"><span class~~=~~'plainlinks~~'>~~[http://en.wikipedia.org/wiki/[Myosin_heavy-chain]_kinase [Myosin heavy-chain] kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.7 2.7.11.7]~~ </~~span~~></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5dyj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5dyj OCA], [~~http~~://pdbe.org/5dyj PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5dyj RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5dyj PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5dyj ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/MHCKA_DICDI MHCKA_DICDI]] Phosphorylates threonine in the C-terminal tail region of myosin II heavy chain. This phosphorylation is critical in regulating the assembly and disassembly of myosin II filament. Requires autophosphorylation for activity.	+	[https://www.uniprot.org/uniprot/MHCKA_DICDI MHCKA_DICDI] Phosphorylates threonine in the C-terminal tail region of myosin II heavy chain. This phosphorylation is critical in regulating the assembly and disassembly of myosin II filament. Requires autophosphorylation for activity.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 25:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Dicdi~~]]	+	[[Category: Dictyostelium discoideum]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Jia, Z]]	+	[[Category: Jia Z]]
-	~~[[Category: Staalduinen, L M.van~~]]	+	[[Category: Yang Y]]
-	[[Category: Yang, Y]]	+	[[Category: Van Staalduinen LM]]
-	[[Category: ~~Kinase]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 08:13, 15 April 2020

OCA — Wed, 15 Apr 2020 08:13:35 GMT

←Older revision		Revision as of 08:13, 15 April 2020
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	==Mysosin heavy chain kinase A catalytic domain mutant - D663A==		==Mysosin heavy chain kinase A catalytic domain mutant - D663A==
-	<StructureSection load='5dyj' size='340' side='right' caption='[[5dyj]], [[Resolution\|resolution]] 2.50Å' scene=''>	+	<StructureSection load='5dyj' size='340' side='right'caption='[[5dyj]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5dyj]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5DYJ OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5DYJ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5dyj]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Dicdi Dicdi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5DYJ OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5DYJ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=TPO:PHOSPHOTHREONINE'>TPO</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=TPO:PHOSPHOTHREONINE'>TPO</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">mhkA, mhckA, DDB_G0291231 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=44689 DICDI])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/[Myosin_heavy-chain]_kinase [Myosin heavy-chain] kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.7 2.7.11.7] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/[Myosin_heavy-chain]_kinase [Myosin heavy-chain] kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.7 2.7.11.7] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5dyj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5dyj OCA], [http://pdbe.org/5dyj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5dyj RCSB], [http://www.ebi.ac.uk/pdbsum/5dyj PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5dyj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5dyj OCA], [http://pdbe.org/5dyj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5dyj RCSB], [http://www.ebi.ac.uk/pdbsum/5dyj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5dyj ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Dicdi]]
		+	[[Category: Large Structures]]
	[[Category: Jia, Z]]		[[Category: Jia, Z]]
	[[Category: Staalduinen, L M.van]]		[[Category: Staalduinen, L M.van]]

OCA at 15:21, 20 June 2016

OCA — Mon, 20 Jun 2016 15:21:31 GMT

←Older revision		Revision as of 15:21, 20 June 2016
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5dyj is ~~ON HOLD until Paper~~ Publication	+	==Mysosin heavy chain kinase A catalytic domain mutant - D663A==
		+	<StructureSection load='5dyj' size='340' side='right' caption='[[5dyj]], [[Resolution\|resolution]] 2.50Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5dyj]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5DYJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5DYJ FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=TPO:PHOSPHOTHREONINE'>TPO</scene></td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/[Myosin_heavy-chain]_kinase [Myosin heavy-chain] kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.7 2.7.11.7] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5dyj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5dyj OCA], [http://pdbe.org/5dyj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5dyj RCSB], [http://www.ebi.ac.uk/pdbsum/5dyj PDBsum]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/MHCKA_DICDI MHCKA_DICDI]] Phosphorylates threonine in the C-terminal tail region of myosin II heavy chain. This phosphorylation is critical in regulating the assembly and disassembly of myosin II filament. Requires autophosphorylation for activity.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The alpha-kinases are a family of a typical protein kinases present in organisms ranging from protozoa to mammals. Here we report an autoinhibited conformation for the alpha-kinase domain of Dictyostelium myosin-II heavy chain kinase A (MHCK-A) in which nucleotide binding to the catalytic cleft, located at the interface between an N-terminal and C-terminal lobe, is sterically blocked by the side chain of a conserved arginine residue (Arg592). Previous alpha-kinase structures have shown that an invariant catalytic aspartic acid residue (Asp766) is phosphorylated. Unexpectedly, in the autoinhibited conformation the phosphoryl group is transferred to the adjacent Asp663, creating an interaction network that stabilizes the autoinhibited state. The results suggest that Asp766 phosphorylation may play both catalytic and regulatory roles. The autoinhibited structure also provides the first view of a phosphothreonine residue docked into the phospho-specific allosteric binding site (Pi-pocket) in the C-lobe of the alpha-kinase domain.

-	~~Authors:~~ van Staalduinen, L~~.M.~~, ~~Yang~~, Y., Jia, Z.	+	Structure of the Dictyostelium Myosin-II Heavy Chain Kinase A (MHCK-A) alpha-kinase domain apoenzyme reveals a novel autoinhibited conformation.,Ye Q, Yang Y, van Staalduinen L, Crawley SW, Liu L, Brennan S, Cote GP, Jia Z Sci Rep. 2016 May 23;6:26634. doi: 10.1038/srep26634. PMID:27211275<ref>PMID:27211275</ref>

-	~~Description: Mysosin heavy chain kinase A catalytic domain mutant -D663A~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category~~: ~~Unreleased Structures]]~~	+	</div>
		+	<div class="pdbe-citations 5dyj" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Jia, Z]]		[[Category: Jia, Z]]
-	[[Category: ~~Van~~ Staalduinen, L.M]]	+	[[Category: Staalduinen, L M.van]]
	[[Category: Yang, Y]]		[[Category: Yang, Y]]
		+	[[Category: Kinase]]
		+	[[Category: Transferase]]

OCA at 22:02, 10 May 2016

OCA — Tue, 10 May 2016 22:02:07 GMT

←Older revision		Revision as of 22:02, 10 May 2016
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5dyj is ON HOLD	+	The entry 5dyj is ON HOLD until Paper Publication

	Authors: van Staalduinen, L.M., Yang, Y., Jia, Z.		Authors: van Staalduinen, L.M., Yang, Y., Jia, Z.

OCA at 11:31, 16 December 2015

OCA — Wed, 16 Dec 2015 11:31:09 GMT

←Older revision		Revision as of 11:31, 16 December 2015
Line 8:		Line 8:
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: Jia, Z]]		[[Category: Jia, Z]]
-	[[Category: Yang, Y]]
	[[Category: Van Staalduinen, L.M]]		[[Category: Van Staalduinen, L.M]]
		+	[[Category: Yang, Y]]

OCA at 12:42, 7 October 2015

OCA — Wed, 07 Oct 2015 12:42:44 GMT

←Older revision		Revision as of 12:42, 7 October 2015
Line 3:		Line 3:
	The entry 5dyj is ON HOLD		The entry 5dyj is ON HOLD

-	Authors: van Staalduinen, L,M, Yang, Y, Jia, Z	+	Authors: van Staalduinen, L.M., Yang, Y., Jia, Z.

	Description: Mysosin heavy chain kinase A catalytic domain mutant -D663A		Description: Mysosin heavy chain kinase A catalytic domain mutant -D663A
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Van Staalduinen, L,M~~, Yang, Y, Jia, Z~~]]	+	[[Category: Jia, Z]]
		+	[[Category: Yang, Y]]
		+	[[Category: Van Staalduinen, L.M]]

OCA: Protected "5dyj" [edit=sysop:move=sysop]

OCA — Wed, 30 Sep 2015 13:42:37 GMT

Protected "5dyj" [edit=sysop:move=sysop]

←Older revision

Revision as of 13:42, 30 September 2015

OCA: New page: '''Unreleased structure''' The entry 5dyj is ON HOLD Authors: van Staalduinen, L,M, Yang, Y, Jia, Z Description: Mysosin heavy chain kinase A catalytic domain mutant -D663A [[Category:...

OCA — Wed, 30 Sep 2015 13:42:36 GMT

New page: '''Unreleased structure''' The entry 5dyj is ON HOLD Authors: van Staalduinen, L,M, Yang, Y, Jia, Z Description: Mysosin heavy chain kinase A catalytic domain mutant -D663A [[Category:...

New page

'''Unreleased structure'''

The entry 5dyj is ON HOLD

Authors: van Staalduinen, L,M, Yang, Y, Jia, Z

Description: Mysosin heavy chain kinase A catalytic domain mutant -D663A
[[Category: Unreleased Structures]]
[[Category: Van Staalduinen, L,M, Yang, Y, Jia, Z]]