5e96 - Revision history

OCA at 06:17, 5 July 2023

OCA — Wed, 05 Jul 2023 06:17:46 GMT

←Older revision		Revision as of 06:17, 5 July 2023
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	<StructureSection load='5e96' size='340' side='right'caption='[[5e96]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='5e96' size='340' side='right'caption='[[5e96]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5e96]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Atcc_19434 Atcc 19434~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5E96 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5E96 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5e96]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Enterococcus_faecium Enterococcus faecium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5E96 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5E96 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat">~~<scene name='pdbligand=EDO:1~~,2~~-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.1Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat">~~[[1n71\|1n71]]~~, ~~[[1b87\|1b87]]</td~~></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
-	<~~tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b~~></td>~~<td class="sblockDat">aac(6 )-II ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1352 ATCC 19434])~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5e96 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5e96 OCA], [https://pdbe.org/5e96 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5e96 RCSB], [https://www.ebi.ac.uk/pdbsum/5e96 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5e96 ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5e96 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5e96 OCA], [~~http~~://pdbe.org/5e96 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5e96 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5e96 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5e96 ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q47764_ENTFC Q47764_ENTFC]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Atcc 19434~~]]	+	[[Category: Enterococcus faecium]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Baettig~~, O M~~]]	+	[[Category: Baettig OM]]
-	[[Category: Berghuis~~, A M~~]]	+	[[Category: Berghuis AM]]
-	[[Category: Burk~~, D L~~]]	+	[[Category: Burk DL]]
-	[[Category: Shi, K]]	+	[[Category: Shi K]]
-	~~[[Category: Acetyltransferase]]~~	+
-	~~[[Category: Aminoglycoside resistance]]~~	+
-	~~[[Category: Gnat]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 08:02, 8 January 2020

OCA — Wed, 08 Jan 2020 08:02:06 GMT

←Older revision		Revision as of 08:02, 8 January 2020
Line 1:		Line 1:

	==Crystal structure of aminoglycoside 6'-acetyltransferase type Ii==		==Crystal structure of aminoglycoside 6'-acetyltransferase type Ii==
-	<StructureSection load='5e96' size='340' side='right' caption='[[5e96]], [[Resolution\|resolution]] 2.10Å' scene=''>	+	<StructureSection load='5e96' size='340' side='right'caption='[[5e96]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5e96]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5E96 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5E96 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5e96]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_19434 Atcc 19434]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5E96 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5E96 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1n71\|1n71]], [[1b87\|1b87]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1n71\|1n71]], [[1b87\|1b87]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">aac(6 )-II ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1352 ATCC 19434])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5e96 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5e96 OCA], [http://pdbe.org/5e96 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5e96 RCSB], [http://www.ebi.ac.uk/pdbsum/5e96 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5e96 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5e96 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5e96 OCA], [http://pdbe.org/5e96 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5e96 RCSB], [http://www.ebi.ac.uk/pdbsum/5e96 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5e96 ProSAT]</span></td></tr>
	</table>		</table>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Atcc 19434]]
		+	[[Category: Large Structures]]
	[[Category: Baettig, O M]]		[[Category: Baettig, O M]]
	[[Category: Berghuis, A M]]		[[Category: Berghuis, A M]]

OCA at 02:13, 13 July 2016

OCA — Wed, 13 Jul 2016 02:13:50 GMT

←Older revision		Revision as of 02:13, 13 July 2016
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5e96 is ~~ON HOLD until Paper~~ Publication	+	==Crystal structure of aminoglycoside 6'-acetyltransferase type Ii==
		+	<StructureSection load='5e96' size='340' side='right' caption='[[5e96]], [[Resolution\|resolution]] 2.10Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5e96]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5E96 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5E96 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1n71\|1n71]], [[1b87\|1b87]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5e96 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5e96 OCA], [http://pdbe.org/5e96 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5e96 RCSB], [http://www.ebi.ac.uk/pdbsum/5e96 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5e96 ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	An enzyme's inherent structural plasticity is frequently associated with substrate binding, yet detailed structural characterization of flexible proteins remains challenging. This study employs complementary biophysical methods to characterize the partially unfolded structure of substrate-free AAC(6')-Ii, an N-acetyltransferase of the GCN5-related N-acetyltransferase (GNAT) superfamily implicated in conferring broad-spectrum aminoglycoside resistance on Enterococcus faecium. The X-ray crystal structure of AAC(6')-Ii is analyzed to identify relative motions of the structural elements that constitute the dimeric enzyme. Comparison with the previously elucidated crystal structure of AAC(6')-Ii with acetyl coenzyme A (AcCoA) reveals conformational changes that occur upon substrate binding. Our understanding of the enzyme's structural plasticity is further refined with small-angle X-ray scattering and circular dichroism analyses, which together reveal how flexible structural elements impact dimerization and substrate binding. These results clarify the extent of unfolding that AAC(6')-Ii undergoes in the absence of AcCoA and provide a structural connection to previously observed allosteric cooperativity of this enzyme. DATABASE: Structural data are available in the PDB database under the accession number 5E96.

-	~~Authors: Berghuis, A.M~~., Burk, D.L.~~, Baettig, O~~.M.~~, Shi, K~~.	+	Comprehensive characterization of ligand-induced plasticity changes in a dimeric enzyme.,Baettig OM, Shi K, Yachnin BJ, Burk DL, Berghuis AM FEBS J. 2016 Jun 22. doi: 10.1111/febs.13788. PMID:27333541<ref>PMID:27333541</ref>

-	~~Description: Crystal structure~~ of ~~aminoglycoside 6'~~-~~acetyltransferase type Ii~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
-	[[Category: ~~Baettig~~, O.M]]	+	<div class="pdbe-citations 5e96" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Baettig, O M]]
		+	[[Category: Berghuis, A M]]
		+	[[Category: Burk, D L]]
	[[Category: Shi, K]]		[[Category: Shi, K]]
-	[[Category: ~~Berghuis, A.M~~]]	+	[[Category: Acetyltransferase]]
-	[[Category: ~~Burk, D.L~~]]	+	[[Category: Aminoglycoside resistance]]
		+	[[Category: Gnat]]
		+	[[Category: Transferase]]

OCA at 02:32, 1 December 2015

OCA — Tue, 01 Dec 2015 02:32:14 GMT

←Older revision		Revision as of 02:32, 1 December 2015
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5e96 is ON HOLD	+	The entry 5e96 is ON HOLD until Paper Publication

	Authors: Berghuis, A.M., Burk, D.L., Baettig, O.M., Shi, K.		Authors: Berghuis, A.M., Burk, D.L., Baettig, O.M., Shi, K.

OCA: Protected "5e96" [edit=sysop:move=sysop]

OCA — Thu, 22 Oct 2015 04:32:50 GMT

Protected "5e96" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:32, 22 October 2015

OCA: New page: '''Unreleased structure''' The entry 5e96 is ON HOLD Authors: Berghuis, A.M., Burk, D.L., Baettig, O.M., Shi, K. Description: Crystal structure of aminoglycoside 6'-acetyltransferase t...

OCA — Thu, 22 Oct 2015 04:32:49 GMT

New page: '''Unreleased structure''' The entry 5e96 is ON HOLD Authors: Berghuis, A.M., Burk, D.L., Baettig, O.M., Shi, K. Description: Crystal structure of aminoglycoside 6'-acetyltransferase t...

New page

'''Unreleased structure'''

The entry 5e96 is ON HOLD

Authors: Berghuis, A.M., Burk, D.L., Baettig, O.M., Shi, K.

Description: Crystal structure of aminoglycoside 6'-acetyltransferase type Ii
[[Category: Unreleased Structures]]
[[Category: Baettig, O.M]]
[[Category: Shi, K]]
[[Category: Berghuis, A.M]]
[[Category: Burk, D.L]]