5efd - Revision history

OCA at 09:10, 20 March 2024

OCA — Wed, 20 Mar 2024 09:10:51 GMT

←Older revision		Revision as of 09:10, 20 March 2024
Line 10:		Line 10:
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/O30700_9BACI O30700_9BACI]		[https://www.uniprot.org/uniprot/O30700_9BACI O30700_9BACI]
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Cryptic pockets are visible in ligand-bound protein structures but are occluded in unbound structures. Utilizing these pockets in fragment-based drug-design provides an attractive option for proteins not tractable by classical binding sites. However, owing to their hidden nature, they are difficult to identify. Here, we show that small glycols find cryptic pockets on a diverse set of proteins. Initial crystallography experiments serendipitously revealed the ability of ethylene glycol, a small glycol, to identify a cryptic pocket on the W6A mutant of the RBSX protein (RBSX-W6A). Explicit-solvent molecular dynamics (MD) simulations of RBSX-W6A with the exposed state of the cryptic pocket (ethylene glycol removed) revealed closure of the pocket reiterating that the exposed state of cryptic pockets in general are unstable in the absence of ligands. Also, no change in the pocket was observed for simulations of RBSX-W6A with the occluded state of the cryptic pocket, suggesting that water molecules are not able to open the cryptic pocket. "Cryptic-pocket finding" potential of small glycols was then supported and generalized through additional crystallography experiments, explicit-cosolvent MD simulations, and protein data set construction and analysis. The cryptic pocket on RBSX-W6A was found again upon repeating the crystallography experiments with another small glycol, propylene glycol. Use of ethylene glycol as a probe molecule in cosolvent MD simulations led to the enhanced sampling of the exposed state of experimentally observed cryptic sites on a test set of two proteins (Niemann-Pick C2, Interleukin-2). Further, analyses of protein structures with validated cryptic sites showed that ethylene glycol molecules bind to sites on proteins (Bcl-xL, G-actin, myosin II, and glutamate receptor 2), which become apparent upon binding of biologically relevant ligands. Our study thus suggests potential application of the small glycols in experimental and computational fragment-based approaches to identify cryptic pockets in apparently undruggable and/or difficult targets, making these proteins amenable to drug-design strategies.
-
-	Small Glycols Discover Cryptic Pockets on Proteins for Fragment-Based Approaches.,Bansia H, Mahanta P, Yennawar NH, Ramakumar S J Chem Inf Model. 2021 Mar 22;61(3):1322-1333. doi: 10.1021/acs.jcim.0c01126., Epub 2021 Feb 11. PMID:33570386<ref>PMID:33570386</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 5efd" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 06:24, 5 July 2023

OCA — Wed, 05 Jul 2023 06:24:12 GMT

←Older revision		Revision as of 06:24, 5 July 2023
Line 3:		Line 3:
	<StructureSection load='5efd' size='340' side='right'caption='[[5efd]], [[Resolution\|resolution]] 1.67Å' scene=''>		<StructureSection load='5efd' size='340' side='right'caption='[[5efd]], [[Resolution\|resolution]] 1.67Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5efd]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_sp.~~_ng~~-27 Bacillus sp. ng-27]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EFD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5EFD FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5efd]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_sp._NG-27 Bacillus sp. NG-27]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EFD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5EFD FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene~~></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.674Å</td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[5eb8\|5eb8]], [[5eba\|5eba]], [[5ebf\|5ebf]]</div></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Endo-1,4-beta-xylanase Endo-1,4-beta-xylanase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.8 3.2.1.8] </span></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5efd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5efd OCA], [https://pdbe.org/5efd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5efd RCSB], [https://www.ebi.ac.uk/pdbsum/5efd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5efd ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5efd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5efd OCA], [https://pdbe.org/5efd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5efd RCSB], [https://www.ebi.ac.uk/pdbsum/5efd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5efd ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/O30700_9BACI O30700_9BACI]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 22:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Bacillus sp. ng-27]]	+	[[Category: Bacillus sp. NG-27]]
-	~~[[Category: Endo-1,4-beta-xylanase~~]]	+
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Bhardwaj, A]]	+	[[Category: Bhardwaj A]]
-	[[Category: Mahanta, P]]	+	[[Category: Mahanta P]]
-	[[Category: Ramakumar, S]]	+	[[Category: Ramakumar S]]
-	[[Category: Reddy~~, V S]]~~	+	[[Category: Reddy VS]]
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Mutant]]~~	+
-	~~[[Category: Xylanase~~]]	+

OCA at 06:44, 24 March 2021

OCA — Wed, 24 Mar 2021 06:44:33 GMT

←Older revision		Revision as of 06:44, 24 March 2021
Line 3:		Line 3:
	<StructureSection load='5efd' size='340' side='right'caption='[[5efd]], [[Resolution\|resolution]] 1.67Å' scene=''>		<StructureSection load='5efd' size='340' side='right'caption='[[5efd]], [[Resolution\|resolution]] 1.67Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5efd]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Bacillus_sp._ng-27 Bacillus sp. ng-27]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EFD OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5EFD FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5efd]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_sp._ng-27 Bacillus sp. ng-27]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EFD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5EFD FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5eb8\|5eb8]], [[5eba\|5eba]], [[5ebf\|5ebf]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[5eb8\|5eb8]], [[5eba\|5eba]], [[5ebf\|5ebf]]</div></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Endo-1,4-beta-xylanase Endo-1,4-beta-xylanase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.8 3.2.1.8] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Endo-1,4-beta-xylanase Endo-1,4-beta-xylanase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.8 3.2.1.8] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5efd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5efd OCA], [~~http~~://pdbe.org/5efd PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5efd RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5efd PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5efd ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5efd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5efd OCA], [https://pdbe.org/5efd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5efd RCSB], [https://www.ebi.ac.uk/pdbsum/5efd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5efd ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Cryptic pockets are visible in ligand-bound protein structures but are occluded in unbound structures. Utilizing these pockets in fragment-based drug-design provides an attractive option for proteins not tractable by classical binding sites. However, owing to their hidden nature, they are difficult to identify. Here, we show that small glycols find cryptic pockets on a diverse set of proteins. Initial crystallography experiments serendipitously revealed the ability of ethylene glycol, a small glycol, to identify a cryptic pocket on the W6A mutant of the RBSX protein (RBSX-W6A). Explicit-solvent molecular dynamics (MD) simulations of RBSX-W6A with the exposed state of the cryptic pocket (ethylene glycol removed) revealed closure of the pocket reiterating that the exposed state of cryptic pockets in general are unstable in the absence of ligands. Also, no change in the pocket was observed for simulations of RBSX-W6A with the occluded state of the cryptic pocket, suggesting that water molecules are not able to open the cryptic pocket. "Cryptic-pocket finding" potential of small glycols was then supported and generalized through additional crystallography experiments, explicit-cosolvent MD simulations, and protein data set construction and analysis. The cryptic pocket on RBSX-W6A was found again upon repeating the crystallography experiments with another small glycol, propylene glycol. Use of ethylene glycol as a probe molecule in cosolvent MD simulations led to the enhanced sampling of the exposed state of experimentally observed cryptic sites on a test set of two proteins (Niemann-Pick C2, Interleukin-2). Further, analyses of protein structures with validated cryptic sites showed that ethylene glycol molecules bind to sites on proteins (Bcl-xL, G-actin, myosin II, and glutamate receptor 2), which become apparent upon binding of biologically relevant ligands. Our study thus suggests potential application of the small glycols in experimental and computational fragment-based approaches to identify cryptic pockets in apparently undruggable and/or difficult targets, making these proteins amenable to drug-design strategies.
		+
		+	Small Glycols Discover Cryptic Pockets on Proteins for Fragment-Based Approaches.,Bansia H, Mahanta P, Yennawar NH, Ramakumar S J Chem Inf Model. 2021 Mar 22;61(3):1322-1333. doi: 10.1021/acs.jcim.0c01126., Epub 2021 Feb 11. PMID:33570386<ref>PMID:33570386</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5efd" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 06:56, 22 April 2020

OCA — Wed, 22 Apr 2020 06:56:18 GMT

←Older revision		Revision as of 06:56, 22 April 2020
Line 1:		Line 1:

	==Crystal structure of a surface pocket creating mutant (W6A) of an alkali thermostable GH10 xylanase from Bacillus sp. NG-27==		==Crystal structure of a surface pocket creating mutant (W6A) of an alkali thermostable GH10 xylanase from Bacillus sp. NG-27==
-	<StructureSection load='5efd' size='340' side='right' caption='[[5efd]], [[Resolution\|resolution]] 1.67Å' scene=''>	+	<StructureSection load='5efd' size='340' side='right'caption='[[5efd]], [[Resolution\|resolution]] 1.67Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5efd]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EFD OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5EFD FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5efd]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_sp._ng-27 Bacillus sp. ng-27]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EFD OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5EFD FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5eb8\|5eb8]], [[5eba\|5eba]], [[5ebf\|5ebf]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5eb8\|5eb8]], [[5eba\|5eba]], [[5ebf\|5ebf]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Endo-1,4-beta-xylanase Endo-1,4-beta-xylanase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.8 3.2.1.8] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Endo-1,4-beta-xylanase Endo-1,4-beta-xylanase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.8 3.2.1.8] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5efd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5efd OCA], [http://pdbe.org/5efd PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5efd RCSB], [http://www.ebi.ac.uk/pdbsum/5efd PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5efd ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5efd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5efd OCA], [http://pdbe.org/5efd PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5efd RCSB], [http://www.ebi.ac.uk/pdbsum/5efd PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5efd ProSAT]</span></td></tr>
	</table>		</table>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Bacillus sp. ng-27]]
	[[Category: Endo-1,4-beta-xylanase]]		[[Category: Endo-1,4-beta-xylanase]]
		+	[[Category: Large Structures]]
	[[Category: Bhardwaj, A]]		[[Category: Bhardwaj, A]]
	[[Category: Mahanta, P]]		[[Category: Mahanta, P]]

OCA at 18:39, 26 October 2016

OCA — Wed, 26 Oct 2016 18:39:43 GMT

←Older revision		Revision as of 18:39, 26 October 2016
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry 5efd is ON HOLD until Paper Publication~~	+	==Crystal structure of a surface pocket creating mutant (W6A) of an alkali thermostable GH10 xylanase from Bacillus sp. NG-27==
-		+	<StructureSection load='5efd' size='340' side='right' caption='[[5efd]], [[Resolution\|resolution]] 1.67Å' scene=''>
-	~~Authors: Mahanta, P., Bhardwaj, A., Reddy, V.S., Ramakumar, S.~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5efd]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EFD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5EFD FirstGlance]. <br>
-	~~Description:~~ Crystal structure of a surface pocket creating mutant (W6A) of an alkali thermostable GH10 xylanase from Bacillus sp. NG-27	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5eb8\|5eb8]], [[5eba\|5eba]], [[5ebf\|5ebf]]</td></tr>
-	[[~~Category~~: ~~Reddy~~, V.S]]	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Endo-1,4-beta-xylanase Endo-1,4-beta-xylanase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.8 3.2.1.8] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5efd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5efd OCA], [http://pdbe.org/5efd PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5efd RCSB], [http://www.ebi.ac.uk/pdbsum/5efd PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5efd ProSAT]</span></td></tr>
		+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Endo-1,4-beta-xylanase]]
	[[Category: Bhardwaj, A]]		[[Category: Bhardwaj, A]]
	[[Category: Mahanta, P]]		[[Category: Mahanta, P]]
	[[Category: Ramakumar, S]]		[[Category: Ramakumar, S]]
		+	[[Category: Reddy, V S]]
		+	[[Category: Hydrolase]]
		+	[[Category: Mutant]]
		+	[[Category: Xylanase]]

OCA at 10:41, 1 June 2016

OCA — Wed, 01 Jun 2016 10:41:02 GMT

←Older revision		Revision as of 10:41, 1 June 2016
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5efd is ON HOLD	+	The entry 5efd is ON HOLD until Paper Publication

	Authors: Mahanta, P., Bhardwaj, A., Reddy, V.S., Ramakumar, S.		Authors: Mahanta, P., Bhardwaj, A., Reddy, V.S., Ramakumar, S.

OCA: Protected "5efd" [edit=sysop:move=sysop]

OCA — Tue, 01 Dec 2015 02:45:31 GMT

Protected "5efd" [edit=sysop:move=sysop]

←Older revision

Revision as of 02:45, 1 December 2015

OCA: New page: '''Unreleased structure''' The entry 5efd is ON HOLD Authors: Mahanta, P., Bhardwaj, A., Reddy, V.S., Ramakumar, S. Description: Crystal structure of a surface pocket creating mutant (...

OCA — Tue, 01 Dec 2015 02:45:29 GMT

New page: '''Unreleased structure''' The entry 5efd is ON HOLD Authors: Mahanta, P., Bhardwaj, A., Reddy, V.S., Ramakumar, S. Description: Crystal structure of a surface pocket creating mutant (...

New page

'''Unreleased structure'''

The entry 5efd is ON HOLD

Authors: Mahanta, P., Bhardwaj, A., Reddy, V.S., Ramakumar, S.

Description: Crystal structure of a surface pocket creating mutant (W6A) of an alkali thermostable GH10 xylanase from Bacillus sp. NG-27
[[Category: Unreleased Structures]]
[[Category: Reddy, V.S]]
[[Category: Bhardwaj, A]]
[[Category: Mahanta, P]]
[[Category: Ramakumar, S]]