5eib - Revision history

OCA at 06:29, 5 July 2023

OCA — Wed, 05 Jul 2023 06:29:17 GMT

←Older revision		Revision as of 06:29, 5 July 2023
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	<StructureSection load='5eib' size='340' side='right'caption='[[5eib]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='5eib' size='340' side='right'caption='[[5eib]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5eib]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [~~http~~://en.wikipedia.org/wiki/~~Synthetic_construct_sequences~~ Synthetic construct ~~sequences~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EIB OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5EIB FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5eib]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus], [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EIB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5EIB FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.1Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5eib FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5eib OCA], [~~http~~://pdbe.org/5eib PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5eib RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5eib PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5eib ProSAT]</span></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5eib FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5eib OCA], [https://pdbe.org/5eib PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5eib RCSB], [https://www.ebi.ac.uk/pdbsum/5eib PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5eib ProSAT]</span></td></tr>
	</table>		</table>
-	== Disease ==
-	[[http://www.uniprot.org/uniprot/CENPJ_HUMAN CENPJ_HUMAN]] Seckel syndrome;Autosomal recessive primary microcephaly. The disease is caused by mutations affecting the gene represented in this entry. The disease is caused by mutations affecting the gene represented in this entry.
	== Function ==		== Function ==
-	[[http://www.uniprot.org/uniprot/CENPJ_HUMAN CENPJ_HUMAN]] Plays an important role in cell division and centrosome function by participating in centriole duplication. Inhibits microtubule nucleation from the centrosome.<ref>PMID:15047868</ref> <ref>PMID:17681131</ref> <ref>PMID:20531387</ref> [[http://www.uniprot.org/uniprot/TBA1B_BOVIN TBA1B_BOVIN]] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. [[http://www.uniprot.org/uniprot/TBB2B_BOVIN TBB2B_BOVIN]] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain ~~(By similarity)~~.	+	[https://www.uniprot.org/uniprot/TBA1B_BOVIN TBA1B_BOVIN] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</StructureSection>		</StructureSection>
	[[Category: Bos taurus]]		[[Category: Bos taurus]]
		+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Synthetic construct ~~sequences~~]]	+	[[Category: Synthetic construct]]
-	[[Category: Li, H]]	+	[[Category: Li H]]
-	[[Category: Zheng, X]]	+	[[Category: Zheng X]]
-	~~[[Category: Cell cycle]]~~	+
-	~~[[Category: Pn2-3]]~~	+
-	~~[[Category: Tubulin complex~~]]	+

OCA at 06:59, 22 April 2020

OCA — Wed, 22 Apr 2020 06:59:23 GMT

←Older revision		Revision as of 06:59, 22 April 2020
Line 1:		Line 1:

	==Crystal structure of CPAP PN2-3 C-terminal loop-helix in complex with DARPin-tubulin==		==Crystal structure of CPAP PN2-3 C-terminal loop-helix in complex with DARPin-tubulin==
-	<StructureSection load='5eib' size='340' side='right' caption='[[5eib]], [[Resolution\|resolution]] 2.10Å' scene=''>	+	<StructureSection load='5eib' size='340' side='right'caption='[[5eib]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5eib]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/ ] and [http://en.wikipedia.org/wiki/~~Bos_taurus Bos taurus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EIB OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5EIB FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5eib]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [http://en.wikipedia.org/wiki/Synthetic_construct_sequences Synthetic construct sequences]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EIB OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5EIB FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5eib FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5eib OCA], [http://pdbe.org/5eib PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5eib RCSB], [http://www.ebi.ac.uk/pdbsum/5eib PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5eib ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5eib FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5eib OCA], [http://pdbe.org/5eib PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5eib RCSB], [http://www.ebi.ac.uk/pdbsum/5eib PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5eib ProSAT]</span></td></tr>
	</table>		</table>
	== Disease ==		== Disease ==
Line 20:		Line 20:
	</div>		</div>
	<div class="pdbe-citations 5eib" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5eib" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Tubulin 3D Structures\|Tubulin 3D Structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 25:		Line 28:
	</StructureSection>		</StructureSection>
	[[Category: Bos taurus]]		[[Category: Bos taurus]]
		+	[[Category: Large Structures]]
		+	[[Category: Synthetic construct sequences]]
	[[Category: Li, H]]		[[Category: Li, H]]
	[[Category: Zheng, X]]		[[Category: Zheng, X]]

OCA at 18:30, 26 October 2016

OCA — Wed, 26 Oct 2016 18:30:18 GMT

←Older revision		Revision as of 18:30, 26 October 2016
Line 1:		Line 1:
-	'''Unreleased structure'''

-	The entry ~~5eib~~ is ~~ON HOLD~~ ~~until Paper~~ Publication	+	==Crystal structure of CPAP PN2-3 C-terminal loop-helix in complex with DARPin-tubulin==
		+	<StructureSection load='5eib' size='340' side='right' caption='[[5eib]], [[Resolution\|resolution]] 2.10Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5eib]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/ ] and [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EIB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5EIB FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5eib FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5eib OCA], [http://pdbe.org/5eib PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5eib RCSB], [http://www.ebi.ac.uk/pdbsum/5eib PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5eib ProSAT]</span></td></tr>
		+	</table>
		+	== Disease ==
		+	[[http://www.uniprot.org/uniprot/CENPJ_HUMAN CENPJ_HUMAN]] Seckel syndrome;Autosomal recessive primary microcephaly. The disease is caused by mutations affecting the gene represented in this entry. The disease is caused by mutations affecting the gene represented in this entry.
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/CENPJ_HUMAN CENPJ_HUMAN]] Plays an important role in cell division and centrosome function by participating in centriole duplication. Inhibits microtubule nucleation from the centrosome.<ref>PMID:15047868</ref> <ref>PMID:17681131</ref> <ref>PMID:20531387</ref> [[http://www.uniprot.org/uniprot/TBA1B_BOVIN TBA1B_BOVIN]] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. [[http://www.uniprot.org/uniprot/TBB2B_BOVIN TBB2B_BOVIN]] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain (By similarity).
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Centrioles and cilia are microtubule-based structures, whose precise formation requires controlled cytoplasmic tubulin incorporation. How cytoplasmic tubulin is recognized for centriolar/ciliary-microtubule construction remains poorly understood. Centrosomal-P4.1-associated-protein (CPAP) binds tubulin via its PN2-3 domain. Here, we show that a C-terminal loop-helix in PN2-3 targets beta-tubulin at the microtubule outer surface, while an N-terminal helical motif caps microtubule's alpha-beta surface of beta-tubulin. Through this, PN2-3 forms a high-affinity complex with GTP-tubulin, crucial for defining numbers and lengths of centriolar/ciliary-microtubules. Surprisingly, two distinct mutations in PN2-3 exhibit opposite effects on centriolar/ciliary-microtubule lengths. CPAP(F375A), with strongly reduced tubulin interaction, causes shorter centrioles and cilia exhibiting doublet- instead of triplet-microtubules. CPAP(EE343RR) that unmasks the beta-tubulin polymerization surface displays slightly reduced tubulin-binding affinity inducing over-elongation of newly forming centriolar/ciliary-microtubules by enhanced dynamic release of its bound tubulin. Thus CPAP regulates delivery of its bound-tubulin to define the size of microtubule-based cellular structures using a 'clutch-like' mechanism.

-	~~Authors:~~ Li, H., ~~Zheng~~, X.	+	Molecular basis for CPAP-tubulin interaction in controlling centriolar and ciliary length.,Zheng X, Ramani A, Soni K, Gottardo M, Zheng S, Ming Gooi L, Li W, Feng S, Mariappan A, Wason A, Widlund P, Pozniakovsky A, Poser I, Deng H, Ou G, Riparbelli M, Giuliano C, Hyman AA, Sattler M, Gopalakrishnan J, Li H Nat Commun. 2016 Jun 16;7:11874. doi: 10.1038/ncomms11874. PMID:27306797<ref>PMID:27306797</ref>

-	~~Description: Crystal structure~~ of ~~CPAP PN2~~-~~3 C~~-~~terminal loop-helix in complex with DARPin-tubulin~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category~~: ~~Unreleased Structures]]~~	+	</div>
-	[[Category: ~~Zheng, X~~]]	+	<div class="pdbe-citations 5eib" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Bos taurus]]
	[[Category: Li, H]]		[[Category: Li, H]]
		+	[[Category: Zheng, X]]
		+	[[Category: Cell cycle]]
		+	[[Category: Pn2-3]]
		+	[[Category: Tubulin complex]]

OCA at 09:56, 7 January 2016

OCA — Thu, 07 Jan 2016 09:56:44 GMT

←Older revision		Revision as of 09:56, 7 January 2016
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5eib is ON HOLD	+	The entry 5eib is ON HOLD until Paper Publication

	Authors: Li, H., Zheng, X.		Authors: Li, H., Zheng, X.

OCA: Protected "5eib" [edit=sysop:move=sysop]

OCA — Tue, 01 Dec 2015 02:52:37 GMT

Protected "5eib" [edit=sysop:move=sysop]

←Older revision

Revision as of 02:52, 1 December 2015

OCA: New page: '''Unreleased structure''' The entry 5eib is ON HOLD Authors: Li, H., Zheng, X. Description: Crystal structure of CPAP PN2-3 C-terminal loop-helix in complex with DARPin-tubulin [[Cate...

OCA — Tue, 01 Dec 2015 02:52:36 GMT

New page: '''Unreleased structure''' The entry 5eib is ON HOLD Authors: Li, H., Zheng, X. Description: Crystal structure of CPAP PN2-3 C-terminal loop-helix in complex with DARPin-tubulin [[Cate...

New page

'''Unreleased structure'''

The entry 5eib is ON HOLD

Authors: Li, H., Zheng, X.

Description: Crystal structure of CPAP PN2-3 C-terminal loop-helix in complex with DARPin-tubulin
[[Category: Unreleased Structures]]
[[Category: Zheng, X]]
[[Category: Li, H]]