5ejj - Revision history

OCA at 09:11, 20 March 2024

OCA — Wed, 20 Mar 2024 09:11:04 GMT

←Older revision		Revision as of 09:11, 20 March 2024
Line 9:		Line 9:
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/UFSP_CAEEL UFSP_CAEEL] Thiol protease which recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ufm-1, a ubiquitin-like modifier protein bound to a number of target proteins (By similarity). Required, with oct-4, for the localization of a subset of 7 transmembrane domain odorant receptors, including odr-10, to the cilia of olfactory neurons AWA and AWC. Operates in aggregation behavior, and responses to oxygen levels.[UniProtKB:Q9NUQ7]<ref>PMID:12410303</ref> <ref>PMID:24603482</ref> <ref>PMID:9590179</ref>		[https://www.uniprot.org/uniprot/UFSP_CAEEL UFSP_CAEEL] Thiol protease which recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ufm-1, a ubiquitin-like modifier protein bound to a number of target proteins (By similarity). Required, with oct-4, for the localization of a subset of 7 transmembrane domain odorant receptors, including odr-10, to the cilia of olfactory neurons AWA and AWC. Operates in aggregation behavior, and responses to oxygen levels.[UniProtKB:Q9NUQ7]<ref>PMID:12410303</ref> <ref>PMID:24603482</ref> <ref>PMID:9590179</ref>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Ubiquitin-fold modifier 1 (Ufm1) specific protease (UfSP) is a novel cysteine protease that activates Ufm1 from its precursor by processing the C-terminus to expose the conserved Gly necessary for substrate conjugation and de-conjugates Ufm1 from the substrate. There are two forms: UfSP1 and UfSP2, the later with an additional domain at the N-terminus. Ufm1 and both the conjugating and deconjugating enzymes are highly conserved. However, in Caenorhabditis elegans there is one UfSP which has extra 136 residues at the N terminus compared to UfSP2. The crystal structure of cUfSP reveals that these additional residues display a MPN fold while the rest of the structure mimics that of UfSP2. The MPN domain does not have the metalloprotease activity found in some MPN-domain containing protein, rather it is required for the recognition and deufmylation of the substrate of cUfSP, UfBP1. In addition, the MPN domain is also required for localization to the endoplasmic reticulum.
-
-	The MPN domain of Caenorhabditis elegans UfSP modulates both substrate recognition and deufmylation activity.,Ha BH, Kim KH, Yoo HM, Lee W, EunKyeong Kim E Biochem Biophys Res Commun. 2016 Aug 5;476(4):450-6. doi:, 10.1016/j.bbrc.2016.05.143. Epub 2016 May 28. PMID:27240952<ref>PMID:27240952</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 5ejj" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 06:30, 5 July 2023

OCA — Wed, 05 Jul 2023 06:30:47 GMT

←Older revision		Revision as of 06:30, 5 July 2023
Line 3:		Line 3:
	<StructureSection load='5ejj' size='340' side='right'caption='[[5ejj]], [[Resolution\|resolution]] 2.80Å' scene=''>		<StructureSection load='5ejj' size='340' side='right'caption='[[5ejj]], [[Resolution\|resolution]] 2.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5ejj]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EJJ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5EJJ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5ejj]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Caenorhabditis_elegans Caenorhabditis elegans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EJJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5EJJ FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5ejj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ejj OCA], [~~http~~://pdbe.org/5ejj PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5ejj RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5ejj PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5ejj ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.8Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ejj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ejj OCA], [https://pdbe.org/5ejj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ejj RCSB], [https://www.ebi.ac.uk/pdbsum/5ejj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ejj ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/UFSP_CAEEL UFSP_CAEEL]] Thiol protease which recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ufm-1, a ubiquitin-like modifier protein bound to a number of target proteins (By similarity). Required, with oct-4, for the localization of a subset of 7 transmembrane domain odorant receptors, including odr-10, to the cilia of olfactory neurons AWA and AWC. Operates in aggregation behavior, and responses to oxygen levels.[UniProtKB:Q9NUQ7]<ref>PMID:12410303</ref> <ref>PMID:24603482</ref> <ref>PMID:9590179</ref>	+	[https://www.uniprot.org/uniprot/UFSP_CAEEL UFSP_CAEEL] Thiol protease which recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ufm-1, a ubiquitin-like modifier protein bound to a number of target proteins (By similarity). Required, with oct-4, for the localization of a subset of 7 transmembrane domain odorant receptors, including odr-10, to the cilia of olfactory neurons AWA and AWC. Operates in aggregation behavior, and responses to oxygen levels.[UniProtKB:Q9NUQ7]<ref>PMID:12410303</ref> <ref>PMID:24603482</ref> <ref>PMID:9590179</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 21:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Caenorhabditis elegans]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Ha, B]]	+	[[Category: Ha B]]
-	[[Category: Kim~~, E E~~]]	+	[[Category: Kim EE]]
-	[[Category: Kim, K]]	+	[[Category: Kim K]]
-	~~[[Category: Deufmylation]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Ufm1]]~~	+
-	~~[[Category: Ufsp~~]]	+

OCA at 07:01, 22 April 2020

OCA — Wed, 22 Apr 2020 07:01:26 GMT

←Older revision		Revision as of 07:01, 22 April 2020
Line 1:		Line 1:

	==Crystal structure of UfSP from C.elegans==		==Crystal structure of UfSP from C.elegans==
-	<StructureSection load='5ejj' size='340' side='right' caption='[[5ejj]], [[Resolution\|resolution]] 2.80Å' scene=''>	+	<StructureSection load='5ejj' size='340' side='right'caption='[[5ejj]], [[Resolution\|resolution]] 2.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5ejj]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EJJ OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5EJJ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5ejj]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EJJ OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5EJJ FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5ejj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ejj OCA], [http://pdbe.org/5ejj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ejj RCSB], [http://www.ebi.ac.uk/pdbsum/5ejj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ejj ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5ejj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ejj OCA], [http://pdbe.org/5ejj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ejj RCSB], [http://www.ebi.ac.uk/pdbsum/5ejj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ejj ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 21:		Line 21:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Ha, B]]		[[Category: Ha, B]]
	[[Category: Kim, E E]]		[[Category: Kim, E E]]

OCA at 10:32, 3 August 2017

OCA — Thu, 03 Aug 2017 10:32:03 GMT

←Older revision		Revision as of 10:32, 3 August 2017
Line 8:		Line 8:
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/UFSP_CAEEL UFSP_CAEEL]] Thiol protease which recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ufm-1, a ubiquitin-like modifier protein bound to a number of target proteins (By similarity). Required, with oct-4, for the localization of a subset of 7 transmembrane domain odorant receptors, including odr-10, to the cilia of olfactory neurons AWA and AWC. Operates in aggregation behavior, and responses to oxygen levels.[UniProtKB:Q9NUQ7]<ref>PMID:12410303</ref> <ref>PMID:24603482</ref> <ref>PMID:9590179</ref>		[[http://www.uniprot.org/uniprot/UFSP_CAEEL UFSP_CAEEL]] Thiol protease which recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ufm-1, a ubiquitin-like modifier protein bound to a number of target proteins (By similarity). Required, with oct-4, for the localization of a subset of 7 transmembrane domain odorant receptors, including odr-10, to the cilia of olfactory neurons AWA and AWC. Operates in aggregation behavior, and responses to oxygen levels.[UniProtKB:Q9NUQ7]<ref>PMID:12410303</ref> <ref>PMID:24603482</ref> <ref>PMID:9590179</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Ubiquitin-fold modifier 1 (Ufm1) specific protease (UfSP) is a novel cysteine protease that activates Ufm1 from its precursor by processing the C-terminus to expose the conserved Gly necessary for substrate conjugation and de-conjugates Ufm1 from the substrate. There are two forms: UfSP1 and UfSP2, the later with an additional domain at the N-terminus. Ufm1 and both the conjugating and deconjugating enzymes are highly conserved. However, in Caenorhabditis elegans there is one UfSP which has extra 136 residues at the N terminus compared to UfSP2. The crystal structure of cUfSP reveals that these additional residues display a MPN fold while the rest of the structure mimics that of UfSP2. The MPN domain does not have the metalloprotease activity found in some MPN-domain containing protein, rather it is required for the recognition and deufmylation of the substrate of cUfSP, UfBP1. In addition, the MPN domain is also required for localization to the endoplasmic reticulum.
		+
		+	The MPN domain of Caenorhabditis elegans UfSP modulates both substrate recognition and deufmylation activity.,Ha BH, Kim KH, Yoo HM, Lee W, EunKyeong Kim E Biochem Biophys Res Commun. 2016 Aug 5;476(4):450-6. doi:, 10.1016/j.bbrc.2016.05.143. Epub 2016 May 28. PMID:27240952<ref>PMID:27240952</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5ejj" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 00:37, 19 January 2017

OCA — Thu, 19 Jan 2017 00:37:15 GMT

←Older revision		Revision as of 00:37, 19 January 2017
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5ejj ~~is ON HOLD until Paper Publication~~	+	==Crystal structure of UfSP from C.elegans==
-		+	<StructureSection load='5ejj' size='340' side='right' caption='[[5ejj]], [[Resolution\|resolution]] 2.80Å' scene=''>
-	~~Authors~~: ~~Kim~~, K., Ha, B., ~~Kim~~, E.E.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5ejj]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EJJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5EJJ FirstGlance]. <br>
-	~~Description~~: ~~Crystal structure of UfSP from~~ C.~~elegans~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ejj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ejj OCA], [http://pdbe.org/5ejj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ejj RCSB], [http://www.ebi.ac.uk/pdbsum/5ejj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ejj ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
-	[[Category: Kim, E.E]]	+	== Function ==
		+	[[http://www.uniprot.org/uniprot/UFSP_CAEEL UFSP_CAEEL]] Thiol protease which recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ufm-1, a ubiquitin-like modifier protein bound to a number of target proteins (By similarity). Required, with oct-4, for the localization of a subset of 7 transmembrane domain odorant receptors, including odr-10, to the cilia of olfactory neurons AWA and AWC. Operates in aggregation behavior, and responses to oxygen levels.[UniProtKB:Q9NUQ7]<ref>PMID:12410303</ref> <ref>PMID:24603482</ref> <ref>PMID:9590179</ref>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Ha, B]]
		+	[[Category: Kim, E E]]
	[[Category: Kim, K]]		[[Category: Kim, K]]
-	[[Category: ~~Ha, B~~]]	+	[[Category: Deufmylation]]
		+	[[Category: Hydrolase]]
		+	[[Category: Ufm1]]
		+	[[Category: Ufsp]]

OCA: Protected "5ejj" [edit=sysop:move=sysop]

OCA — Tue, 01 Dec 2015 02:55:41 GMT

Protected "5ejj" [edit=sysop:move=sysop]

←Older revision

Revision as of 02:55, 1 December 2015

OCA: New page: '''Unreleased structure''' The entry 5ejj is ON HOLD until Paper Publication Authors: Kim, K., Ha, B., Kim, E.E. Description: Crystal structure of UfSP from C.elegans [[Category: Unrel...

OCA — Tue, 01 Dec 2015 02:55:39 GMT

New page: '''Unreleased structure''' The entry 5ejj is ON HOLD until Paper Publication Authors: Kim, K., Ha, B., Kim, E.E. Description: Crystal structure of UfSP from C.elegans [[Category: Unrel...

New page

'''Unreleased structure'''

The entry 5ejj is ON HOLD until Paper Publication

Authors: Kim, K., Ha, B., Kim, E.E.

Description: Crystal structure of UfSP from C.elegans
[[Category: Unreleased Structures]]
[[Category: Kim, E.E]]
[[Category: Kim, K]]
[[Category: Ha, B]]