5ejn - Revision history

OCA at 06:30, 5 July 2023

OCA — Wed, 05 Jul 2023 06:30:56 GMT

←Older revision		Revision as of 06:30, 5 July 2023
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	<StructureSection load='5ejn' size='340' side='right'caption='[[5ejn]], [[Resolution\|resolution]] 2.70Å' scene=''>		<StructureSection load='5ejn' size='340' side='right'caption='[[5ejn]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5ejn]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Lk3_transgenic_mice Lk3 transgenic mice~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EJN OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5EJN FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5ejn]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EJN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5EJN FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=NAG:N~~-~~ACETYL-D-GLUCOSAMINE'>NAG</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.703Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~Izumo1r, Folbp3, Folr4, Juno ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode~~=~~Info&srchmode~~=~~5&id=10090 LK3 transgenic mice])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5ejn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ejn OCA], [~~http~~://pdbe.org/5ejn PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5ejn RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5ejn PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5ejn ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ejn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ejn OCA], [https://pdbe.org/5ejn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ejn RCSB], [https://www.ebi.ac.uk/pdbsum/5ejn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ejn ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/JUNO_MOUSE JUNO_MOUSE]] Receptor for IZUMO1 present at the cell surface of oocytes (oolemma), which is essential for gamete recognition and fertilization. The IZUMO1:IZUMO1R/JUNO interaction is a necessary adhesion event between sperm and egg that is required for fertilization but is not sufficient for cell fusion. The ligand-receptor interaction probably does not act as a membrane 'fusogen'. Does not bind folate.<ref>PMID:24739963</ref>	+	[https://www.uniprot.org/uniprot/JUNO_MOUSE JUNO_MOUSE] Receptor for IZUMO1 present at the cell surface of oocytes (oolemma), which is essential for gamete recognition and fertilization. The IZUMO1:IZUMO1R/JUNO interaction is a necessary adhesion event between sperm and egg that is required for fertilization but is not sufficient for cell fusion. The ligand-receptor interaction probably does not act as a membrane 'fusogen'. Does not bind folate.<ref>PMID:24739963</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Lk3 transgenic mice~~]]	+	[[Category: Mus musculus]]
-	[[Category: Han, L]]	+	[[Category: Han L]]
-	[[Category: Jovine, L]]	+	[[Category: Jovine L]]
-	[[Category: Nishimura, K]]	+	[[Category: Nishimura K]]
-	~~[[Category: Cell adhesion]]~~	+
-	~~[[Category: Egg-sperm membrane fusion]]~~	+
-	~~[[Category: Fertilization]]~~	+
-	~~[[Category: Gamete adhesion]]~~	+
-	~~[[Category: Sperm receptor~~]]	+

OCA at 07:01, 22 April 2020

OCA — Wed, 22 Apr 2020 07:01:39 GMT

←Older revision		Revision as of 07:01, 22 April 2020
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	==Crystal structure of Juno, the mammalian egg receptor for sperm Izumo1==		==Crystal structure of Juno, the mammalian egg receptor for sperm Izumo1==
-	<StructureSection load='5ejn' size='340' side='right' caption='[[5ejn]], [[Resolution\|resolution]] 2.70Å' scene=''>	+	<StructureSection load='5ejn' size='340' side='right'caption='[[5ejn]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5ejn]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EJN OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5EJN FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5ejn]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EJN OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5EJN FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5ejn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ejn OCA], [http://pdbe.org/5ejn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ejn RCSB], [http://www.ebi.ac.uk/pdbsum/5ejn PDBsum]</span></td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Izumo1r, Folbp3, Folr4, Juno ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5ejn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ejn OCA], [http://pdbe.org/5ejn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ejn RCSB], [http://www.ebi.ac.uk/pdbsum/5ejn PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ejn ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Lk3 transgenic mice]]
	[[Category: Han, L]]		[[Category: Han, L]]
	[[Category: Jovine, L]]		[[Category: Jovine, L]]

OCA at 18:02, 26 February 2016

OCA — Fri, 26 Feb 2016 18:02:40 GMT

←Older revision		Revision as of 18:02, 26 February 2016
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-	'''Unreleased structure'''

-	~~The entry~~ 5ejn is ~~ON HOLD~~ ~~until Paper~~ Publication	+	==Crystal structure of Juno, the mammalian egg receptor for sperm Izumo1==
		+	<StructureSection load='5ejn' size='340' side='right' caption='[[5ejn]], [[Resolution\|resolution]] 2.70Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5ejn]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EJN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5EJN FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ejn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ejn OCA], [http://pdbe.org/5ejn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ejn RCSB], [http://www.ebi.ac.uk/pdbsum/5ejn PDBsum]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/JUNO_MOUSE JUNO_MOUSE]] Receptor for IZUMO1 present at the cell surface of oocytes (oolemma), which is essential for gamete recognition and fertilization. The IZUMO1:IZUMO1R/JUNO interaction is a necessary adhesion event between sperm and egg that is required for fertilization but is not sufficient for cell fusion. The ligand-receptor interaction probably does not act as a membrane 'fusogen'. Does not bind folate.<ref>PMID:24739963</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The interaction between egg and sperm is the first necessary step of fertilization in all sexually reproducing organisms. A decade-long search for a protein pair mediating this event in mammals culminated in the identification of the glycosylphosphatidylinositol (GPI)-anchored glycoprotein Juno as the egg plasma membrane receptor of sperm Izumo1 [1,2]. The Juno-Izumo1 interaction was shown to be essential for fertilization since mice lacking either gene exhibit sex-specific sterility, making these proteins promising non-hormonal contraceptive targets [1,3]. No structural information is available on how gamete membranes interact at fertilization, and it is unclear how Juno - which was previously named folate receptor (FR) 4, based on sequence similarity considerations - triggers membrane adhesion by binding Izumo1. Here, we report the crystal structure of Juno and find that the overall fold is similar to that of FRalpha and FRbeta but with significant flexibility within the area that corresponds to the rigid ligand-binding site of these bona fide folate receptors. This explains both the inability of Juno to bind vitamin B9/folic acid [1], and why mutations within the flexible region can either abolish or change the species specificity of this interaction. Furthermore, structural similarity between Juno and the cholesterol-binding Niemann-Pick disease type C1 protein (NPC1) suggests how the modified binding surface of Juno may recognize the helical structure of the amino-terminal domain of Izumo1. As Juno appears to be a mammalian innovation, our study indicates that a key evolutionary event in mammalian reproduction originated from the neofunctionalization of the vitamin B9-binding pocket of an ancestral folate receptor molecule.

-	~~Authors: Nishimura, K~~., Han, L., ~~Jovine~~, L.	+	Divergent evolution of vitamin B9 binding underlies Juno-mediated adhesion of mammalian gametes.,Han L, Nishimura K, Sadat Al Hosseini H, Bianchi E, Wright GJ, Jovine L Curr Biol. 2016 Feb 8;26(3):R100-1. doi: 10.1016/j.cub.2015.12.034. PMID:26859261<ref>PMID:26859261</ref>

-	~~Description:~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category: Unreleased Structures]]~~	+	</div>
-	~~[[Category~~: ~~Nishimura, K]]~~	+	<div class="pdbe-citations 5ejn" style="background-color:#fffaf0;"></div>
-	~~[[Category: Jovine, L]]~~	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Han, L]]		[[Category: Han, L]]
		+	[[Category: Jovine, L]]
		+	[[Category: Nishimura, K]]
		+	[[Category: Cell adhesion]]
		+	[[Category: Egg-sperm membrane fusion]]
		+	[[Category: Fertilization]]
		+	[[Category: Gamete adhesion]]
		+	[[Category: Sperm receptor]]

OCA at 23:06, 27 January 2016

OCA — Wed, 27 Jan 2016 23:06:01 GMT

←Older revision		Revision as of 23:06, 27 January 2016
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5ejn is ON HOLD	+	The entry 5ejn is ON HOLD until Paper Publication

	Authors: Nishimura, K., Han, L., Jovine, L.		Authors: Nishimura, K., Han, L., Jovine, L.

OCA: Protected "5ejn" [edit=sysop:move=sysop]

OCA — Tue, 01 Dec 2015 02:55:54 GMT

Protected "5ejn" [edit=sysop:move=sysop]

←Older revision

Revision as of 02:55, 1 December 2015

OCA: New page: '''Unreleased structure''' The entry 5ejn is ON HOLD Authors: Nishimura, K., Han, L., Jovine, L. Description: Category: Unreleased Structures Category: Nishimura, K [[Category...

OCA — Tue, 01 Dec 2015 02:55:53 GMT

New page: '''Unreleased structure''' The entry 5ejn is ON HOLD Authors: Nishimura, K., Han, L., Jovine, L. Description: Category: Unreleased Structures Category: Nishimura, K [[Category...

New page

'''Unreleased structure'''

The entry 5ejn is ON HOLD

Authors: Nishimura, K., Han, L., Jovine, L.

Description:
[[Category: Unreleased Structures]]
[[Category: Nishimura, K]]
[[Category: Jovine, L]]
[[Category: Han, L]]