5eoq - Revision history

OCA at 09:04, 27 September 2023

OCA — Wed, 27 Sep 2023 09:04:06 GMT

←Older revision		Revision as of 09:04, 27 September 2023
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	<StructureSection load='5eoq' size='340' side='right'caption='[[5eoq]], [[Resolution\|resolution]] 1.95Å' scene=''>		<StructureSection load='5eoq' size='340' side='right'caption='[[5eoq]], [[Resolution\|resolution]] 1.95Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5eoq]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EOQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5EOQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5eoq]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus] and [https://en.wikipedia.org/wiki/Vaccinia_virus_WR Vaccinia virus WR]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EOQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5EOQ FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style~~=~~'overflow: auto; max~~-~~height: 3em;'>~~[[~~5eor~~\|~~5eor~~]]~~</div>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.95Å</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5eoq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5eoq OCA], [https://pdbe.org/5eoq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5eoq RCSB], [https://www.ebi.ac.uk/pdbsum/5eoq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5eoq ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5eoq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5eoq OCA], [https://pdbe.org/5eoq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5eoq RCSB], [https://www.ebi.ac.uk/pdbsum/5eoq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5eoq ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/A27_VACCW A27_VACCW]] Structural protein involved in the envelopment of mature virion (MV) to form the wrapped virion (WV). The wrapping consists of the addition of Golgi membranes to the mature virion. Participates in mature virion (MV) movement within the infected cell. May play an indirect role in MV-cell fusion.<ref>PMID:3806791</ref> <ref>PMID:10640541</ref>	+	[https://www.uniprot.org/uniprot/A27_VACCW A27_VACCW] Structural protein involved in the envelopment of mature virion (MV) to form the wrapped virion (WV). The wrapping consists of the addition of Golgi membranes to the mature virion. Participates in mature virion (MV) movement within the infected cell. May play an indirect role in MV-cell fusion.<ref>PMID:3806791</ref> <ref>PMID:10640541</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	==See Also==		==See Also==
-	*[[~~Antibody~~ 3D structures\|~~Antibody~~ 3D structures]]	+	*[[Monoclonal Antibodies 3D structures\|Monoclonal Antibodies 3D structures]]
	*[[3D structures of non-human antibody\|3D structures of non-human antibody]]		*[[3D structures of non-human antibody\|3D structures of non-human antibody]]
	== References ==		== References ==
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	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Mus musculus]]		[[Category: Mus musculus]]
-	[[Category: ~~Matho, M H~~]]	+	[[Category: Vaccinia virus WR]]
-	[[Category: ~~Zajonc, D M~~]]	+	[[Category: Matho MH]]
-	[[Category: ~~Antibody]]~~	+	[[Category: Zajonc DM]]
-	~~[[Category: Fab]]~~	+
-	~~[[Category: Linear epitope]]~~	+
-	~~[[Category: Neutralizing]]~~	+
-	~~[[Category: Vaccinia virus]]~~	+
-	~~[[Category: Viral protein-immune system complex~~]]	+

OCA at 10:10, 13 April 2022

OCA — Wed, 13 Apr 2022 10:10:21 GMT

←Older revision		Revision as of 10:10, 13 April 2022
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	<StructureSection load='5eoq' size='340' side='right'caption='[[5eoq]], [[Resolution\|resolution]] 1.95Å' scene=''>		<StructureSection load='5eoq' size='340' side='right'caption='[[5eoq]], [[Resolution\|resolution]] 1.95Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5eoq]] is a 3 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EOQ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5EOQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5eoq]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EOQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5EOQ FirstGlance]. <br>
-	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5eor\|5eor]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[5eor\|5eor]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5eoq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5eoq OCA], [~~http~~://pdbe.org/5eoq PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5eoq RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5eoq PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5eoq ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5eoq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5eoq OCA], [https://pdbe.org/5eoq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5eoq RCSB], [https://www.ebi.ac.uk/pdbsum/5eoq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5eoq ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/A27_VACCW A27_VACCW]] Structural protein involved in the envelopment of mature virion (MV) to form the wrapped virion (WV). The wrapping consists of the addition of Golgi membranes to the mature virion. Participates in mature virion (MV) movement within the infected cell. May play an indirect role in MV-cell fusion.<ref>PMID:3806791</ref> <ref>PMID:10640541</ref>	+	[[https://www.uniprot.org/uniprot/A27_VACCW A27_VACCW]] Structural protein involved in the envelopment of mature virion (MV) to form the wrapped virion (WV). The wrapping consists of the addition of Golgi membranes to the mature virion. Participates in mature virion (MV) movement within the infected cell. May play an indirect role in MV-cell fusion.<ref>PMID:3806791</ref> <ref>PMID:10640541</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	==See Also==		==See Also==
	*[[Antibody 3D structures\|Antibody 3D structures]]		*[[Antibody 3D structures\|Antibody 3D structures]]
		+	*[[3D structures of non-human antibody\|3D structures of non-human antibody]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 07:07, 22 April 2020

OCA — Wed, 22 Apr 2020 07:07:55 GMT

←Older revision		Revision as of 07:07, 22 April 2020
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	==Structure of the murine Fab 1G6 bound to the vaccinia virus A27 peptide 31-40==		==Structure of the murine Fab 1G6 bound to the vaccinia virus A27 peptide 31-40==
-	<StructureSection load='5eoq' size='340' side='right' caption='[[5eoq]], [[Resolution\|resolution]] 1.95Å' scene=''>	+	<StructureSection load='5eoq' size='340' side='right'caption='[[5eoq]], [[Resolution\|resolution]] 1.95Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5eoq]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EOQ OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5EOQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5eoq]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EOQ OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5EOQ FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5eor\|5eor]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5eor\|5eor]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5eoq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5eoq OCA], [http://pdbe.org/5eoq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5eoq RCSB], [http://www.ebi.ac.uk/pdbsum/5eoq PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5eoq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5eoq OCA], [http://pdbe.org/5eoq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5eoq RCSB], [http://www.ebi.ac.uk/pdbsum/5eoq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5eoq ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	</div>		</div>
	<div class="pdbe-citations 5eoq" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5eoq" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Antibody 3D structures\|Antibody 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Mus musculus]]		[[Category: Mus musculus]]
	[[Category: Matho, M H]]		[[Category: Matho, M H]]

OCA at 19:13, 10 May 2016

OCA — Tue, 10 May 2016 19:13:22 GMT

←Older revision		Revision as of 19:13, 10 May 2016
Line 9:		Line 9:
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/A27_VACCW A27_VACCW]] Structural protein involved in the envelopment of mature virion (MV) to form the wrapped virion (WV). The wrapping consists of the addition of Golgi membranes to the mature virion. Participates in mature virion (MV) movement within the infected cell. May play an indirect role in MV-cell fusion.<ref>PMID:3806791</ref> <ref>PMID:10640541</ref>		[[http://www.uniprot.org/uniprot/A27_VACCW A27_VACCW]] Structural protein involved in the envelopment of mature virion (MV) to form the wrapped virion (WV). The wrapping consists of the addition of Golgi membranes to the mature virion. Participates in mature virion (MV) movement within the infected cell. May play an indirect role in MV-cell fusion.<ref>PMID:3806791</ref> <ref>PMID:10640541</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Vaccinia virus (VACV) A27 is a target for viral neutralization and part of the Dryvax smallpox vaccine. A27 is one of the three glycosaminoglycan (GAG) adhesion molecules and binds to heparan sulfate. To understand the function of anti-A27 antibodies, especially their protective capacity and their interaction with A27, we generated and subsequently characterized 7 murine monoclonal antibodies (MAbs), which fell into 4 distinct epitope groups (groups I to IV). The MAbs in three groups (groups I, III, and IV) bound to linear peptides, while the MAbs in group II bound only to VACV lysate and recombinant A27, suggesting that they recognized a conformational and discontinuous epitope. Only group I antibodies neutralized the mature virion in a complement-dependent manner and protected against VACV challenge, while a group II MAb partially protected against VACV challenge but did not neutralize the mature virion. The epitope for group I MAbs was mapped to a region adjacent to the GAG binding site, a finding which suggests that group I MAbs could potentially interfere with the cellular adhesion of A27. We further determined the crystal structure of the neutralizing group I MAb 1G6, as well as the nonneutralizing group IV MAb 8E3, bound to the corresponding linear epitope-containing peptides. Both the light and the heavy chains of the antibodies are important in binding to their antigens. For both antibodies, the L1 loop seems to dominate the overall polar interactions with the antigen, while for MAb 8E3, the light chain generally appears to make more contacts with the antigen. IMPORTANCE: Vaccinia virus is a powerful model to study antibody responses upon vaccination, since its use as the smallpox vaccine led to the eradication of one of the world's greatest killers. The immunodominant antigens that elicit the protective antibodies are known, yet for many of these antigens, little information about their precise interaction with antibodies is available. In an attempt to better understand the interplay between the antibodies and their antigens, we generated and functionally characterized a panel of anti-A27 antibodies and studied their interaction with the epitope using X-ray crystallography. We identified one protective antibody that binds adjacent to the heparan sulfate binding site of A27, likely affecting ligand binding. Analysis of the antibody-antigen interaction supports a model in which antibodies that can interfere with the functional activity of the antigen are more likely to confer protection than those that bind at the extremities of the antigen.
		+
		+	Linear Epitopes in Vaccinia Virus A27 Are Targets of Protective Antibodies Induced by Vaccination against Smallpox.,Kaever T, Matho MH, Meng X, Crickard L, Schlossman A, Xiang Y, Crotty S, Peters B, Zajonc DM J Virol. 2016 Apr 14;90(9):4334-45. doi: 10.1128/JVI.02878-15. Print 2016 May 1. PMID:26889021<ref>PMID:26889021</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5eoq" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 15:25, 2 March 2016

OCA — Wed, 02 Mar 2016 15:25:14 GMT

←Older revision		Revision as of 15:25, 2 March 2016
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5eoq is ~~ON HOLD until Paper Publication~~	+	==Structure of the murine Fab 1G6 bound to the vaccinia virus A27 peptide 31-40==
-		+	<StructureSection load='5eoq' size='340' side='right' caption='[[5eoq]], [[Resolution\|resolution]] 1.95Å' scene=''>
-	~~Authors~~: ~~Matho~~, M.H., ~~Zajonc~~, D.M.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5eoq]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EOQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5EOQ FirstGlance]. <br>
-	~~Description~~: ~~Structure~~ of the ~~murine Fab 1G6 bound~~ to the ~~vaccinia virus A27 peptide 31~~-40	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5eor\|5eor]]</td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5eoq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5eoq OCA], [http://pdbe.org/5eoq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5eoq RCSB], [http://www.ebi.ac.uk/pdbsum/5eoq PDBsum]</span></td></tr>
-	[[Category: Matho, M.H]]	+	</table>
-	[[Category: Zajonc, D.M]]	+	== Function ==
		+	[[http://www.uniprot.org/uniprot/A27_VACCW A27_VACCW]] Structural protein involved in the envelopment of mature virion (MV) to form the wrapped virion (WV). The wrapping consists of the addition of Golgi membranes to the mature virion. Participates in mature virion (MV) movement within the infected cell. May play an indirect role in MV-cell fusion.<ref>PMID:3806791</ref> <ref>PMID:10640541</ref>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Mus musculus]]
		+	[[Category: Matho, M H]]
		+	[[Category: Zajonc, D M]]
		+	[[Category: Antibody]]
		+	[[Category: Fab]]
		+	[[Category: Linear epitope]]
		+	[[Category: Neutralizing]]
		+	[[Category: Vaccinia virus]]
		+	[[Category: Viral protein-immune system complex]]

OCA: Protected "5eoq" [edit=sysop:move=sysop]

OCA — Tue, 01 Dec 2015 03:08:42 GMT

Protected "5eoq" [edit=sysop:move=sysop]

←Older revision

Revision as of 03:08, 1 December 2015

OCA: New page: '''Unreleased structure''' The entry 5eoq is ON HOLD until Paper Publication Authors: Matho, M.H., Zajonc, D.M. Description: Structure of the murine Fab 1G6 bound to the vaccinia virus...

OCA — Tue, 01 Dec 2015 03:08:41 GMT

New page: '''Unreleased structure''' The entry 5eoq is ON HOLD until Paper Publication Authors: Matho, M.H., Zajonc, D.M. Description: Structure of the murine Fab 1G6 bound to the vaccinia virus...

New page

'''Unreleased structure'''

The entry 5eoq is ON HOLD until Paper Publication

Authors: Matho, M.H., Zajonc, D.M.

Description: Structure of the murine Fab 1G6 bound to the vaccinia virus A27 peptide 31-40
[[Category: Unreleased Structures]]
[[Category: Matho, M.H]]
[[Category: Zajonc, D.M]]