5ep5 - Revision history

OCA at 08:13, 12 July 2023

OCA — Wed, 12 Jul 2023 08:13:17 GMT

←Older revision		Revision as of 08:13, 12 July 2023
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	<StructureSection load='5ep5' size='340' side='right'caption='[[5ep5]], [[Resolution\|resolution]] 2.90Å' scene=''>		<StructureSection load='5ep5' size='340' side='right'caption='[[5ep5]], [[Resolution\|resolution]] 2.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5ep5]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Staam Staam~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EP5 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5EP5 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5ep5]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus_Mu50 Staphylococcus aureus subsp. aureus Mu50]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EP5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5EP5 FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~>[[4m20\|4m20]], [[5eo5\|5eo5]]</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.9Å</td></tr>
-	~~<tr~~ id~~='gene'><td class~~="~~sblockLbl~~">~~<b>~~[[~~Gene~~\|~~Gene:~~]]~~</b></td><td class="sblockDat">SAV0944 ([http://www~~.~~ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=158878 STAAM])~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ep5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ep5 OCA], [https://pdbe.org/5ep5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ep5 RCSB], [https://www.ebi.ac.uk/pdbsum/5ep5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ep5 ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5ep5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ep5 OCA], [~~http~~://pdbe.org/5ep5 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5ep5 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5ep5 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5ep5 ProSAT]</span></td></tr>	+
	</table>		</table>
-	~~<div style~~=~~"background-color:#fffaf0;">~~	+	== Function ==
-	=~~= Publication Abstract from PubMed~~ ==	+	[https://www.uniprot.org/uniprot/A0A0H3JUJ1_STAAM A0A0H3JUJ1_STAAM]
-	Staphylococcus aureus is the causative agent of many diseases, including meningitis, bacteraemia, pneumonia, food poisoning and toxic shock syndrome. Structural characterization of the PaaI-like thioesterase SAV0944 (SaPaaI) from S. aureus subsp. aureus Mu50 will aid in understanding its potential as a new therapeutic target by knowledge of its molecular details and cellular functions. Here, the recombinant expression, purification and crystallization of SaPaaI thioesterase from S. aureus are reported. This protein initially crystallized with the ligand coenzyme A using the hanging-drop vapour-diffusion technique with condition No. 40 of Crystal Screen from Hampton Research at 296 K. Optimal final conditions consisting of 24% PEG 4000, 100 mM sodium citrate pH 6.5, 12% 2-propanol gave single diffraction-quality crystals. These crystals diffracted to beyond 2 A resolution at the Australian Synchrotron and belonged to space group P12(1)1, with unit-cell parameters a = 44.05, b = 89.05, c = 60.74 A, beta = 100.5 degrees . Initial structure determination and refinement gave an R factor and R(free) of 17.3 and 22.0%, respectively, confirming a positive solution in obtaining phases using molecular replacement.	+
-		+
-	Expression, purification, crystallization and preliminary X-ray analysis of the PaaI-like thioesterase SAV0944 from Staphylococcus aureus.,Khandokar YB, Roman N, Smith KM, Srivastava P, Forwood JK Acta Crystallogr F Struct Biol Commun. 2014 Feb;70(Pt 2):~~244-7. doi:, 10.1107~~/~~S2053230X14000338. Epub 2014 Jan 22. PMID:24637766<ref>PMID:24637766<~~/~~ref>~~	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U~~.S. ~~National Library of Medicine.<br>~~	+
-	</~~div>~~	+
-	~~<div class="pdbe-citations 5ep5" style="background-color:#fffaf0;"></div>~~	+
-	~~== References ==~~	+
-	~~<references~~/>	+
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Staam~~]]	+	[[Category: Staphylococcus aureus subsp. aureus Mu50]]
-	[[Category: Forwood~~, J K~~]]	+	[[Category: Forwood JK]]
-	[[Category: Khandokar~~, Y B~~]]	+	[[Category: Khandokar YB]]
-	[[Category: Srivastava, P]]	+	[[Category: Srivastava P]]
-	~~[[Category: Coenzyme a]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Hypothetical protein sav0944]]~~	+
-	~~[[Category: Staphylococcus aureus]]~~	+
-	~~[[Category: Thioesterase~~]]	+

OCA at 07:08, 22 April 2020

OCA — Wed, 22 Apr 2020 07:08:24 GMT

←Older revision		Revision as of 07:08, 22 April 2020
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		+
	==The crystal structure of the hypothetical protein SAV0944 mutant (Glu47Ala) from Staphylococcus aureus.==		==The crystal structure of the hypothetical protein SAV0944 mutant (Glu47Ala) from Staphylococcus aureus.==
-	<StructureSection load='5ep5' size='340' side='right' caption='[[5ep5]], [[Resolution\|resolution]] 2.90Å' scene=''>	+	<StructureSection load='5ep5' size='340' side='right'caption='[[5ep5]], [[Resolution\|resolution]] 2.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5ep5]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EP5 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5EP5 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5ep5]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Staam Staam]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EP5 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5EP5 FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4m20\|4m20]], [[5eo5\|5eo5]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4m20\|4m20]], [[5eo5\|5eo5]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5ep5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ep5 OCA], [http://pdbe.org/5ep5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ep5 RCSB], [http://www.ebi.ac.uk/pdbsum/5ep5 PDBsum]</span></td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SAV0944 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=158878 STAAM])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5ep5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ep5 OCA], [http://pdbe.org/5ep5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ep5 RCSB], [http://www.ebi.ac.uk/pdbsum/5ep5 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ep5 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Staam]]
	[[Category: Forwood, J K]]		[[Category: Forwood, J K]]
	[[Category: Khandokar, Y B]]		[[Category: Khandokar, Y B]]

OCA: Protected "5ep5" [edit=sysop:move=sysop]

OCA — Tue, 01 Dec 2015 04:37:52 GMT

Protected "5ep5" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:37, 1 December 2015

OCA: New page: ==The crystal structure of the hypothetical protein SAV0944 mutant (Glu47Ala) from Staphylococcus aureus.== == Structural highlights ==
<table><tr><td colspan='2'>[[5ep5]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EP5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5EP5 FirstGlance]. <br>
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4m20|4m20]], [[5eo5|5eo5]]</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ep5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ep5 OCA], [http://pdbe.org/5ep5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ep5 RCSB], [http://www.ebi.ac.uk/pdbsum/5ep5 PDBsum]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Staphylococcus aureus is the causative agent of many diseases, including meningitis, bacteraemia, pneumonia, food poisoning and toxic shock syndrome. Structural characterization of the PaaI-like thioesterase SAV0944 (SaPaaI) from S. aureus subsp. aureus Mu50 will aid in understanding its potential as a new therapeutic target by knowledge of its molecular details and cellular functions. Here, the recombinant expression, purification and crystallization of SaPaaI thioesterase from S. aureus are reported. This protein initially crystallized with the ligand coenzyme A using the hanging-drop vapour-diffusion technique with condition No. 40 of Crystal Screen from Hampton Research at 296 K. Optimal final conditions consisting of 24% PEG 4000, 100 mM sodium citrate pH 6.5, 12% 2-propanol gave single diffraction-quality crystals. These crystals diffracted to beyond 2 A resolution at the Australian Synchrotron and belonged to space group P12(1)1, with unit-cell parameters a = 44.05, b = 89.05, c = 60.74 A, beta = 100.5 degrees . Initial structure determination and refinement gave an R factor and R(free) of 17.3 and 22.0%, respectively, confirming a positive solution in obtaining phases using molecular replacement.

Expression, purification, crystallization and preliminary X-ray analysis of the PaaI-like thioesterase SAV0944 from Staphylococcus aureus.,Khandokar YB, Roman N, Smith KM, Srivastava P, Forwood JK Acta Crystallogr F Struct Biol Commun. 2014 Feb;70(Pt 2):244-7. doi:, 10.1107/S2053230X14000338. Epub 2014 Jan 22. PMID:24637766<ref>PMID:24637766</ref>

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5ep5" style="background-color:#fffaf0;"></div>
== References ==
<references/>
TOC
</StructureSection>
[[Category: Forwood, J K]]
[[Category: Khandokar, Y B]]
[[Category: Srivastava, P]]
[[Category: Coenzyme a]]
[[Category: Hydrolase]]
[[Category: Hypothetical protein sav0944]]
[[Category: Staphylococcus aureus]]
[[Category: Thioesterase]]